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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 嚴震東(Chen-Tung Yen) | |
dc.contributor.author | Yu-Fei Chu | en |
dc.contributor.author | 朱于飛 | zh_TW |
dc.date.accessioned | 2021-06-15T04:58:07Z | - |
dc.date.available | 2012-09-01 | |
dc.date.copyright | 2011-08-22 | |
dc.date.issued | 2011 | |
dc.date.submitted | 2011-08-17 | |
dc.identifier.citation | Abel EL. (1993) Physiological correlates of the forced swim test in rats. Physiol Behav. 54:309-317.
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dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/46208 | - |
dc.description.abstract | 早期的感覺發展需要經過一段特定的早期發育時間,使其相關的神經系統網路發育成熟,稱為發育關鍵期。在大鼠的生物模式中,我們設計了實驗,將新生大鼠的觸鬚剪去,在發育關鍵期中,剝奪其觸覺輸入,之後待其成長至性成熟之年紀,以動物行為及生物化學的方法來探討此一處理對於大鼠之影響。我們發現大鼠在經過早期的鬍鬚剪除後,其觸覺功能在成年後仍受影響。在動物行為實驗中證實,經歷過早期鬍鬚剪除的大鼠,觸覺功能較控制組之大鼠差,在新環境中探索的行為也增加。我們利用組織化學染色,發現其大腦體感覺皮質接收外界訊息之中樞結構層雖並未有形態學上之顯著變化,但經過早期鬍鬚剪除的大鼠,其神經元樹突結構之複雜程度較控制組大鼠簡單,其上樹突棘之密度也較控制組大鼠減少。針對神經元活動的標記分子c-Fos,進行免疫組織染色發現,經歷過早期鬍鬚剪除的大鼠,其大腦皮質對應至鬍鬚之體感覺區內,c-Fos神經元表現數量低於控制組,暗示著體感覺區的神經元活動在皮質分層之間分布的改變,以及相對較低的神經活動程度。我們的實驗證實,早期的鬍鬚剪除,如此看似細微且短期的操作,仍會對大鼠的中樞神經相關功能造成長期的影響。 | zh_TW |
dc.description.abstract | Early sensory experience plays essential roles in development of sensory systems. For example, early surgical manipulations of whiskers in rodents lead to altered neural activity and behaviors later in life. However, the surgical procedures damage the sensory pathway; it is impossible to examine the consequence if recovery of the sensory pathway is unfeasible. To address this issue, we performed a neonatal whisker trimming (WC0-3) paradigm, a non-invasive procedure, from the day of birth (P0) to postnatal day (P) 3, and examined the behavioral performances in their adult life. With fully regrown whiskers, the WC0-3 rats exhibited shorter maximal crossable distance than the controls in the gap-crossing task, suggesting the defect in their whisker-specific tactile function, although the barrel pattern in the somatosensory cortex was not significantly changed. The spiny stellate neurons in layer IV were found to be possessed with decreased complexity of dendritic arbor and spine density. After exploration in a novel environment, the expression of activity-dependent early immediate early gene, c-fos, was increased dramatically in the somatosensory cortex. However, in the WC0-3 rats, the number of c-fos positive cells was much less than that in the control rats, especially in the upper and lower cortical layers, indicating the fault in transducing sensory-related neural activity between cortical layers in the WC0-3 rats. With defect in the somatosensory system, the WC0-3 rats exhibited higher explorative activity in an open field. Our results demonstrated that early tactile deprivation disturbs the brain development and leaves long-lasting functional deficits in the nervous system. | en |
dc.description.provenance | Made available in DSpace on 2021-06-15T04:58:07Z (GMT). No. of bitstreams: 1 ntu-100-R97b41034-1.pdf: 2787707 bytes, checksum: f7a94fd09e1a3da9aa1629f2250912bd (MD5) Previous issue date: 2011 | en |
dc.description.tableofcontents | CONTENTS
中文摘要.........................................................................................................................i Abstract.......................................................................................................................ii Contents.........................................................................................................................iv List of Table..................................................................................................................vi List of Figures...............................................................................................................vi List of Abbreviations…………………………………………………………………vii Introduction……………………………………………………………………………1 I. The somatosensory system of rats……………………………………………1 II. Critical period for the development of whisker-to-barrel system…………...2 III. Experimental design………………………………………………………..4 Materials and Methods...............................................................................................6 I. Subjects............................................................................................................6 II. Behavioral tests...............................................................................................6 II-1. Gap-crossing........................................................................................7 II-2. Open field............................................................................................7 II-3. Elevated plus maze (EPM) .................................................................8 II-4. Forced swim........................................................................................8 II-5. Enriched Environment (EE)...................................................................9 III. Histology......................................................................................................10 III-1. Cytochrome oxidase (CO) stain..................................................10 III-2. Golgi-Cox impregnation...................................................................11 III-3. Immunohistochemistry (IHC) ..........................................................12 III-4. Nissl stain.....................................................................................14 IV. Statistical analyses................................................................................15 Results......................................................................................................................16 I. Behavioral tests...............................................................................................16 I-1. Gap-crossing........................................................................................16 I-2. Open field............................................................................................16 I-3. Elevated plus maze (EPM)..................................................................17 I-4. Forced swim........................................................................................17 II. Morphological analysis.................................................................................18 II-1. Cytochrome oxidase (CO) stain..........................................................18 II-2. Dendrites..............................................................................................18 II-3. Dendritic spines.................................................................................19 III. c-Fos expression..........................................................................................20 Discussion...............................................................................................................22 I. Behavioral aspect...........................................................................................22 I-1. Gap-crossing.......................................................................................22 I-2. Open field..........................................................................................22 I-3. Elevated plus maze..............................................................................23 I-4. Forced swim........................................................................................24 II. Histological aspect........................................................................................24 II-1. Cytochrome oxidase (CO) stain...........................................................24 II-2. Golgi-Cox impregnation and morphometric analysis........................25 II-3. c-Fos expression.................................................................................29 Conclusion...............................................................................................................32 Reference...............................................................................................................33 | |
dc.language.iso | en | |
dc.title | 早期鬍鬚剪除對成年大鼠行為、神經元結構及神經活動之 影響 | zh_TW |
dc.title | Neonatal Whisker Trimming Alters Behavior, Neuronal Structure, and Neural Activity of Adult Rats | en |
dc.type | Thesis | |
dc.date.schoolyear | 99-2 | |
dc.description.degree | 碩士 | |
dc.contributor.coadvisor | 李立仁(Li-Jen Lee) | |
dc.contributor.oralexamcommittee | 賴文崧(Wen-Sung Lai),蔡孟利(Meng-li Tsai) | |
dc.subject.keyword | 大鼠,鬍鬚,觸覺,行為,神經元,形態學,c-Fos,神經元活動, | zh_TW |
dc.subject.keyword | Rat,whisker,tactile sensory,behavior,neuron,morphology,c-Fos,neural activity, | en |
dc.relation.page | 58 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2011-08-18 | |
dc.contributor.author-college | 生命科學院 | zh_TW |
dc.contributor.author-dept | 動物學研究所 | zh_TW |
顯示於系所單位: | 動物學研究所 |
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