請用此 Handle URI 來引用此文件：
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/46249完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 鄧述諄 | |
| dc.contributor.author | Wan-Yu Lee | en |
| dc.contributor.author | 李宛育 | zh_TW |
| dc.date.accessioned | 2021-06-15T04:59:56Z | - |
| dc.date.available | 2020-12-31 | |
| dc.date.copyright | 2010-09-09 | |
| dc.date.issued | 2010 | |
| dc.date.submitted | 2010-07-28 | |
| dc.identifier.citation | 1. Tapon, N., Moberg, K. H., and Hariharan, I. K. (2001) Curr Opin Cell Biol 13(6), 731-737
2. Breeden, L. L. (2003) Curr Biol 13(1), R31-38 3. Wittenberg, C., and La Valle, R. (2003) Bioessays 25(9), 856-867 4. Cho, R. J., Campbell, M. J., Winzeler, E. A., Steinmetz, L., Conway, A., Wodicka, L., Wolfsberg, T. G., Gabrielian, A. E., Landsman, D., Lockhart, D. J., and Davis, R. W. (1998) Mol Cell 2(1), 65-73 5. Spellman, P. T., Sherlock, G., Zhang, M. Q., Iyer, V. R., Anders, K., Eisen, M. B., Brown, P. O., Botstein, D., and Futcher, B. (1998) Mol Biol Cell 9(12), 3273-3297 6. Richardson, H. E., Wittenberg, C., Cross, F., and Reed, S. I. (1989) Cell 59(6), 1127-1133 7. Mendenhall, M. D., and Hodge, A. E. (1998) Microbiol Mol Biol Rev 62(4), 1191-1243 8. Tyers, M., Tokiwa, G., and Futcher, B. (1993) Embo J 12(5), 1955-1968 9. Ghaemmaghami, S., Huh, W. K., Bower, K., Howson, R. W., Belle, A., Dephoure, N., O'Shea, E. K., and Weissman, J. S. (2003) Nature 425(6959), 737-741 10. Hubler, L., Bradshaw-Rouse, J., and Heideman, W. (1993) Mol Cell Biol 13(10), 6274-6282 11. Polymenis, M., and Schmidt, E. V. (1997) Genes Dev 11(19), 2522-2531 12. Futcher, B. (1996) Yeast 12(16), 1635-1646 13. Dirick, L., Bohm, T., and Nasmyth, K. (1995) Embo J 14(19), 4803-4813 14. Koch, C., Moll, T., Neuberg, M., Ahorn, H., and Nasmyth, K. (1993) Science 261(5128), 1551-1557 15. Cross, F. R., Hoek, M., McKinney, J. D., and Tinkelenberg, A. H. (1994) Mol Cell Biol 14(7), 4779-4787 16. Toone, W. M., Aerne, B. L., Morgan, B. A., and Johnston, L. H. (1997) Annu Rev Microbiol 51, 125-149 17. Stuart, D., and Wittenberg, C. (1995) Genes Dev 9(22), 2780-2794 18. Costanzo, M., Nishikawa, J. L., Tang, X., Millman, J. S., Schub, O., Breitkreuz, K., Dewar, D., Rupes, I., Andrews, B., and Tyers, M. (2004) Cell 117(7), 899-913 19. de Bruin, R. A., McDonald, W. H., Kalashnikova, T. I., Yates, J., 3rd, and Wittenberg, C. (2004) Cell 117(7), 887-898 20. Schaefer, J. B., and Breeden, L. L. (2004) Cell 117(7), 849-850 21. Mai, B., Miles, S., and Breeden, L. L. (2002) Mol Cell Biol 22(2), 430-441 22. Pramila, T., Miles, S., GuhaThakurta, D., Jemiolo, D., and Breeden, L. L. (2002) Genes Dev 16(23), 3034-3045 23. Uetz, P., Giot, L., Cagney, G., Mansfield, T. A., Judson, R. S., Knight, J. R., Lockshon, D., Narayan, V., Srinivasan, M., Pochart, P., Qureshi-Emili, A., Li, Y., Godwin, B., Conover, D., Kalbfleisch, T., Vijayadamodar, G., Yang, M., Johnston, M., Fields, S., and Rothberg, J. M. (2000) Nature 403(6770), 623-627 24. Gavin, A. C., Bosche, M., Krause, R., Grandi, P., Marzioch, M., Bauer, A., Schultz, J., Rick, J. M., Michon, A. M., Cruciat, C. M., Remor, M., Hofert, C., Schelder, M., Brajenovic, M., Ruffner, H., Merino, A., Klein, K., Hudak, M., Dickson, D., Rudi, T., Gnau, V., Bauch, A., Bastuck, S., Huhse, B., Leutwein, C., Heurtier, M. A., Copley, R. R., Edelmann, A., Querfurth, E., Rybin, V., Drewes, G., Raida, M., Bouwmeester, T., Bork, P., Seraphin, B., Kuster, B., Neubauer, G., and Superti-Furga, G. (2002) Nature 415(6868), 141-147 25. Teng, S. C., Epstein, C., Tsai, Y. L., Cheng, H. W., Chen, H. L., and Lin, J. J. (2002) Biochem Biophys Res Commun 291(3), 714-721 26. Sikorski, R. S., and Boeke, J. D. (1991) Methods Enzymol 194, 302-318 27. Tseng, S. F., Chang, C. Y., Wu, K. J., and Teng, S. C. (2005) J Biol Chem 280(47), 39594-39600 28. Flick, K. M., Spielewoy, N., Kalashnikova, T. I., Guaderrama, M., Zhu, Q., Chang, H. C., and Wittenberg, C. (2003) Mol Biol Cell 14(8), 3230-3241 29. Taggart, A. K., Teng, S. C., and Zakian, V. A. (2002) Science 297(5583), 1023-1026 30. Koch, C., Schleiffer, A., Ammerer, G., and Nasmyth, K. (1996) Genes Dev 10(2), 129-141 31. Bashir, T., N. V. Dorrello, V. Amador, D. Guardavaccaro And M. Pagano, 2004 Control of the SCF(Skp2-Cks1) ubiquitin ligase by the APC/C(Cdh1) ubiquitin ligase. Nature 428: 190-193. 32. Chen, Q., A. Ijpma And C. W. Greider, 2001 Two survivor pathways that allow growth in the absence of telomerase are generated by distinct telomere recombination events. Mol Cell Biol 21: 1819-1827. 33. Dubrana, K., S. Perrod And S. M. Gasser, 2001 Turning telomeres off and on. Curr Opin Cell Biol 13: 281-289. 34. Gavin, A. C., M. Bosche, R. Krause, P. Grandi, M. Marzioch et al., 2002 Functional organization of the yeast proteome by systematic analysis of protein complexes. Nature 415: 141-147. 35. Hadwiger, J. A., C. Wittenberg, H. E. Richardson, M. De Barros Lopes And S. I. Reed, 1989 A family of cyclin homologs that control the G1 phase in yeast. Proc Natl Acad Sci U S A 86: 6255-6259. 36. Mendenhall, M. D., And A. E. Hodge, 1998 Regulation of Cdc28 cyclin-dependent protein kinase activity during the cell cycle of the yeast Saccharomyces cerevisiae. Microbiol Mol Biol Rev 62: 1191-1243. 37. Morgan, D. O., 1995 Principles of CDK regulation. Nature 374: 131-134. 38. Morris, M. C., P. Kaiser, S. Rudyak, C. Baskerville, M. H. Watson et al., 2003 Cks1-dependent proteasome recruitment and activation of CDC20 transcription in budding yeast. Nature 424: 1009-1013. 39. Nautiyal, S., J. L. Derisi And E. H. Blackburn, 2002 The genome-wide expression response to telomerase deletion in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A 99: 9316-9321. 40. Newcomb, L. L., D. D. Hall And W. Heideman, 2002 AZF1 is a glucose-dependent positive regulator of CLN3 transcription in Saccharomyces cerevisiae. Mol Cell Biol 22: 1607-1614. 41. Pondugula, S., et al., Coupling phosphate homeostasis to cell cycle-specific transcription: mitotic activation of Saccharomyces cerevisiae PHO5 by Mcm1 and Forkhead proteins. Mol Cell Biol, 2009. 29(18): p. 4891-905. 42. Reynard, G. J., W. Reynolds, R. Verma And R. J. Deshaies, 2000 Cks1 is required for G(1) cyclin-cyclin-dependent kinase activity in budding yeast. Mol Cell Biol 20: 5858-5864. 43. Rupes, I., 2002 Checking cell size in yeast. Trends Genet 18: 479-485. 44. Sherlock, G., And J. Rosamond, 1993 Starting to cycle: G1 controls regulating cell division in budding yeast. J Gen Microbiol 139 ( Pt 11): 2531-2541. 45. Singer, M. S., And D. E. Gottschling, 1994 TLC1: template RNA component of Saccharomyces cerevisiae telomerase. Science 266: 404-409. 46. Tang, Y., And S. I. Reed, 1993 The Cdk-associated protein Cks1 functions both in G1 and G2 in Saccharomyces cerevisiae. Genes Dev 7: 822-832. 47. Tyers, M., G. Tokiwa, R. Nash And B. Futcher, 1992 The Cln3-Cdc28 kinase complex of S. cerevisiae is regulated by proteolysis and phosphorylation. Embo J 11: 1773-1784. 48. Tyers, M., G. Tokiwa And B. Futcher, 1993 Comparison of the Saccharomyces cerevisiae G1 cyclins: Cln3 may be an upstream activator of Cln1, Cln2 and other cyclins. Embo J 12: 1955-1968. 49. Uetz, P., L. Giot, G. Cagney, T. A. Mansfield, R. S. Judson et al., 2000 A comprehensive analysis of protein-protein interactions in Saccharomyces cerevisiae. Nature 403: 623-627. 50. Yaglom, J., M. H. Linskens, S. Sadis, D. M. Rubin, B. Futcher et al., 1995 p34Cdc28-mediated control of Cln3 cyclin degradation. Mol Cell Biol 15: 731-741. 51. Yaglom, J. A., A. L. Goldberg, D. Finley And M. Y. Sherman, 1996 The molecular chaperone Ydj1 is required for the p34CDC28-dependent phosphorylation of the cyclin Cln3 that signals its degradation. Mol Cell Biol 16: 3679-3684. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/46249 | - |
| dc.description.abstract | 細胞週期，是指能持續分裂的真核細胞從一次有絲分裂結束後生長，再到下一次分裂結束的循環過程。細胞週期是所有生物繁殖複製所必需的機制，而出芽酵母菌則是用來研究細胞週期調控制很重要的模式生物。真核生物的細胞週期可分為G1、S、G2、M四個時期，其中G1時期的長度受到外在環境及訊息的影響非常大，細胞是否能夠通過G1要進入到S時期此一主要的調控點是由Cdk及G1 週期素(Cyclin)活性所調控。由先前的研究中顯示Ypl014w可能與Cdk1、Cln2等細胞週期調蛋白形成複合體，因此本研究的目的在於探討此蛋白質在酵母菌細胞週期中所執行的功能為何。我們的實驗結果顯示YPL014W受到一轉錄因子Mcm1的調控而有週期性的表現，並且透過免疫沉澱的方法證實其參與在Cln2複合體中。此基因的剔除對細胞週期的進行沒有顯著的影響，但是過度表現此蛋白質會造成細胞週期停滯在G1時期。本實驗證實Ypl014w的確參與在細胞週期的調控之中並且受到Mcm1的調控,而其詳細的機制及調控的方式我們將會更進一步的研究透徹。 | zh_TW |
| dc.description.abstract | The G1-S transition in the cell cycle is largely regulated by the activity of the G1 cyclin-Cdc28 cyclin-dependent protein kinase complex. Among G1 cyclins, Cln1 and Cln2 appear to make a specific contribution to the initiation of the cell cycle, while Cln3 functions for the timely expression of CLN1, CLN2 and other G1-specific genes. The Cln3-Cdc28 kinase activates the Swi4-Swi6 transcription complex which induces CLN1 and CLN2 transcription in late G1 and drives the transition to S phase. Previous results suggest that Ypl014w p is a component of the G1 cyclin/Cdc28 complex. However, it is still elusive how this gene is cell cycle-regulated and whether it affects the activity of the G1 cyclin-CDK complex. The preliminary data showed that YPL014W is activated by the transcription factor, Mcm1p. Chromatin immunoprecipitation experiments demonstrate a direct binding of Mcm1p at the promoter of YPL014W.
In this study, we show that YPL014W is expressed in a cell cycle-regulated pattern. In addition, the interaction between Ypl014w and Cln2 was confirmed by co-immunoprecipitation. Moreover we demonstrated the Ypl014w overexpression cause the cell cycle arrest at G1 phase. These observations support the view that the Mcm1p-coordinated rise of YPL014W level is requires for the cell cycle regulation , the mechanism involved thus need to be further investigated. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-15T04:59:56Z (GMT). No. of bitstreams: 1 ntu-99-R97445132-1.pdf: 2891912 bytes, checksum: 55c2595f8189d8241db29f4486814bb8 (MD5) Previous issue date: 2010 | en |
| dc.description.tableofcontents | 口試委員會審定書 #
誌謝………………………………………………………………………………..……. i 中文摘要 ii ABSTRACT iii CONTENTS iv Chapter 1 Introduction 1 Chapter 2 Materials and Methods 4 2.1 Yeast strains and plasmid constructions 4 2.2 Cell synchrony experiments and FACS analysis 4 2.3 DNA damage sensitivity assays 5 2.4 Reverse transcription and real-time PCR assay 5 2.5 Co-immunoprecipitation 5 Chapter 3 Results 7 Chapter 4 Discussion 13 Chapter 5 Figures 15 Fig. 1 Cell cycle regulation of YPL014W expression Fig. 2 Mcm1p binding sites within the proximal promoter region of the YPL014w gene and ECB-mediated transcriptional activation of the YPL014w promoter Fig. 3 YPL014w is dispensable for the proper cell cycle Fig. 4 Overexpression of YPL014W causes a G1 phase arrest Fig. 5 Ypl014w coimmunoprecipitates with Cln2 but not Cks1 in vivo Fig. 6 Domain mapping studies of the interaction between Ypl014w and Cln2 Fig.7 Slow growth phenotype was examined in strains combined with the YPL014W overexpression. Fig. 8 Viability assay of cln mutations. Fig. 9 Spot assay of ypl014w strain to DNA-damaging agents TABLES……………………………………………………………………………….25 REFERENCE…………………………………………………………………………28 | |
| dc.language.iso | en | |
| dc.subject | 轉錄因子 | zh_TW |
| dc.subject | 調控點 | zh_TW |
| dc.subject | 細胞週期素依賴性蛋白激酶 | zh_TW |
| dc.subject | 細胞週期素 | zh_TW |
| dc.subject | 細胞週期 | zh_TW |
| dc.subject | G1 arrest | en |
| dc.subject | CDK | en |
| dc.subject | CDC28 | en |
| dc.subject | G1 cyclin | en |
| dc.subject | Mcm1 | en |
| dc.subject | YPL014w | en |
| dc.title | 酵母菌YPL014W受到Mcm1調控並參與細胞週期之功能分析 | zh_TW |
| dc.title | A novel Mcm1-regulated gene, YPL014W, involved in cell cycle regulation in Saccharomyces cereveciae | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 98-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 李財坤,詹迺立 | |
| dc.subject.keyword | 細胞週期,調控點,細胞週期素依賴性蛋白激酶,細胞週期素,轉錄因子, | zh_TW |
| dc.subject.keyword | CDK,CDC28,G1 cyclin,Mcm1,YPL014w,G1 arrest, | en |
| dc.relation.page | 32 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2010-07-29 | |
| dc.contributor.author-college | 醫學院 | zh_TW |
| dc.contributor.author-dept | 微生物學研究所 | zh_TW |
| 顯示於系所單位： | 微生物學科所 | |
文件中的檔案：
| 檔案 | 大小 | 格式 | |
|---|---|---|---|
| ntu-99-1.pdf 未授權公開取用 | 2.82 MB | Adobe PDF |
系統中的文件，除了特別指名其著作權條款之外，均受到著作權保護，並且保留所有的權利。