請用此 Handle URI 來引用此文件:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/46457
完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 鄧麗珍(Lee-Jene Teng) | |
dc.contributor.author | Hsin-Yi Huang | en |
dc.contributor.author | 黃馨儀 | zh_TW |
dc.date.accessioned | 2021-06-15T05:10:02Z | - |
dc.date.available | 2013-09-13 | |
dc.date.copyright | 2010-09-13 | |
dc.date.issued | 2010 | |
dc.date.submitted | 2010-07-25 | |
dc.identifier.citation | 1. Aboshkiwa, M., B. Al-Ani, G. Coleman, and G. Rowland. 1992. Cloning and physical mapping of the Staphylococcus aureus rplL, rpoB and rpoC genes, encoding ribosomal protein L7/L12 and RNA polymerase subunits βand β'. Journal of General Microbiology 138:1875-1880.
2. Balaban, N., P. Stoodley, C. A. Fux, S. Wilson, J. W. Costerton, and G. Dell'Acqua. 2005. Prevention of staphylococcal biofilm-associated infections by the quorum sensing inhibitor RIP. Clinical Orthopaedics and Related Research:48-54. 3. Bobrov, A. G., O. Kirillina, and R. D. Perry. 2007. Regulation of biofilm formation in Yersinia pestis. Advances in Experimental Medicine and Biology 603:201-210. 4. Boles, B. R., and A. R. Horswill. 2008. agr-Mediated Dispersal of Staphylococcus aureus Biofilms. PLoS Pathogens 4:e1000052. 5. Bowden, G. H. W., and Y. H. Li. 1997. Nutritional Influences on Biofilm Development. Advances in Dental Research 11:81-99. 6. Bronner, S., H. Monteil, and G. Prevost. 2006. Regulation of virulence determinants in Staphylococcus aureus :complexity and applications. FEMS Microbiology Reviews 28:183-200. 7. Byun, J.-S., J.-K. Rhee, N. Kim, J. Yoon, D.-U. Kim, E. Koh, J.-W. Oh, and H.-S. Cho. 2007. Crystal structure of hyperthermophilic esterase EstE1 and the relationship between its dimerization and thermostability properties. BMC Structural Biology 7:47. 8. Cerca, N., R. Oliveira, and J. Azeredo. 2007. Susceptibility of Staphylococcus epidermidis planktonic cells and biofilms to the lytic action of staphylococcus bacteriophage K. Letters in Applied Microbiology 45:313-317. 9. Chatterjee, I., P. Becker, M. Grundmeier, M. Bischoff, G. A. Somerville, G. Peters, B. Sinha, N. Harraghy, R. A. Proctor, and M. Herrmann. 2005. Staphylococcus aureus ClpC Is Required for Stress Resistance, Aconitase Activity, Growth Recovery, and Death. The Journal of Bacteriology 187:4488-4496. 10. Cordwell, S. J., M. R. Larsen, R. T. Cole, and B. J. Walsh. 2002. Comparative proteomics of Staphylococcus aureus and the response of methicillin-resistant and methicillin-sensitive strains to Triton X-100. Microbiology 148:2765-2781. 11. Costerton, J. W., P. S. Stewart, and E. P. Greenberg. 1999. Bacterial Biofilms: A Common Cause of Persistent Infections. Science 284:1318-1322. 12. Davies, D. 2003. Understanding biofilm resistance to antibacterial agents. Nature Reviews Drug Discovery 2:114-122. 13. del Pozo, J. L., and R. Patel. 2007. The challenge of treating biofilm-associated bacterial infections. Clinical Pharmacology & Therapeutics 82:204-209. 14. Dodson, S. E., C. J. Heilman, R. A. Kahn, and A. I. Levey. 2007. Production of antisera using fusion proteins. Current Protocols in Neuroscience Chapter 5:Unit 5.7. 15. Dykxhoorn, D. M., R. St Pierre, and T. Linn. 1996. Synthesis of the beta and beta' subunits of Escherichia coli RNA polymerase is autogenously regulated in vivo by both transcriptional and translational mechanisms. Molecular Microbiology 19:483-493. 16. Fitzpatrick, F., H. Humphreys, and J. P. O'Gara. 2005. The genetics of staphylococcal biofilm formation--will a greater understanding of pathogenesis lead to better management of device-related infection? Clinical Microbiology & Infection 11:967-973. 17. Foley, I., P. Marsh, E. M. Wellington, A. W. Smith, and M. R. Brown. 1999. General stress response master regulator rpoS is expressed in human infection: a possible role in chronicity. Journal of Antimicrobial Chemotherapy 43:164-165. 18. Frees, D., A. Chastanet, S. Qazi, K. Sorensen, P. Hill, T. Msadek, and H. Ingmer. 2004. Clp ATPases are required for stress tolerance, intracellular replication and biofilm formation in Staphylococcus aureus. Molecular Microbiology 54:1445-1462. 19. Furukawa, S., Y. Akiyoshi, G. A. O'Toole, H. Ogihara, and Y. Morinaga. 2010. Sugar fatty acid esters inhibit biofilm formation by food-borne pathogenic bacteria. International Journal of Food Microbiology 138:176-180. 20. Gross, M., S. E. Cramton, F. Gotz, and A. Peschel. 2001. Key role of teichoic acid net charge in Staphylococcus aureus colonization of artificial surfaces. Infection and Immunity 69:3423-3426. 21. Hamer, L., T. M. DeZwaan, M. V. Montenegro-Chamorro, S. A. Frank, and J. E. Hamer. 2001. Recent advances in large-scale transposon mutagenesis. Current Opinion in Chemical Biology 5:67-73. 22. Hecker, M., S. Engelmann, and S. J. Cordwell. 2003. Proteomics of Staphylococcus aureus--current state and future challenges. Journal of Chromatography B 787:179-195. 23. Heilmann, C., G. Thumm, G. S. Chhatwal, J. Hartleib, A. Uekotter, and G. Peters. 2003. Identification and characterization of a novel autolysin (Aae) with adhesive properties from Staphylococcus epidermidis. Microbiology 149:2769-2778. 24. Hengge, R. 2009. Principles of c-di-GMP signalling in bacteria. Nature Reviews Microbiology 7:263-273. 25. Herbert, S., A.-K. Ziebandt, K. Ohlsen, T. Schafer, M. Hecker, D. Albrecht, R. Novick, and F. Gotz. 2010. Repair of Global Regulators in Staphylococcus aureus 8325 and Comparative Analysis with Other Clinical Isolates. Infection and Immunity 78:2877-2889. 26. Huang, C. T., K. D. Xu, G. A. McFeters, and P. S. Stewart. 1998. Spatial patterns of alkaline phosphatase expression within bacterial colonies and biofilms in response to phosphate starvation. Applied and Environmental Microbiology 64:1526-1531. 27. Humble, M. W., A. King, and I. Phillips. 1977. API ZYM: a simple rapid system for the detection of bacterial enzymes. Journal of Clinical Pathology 30:275-277. 28. Ishihama, A., and R. Fukuda. 1980. Autogenous and post-transcriptional regulation of RNA polymerase synthesis. Molecular and Cellular Biochemistry 31:177-196. 29. Ito, A., A. Taniuchi, T. May, K. Kawata, and S. Okabe. 2009. Increased antibiotic resistance of Escherichia coli in mature biofilms. Applied and Environmental Microbiology 75:4093-4100. 30. Jass, J., J. W. Costerton, and H. M. Lappin-Scott. 1995. The effect of electrical currents and tobramycin on Pseudomonas aeruginosa biofilms. Journal of industrial microbiology 15:234-242. 31. Jensen, L. J., M. Kuhn, M. Stark, S. Chaffron, C. Creevey, J. Muller, T. Doerks, P. Julien, A. Roth, M. Simonovic, P. Bork, and C. von Mering. 2009. STRING 8--a global view on proteins and their functional interactions in 630 organisms. Nucl. Acids Res. 37:D412-416. 32. Ji, G., R. Beavis, and R. P. Novick. 1997. Bacterial interference caused by autoinducing peptide variants. Science 276:2027-2030. 33. Kaplan, J. B., C. Ragunath, K. Velliyagounder, D. H. Fine, and N. Ramasubbu. 2004. Enzymatic detachment of Staphylococcus epidermidis biofilms. Antimicrobial agents and chemotherapy 48:2633-2636. 34. Knobloch, J. K., S. Jager, M. A. Horstkotte, H. Rohde, and D. Mack. 2004. RsbU-dependent regulation of Staphylococcus epidermidis biofilm formation is mediated via the alternative sigma factor sigmaB by repression of the negative regulator gene icaR. Infection and Immunity 72:3838-3848. 35. Koenig, R. L., J. L. Ray, S. J. Maleki, M. S. Smeltzer, and B. K. Hurlburt. 2004. Staphylococcus aureus AgrA binding to the RNAIII-agr regulatory region. The Journal of Bacteriology 186:7549-55. 36. Kong, K.-F., C. Vuong, and M. Otto. 2006. Staphylococcus quorum sensing in biofilm formation and infection. International Journal of Medical Microbiology 296:133-139. 37. Lamarche, M. G., B. L. Wanner, S. Crepin, and J. Harel. 2008. The phosphate regulon and bacterial virulence: a regulatory network connecting phosphate homeostasis and pathogenesis. FEMS Microbiology Reviews 32:461-473. 38. Lasa, I., and J. R. Penades. 2006. Bap: a family of surface proteins involved in biofilm formation. Res Microbiol 157:99-107. 39. Lehoux, D. E., F. Sanschagrin, and R. C. Levesque. 2001. Discovering essential and infection-related genes. Current Opinion in Microbiology 4:515-519. 40. Lemos, J. A., M. M. Nascimento, V. K. Lin, J. Abranches, and R. A. Burne. 2008. Global Regulation by (p)ppGpp and CodY in Streptococcus mutans. The Journal of Bacteriology 190:5291-5299. 41. Lemos, J. A. C., T. A. Brown, Jr., and R. A. Burne. 2004. Effects of RelA on Key Virulence Properties of Planktonic and Biofilm Populations of Streptococcus mutans. Infection and Immunity 72:1431-1440. 42. Lewis, K. 2001. Riddle of biofilm resistance. Antimicrobial agents and chemotherapy 45:999-1007. 43. Lowy, F. D. 1998. Staphylococcus aureus Infections. N Engl J Med 339:520-532. 44. Magnusson, L. U., A. Farewell, and T. Nystrom. 2005. ppGpp: a global regulator in Escherichia coli. Trends in Microbiology 13:236-242. 45. Mah, T. F., and G. A. O'Toole. 2001. Mechanisms of biofilm resistance to antimicrobial agents. Trends in Microbiology 9:34-39. 46. Marengo, E., E. Robotti, F. Antonucci, D. Cecconi, N. Campostrini, and P. G. Righetti. 2005. Numerical approaches for quantitative analysis of two-dimensional maps: A review of commercial software and home-made systems. Proteomics 5:654-666. 47. McCann, M. T., B. F. Gilmore, and S. P. Gorman. 2008. Staphylococcus epidermidis device-related infections: pathogenesis and clinical management. Journal of Pharmacy and Pharmacology 60:1551-1571. 48. Merritt, J. H., D. E. Kadouri, and G. A. O'Toole. 2005. Growing and analyzing static biofilms. Current Protocols in Microbiology Chapter 1:Unit 1B.1. 49. Murakami, K., T. Ono, D. Viducic, S. Kayama, M. Mori, K. Hirota, K. Nemoto, and Y. Miyake. 2005. Role for rpoS gene of Pseudomonas aeruginosa in antibiotic tolerance. FEMS Microbiology Letters 242:161-167. 50. Nagata, M., C. Kaito, and K. Sekimizu. 2008. Phosphodiesterase Activity of CvfA Is Required for Virulence in Staphylococcus aureus. Journal of Biological Chemistry 283:2176-2184. 51. Otto, M. 2008. Staphylococcal biofilms. Current Topics in Microbiology and Immunology 322:207-228. 52. Otto, M. 2009. Staphylococcus epidermidis- the 'accidental' pathogen. Nature Reviews Microbiology 7:555-567. 53. Patti, J. M., B. L. Allen, M. J. McGavin, and M. Hook. 1994. MSCRAMM-mediated adherence of microorganisms to host tissues. Annual Review of Microbiology 48:585-617. 54. Pitts, B., A. Willse, G. A. McFeters, M. A. Hamilton, N. Zelver, and P. S. Stewart. 2001. A repeatable laboratory method for testing the efficacy of biocides against toilet bowl biofilms. Journal of Applied Microbiology 91:110-117. 55. Ramey, B. E., and M. R. Parsek. 2005. Growing and analyzing biofilms in fermenters. Current Protocols in Microbiology Chapter 1:Unit 1B.3. 56. Resch, A., S. Leicht, M. Saric, L. Pasztor, A. Jakob, F. Gotz, and A. Nordheim. 2006. Comparative proteome analysis of Staphylococcus aureus biofilm and planktonic cells and correlation with transcriptome profiling. Proteomics 6:1867-1877. 57. Rohde, H., C. Burdelski, K. Bartscht, M. Hussain, F. Buck, M. A. Horstkotte, J. K. Knobloch, C. Heilmann, M. Herrmann, and D. Mack. 2005. Induction of Staphylococcus epidermidis biofilm formation via proteolytic processing of the accumulation-associated protein by staphylococcal and host proteases. Molecular Microbiology 55:1883-1895. 58. Rosenstein, R., and F. Gotz. 2000. Staphylococcal lipases: Biochemical and molecular characterization. Biochimie 82:1005-1014. 59. Saxild, H. H., K. Brunstedt, K. I. Nielsen, H. Jarmer, and P. Nygaard. 2001. Definition of the Bacillus subtilis PurR Operator Using Genetic and Bioinformatic Tools and Expansion of the PurR Regulon with glyA, guaC, pbuG, xpt-pbuX, yqhZ-folD, and pbuO. The Journal of Bacteriology 183:6175-6183. 60. Seidl, K., C. Goerke, C. Wolz, D. Mack, B. Berger-Bachi, and M. Bischoff. 2008. Staphylococcus aureus CcpA Affects Biofilm Formation. Infection and Immunity 76:2044-2050. 61. Shanks, R. M. Q., M. A. Meehl, K. M. Brothers, R. M. Martinez, N. P. Donegan, M. L. Graber, A. L. Cheung, and G. A. O'Toole. 2008. Genetic Evidence for an Alternative Citrate-Dependent Biofilm Formation Pathway in Staphylococcus aureus That Is Dependent on Fibronectin Binding Proteins and the GraRS Two-Component Regulatory System. Infection and Immunity 76:2469-2477. 62. Sifri, Costi D. 2008. Healthcare Epidemiology: Quorum Sensing: Bacteria Talk Sense. Clinical Infectious Diseases 47:1070-1076. 63. Smith, A. W. 2005. Biofilms and antibiotic therapy: Is there a role for combating bacterial resistance by the use of novel drug delivery systems? Advanced Drug Delivery Reviews 57:1539-1550. 64. Somerville, G. A., M. S. Chaussee, C. I. Morgan, J. R. Fitzgerald, D. W. Dorward, L. J. Reitzer, and J. M. Musser. 2002. Staphylococcus aureus Aconitase Inactivation Unexpectedly Inhibits Post-Exponential-Phase Growth and Enhances Stationary-Phase Survival. Infection and Immunity 70:6373-6382. 65. Stanley, N. R., and B. A. Lazazzera. 2004. Environmental signals and regulatory pathways that influence biofilm formation. Molecular Microbiology 52:917-924. 66. Stewart, P. S. 2002. Mechanisms of antibiotic resistance in bacterial biofilms. International Journal of Medical Microbiology 292:107-113. 67. Tuomanen, E., R. Cozens, W. Tosch, O. Zak, and A. Tomasz. 1986. The rate of killing of Escherichia coli by beta-lactam antibiotics is strictly proportional to the rate of bacterial growth. Journal of General Microbiology 132:1297-1304. 68. Valle, J., A. Toledo-Arana, C. Berasain, J.-M. Ghigo, B. Amorena, J. R. Penades, and I. Lasa. 2003. SarA and not σB is essential for biofilm development by Staphylococcus aureus. Molecular Microbiology 48:1075-1087. 69. van Schaik, W., and T. Abee. 2005. The role of sigmaB in the stress response of Gram-positive bacteria -- targets for food preservation and safety. Current Opinion in Biotechnology 16:218-224. 70. Vilain, S., J. M. Pretorius, J. Theron, and V. S. Brozel. 2009. DNA as an Adhesin: Bacillus cereus Requires Extracellular DNA To Form Biofilms. Applied and Environmental Microbiology 75:2861-2868. 71. Vilhelmsson, O., and K. J. Miller. 2002. Synthesis of Pyruvate Dehydrogenase in Staphylococcus aureus Is Stimulated by Osmotic Stress. Applied and Environmental Microbiology 68:2353-2358. 72. Vuong, C., M. Durr, A. B. Carmody, A. Peschel, S. J. Klebanoff, and M. Otto. 2004. Regulated expression of pathogen-associated molecular pattern molecules in Staphylococcus epidermidis: quorum-sensing determines pro-inflammatory capacity and production of phenol-soluble modulins. Cellular Microbiology 6:753-759. 73. Vuong, C., C. Gerke, G. A. Somerville, E. R. Fischer, and M. Otto. 2003. Quorum-Sensing Control of Biofilm Factors in 'Staphylococcus epidermidis'. The Journal of Infectious Diseases 188:706-718. 74. Vuong, C., J. B. Kidder, E. R. Jacobson, M. Otto, R. A. Proctor, and G. A. Somerville. 2005. Staphylococcus epidermidis Polysaccharide Intercellular Adhesin Production Significantly Increases during Tricarboxylic Acid Cycle Stress. The Journal of Bacteriology 187:2967-2973. 75. Vuong, C., S. Kocianova, J. M. Voyich, Y. Yao, E. R. Fischer, F. R. DeLeo, and M. Otto. 2004. A crucial role for exopolysaccharide modification in bacterial biofilm formation, immune evasion, and virulence. Journal of Biological Chemistry 279:54881-54886. 76. Vuong, C., S. Kocianova, Y. Yao, Aaron B. Carmody, and M. Otto. 2004. Increased Colonization of Indwelling Medical Devices by Quorum‐Sensing Mutants of Staphylococcus epidermidis In Vivo. The Journal of Infectious Diseases 190:1498-1505. 77. Wang, C., M. Li, D. Dong, J. Wang, J. Ren, M. Otto, and Q. Gao. 2007. Role of ClpP in biofilm formation and virulence of Staphylococcus epidermidis. Microbes and Infection 9:1376-1383. 78. Wu, J. A., C. Kusuma, J. J. Mond, and J. F. Kokai-Kun. 2003. Lysostaphin disrupts Staphylococcus aureus and Staphylococcus epidermidis biofilms on artificial surfaces. Antimicrobial Agents and Chemotherapy 47:3407-3414. 79. Xu, K. D., M. J. Franklin, C.-H. Park, G. A. McFeters, and P. S. Stewart. 2001. Gene expression and protein levels of the stationary phase sigma factor, RpoS, in continuously-fed Pseudomonas aeruginosa biofilms. FEMS Microbiology Letters 199:67-71. 80. 胡俊民. 2008. 脂解酵素基因可能參與金黃色葡萄球菌之生物膜形成. 國立台灣大學碩士論文. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/46457 | - |
dc.description.abstract | 金黃色葡萄球菌(Staphylococcus aureus)為院內感染的主要致病菌之一,造成如導管、人工瓣膜的醫療器材感染等,且多與細菌在器材表面形成生物膜有關。生物膜為細菌的一種群體生活結構,其在生理特性與毒性等等皆與個體有不同的機制表現,導致更高的抗藥性及抵抗免疫的能力,造成治療上的困難。牽涉生物膜形成之相關因子非只有單一因子,在目前的研究中已經發現不少與金黃色葡萄球菌生物膜形成相關的基因,如ica、aap等;或是細菌的調控系統,如agr、sarA、σB等,會對不同的生物膜生成階段造成影響,然而仍未全盤瞭解生物膜生長機制。故本實驗室先前利用突變株庫的建立,篩選其中生物膜生成能力受影響之菌株,從中探討其造成缺陷的原因,以求對生物膜形成機轉更進一步認識。挑選其中一株生物膜生成能力明顯下降之菌株,經由野生株建構與其比較,發現突變株吸附表面的能力受到影響,但於動態生物膜生長並無與野生株不同。此突變株主要帶有putative esterase基因(est)的缺失,故利用API-ZYM套組測定突變株與野生株19種酵素活性,發現突變株脂解酵素能力略有上升。由於此段缺陷基因轉錄之蛋白功能及生理意義目前仍不清楚,無法明瞭其表型與此基因缺陷之關聯性,利用二維電泳及質譜進行蛋白質體分析,藉由鑑定兩株菌株間明顯差異表現之蛋白身份,以生物資訊學工具推測可能影響生物膜形成力路徑。另外亦建構缺陷基因多株抗體以求證實此段基因確實具有影響生物膜生成能力。 | zh_TW |
dc.description.abstract | Staphylococcus aureus is one of pathogens with nosocomial infections. It causes many medical device-related infections which are associated with biofilm formation. These infections are hard to cure completely since biofilms are multicellular microorganisms, presenting different physiological characteristic and virulence, such as increased resistance to antibiotic and host defense. There are many genes which have been reported in involving biofilm formation, like ica, cap;or regulator systems, including agr, sarA, σ B, which influenced diverse phases of biofilm formation. However, many mechanisms are still remaining unclear.In a previous study, a transposon mutant library was generated and tested for the biofilm activity. One mutant strain with significantly lower activity of biofilm formation was obtained, with a putative esterase gene (est) disrupted by transposon.After constructed its wild type and compared with the mutant strain and complementary strains. There was no significant difference of all in flow phase of biofilm formation. Mutant strain had weaker attachment activity to form biofilm. There were two enzyme activities which were associated with lipase increased by using the API-ZYM kit to detected 19 enzyme activities.Due to the unknown function and biological significance of this disrupted gene, we used proteomic approach by 2D electrophoresis and mass spectrographic to search the proteins with different expression level compared to wild type to the mutant strain. Using bioinformatics tools, a possible pathway which may be correlated with biofilm activity was proposed. Furthermore, we constructed the Est poly-colonal antibody to verify the decreased biofilm phenotype cause of est gene disruption. | en |
dc.description.provenance | Made available in DSpace on 2021-06-15T05:10:02Z (GMT). No. of bitstreams: 1 ntu-99-R97424003-1.pdf: 4303029 bytes, checksum: f433a51e96ea98cfcdb7c0b7312d4be1 (MD5) Previous issue date: 2010 | en |
dc.description.tableofcontents | 口試委員會審定書 i
致謝 ii 中文摘要 iii Abstract iv 目錄 1 第一章、 緒論 5 第一節. 簡介 5 第二節. 生物膜(Biofilm) 5 第三節. 跳躍基因致突變法 (Transposon mutagenesis) 11 第四節. 研究目的 11 第二章、 材料與方法 13 第一節. 菌株及質體 13 第二節. 實驗方法 13 2.1 建構野生株 (Wild type) 13 2.2 測定生長曲線 17 2.3 生物膜微量盤試驗 18 2.4 Air-liquid interface assay (ALI) 18 2.5 Rotating disk reactor生物膜培養 19 2.6 API-ZYM 酵素活性反應套組 20 2.7 蛋白質一維電泳分析 21 2.8 蛋白質體研究 22 2.9 多株抗體製備 24 第三章、 實驗結果 35 第一節. 野生株重建 (去質體) 35 第二節. 生長曲線 35 第三節. 生物微量盤試驗 (Microtiter plate assay) 35 第四節. Air-liquid interface assay 35 第五節. Rotating disk reactor生物膜培養 36 第六節. API-ZYM套組測定酵素活性反應 36 第七節. 一維蛋白電泳分析 36 第八節. 蛋白質體分析 37 8.1 二維蛋白電泳 37 8.2 質譜分析鑑定 37 8.3 蛋白交互作用分析 38 第九節. 建構anti-Est多株抗體 39 第十節. 抗體效果測試 39 第四章、 討論 40 第一節. 野生株建構 40 第二節. 生物膜形成缺陷機制探討 40 2.1 生物膜生長培養與分析 40 2.2 酵素活性 42 2.3 蛋白體學研究 43 2.4 小結 47 第三節. 建構之單株抗體成效 47 第五章、 實驗附圖表 49 第六章、 參考文獻 66 | |
dc.language.iso | zh-TW | |
dc.title | 造成生物膜生成缺陷之金黃葡萄球菌突變株機制探討 | zh_TW |
dc.title | Factors involved in a Staphylococcus aureus mutant strain with decreased biofilm activity | en |
dc.type | Thesis | |
dc.date.schoolyear | 98-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 賈景山(J.S Chia),廖淑貞,俞松良(SL Yu) | |
dc.subject.keyword | 金黃色葡萄球菌,生物膜,蛋白二維電泳,質譜分析,酯解酵素, | zh_TW |
dc.subject.keyword | Staphylococcus aureus,biofilm,2-DE,MALDI-TOF,esterase, | en |
dc.relation.page | 72 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2010-07-26 | |
dc.contributor.author-college | 醫學院 | zh_TW |
dc.contributor.author-dept | 醫學檢驗暨生物技術學研究所 | zh_TW |
顯示於系所單位: | 醫學檢驗暨生物技術學系 |
文件中的檔案:
檔案 | 大小 | 格式 | |
---|---|---|---|
ntu-99-1.pdf 目前未授權公開取用 | 4.2 MB | Adobe PDF |
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。