請用此 Handle URI 來引用此文件：
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/46332完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 張煥宗 | |
| dc.contributor.author | Shih-Ju Chen | en |
| dc.contributor.author | 陳詩茹 | zh_TW |
| dc.date.accessioned | 2021-06-15T05:04:02Z | - |
| dc.date.available | 2011-07-28 | |
| dc.date.copyright | 2010-07-28 | |
| dc.date.issued | 2010 | |
| dc.date.submitted | 2010-07-27 | |
| dc.identifier.citation | Ch 1
(1) Famulok, M.; Hartig, J. S.; Mayer, G. Chem. Rev. 2007, 107, 3715. (2) Dubertret, B.; Calame, M.; Libchaber, A. J. Nat. Biotechnol. 2001, 19, 365. (3) Huang, C. C.; Huang, Y. F.; Cao, Z.; Tan, W.; Chang, H. T. Anal. Chem. 2005, 77, 5735. (4) Pavlov, V.; Xiao, Y.; Shlyahovsky, B.; Willner, I. J. Am. Chem. Soc. 2004, 126, 11768. (5) Liu, J.; Lu, Y. Angew. Chem., Int. Ed. 2006, 45, 90. (6) Liu, J.; Lu, Y. J. Am. Chem. Soc. 2004, 126, 12298. (7) Huang, C. C.; Chang, H. T. Chem. Commun. 2008, 2008, 1461. (8) Liu, C. W.; Hsieh, Y. T.; Huang, C. C.; Lina, Z. H.; Chang, H. T. Chem. Commun. 2008, 2244. (9) Gold, L.; Polisky, B.; Uhlenbeck, O.; Yarus, M. Annu. Rev. Biochem. 1995, 64, 763. (10) Wilson, D. S.; Szostak, J. W. Annu. Rev. Biochem. 1999, 68, 611. (11) Hermann, T.; Patel, D. J. Science 2000, 287, 820. (12) Jiang, F.; Kumar, R. A.; Jones, R. A.; Patel, D. J. Nature 1996, 382, 183-186. (13) Juskowiak, B. Anal. Chim. Acta. 2006, 568, 171. (14) Nagatoishi, S.; Nojima, T.; Galezowska, E.; Gluszynska, A.; Juskowiak, B.; Takenaka, S. Anal. Chim. Acta. 2007, 581, 125. (15) Elghanian, R.; Storhoff, J. J.; Mucic, R. C.; Letsinger, R. L.; Mirkin, C. A. Science 1997, 277, 1078. (16) Rupcich, N.; Nutiu, R.; Li, Y.; Brennan, J. D. Anal. Chem. 2005, 77, 4300. (17) Taton, T. A.; Mirkin, C. A.; Letsinger, R. L. Science 2000, 289, 1757. (18) Cao, Y. C.; Jin, R.; Mirkin, C. A. Science 2002, 297, 1536. (19) Willner, I.; Zayats, M. Angew. Chem., Int. Ed. 2007, 46, 6408. (20) Liu, J.; Cao, Z.; Lu, Y. Chem. Rev. 2009, 109, 1948. (21) Stojanovic, M. N.; Landry, D. W. J. Am. Chem. Soc. 2002, 124, 9678. (22) Ho, H. A.; Leclerc, M. J. Am. Chem. Soc. 2004, 126, 1384. (23) McQuade, D. T.; Pullen, A. E.; Swager, T. M. Chem. Rev. 2000, 100, 2537. (24) Ho, H. A.; Bera-Aberem, M.; Leclerc, M. Chem. Eur. J. 2005, 11, 1718. (25) Jin, R.; Wu, G.; Li, Z.; Mirkin, C. A.; Schatz, G. C. J. Am. Chem. Soc. 2003, 125, 1643. (26) Mirkin, C. A.; Letsinger, R. L.; Mucic, R. C.; Storhoff, J. J. Nature 1996, 607. (27) Joseph, M. J.; Taylor, J. C.; McGown, L. B.; Pitner, J. B.; Linn, C. P. Biospectroscopy 1996, 2, 173. (28) Zhou, C.; Jiang, Y.; Hou, S.; Ma, B.; Fang, X.; Li, M. Anal. Bioanal. Chem. 2006, 384, 1175. (29) Wood Iv, A. E.; Bishop, G. R. Molecules 2004, 9, 67. (30) Chiang, C. K.; Huang, C. C.; Liu, C. W.; Chang, H. T. Anal. Chem. 2008, 80, 3716. (31) Tyagi, S.; Bratu, D. P.; Kramer, F. R. Nat. Biotechnol. 1998, 16, 49. (32) Zhang, P.; Beck, T.; Tan, W. Angew. Chem., Int. Ed. 2001, 40, 402. (33) Li, J. J.; Fang, X.; Schuster, S. M.; Tan, W. Angew. Chem., Int. Ed. 2000, 39, 1049. (34) Liu, C. W.; Huang, C. C.; Chang, H. T. Anal. Chem. 2009, 81, 2383. (35) Nutiu, R.; Li, Y. J. Am. Chem. Soc. 2003, 125, 4771. (36) Kandadai, S. A.; Chiuman, W.; Li, Y. Chem. Commun. 2006, 2359. (37) Kamat, P. V.; Barazzouk, S.; Hotchandani, S. Angew. Chem., Int. Ed. 2002, 41, 2764. (38) Huang, C. C.; Chiu, S. H.; Huang, Y. F.; Chang, H. T. Anal. Chem. 2007, 79, 4798. (39) Upadhyay, P.; Hanif, M.; Bhaskar, S. Clin. Microbiol. Infect. 2006, 12, 1118. (40) Smith, J. E.; Medley, C. D.; Tang, Z.; Shangguan, D.; Lofton, C.; Tan, W. Anal. Chem. 2007, 79, 3075. (41) Huang, Y. F.; Chang, H. T.; Tan, W. Anal. Chem. 2008, 80, 567. (42) Huang, C. C.; Chen, C. T.; Shiang, Y. C.; Lin, Z. H.; Chang, H. T. Anal. Chem. 2009, 81, 875. (43) Famulok, M.; Blind, M.; Mayer, G. Chemistry & Biology 2001, 8, 931. (44) Shi, H.; Hoffman, B. E.; Lis, J. T. Proc. Nati. Acad. Sci. 1999, 96, 10033. (45) Lytton-Jean, A. K. R.; Mirkin, C. A. J. Am. Chem. Soc. 2005, 127, 12754. Ch 2 (1) Kim, Y.; Johnson, R. C.; Hupp, J. T. Nano Lett. 2001, 1, 165. (2) Huang, C. C.; Huang, Y. F.; Cao, Z.; Tan, W.; Chang, H. T. Anal. Chem. 2005, 77, 5735. (3) Elghanian, R.; Storhoff, J. J.; Mucic, R. C.; Letsinger, R. L.; Mirkin, C. A. Science 1997, 277, 1078. (4) Nam, J. M.; Park, S. J.; Mirkin, C. A. J. Am. Chem. Soc 2002, 124, 3820. (5) Shenton, W.; Davis, S. A.; Mann, S. Adv. Mater. 1999, 11, 449. (6) Tuerk, C.; Gold, L. Science 1990, 249, 505. (7) Wang, L.; Liu, X.; Hu, X.; Song, S.; Fan, C. Chem. Commu. 2006, 2006, 3780. (8) Li, Y. Y.; Zhang, C.; Li, B. S.; Zhao, L. F.; Li, X.; Yang, W. J.; Xu, S. Q. Clin. Chem. 2007, 53, 1061. (9) Wu, Z. S.; Guo, M. M.; Zhang, S. B.; Chen, C. R.; Jiang, J. H.; Shen, G. L.; Yu, R. Q. Anal. Chem. 2007, 79, 2933. (10) Perez-Ruiz, T.; Martinez-Lozano, C.; Tomas, V.; Martin, J. Anal. Bioanal. Chem. 2003, 377, 189. (11) Jhaveri, S. D.; Kirby, R.; Conrad, R.; Maglott, E. J.; Bowser, M.; Kennedy, R. T.; Glick, G.; Ellington, A. D. J. Am. Chem. Soc. 2000, 122, 2469. (12) Michaud, M.; Jourdan, E.; Ravelet, C.; Villet, A.; Ravel, A.; Grosset, C.; Peyrin, E. Anal. Chem. 2004, 76, 1015. (13) Yoshida, W.; Sode, K.; Ikebukuro, K. Anal. Chem. 2006, 78, 3296. (14) Huang, Y. F.; Chang, H. T. Anal. Chem. 2007, 79, 4852. (15) Lai, R. Y.; Plaxco, K. W.; Heeger, A. J. Anal. Chem. 2007, 79, 229. (16) Liu, J.; Lu, Y. Angew. Chem., Int. Ed. 2006, 45, 90. (17) Zhao, W.; Chiuman, W.; Brook, M. A.; Li, Y. ChemBioChem 2007, 8, 727. (18) Huizenga, D. E.; Szostak, J. W. Biochemistry 1995, 34, 656. (19) Lin, C. H.; Patei, D. J. J. Chem. Biol. 1997, 4, 817. (20) Storhoff, J. J.; Elghanian, R.; Mucic, R. C.; Mirkin, C. A.; Letsinger, R. L. J. Am. Chem. Soc 1998, 120, 1959. (21) Wang, J.; Jiang, Y.; Zhou, C.; Fang, X. Anal. Chem. 2005, 77, 3542. (22) Bowen, B. P.; Enderlein, J.; Woodbury, N. W. Photochem. Photobiol. 2003, 78, 576. (23) Mecklenburg, M.; Grauers, A.; Jonsson, B. R.; Weber, A.; Danielsson, B. Anal. Chim. Acta 1997, 347, 79. (24) Joseph, M. J.; Taylor, J. C.; McGown, L. B.; Pitner, J. B.; Linn, C. P. Biospectroscopy 1996, 2, 173. (25) Vasilevskaya, V. V.; Khokhlov, A. R.; Matsuzawa, Y.; Yoshikawa, K. J. Chem. Phys. 1995, 102, 6595. (26) Miyoshi, D.; Matsumura, S.; Nakano, S.; Sugimoto, N. J. Am. Chem. Soc. 2004, 126, 165. (27) Vallon, V.; Muhlbauer, B.; Osswald, H. Physiol. Rev. 2006, 86, 901. (28) Taniai, H.; Sumi, S.; Ito, T.; Ueta, A.; Ohkubo, Y.; Togari, H. Tohoku J. Exp. Med. 2006, 208, 57. Ch 3 (1) Tuerk, C.; Gold, L. Science 1990, 249, 505. (2) Ellington, A. D.; Szostak, J. W. Nature 1990, 346, 818. (3) Navani, N. K.; Li, Y. Curr. Opin. Chem. Biol. 2006, 10, 272. (4) Huang, C. C.; Chang, H. T. Chem. Commu. 2008, 2008, 1461. (5) Nagatoishi, S.; Nojima, T.; Galezowska, E.; Gluszynska, A.; Juskowiak, B.; Takenaka, S. Anal. Chim. Acta. 2007, 581, 125. (6) Ray, P. C.; Darbha, G. K.; Ray, A.; Walker, J.; Hardy, W. Plasmonics 2007, 2, 173. (7) Huang, Y. C.; Ge, B.; Sen, D.; Yu, H. Z. J. Am. Chem. Soc. 2008, 130, 8023. (8) Vallon, V.; Muhlbauer, B.; Osswald, H. Physiol. Rev. 2006, 86, 901. (9) Siragy, H. M.; Linden, J. Hypertension 1996, 27, 404. (10) Goyal, R. N.; Gupta, V. K.; Chatterjee, S. Talanta 2008, 76, 662. (11) Luippold, G.; Delabar, U.; Kloor, D.; Muhlbauer, B. J. Chromatogr. B 1999, 724, 231. (12) Liu, J.; Lu, Y. Adv. Mater. 2006, 18, 1667. (13) Zhao, W.; Chiuman, W.; Brook, M. A.; Li, Y. ChemBioChem 2007, 8, 727. (14) Chen, S. J.; Huang, Y. F.; Huang, C. C.; Lee, K. H.; Lin, Z. H.; Chang, H. T. Biosens. Bioelectron. 2008, 23, 1749. (15) Juskowiak, B. Anal. Chim. Acta. 2006, 568, 171. (16) Tang, Z.; Mallikaratchy, P.; Yang, R.; Kim, Y.; Zhu, Z.; Wang, H.; Tan, W. J. Am. Chem. Soc. 2008, 130, 11268. (17) Zhang, S.; Xia, J.; Li, X. Anal. Chem. 2008, 80, 8382. (18) Wu, Z. S.; Guo, M. M.; Zhang, S. B.; Chen, C. R.; Jiang, J. H.; Shen, G. L.; Yu, R. Q. Anal. Chem. 2007, 79, 2933. (19) Zayats, M.; Huang, Y.; Gill, R.; Ma, C. A.; Willner, I. J. Am. Chem. Soc. 2006, 128, 13666. (20) Nutiu, R.; Li, Y. J. Am. Chem. Soc. 2003, 125, 4771. (21) Rupcich, N.; Nutiu, R.; Li, Y.; Brennan, J. D. Anal. Chem. 2005, 77, 4300. (22) Nutiu, R.; Li, Y. Methods 2005, 37, 16. (23) Levy, M.; Cater, S. F.; Ellington, A. D. ChemBioChem 2005, 6, 2163. (24) Wang, J.; Jiang, Y.; Zhou, C.; Fang, X. Anal. Chem. 2005, 77, 3542. (25) He, F.; Tang, Y.; Wang, S.; Li, Y.; Zhu, D. J. Am. Chem. Soc. 2005, 127, 12343. (26) Wang, Y.; Liu, B. Analyst 2008, 133, 1593. (27) Wei, H.; Li, B.; Li, J.; Wang, E.; Dong, S. Chem. Commun. 2007, 3735. (28) Ho, H. A.; Leclerc, M. J. Am. Chem. Soc. 2004, 126, 1384. (29) Du, Y.; Li, B.; Wei, H.; Wang, Y.; Wang, E. Anal. Chem. 2008, 80, 5110. (30) Wang, J.; Zhou, H. S. Anal. Chem. 2008, 80, 7174. (31) Huizenga, D. E.; Szostak, J. W. Biochemistry 1995, 34, 656. (32) Lin, C. H.; Patei, D. J. J. Chem. Biol. 1997, 4, 817. (33) Huang, C. C.; Chiu, S. H.; Huang, Y. F.; Chang, H. T. Anal. Chem. 2007, 79, 4798. (34) Huang, Y. F.; Chang, H. T. Anal. Chem. 2006, 78, 1485. (35) Kamat, P. V.; Barazzouk, S.; Hotchandani, S. Angew. Chem., Int. Ed. 2002, 41, 2764. (36) Dulkeith, E.; Morteani, A. C.; Niedereichholz, T.; Klar, T. A.; Feldmann, J.; Levi, S. A.; Van Veggel, F.; Reinhoudt, D. N.; Moller, M.; Gittins, D. I. Phys. Rev. lett. 2002, 89, 203002. (37) Huang, T.; Murray, R. W. Langmuir 2002, 18, 7077. (38) Haugland, R. P.; Spence, M. T. Z.; Johnson, I. D. In Invitrogen Corp., 10th ed.; Spence, B. M. T. Z., Ed.; Molecular Probes: Carlsbad CA, 2005, pp 357. (39) Roduner, E. Chem. Soc. Rev. 2006, 35, 583. (40) Templeton, A. C.; Wuelfing, W. P.; Murray, R. W. Acc. Chem. Res. 2000, 33, 27. (41) Lee, K. H.; Chen, S. J.; Jeng, J. Y.; Cheng, Y. C.; Shiea, J. T.; Chang, H. T. J. Colloid Interf. Sci. 2007, 307, 340. (42) Green, L. S.; Jellinek, D.; Jenison, R.; Ostman, A.; Heldin, C. H.; Janjic, N. Biochemistry 1996, 35, 14413. (43) Floege, J.; Ostendorf, T.; Janssen, U.; Burg, M.; Radeke, H. H.; Vargeese, C.; Gill, S. C.; Green, L. S.; Janjic, N. Amer. J. Pathol. 1999, 154, 169. (44) Liu, C. W.; Huang, C. C.; Chang, H. T. Langmuir 2008, 24, 8346. (45) Heyne, N.; Benohr, P.; Muhlbauer, B.; Delabar, U.; Risler, T.; Osswald, H. Nephrol. Dial. Transplant. 2004, 19, 2737. (46) Kloor, D.; Yao, K.; Delabar, U.; Osswald, H. Clin. Chem. 2000, 46, 537. (47) Taniai, H.; Sumi, S.; Ito, T.; Ueta, A.; Ohkubo, Y.; Togari, H. Tohoku J. Exp. Med. 2006, 208, 57. Ch 4 (1) Tan, G. T.; Wickramasinghe, A.; Verma, S.; Singh, R.; Hughes, S. H.; Pezzuto, J. M.; Baba, M.; Mohan, P. J. Med. Chem. 1992, 35, 4846. (2) Kensch, O.; Connolly, B. A.; Steinhoff, H. J.; McGregor, A.; Goody, R. S.; Restle, T. J. Biol. Chem. 2000, 275, 18271. (3) Jacobo-Molina, A.; Ding, J.; Nanni, R. G.; Clark, A. D.; Lu, X.; Tantillo, C.; Williams, R. L.; Kamer, G.; Ferris, A. L.; Clark, P. Proc. Nat. Acad. Sci. U.S.A. 1993, 90, 6320. (4) Hirsch, M. S.; Brun-Vezinet, F.; D'Aquila, R. T.; Hammer, S. M.; Johnson, V. A.; Kuritzkes, D. R.; Loveday, C.; Mellors, J. W.; Clotet, B.; Conway, B. JAMA 2000, 283, 2417. (5) Esnouf, R.; Ren, J.; Ross, C.; Jones, Y.; Stammers, D.; Stuart, D. Nat. Struct. Mol. Biol. 1995, 2, 303. (6) Spence, R. A.; Kati, W. M.; Anderson, K. S.; Johnson, K. A. Science 1995, 267, 988. (7) Kebba, A.; Atwine, D.; Mwebaze, R.; Kityo, C.; Nakityo, R.; Peter, M. AIDS Res. Hum. Retroviruses 2002, 18, 1181. (8) Gonzales, M. J.; Machekano, R. N.; Shafer, R. W. The Journal of infectious diseases 2001, 184, 998. (9) Deshpande, A.; Jauvin, V.; Magnin, N.; Pinson, P.; Faure, M.; Masquelier, B.; Aurillac-Lavignolle, V.; Fleury, H. J. AIDS Res. Hum. Retroviruses 2007, 23, 335. (10) Mann, M. J.; Dzau, V. J. J. Clin. Invest. 2000, 106, 1071. (11) Schneider, D. J.; Feigon, J.; Hostomsky, Z.; Gold, L. Biochemistry (Mosc). 1995, 34, 9599. (12) Joshi, P. J.; Fisher, T. S.; Prasad, V. R. Current Drug Targets-Infectious Disorders 2003, 3, 383. (13) Kissel, J. D.; Held, D. M.; Hardy, R. W.; Burke, D. H. Nucleic Acids Res. 2007, 35, 1, 1. (14) Andreola, M. L.; Pileur, F.; Calmels, C.; Ventura, M.; Tarrago-Litvak, L.; Toulme, J. J.; Litvak, S. Biochemistry (Mosc). 2001, 40, 10087. (15) Connelly, J. C.; De Leau, E. S.; Leach, D. R. Nucleic Acids Res. 1999, 27, 1039. (16) Biggins, J. B.; Prudent, J. R.; Marshall, D. J.; Ruppen, M.; Thorson, J. S. Proc. Natl. Acad. Sci. U. S. A. 2000, 97, 13537. (17) Grundemann, D.; Schomig, E. Biotechniques 1996, 21, 898. (18) Steinle, E. D.; Mitchell, D. T.; Wirtz, M.; Lee, S. B.; Young, V. Y.; Martin, C. R. Anal. Chem. 2002, 74, 2416. (19) Cui, Z.; Mumper, R. J. Bioconjugate Chem. 2002, 13, 1319. (20) Seferos, D. S.; Prigodich, A. E.; Giljohann, D. A.; Patel, P. C.; Mirkin, C. A. Nano Lett. 2008, 9, 308. (21) He, X.; Wang, K.; Tan, W.; Liu, B.; Lin, X.; He, C.; Huang, S.; Li, J. J. Am. Chem. Soc.3 2003, 125, 7168. (22) Lytton-Jean, A. K. R.; Mirkin, C. A. J. Am. Chem. Soc. 2005, 127, 12754. (23) Taton, T. A.; Mirkin, C. A.; Letsinger, R. L. Science 2000, 289, 1757. (24) Rosi, N. L.; Giljohann, D. A.; Thaxton, C. S.; Lytton-Jean, A. K. R.; Han, M. S.; Mirkin, C. A. Science 2006, 312, 1027. (25) Metzger, W.; Hermann, T.; Schatz, O.; Le Grice, S. F.; Heumann, H. Proc. Natl. Acad. Sci. U. S. A. 1993, 90, 5909. (26) Baillon, J. G.; Nashed, N. T.; Sayer, J. M.; Jerina, D. M. Proc. Natl. Acad. Sci. U. S. A. 1991, 88, 1014. (27) Schatz, O.; Mous, J.; Le Grice, S. F. J. EMBO J. 1990, 9, 1171. (28) Krebs, J. F.; Kore, A. R. Bioconjugate Chem 2008, 19, 185. (29) Tkachenko, A. G.; Xie, H.; Coleman, D.; Glomm, W.; Ryan, J.; Anderson, M. F.; Franzen, S.; Feldheim, D. L. J. Am. Chem. Soc. 2003, 125, 4700. (30) Mammen, M.; Choi, S. K.; Whitesides, A. Angew. Chem. Int. Ed 1998, 37, 2754. (31) Meneendez, J. A.; Ropero, S.; Barbacid, M. M.; Montero, S.; Solanas, M.; Escrich, E.; Cortes-Funes, H.; Colomer, R. Breast Cancer Res. Treat. 2002, 72, 203. (32) Metifiot, M.; Leon, O.; Tarrago-Litvak, L.; Litvak, S.; Andreola, M. L. Biochimie. 2005, 87, 911. (33) Choi, S. J.; Szoka, F. C. Anal. Biochem. 2000, 281, 95-97. (34) Liu, C. W.; Huang, C. C.; Chang, H. T. Langmuir 2008, 24, 8346. (35) Que-Gewirth, N. S.; Sullenger, B. A. Gene Ther. 2007, 14, 283. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/46332 | - |
| dc.description.abstract | 本篇論文主要利用核酸適合體金奈米粒子(Apt-Au NPs)開發出高選擇性、高靈敏度的生化光學感測器，並探討其於生物分子之感測及酵素活性之抑制等生物分析範疇之應用。首先，結合標的物誘導核酸適合體產生構型改變及金奈米粒子在鹽類環境造成聚集現象的特性，本研究使用Apt-Au NPs為基礎的比色裝置來進行腺苷與其磷酸化衍生物(三磷酸腺苷)之感測，此裝置的靈敏度與選擇性，可藉由調控鹽類濃度、變化金奈米表面結合核酸適合體之密度及添加其他物質等方式來改善。此外，本實驗亦成功地結合腺苷接合核酸適合體(adenosine-binding aptamer, AptAdo)、血小板衍生性生長因子接合核酸適合體(platelet-derived growth factor (PDGF)-binding aptamer, AptPDGF)、金奈米粒子及DNA鍵結染料Oligreen (OG)開發出一種免標記(label-free)的螢光分析感測器，並將其應用於尿液腺苷之偵測。在感測混合液中，AptAdo及AptPDGF分別用於辨識分析物及放大偵測訊號，而由實驗結果得知，使用AptAdo.來增強標的物之濃度，可以得到更佳的偵測極限。最後，為測量Apt-Au NPs對酵素活性的抑制效果，本研究選用了兩種核酸適合體－RT1t49 (Aptpol) 及 ODN 93 (AptRH)－它們分別與人類免疫缺乏病毒反轉錄酶(Human immunodeficiency virus type 1 reverse transcriptase, HIV-1 RT)上的聚合酶端(polymerase site, pol) 及核糖核酸酶端(RNase H site, RH) 作用。實驗結果顯示，聚合酶核酸適合體金奈米粒子(Aptpol-Au NPs)及核糖核酸酶端核酸適合體金奈米粒子(AptRH-Au NPs)對於人類免疫缺乏病毒反轉錄酶之聚合現象有很好的抑制效果，且40Aptpol-Au NPs(表示一個金奈米粒籽表面修飾40 條Aptpol)在濃蛋白基質(100 μM BSA)中仍有很好的專一性，且具備不受核酸酶作用之穩定度。此外，本研究亦發現同時添加兩種不同核酸適合體，因其彼此間產生增強作用(Synergy)，可觀察到抑制效果的提升，相關研究具有抑制HIV病毒藥物開發之潛力。綜合上述研究結果，Apt-Au NPs確實為在偵側、診斷及疾病治療等方面極具應用價值之生物分析工具。 | zh_TW |
| dc.description.abstract | In this thesis, a highly selective and sensitive optical biosensor using aptamer-modified gold nanoparticles (Apt-Au NPs) was developed for bio-analytical applications, including the determination of biomolecules and the inhibition of enzyme activity. Combinating with the properties of target-induced conformational changes of aptamers and the salt-induced aggregation of Au NPs, a colorimetric approach using Apt-Au NPs had been designed for the determination of adenosine and its phosphorylated derivatives. The sensitivity of this sensing assay could be improved by evaluating the effects of several parameters, such as the ionic strength, the aptamer density on the Au NPs, and the presence of additives. In addition, I also developed a label-free fluorescence analytical sensor—comprising an adenosine-binding aptamer (AptAdo), platelet-derived growth factor (PDGF)-binding aptamer (AptPDGF), Au NPs, and the DNA-binding dye Oligreen (OG)—for the determination of adenosine. In the sensing mixture, AptAdo and AptPDGF are for the recognition of adenosine and for the amplification of fluorescence signal, respectively. The LOD for adenosine could been further lowered when using AptAdo-Au NPs for the enrichment of adenosine. To demonstrate the inhibition of enzymatic activity by Apt-Au NPs, I selected two aptamers, RT1t49 (Aptpol) and ODN 93 (AptRH), that binds to polymerase site (pol) and RNase H site (RH) of Human immunodeficiency virus type 1 reverse transcriptase (HIV-1 RT), respectively. With the advantages of specific, increased inhibition, AptRH-Au NPs and Aptpol-Au NPs were considered as the highly effective inhibitors for HIV-1 RT polymerization. The stability and specificity of the 40Aptpol-Au NPs remain almost the same in the presence of DNase I and BSA, respectively. Moreover, I found that the combination of 40AptRH-Au NPs with Aptpol led to the synergistic inhibition, which hold great potential to be an effective and alternative drug for HIV. Apt-Au NPs indeed could be applied to the bio-analytical tools for the detection, diagnosis and therapeutic of diseases. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-15T05:04:02Z (GMT). No. of bitstreams: 1 ntu-99-D95223022-1.pdf: 1576432 bytes, checksum: 9db72529a6eda18c81ee016861108e14 (MD5) Previous issue date: 2010 | en |
| dc.description.tableofcontents | 中文摘要 III
關鍵詞 IV Abstract V Keywords VI Contents VII Table Contents X Figure Contents X 1 Introduction 1 1.1 Aptamers-based Biosensors 2 1.2 Colorimetric Detection 3 1.2.1 Organic Dye 4 1.2.2 Conjugated Cationic Polymer 4 1.2.3 Au NPs 4 1.3. Fluorescence Detection 5 1.3.1 Intercalation of Dyes 6 1.3.2 Molecular Beacon 6 1.3.3 Structure-switching Aptamer 7 1.3.4 Au NPs 8 1.4 Sensitivity Improvement 8 1.4.1 Color Change 8 1.4.2 Signal Amplification 9 1.4.3 Multivalent Effect 9 1.5 Motive of Research 10 1.6 References 12 2 Colorimetric Determination of Urinary Adenosine Using Aptamer-Modified Gold Nanoparticles 18 2.1 Introduction 19 2.2 Experimental Section 20 2.2.1 Chemicals 20 2.2.2 Synthesis of Au NPs and Apt-Au NPs 21 2.2.3 Apt-Au NP Sensing Assays 21 2.2.4 Preparation of Urine Samples 22 2.3 Results and Discussion 22 2.3.1 Sensing Mechanism 22 2.3.2 Impact of Salt and Aptamer Density on the Stability of Apt-Au NPs 23 2.3.3 Sensitivity improvement 24 2.3.4 Selectivity and Application of 35-Apt-Au NP Sensing Assays 25 2.4 Conclusions 26 2.5 References 27 3 Enrichment and Fluorescence Enhancement of Adenosine Using Aptamer-Gold Nanoparticles, PDGF Aptamer, and Oligreen 37 3.1 Introduction 38 3.2 Experimental Section 40 3.2.1 Chemicals 40 3.2.2 Synthesis of 13-, 32-, and 56-nm Au NPs 40 3.2.3 Fluorescence Detection of Adenosine 41 3.2.4 Preparation of Aptamer-Bound 32-nm Au NPs 42 3.2.5 AptPDGF/AptAdo-Au NPs Sensor for Adenosion Determination 42 3.2.6 Preparation and Analysis of Urine Samples 43 3.3 Results and Discussion 43 3.3.1 Sensing Strategy of OG-AptAdo/Au NP 43 3.3.2 OG-AptAdo/Au NP-Based Sensor for Adenosine 44 3.3.3 Optimizing Conditions 45 3.3.4 Effect of Particle Sizes 47 3.3.5 Sensitivity Improvement of Aptamer-Based Nanosensor 48 3.3.6 Selectivity of OG-AptAdo/Au NP Sensor for Adenosine 50 3.3.7 Urine Analysis 50 3.4 Conclusions 51 3.5 References 52 4 Aptamer-Conjugated Gold Nanoparticles for the Improved Inhibition of HIV-1 Reverse Transcriptase 67 4.1 Introduction 68 4.2 Experimental Section 70 4.2.1 Chemicals 70 4.2.2 Preparation of Apt-Au NPs 71 4.2.3 DNA polymerase assays 72 4.2.4 Inhibition of RT enzymatic activities 72 4.2.5 Stability of Apt-Au NPs 72 4.2.6 AptRH-Au NPs/Aptpol synergy assays 73 4.3 Results and Discussion 74 4.3.1 Apt-Au NPs as HIV-1 RT inhibitor 74 4.3.2 Effect of ligand density on the activity of Apt-Au NPs 76 4.3.3 Stability of Aptamer and Apt-Au NPs 76 4.3.4 Synergistic effect 77 4.4 Conclusions 78 4.5 References 79 Conclusions and Prospects 88 Publications 90 Conferences 91 | |
| dc.language.iso | en | |
| dc.subject | 抑制作用 | zh_TW |
| dc.subject | 核酸適合體 | zh_TW |
| dc.subject | 金奈米粒子 | zh_TW |
| dc.subject | 生物感測器 | zh_TW |
| dc.subject | 腺苷 | zh_TW |
| dc.subject | 三磷酸腺苷 | zh_TW |
| dc.subject | 人類免疫缺乏病毒反轉錄酶 | zh_TW |
| dc.subject | 聚合酶 | zh_TW |
| dc.subject | aptamer | en |
| dc.subject | inhibition | en |
| dc.subject | polymerase | en |
| dc.subject | Human immunodeficiency virus type 1 reverse transcriptase (HIV-1 RT) | en |
| dc.subject | adenosine triphosphate (ATP) | en |
| dc.subject | adenosine | en |
| dc.subject | G-quadruplex | en |
| dc.subject | biosensor | en |
| dc.subject | gold nanoparticles (Au NPs) | en |
| dc.title | 核酸適合體金奈米粒子於生物感測及酵素抑制之應用 | zh_TW |
| dc.title | Aptamer-conjugated Gold Nanoparticles for Biosensing and Enzymatic Inhibition | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 98-2 | |
| dc.description.degree | 博士 | |
| dc.contributor.oralexamcommittee | 劉春櫻,林萬寅,孫毓璋,蔡素珍 | |
| dc.subject.keyword | 核酸適合體,金奈米粒子,生物感測器,腺苷,三磷酸腺苷,人類免疫缺乏病毒反轉錄酶,聚合酶,抑制作用, | zh_TW |
| dc.subject.keyword | aptamer,gold nanoparticles (Au NPs),biosensor,G-quadruplex,adenosine,adenosine triphosphate (ATP),Human immunodeficiency virus type 1 reverse transcriptase (HIV-1 RT),polymerase,inhibition, | en |
| dc.relation.page | 91 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2010-07-28 | |
| dc.contributor.author-college | 理學院 | zh_TW |
| dc.contributor.author-dept | 化學研究所 | zh_TW |
| 顯示於系所單位： | 化學系 | |
文件中的檔案：
| 檔案 | 大小 | 格式 | |
|---|---|---|---|
| ntu-99-1.pdf 未授權公開取用 | 1.54 MB | Adobe PDF |
系統中的文件，除了特別指名其著作權條款之外，均受到著作權保護，並且保留所有的權利。