請用此 Handle URI 來引用此文件：
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/46206完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 管永恕(Yung-Shu Kuan) | |
| dc.contributor.author | Wei-Han Lang | en |
| dc.contributor.author | 郎偉涵 | zh_TW |
| dc.date.accessioned | 2021-06-15T04:58:02Z | - |
| dc.date.available | 2013-08-02 | |
| dc.date.copyright | 2010-08-02 | |
| dc.date.issued | 2010 | |
| dc.date.submitted | 2010-07-28 | |
| dc.identifier.citation | Reference
Arimura, N., Inagaki, N., Chihara, K., Menager, C., Nakamura, N., Amano, M., Iwamatsu, A., Goshima, Y., and Kaibuchi, K. 2000. Phosphorylation of collapsin response mediator protein-2 by Rho-kinase. Evidence for two separate signaling pathways for growth cone collapse. J Biol Chem 275(31): 23973-23980. Arimura, N., Menager, C., Kawano, Y., Yoshimura, T., Kawabata, S., Hattori, A., Fukata, Y., Amano, M., Goshima, Y., Inagaki, M. et al. 2005. Phosphorylation by Rho kinase regulates CRMP-2 activity in growth cones. Mol Cell Biol 25(22): 9973-9984. Bagnard, D., Thomasset, N., Lohrum, M., Puschel, A.W., and Bolz, J. 2000. Spatial distributions of guidance molecules regulate chemorepulsion and chemoattraction of growth cones. J Neurosci 20(3): 1030-1035. Barnekow, A., Thyrock, A., and Kessler, D. 2009. Chapter 5: rab proteins and their interaction partners. Int Rev Cell Mol Biol 274: 235-274. Bianco, I.H., Carl, M., Russell, C., Clarke, J.D., and Wilson, S.W. 2008. Brain asymmetry is encoded at the level of axon terminal morphology. Neural Dev 3: 9. Bianco, I.H. and Wilson, S.W. 2009. The habenular nuclei: a conserved asymmetric relay station in the vertebrate brain. Philos Trans R Soc Lond B Biol Sci 364(1519): 1005-1020. Buel, G.R., Rush, J., and Ballif, B.A. Fyn promotes phosphorylation of collapsin response mediator protein 1 at tyrosine 504, a novel, isoform-specific regulatory site. J Cell Biochem. Cerda, G.A., Thomas, J.E., Allende, M.L., Karlstrom, R.O., and Palma, V. 2006. Electroporation of DNA, RNA, and morpholinos into zebrafish embryos. Methods 39(3): 207-211. Chae, Y.C., Lee, S., Heo, K., Ha, S.H., Jung, Y., Kim, J.H., Ihara, Y., Suh, P.G., and Ryu, S.H. 2009. Collapsin response mediator protein-2 regulates neurite formation by modulating tubulin GTPase activity. Cell Signal 21(12): 1818-1826. Ekstrom, P. and Ebbesson, S.O. 1988. The left habenular nucleus contains a discrete serotonin-immunoreactive subnucleus in the coho salmon (Oncorhynchus kisutch). Neurosci Lett 91(2): 121-125. Fukata, Y., Itoh, T.J., Kimura, T., Menager, C., Nishimura, T., Shiromizu, T., Watanabe, H., Inagaki, N., Iwamatsu, A., Hotani, H. et al. 2002. CRMP-2 binds to tubulin heterodimers to promote microtubule assembly. Nat Cell Biol 4(8): 583-591. Gamse, J.T., Kuan, Y.S., Macurak, M., Brosamle, C., Thisse, B., Thisse, C., and Halpern, M.E. 2005. Directional asymmetry of the zebrafish epithalamus guides dorsoventral innervation of the midbrain target. Development 132(21): 4869-4881. Gogel, S., Lange, S., Leung, K.Y., Greene, N.D., and Ferretti, P. Post-translational regulation of Crmp in developing and regenerating chick spinal cord. Dev Neurobiol 70(6): 456-471. Goshima, Y., Nakamura, F., Strittmatter, P., and Strittmatter, S.M. 1995. Collapsin-induced growth cone collapse mediated by an intracellular protein related to UNC-33. Nature 376(6540): 509-514. Haas, K., Sin, W.C., Javaherian, A., Li, Z., and Cline, H.T. 2001. Single-cell electroporation for gene transfer in vivo. Neuron 29(3): 583-591. He, Z. and Tessier-Lavigne, M. 1997. Neuropilin is a receptor for the axonal chemorepellent Semaphorin III. Cell 90(4): 739-751. Herkenham, M. and Nauta, W.J. 1977. Afferent connections of the habenular nuclei in the rat. A horseradish peroxidase study, with a note on the fiber-of-passage problem. J Comp Neurol 173(1): 123-146. -. 1979. Efferent connections of the habenular nuclei in the rat. J Comp Neurol 187(1): 19-47. Inagaki, N., Chihara, K., Arimura, N., Menager, C., Kawano, Y., Matsuo, N., Nishimura, T., Amano, M., and Kaibuchi, K. 2001. CRMP-2 induces axons in cultured hippocampal neurons. Nat Neurosci 4(8): 781-782. Kolodkin, A.L., Levengood, D.V., Rowe, E.G., Tai, Y.T., Giger, R.J., and Ginty, D.D. 1997. Neuropilin is a semaphorin III receptor. Cell 90(4): 753-762. Kuan, Y.S., Yu, H.H., Moens, C.B., and Halpern, M.E. 2007. Neuropilin asymmetry mediates a left-right difference in habenular connectivity. Development 134(5): 857-865. Luo, Y., Raible, D., and Raper, J.A. 1993. Collapsin: a protein in brain that induces the collapse and paralysis of neuronal growth cones. Cell 75(2): 217-227. Majava, V., Loytynoja, N., Chen, W.Q., Lubec, G., and Kursula, P. 2008. Crystal and solution structure, stability and post-translational modifications of collapsin response mediator protein 2. FEBS J 275(18): 4583-4596. Martinez, O., Antony, C., Pehau-Arnaudet, G., Berger, E.G., Salamero, J., and Goud, B. 1997. GTP-bound forms of rab6 induce the redistribution of Golgi proteins into the endoplasmic reticulum. Proc Natl Acad Sci U S A 94(5): 1828-1833. Martinez, O., Schmidt, A., Salamero, J., Hoflack, B., Roa, M., and Goud, B. 1994. The small GTP-binding protein rab6 functions in intra-Golgi transport. J Cell Biol 127(6 Pt 1): 1575-1588. Matanis, T., Akhmanova, A., Wulf, P., Del Nery, E., Weide, T., Stepanova, T., Galjart, N., Grosveld, F., Goud, B., De Zeeuw, C.I. et al. 2002. Bicaudal-D regulates COPI-independent Golgi-ER transport by recruiting the dynein-dynactin motor complex. Nat Cell Biol 4(12): 986-992. Merlo, G.R., Zerega, B., Paleari, L., Trombino, S., Mantero, S., and Levi, G. 2000. Multiple functions of Dlx genes. Int J Dev Biol 44(6): 619-626. Morley, B.J. 1986. The interpeduncular nucleus. Int Rev Neurobiol 28: 157-182. Murdoch, C. 2000. CXCR4: chemokine receptor extraordinaire. Immunol Rev 177: 175-184. Nakamura, F., Kalb, R.G., and Strittmatter, S.M. 2000. Molecular basis of semaphorin-mediated axon guidance. J Neurobiol 44(2): 219-229. Opdam, F.J., Echard, A., Croes, H.J., van den Hurk, J.A., van de Vorstenbosch, R.A., Ginsel, L.A., Goud, B., and Fransen, J.A. 2000. The small GTPase Rab6B, a novel Rab6 subfamily member, is cell-type specifically expressed and localised to the Golgi apparatus. J Cell Sci 113 ( Pt 15): 2725-2735. Ostermeier, C. and Brunger, A.T. 1999. Structural basis of Rab effector specificity: crystal structure of the small G protein Rab3A complexed with the effector domain of rabphilin-3A. Cell 96(3): 363-374. Parinov, S., Kondrichin, I., Korzh, V., and Emelyanov, A. 2004. Tol2 transposon-mediated enhancer trap to identify developmentally regulated zebrafish genes in vivo. Dev Dyn 231(2): 449-459. Pereira-Leal, J.B. and Seabra, M.C. 2001. Evolution of the Rab family of small GTP-binding proteins. J Mol Biol 313(4): 889-901. Perlin, J.R. and Talbot, W.S. 2007. Signals on the move: chemokine receptors and organogenesis in zebrafish. Sci STKE 2007(400): pe45. Rohm, B., Ottemeyer, A., Lohrum, M., and Puschel, A.W. 2000. Plexin/neuropilin complexes mediate repulsion by the axonal guidance signal semaphorin 3A. Mech Dev 93(1-2): 95-104. Rossignol, M., Beggs, A.H., Pierce, E.A., and Klagsbrun, M. 1999. Human neuropilin-1 and neuropilin-2 map to 10p12 and 2q34, respectively. Genomics 57(3): 459-460. Rosslenbroich, V., Dai, L., Baader, S.L., Noegel, A.A., Gieselmann, V., and Kappler, J. 2005. Collapsin response mediator protein-4 regulates F-actin bundling. Exp Cell Res 310(2): 434-444. Schmidt, E.F. and Strittmatter, S.M. 2007. The CRMP family of proteins and their role in Sema3A signaling. Adv Exp Med Biol 600: 1-11. Schweitzer, J., Becker, C.G., Schachner, M., and Becker, T. 2005. Expression of collapsin response mediator proteins in the nervous system of embryonic zebrafish. Gene Expr Patterns 5(6): 809-816. Stenmark, P., Ogg, D., Flodin, S., Flores, A., Kotenyova, T., Nyman, T., Nordlund, P., and Kursula, P. 2007. The structure of human collapsin response mediator protein 2, a regulator of axonal growth. J Neurochem 101(4): 906-917. Swift, S., Xu, J., Trivedi, V., Austin, K.M., Tressel, S.L., Zhang, L., Covic, L., and Kuliopulos, A. A novel protease-activated receptor-1 interactor, Bicaudal D1, regulates G protein signaling and internalization. J Biol Chem 285(15): 11402-11410. Takahashi, T., Fournier, A., Nakamura, F., Wang, L.H., Murakami, Y., Kalb, R.G., Fujisawa, H., and Strittmatter, S.M. 1999. Plexin-neuropilin-1 complexes form functional semaphorin-3A receptors. Cell 99(1): 59-69. Uchida, Y., Ohshima, T., Yamashita, N., Ogawara, M., Sasaki, Y., Nakamura, F., and Goshima, Y. 2009. Semaphorin3A signaling mediated by Fyn-dependent tyrosine phosphorylation of collapsin response mediator protein 2 at tyrosine 32. J Biol Chem 284(40): 27393-27401. Wang, L.H. and Strittmatter, S.M. 1997. Brain CRMP forms heterotetramers similar to liver dihydropyrimidinase. J Neurochem 69(6): 2261-2269. Wanschers, B.F., van de Vorstenbosch, R., Schlager, M.A., Splinter, D., Akhmanova, A., Hoogenraad, C.C., Wieringa, B., and Fransen, J.A. 2007. A role for the Rab6B Bicaudal-D1 interaction in retrograde transport in neuronal cells. Exp Cell Res 313(16): 3408-3420. Yazdani, U. and Terman, J.R. 2006. The semaphorins. Genome Biol 7(3): 211. Yoshimura, T., Arimura, N., Kawano, Y., Kawabata, S., Wang, S., and Kaibuchi, K. 2006. Ras regulates neuronal polarity via the PI3-kinase/Akt/GSK-3beta/CRMP-2 pathway. Biochem Biophys Res Commun 340(1): 62-68. Yu, H.H., Houart, C., and Moens, C.B. 2004. Cloning and embryonic expression of zebrafish neuropilin genes. Gene Expr Patterns 4(4): 371-378. Zerial, M. and McBride, H. 2001. Rab proteins as membrane organizers. Nat Rev Mol Cell Biol 2(2): 107-117. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/46206 | - |
| dc.description.abstract | 雙邊的疆核(HA)及其聯接到interpeduncular核(IPN)的結構是在脊椎動物腦中最保守的神經迴路，其扮演了許多重要的角色如調控認知,厭惡反應及回饋反應。之前的研究指出在斑馬魚胚胎發育時，分泌性的指引線索Semaphorin 3D(Sema3D)會吸引位於左邊表現Neuropilin 1A(Nrp1A)的神經元軸突延伸到中腦IPN的背面區塊。然而如何去闡釋由Sema3D所引起疆核神經元軸突吸引的下游分子機制仍不清楚。利用收尋法，我已經確定collapsin response mediator proteins (CRMPs) 1, 4, 5a,及Ras-related in brain 6b like (Rab6bl)在斑馬魚受精後72及96小時胚胎中的疆核表現。尤其，Rab6bl和Nrp1A的轉錄產物會共位於斑馬魚胚胎左邊疆核在受精後HA-IPN 迴路形成的72到96小時。利用顯微注射過量表現野生型(WT)及負顯性型(DN)的Rab6bl在1到2個細胞時期並沒有造成可以被偵測到的分子缺陷。這顯示Rab6bl在早期胚胎發育中可能沒有顯著的功能。這個結果跟我在受精後48小時前所觀察到沒有明顯的表現量一致。為了特別去破壞Rab6bl受精後72到96小時在左邊疆核的功能，我採用了電穿孔的技術並且在目前的裝備(Axoporator)設立及實驗條件下，利用100伏特(V)，200赫茲(Hz)及2毫秒寬度的方形波重複七次電刺激可以達到最高的成功率。 | zh_TW |
| dc.description.abstract | The bilateral habenula nuclei (HA) and their connections to the interpeduncular nucleus (IPN) structure one of the most highly conserved circuits in vertebrate brains that plays many important roles such as regulating cognition, aversive response and reward processing. Prior study showed that during zebrafish embryonic development, secreted guidance cues Semaphorin 3D (Sema3D) attract subset of Neuropilin 1A (Nrp1a) positive neuronal axons from the left HA to their targets in the dorsal IPN domain in the midbrain. However, the downstream molecular mechanism of how HA neurons interpret this Sema3D-mediated axonal attraction event still remains elusive. Utilizing candidate gene search approaches, I have identified that collapsin response mediator proteins (CRMPs) 1, 4, 5a, and Ras-related in brain 6b like (Rab6bl) are expressed in HA in zebrafish embryos at 72 and 96 hours post fertilization (hpf). Particularly, the Rab6bl and the Nrp1a transcrips are co-localized within the left HA during the formation of HA-IPN connections between 72-96 hpf. Over-expressions of wildtype (WT) and dominant-negative forms of Rab6bl (DN-Rab6bl) by injecting in vitro transcribed mRNAs into 1-2 cell-stage embryos caused no detectable molecular defect suggesting that Rab6bl has no obvious function during early embryonic development. This result is consistent with my observation that there is no detectable expression of rab6bl before 48 hpf. In order to specifically perturb the function of Rab6bl in left HA during 72-96 hpf, I have adopted the electroporation technique, and under my current equipment set-up and experimental condition, the use of 100V, 200Hz and 2.0mS squire wave width for 7 times exhibited the highest successful rate with the Axonporator 800 apparatus. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-15T04:58:02Z (GMT). No. of bitstreams: 1 ntu-99-R96b46008-1.pdf: 8512295 bytes, checksum: f5aafbc261ab9b249309faa2ee929420 (MD5) Previous issue date: 2010 | en |
| dc.description.tableofcontents | Table of content
中文摘要-------------------------------------------------------------------------------------------vi Abstract---------------------------------------------------------------------------------------------vii I. Introduction --------------------------------------------------------------------------------------1 1. Structure and function of habenula-interpeduncular nucleus circuit---------------------2 2. Roles of Neuropilins, Semaphroins and Plexins in the axon guidance------------------2 3. Collapsin Response Mediator Proteins (CRMPs)------------------------------------------4 4. Ras realted in Brain (RABs)------------------------------------------------------------------5 I. Motivation---------------------------------------------------------------------------------------7 II. Material and Methods-------------------------------------------------------------------------9 III. Results---------------------------------------------------------------------------------------- 24 IV. Discussion------------------------------------------------------------------------------------32 VI. Figure----------------------------------------------------------------------------------------39 VII. Reference------------------------------------------------------------------------------------49 VIII. Appendix------------------------------------------------------------------------------------53 | |
| dc.language.iso | en | |
| dc.title | 剖析Semaphorin 3D-Neuropilin 1A引導疆核軸突目標辨認的分子機制 | zh_TW |
| dc.title | Dissecting the Molecular Mechanism of Semaphorin3D- Neuropilin 1A Guided Habenula Axonal
Target Recognition | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 98-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 張震東(Geen-Dong Chang),劉福清(Fu-Chin Liu),黃聲蘋(Sheng-Ping Hwang) | |
| dc.subject.keyword | HA-IPN迴路,Neuropilin1A-Semaphorin3D 訊息傳遞,CRMP,Rab6bl,電穿孔, | zh_TW |
| dc.subject.keyword | habenula-interpedunclur nucleus circuit,Neuropilin1A-Semaphorin3D signaling,CRMPs,Rab6bl,electroporation, | en |
| dc.relation.page | 54 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2010-07-29 | |
| dc.contributor.author-college | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 生化科學研究所 | zh_TW |
| 顯示於系所單位： | 生化科學研究所 | |
文件中的檔案：
| 檔案 | 大小 | 格式 | |
|---|---|---|---|
| ntu-99-1.pdf 目前未授權公開取用 | 8.31 MB | Adobe PDF |
系統中的文件，除了特別指名其著作權條款之外，均受到著作權保護，並且保留所有的權利。