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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 孫啟光(Chi-Kuang Sun) | |
| dc.contributor.author | Ming-Liang Wei | en |
| dc.contributor.author | 魏銘良 | zh_TW |
| dc.date.accessioned | 2021-05-14T17:44:23Z | - |
| dc.date.available | 2017-07-30 | |
| dc.date.available | 2021-05-14T17:44:23Z | - |
| dc.date.copyright | 2015-07-30 | |
| dc.date.issued | 2015 | |
| dc.date.submitted | 2015-07-29 | |
| dc.identifier.citation | 參考文獻
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Experimental Dermatology, 21: 281–286. doi: 10.1111/j.1600-0625.2012.01451.x Langfelder, K., Streibel, M., Jahn, B., Haase, G. and Brakhage, A. (2003): Biosynthesis of fungal melanins and their importance for human pathogenic fungi , Fungal genetics and biology, 38 (2), pp. 143-158 . Liao YH, Kuo WC, Chou SY, Tsai CS, Lin GL, Tsai MR, Shih YT, Lee GG, and Sun CK (2014). Quantitative analysis of intrinsic skin aging in dermal papillae by in vivo harmonic generation microscopy. Biomedical Optics Express, 5(9), 3266-79. Lin JS (2010). Effect of mycelial extracts on tyrosinase activity inhibition by Ganoderma lucidum. Liu WM (2013). In Vivo quantification of melanin mass density by using third harmonic generation microscopy. Matthews, T. E., Wilson, J. W., Degan, S., Simpson, M. J., Jin, J. Y., Zhang, J. Y., & Warren, W. S. (2011). In vivo and ex vivo epi-mode pump-probe imaging of melanin and microvasculature. 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Action spectra and quantum yields for eumelanin and synthetic melanin. Photochemistry and Photobiology, 39: 69–74. Sharov, A. A., Fessing, M., Atoyan, R., Sharova, T. Y., Haskell-Luevano, C., Weiner, L., … Botchkarev, V. A. (2005). Bone morphogenetic protein (BMP) signaling controls hair pigmentation by means of cross-talk with the melanocortin receptor-1 pathway. Proceedings of the National Academy of Sciences of the United States of America, 102(1), 93–98. doi:10.1073/pnas.0408455102 Slominski, A., Tobin,D.J., Shibahara, S., & Wortsman, J. (2004). Melanin pigmentation in mammalian skin and its hormonal regulation. Physiological Reviews Published 1 October 2004 Vol. 84 no. 4, 1155-1228 DOI: 10.1152/physrev.00044.2003 Staricco RJ, Pinkus H. (1957). Quantitative and qualitative data on the pigment cells of adult human epidermis. J Invest Dermatol 1957;28:33±45 Szabó, G. (1954). The Number of Melanocytes in Human Epidermis. British Medical Journal, 1(4869), 1016–1004.2. Tai SP, Wu Y, Shieh DB, Chen LJ, Lin KJ, Yu CH, Chu SW, Chang CH, Shi XY, Wen YC, Lin KH, Liu TM and Sun CK (2007). Molecular Imaging of Cancer Cells Using Plasmon-Resonant-Enhanced Third-Harmonic-Generation in Silver Nanoparticles. Adv. Mater., 19: 4520–4523. Thong, H.Y., Jee, S.H., Sun, C.C., & Boissy, R.E. (2003). The patterns of melanosome distribution in keratinocytes of human as one determining factor of skin color. British Journal of Dermatology, Volume 149, Issue 3, pages 498–505, September 2003. Toda, K., Pathak, M.A., Parrish, J.A., Fitzpatrick, T.B., & Quevedo, W.C. (1972). Alteration of racial differences in melanosome distribution in human epidermis after exposure to ultraviolet light. Nature new biology 236, 143-145. Tomohiro, I., Yukio F. (2005). Hot-Water Extracts from Adzuki Beans (Vigna angularis) Stimulate Not Only Melanogenesis in Cultured Mouse B16 Melanoma Cells but Also Pigmentation of Hair Color in C3H Mice. Bioscience, Biotechnology, and Biochemistry, 69, 873-882. Tsai MR (2012). Clinical Application of Higher Harmonic Generation Microscopy in Superficial Disease Diagnostics. Tsai MR, Lin CY, Liao YH, Liu HL, and Sun CK (2012). Applying tattoo dye as a third-harmonic generation contrast agent for in vivo optical virtual biopsy of human skin. Journal of Biomedical Optics, 18 (2), 026012. WHO (World Health Organization). (1994). Environmental Health Criteria 160. Ultraviolet radiation. Wolff, K., Fitzpatrick, T. & Goldsmith, L. (2008). Fitzpatrick's dermatology in general medicine. New York: McGraw-Hill, Medical Pub. Division. Zonios, G., Bykowski, J., & Kolloias, N. (2001). Skin melanin, hemoglobin, and light scattering properties can be quantitatively assessed in vivo using diffuse reflectance spectroscopy. Journal of Investigative Dermatology (2001) 117, 1452–1457; doi:10.1046/j.0022-202x.2001.01577.x 王鑄軍，(1992)。臨床皮膚科學。新第四版，台北力大圖書有限公司。 洪偉章，陳榮秀，(1997)。化妝品科技概論。高立出版社，台北，台灣。 曾敏南，陳昱初，(2013)。電子顯微技術應用於微生物學之新發展。行政院農業委員會高雄區農業改良場研究彙報，第24卷第1期。 | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/4632 | - |
| dc.description.abstract | 本研究藉由分析倍頻顯微術影像中三倍頻訊號的放大率，量化肝斑病人體內的黑色素質量密度，並進一步分析黑色素質量密度與肝斑的關聯性。肝斑是一種常見的皮膚疾病，與黑色素沈澱有關。黑色素的數量和分布是決定肝斑治療和癒後的關鍵，因此黑色素量化技術十分重要。倍頻顯微術是一種可作為活體光學虛擬切片的非線性光學顯微術，包括二倍頻訊號與三倍頻訊號。過去的研究顯示，由黑色素造成的三倍頻放大率與黑色素質量密度有明顯的對應關係。因此可藉由分析肝斑病人影像中的三倍頻放大率，量化其體內的黑色素質量密度。
由倍頻顯微術影像中，取得基底細胞三倍頻與膠原蛋白三倍頻訊號強度，以白斑病人影像校正後，可得11位肝斑病人影像的平均黑色素質量密度為 16.7(mg/ml)。在本研究中的理論模型與限制條件之下，顯示肝斑部位 (16.27mg/ml)與對照組 (17.11mg/ml)的黑色素質量密度平均值無顯著差異，但是前者 (6.28%)的三倍頻影像像素飽和比例明顯較後者 (2.85%)高。我們認為像素飽和現象造成了最大值的限制，使得兩組數據皆因此低估，而無法出現顯著差異。此外分析結果亦顯示肝斑部位的表皮層厚度較薄，可能是造成肝斑視覺效果較黑的原因之一；甚至可推論表皮層較薄或變薄，也許是造成肝斑的發生因素或前置警訊。 | zh_TW |
| dc.description.abstract | Melasma is a common skin disease of hyperpigmentation disorder of melanin. Since the quantity and distribution of melanin are the main factors of the therapeutic decision and outcome of melasma, the quantification method of melanin is very important. Harmonic generation microscopy (HGM), including second harmonic generation (SHG) microscopy and third harmonic generation (THG) microscopy, is a nonlinear optical microscopy for in vivo optical virtual biopsy of human skin. In previous studies, the THG enhancement ratio by melanin is obviously related to the melanin mass density, so in this study, we can quantify the melanin mass density in human by the analysis of THG enhancement ratio in third harmonic generation microscopy.
By analyzing the images of HGM, we can get the THG signal intensity of basal cell and collagen. Then we can get the average melanin mass density (16.7mg/ml) of all HGM images from patients with melasma by calibrating with the HGM images from patients with vitiligo. Under the boundary conditions in this study, the difference between the melanin mass density of the melasma group (16.27mg/ml) and control group (17.11mg/ml) is not statistically significant, but the saturated ratio of the THG image in melasma group (6.28%) is obvious higher than the control one (2.85%). The results also show that the epidermis in the melasma group is thinner than the control one, and it may result in the darker visual appeal. It also implies that the thinner skin is one of the cause of melasma. | en |
| dc.description.provenance | Made available in DSpace on 2021-05-14T17:44:23Z (GMT). No. of bitstreams: 1 ntu-104-R00945046-1.pdf: 3780910 bytes, checksum: 3cdf18640a7e9583f21d383cc12e2b74 (MD5) Previous issue date: 2015 | en |
| dc.description.tableofcontents | 目錄
口試委員會審定書 # 誌謝 i 摘要 ii ABSTRACT iii 目錄 iv 圖目錄 viii 表目錄 xi Chapter 1 緒論 1 1.1 肝斑 (melasma) 1 1.1.1 肝斑簡介 1 1.1.2 肝斑成因 2 1.1.3 肝斑的治療 2 1.2 黑色素 (melanin) 4 1.2.1 黑色素分類 4 1.2.2 黑色素組成 4 1.2.3 黑色素合成機制 5 1.2.4 黑色素的功能 (function of melanin) 6 1.3 黑色素與皮膚 (skin) 8 1.3.1 皮膚構造 (structure of the skin) 8 1.3.2 黑色素細胞與黑色素體 (melanocyte and melanosome) 10 1.3.3 皮膚的色素 (pigment) 12 1.3.4 皮膚的色素沉澱 (pigmentation) 12 1.3.5 黑色素的生成與抑制 13 1.3.6 黑色素的缺乏-白斑 (vitiligo) 14 1.4 黑色素量化 (quantification) 15 1.4.1 黑色素量化與皮膚顏色 (skin color) 15 1.4.2 黑色素量化與色素異常 (pigmentation disorder) 15 1.4.3 常見的黑色素量化技術 16 1.4.4 黑色素量化與光學顯微術 (optical microscopy) 17 1.5 非線性光學顯微術與倍頻顯微術 19 1.5.1 非線性光學顯微術 (non-linear optical microscopy) 19 1.5.2 倍頻顯微術 (harmonic generation microscopy) 20 1.5.3 倍頻顯微術與臨床皮膚研究 21 1.6 三倍頻放大現象 (THG enhancement) 23 1.6.1 刺青染劑 (tattoo dye) 23 1.6.2 銀奈米粒子 (silver nanoparticles) 24 1.6.3 黑色素 (melanin) 25 1.7 黑色素質量密度與三倍頻放大率 26 1.7.1 黑色素質量密度 (melanin mass density) 26 1.7.2 三倍頻放大率 (THG enhancement ratio) 26 1.7.3 以倍頻顯微術量化黑色素質量密度 26 Chapter 2 研究方法與過程 29 2.1 研究目的 29 2.2 研究設備 29 2.3 研究對象 31 2.3.1 肝斑病人皮膚影像 31 2.3.2 白斑病人皮膚影像 31 2.3.3 影像格式與分析軟體 32 2.3.4 影像特性 33 2.4 實驗步驟 36 2.4.1 影像挑選 36 2.4.2 背景分析--取得三倍頻背景雜訊 (noise) 42 2.4.3 手動圈選--取得基底細胞三倍頻訊號強度 (THGbasal cell) 42 2.4.4 程式判斷--取得膠原蛋白三倍頻訊號強度 (THGcollagen) 43 2.4.5 白斑影像分析--取得白斑影像三倍頻訊號強度與背景雜訊 45 2.5 理論模型與數據分析 46 2.5.1 理論模型 46 2.5.2 定義變數 47 2.5.3 數據分析 48 Chapter 3 研究結果與討論 51 3.1 影像分析結果與討論 51 3.1.1 肝斑病人影像三倍頻訊號強度 51 3.1.2 肝斑病人影像中基底細胞三倍頻與膠原蛋白三倍頻關係 54 3.1.3 白斑病人影像分析 55 3.1.4 白斑病人影像中基底細胞與膠原蛋白三倍頻訊號比值R 55 3.1.5 肝斑病人影像中三倍頻放大率G(M)與黑色素質量密度M 56 3.2 肝斑臨床分析結果與討論 57 3.2.1 肝斑部位與對照組 57 3.2.2 雷射處理與對照組 59 3.3 延伸討論--篩選限制條件 (boundary conditions) 61 3.3.1 增加每層挑選細胞數目 61 3.3.2 飽和排除比例與像素飽和比例 64 3.3.3 交界層深度 (表皮層厚度) 68 3.3.4 交界層膠原蛋白三倍頻訊號與交界層深度關係 71 3.3.5 真皮層膠原蛋白三倍頻訊號與真皮層深度關係 74 3.3.6 影像最亮層 77 3.4 結論與未來展望 80 3.4.1 結論 80 3.4.2 未來展望 82 參考文獻 83 附錄A 91 | |
| dc.language.iso | zh-TW | |
| dc.title | 利用三倍頻顯微術量化肝斑病人體內黑色素質量密度 | zh_TW |
| dc.title | In Vivo Quantification of Melanin Mass Density in Human
by Using Third Harmonic Generation Microscopy | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 103-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 李國君(Gwo-Giun Lee),廖怡華(Yi-Hua Liao) | |
| dc.subject.keyword | 肝斑,黑色素,量化,倍頻顯微術,三倍頻放大率,黑色素質量密度, | zh_TW |
| dc.subject.keyword | melasma,melanin,quantification,harmonic generation microscopy,THG enhancement ratio,melanin mass density, | en |
| dc.relation.page | 93 | |
| dc.rights.note | 同意授權(全球公開) | |
| dc.date.accepted | 2015-07-29 | |
| dc.contributor.author-college | 電機資訊學院 | zh_TW |
| dc.contributor.author-dept | 生醫電子與資訊學研究所 | zh_TW |
| 顯示於系所單位： | 生醫電子與資訊學研究所 | |
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