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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 張震東 | |
| dc.contributor.author | Yu-Jung Lee | en |
| dc.contributor.author | 李昱蓉 | zh_TW |
| dc.date.accessioned | 2021-06-15T04:57:16Z | - |
| dc.date.available | 2011-07-30 | |
| dc.date.copyright | 2010-07-30 | |
| dc.date.issued | 2010 | |
| dc.date.submitted | 2010-07-29 | |
| dc.identifier.citation | [1] J.-Y. Wang, R.A. Casero, SpringerLink (Online service), Polyamine Cell Signaling Physiology, Pharmacology, and Cancer Research, Humana Press Inc., Totowa, NJ, 2006, pp. xiv, 490 p.
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/46188 | - |
| dc.description.abstract | 組蛋白的轉譯後修飾，與細胞生長和基因表現等多種重要調控功能有關。在由HeLa細胞中純化的組蛋白上，可被精胺抗體偵測到可能是共價修飾的訊號；而多胺類對於細胞生長相關的生理功能亦具有調控地位。與以大腸桿菌表現的重組組蛋白，以及其他由細胞純化、具有與組蛋白類似性質的蛋白質進行比較，我們認為HeLa細胞的組蛋白上具有精胺的共價修飾，並且以TAU/SDS二維電泳以及組蛋白抗體確認在H3、H2B和H4上皆發現此訊號。而轉穀醯胺酶是真核細胞中催化多胺類共價修飾最主要的酵素，我們以實驗確認轉穀醯胺酶能表現於HeLa細胞核中，且組蛋白為轉穀醯胺酶的穀氨醯基受質(acyl donor substrate)，並進一步對對HeLa細胞純化組蛋白以及重組組蛋白進行in vitro轉穀醯胺酶催化精胺修飾的實驗，發現在重組的H2B上有發現精胺修飾的狀況，但在in vivo加入轉穀醯胺酶抑制劑cystamine時卻沒有看到修飾程度有明顯的變化。另外，以lovastatin使細胞週期同步化後每四小時分別測定組蛋白的精胺修飾程度，發現在G2/M期的細胞會有較多的精胺修飾。因此，組蛋白的精胺修飾可能存在與某些細胞週期相關的變化有關的生理意義。 | zh_TW |
| dc.description.abstract | The post-translational modifications of histones are relevant to many important cellular regulation mechanisms essential for cell proliferation and gene expression. In the histones extracted from HeLa cells, incorporation of spermine, presumably by covalent modification, was detected. Interestingly, these polyamines also play a key role in the regulation of cell proliferation.
From our results, we showed that spermine is covalently attached to extracted histones derived from HeLa cells. Comparing the western blotting results of extracted histones with that of recombinant human core histones and other proteins which have similar features to histones, we detected positive anti-spermine signals in histones H3, H2B and H4. This was further confirmed in our TAU/SDS 2D PAGE data. Since tissue transglutaminase (TG2) is the main enzyme catalyzing the protein-polyamine incorporations, we confirmed that TG2 expressed in the nuclei of HeLa cells, and that histones were the in vitro acyl donor substrates of TG2. However, from the in vitro transamidation assay, we found that only H2B could be modified. Treatment of HeLa cells with cystamine, a TG2 inhibitor, did not alter the spermine-conjugation levels in core histones. Furthermore, we synchronized the HeLa cells by lovastatin treatments and harvested the cells every four hours after release of lovastatin inhibition to determine the levels of spermine incorporation. We found that cells in the G2/M phase of the cell cycle had higher levels of spermine incorporation compared to those in the other stages. These results may imply a functional role of the modification of histones by spermine in cell cycle regulations. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-15T04:57:16Z (GMT). No. of bitstreams: 1 ntu-99-R97b46004-1.pdf: 4446653 bytes, checksum: 09cab6416cbebd4009ce1bbb52696973 (MD5) Previous issue date: 2010 | en |
| dc.description.tableofcontents | 口試委員會審定書
誌謝 i 中文摘要 iii 英文摘要 iv 縮寫表 v 第一章 緒論 1 第一節 多胺類生合成與生理功能 1 第二節 多胺類結合與修飾作用 2 第三節 多胺類對染色體與核酸之影響 2 第四節 多胺類對細胞生長和細胞週期之影響 3 第五節 組蛋白之生化特性、轉譯後修飾與生理功能 4 第六節 轉穀醯胺酶對組蛋白之調控 5 第七節 研究動機與目的 7 第二章 實驗材料與方法 8 第一節 實驗材料 8 第二節 細胞培養 8 第三節 人類細胞內組蛋白之純化 8 第四節 重組人類組蛋白之取得 8 第一項 引子設計 (Primer Designing) 8 第二項 基因組去氧核醣核酸(Genomic DNA)之萃取 9 第三項 聚合酶連鎖反應 (Polymerase Chain Reaction) 9 第四項 基因轉殖 (Cloning) 10 第五項 重組蛋白質表現與純化 10 第五節 蛋白質實驗方法 11 第一項 SDS聚丙烯醯胺凝膠電泳 (SDS Poly-acrylamide Gel Electrophoresis, SDS-PAGE) 11 第二項 Triton酸性尿素聚丙烯醯胺凝膠電泳(Triton-Acid-Urea Poly-acrylamide Gel Electrophoresis, TAU PAGE)與TAU/SDS二維式電泳 12 第三項 蛋白質電泳膠片染色與銀染 13 第四項 BCA蛋白質定量 13 第五項 電泳轉移 (Electrophoretic Transfer) 13 第六項 西方轉漬法 (Western Blotting) 14 第七項 細胞外轉穀醯胺酶之催化反應 (in vitro Tissue Transglutaminase 14 Catalysis) 14 第六節 細胞學實驗方法 14 第一項 繼代培養 14 第二項 細胞核與細胞質之分離 15 第三項 細胞均質液(whole cell lysate)製備 15 第四項 細胞週期之藥物控制與偵測 15 第五項 抑制藥物之處理 16 第六項 免疫螢光染色 (Immunofluorescence) 16 第七節 抗體純化 16 第一項 多株抗體純化 16 第三章 實驗結果 17 第一節 由HeLa細胞純化之組蛋白可被精胺專一性抗體偵測到訊號 17 第二節 精胺修飾於細胞純化之其他類似性質蛋白質及重組組蛋白上未被偵測到 17 第三節 轉穀醯胺酶可在細胞核表現且組蛋白為其受質 18 第四節 重組之組蛋白在in vitro情況下受轉穀醯胺酶催化精胺修飾試驗 19 第五節 調控in vivo轉穀醯胺酶活性可能影響細胞內之組蛋白精胺修飾 19 第六節 Lovastatin不適於細胞同步化(cell synchronization) 20 第七節 組蛋白之精胺修飾與細胞週期有關 21 第八節 Doxorubicin不適於討論組蛋白精胺修飾 21 第四章 討論 23 第一節 HeLa細胞純化之組蛋白具有精胺之修飾 23 第二節 組蛋白上的多胺類修飾位置影響其功能 25 第三節 轉穀醯胺酶可能催化組蛋白的多胺類修飾 26 第四節 組蛋白精胺修飾隨細胞週期而變化 28 第五章 結語 30 第六章 實驗結果圖表 31 圖一 HeLa細胞的組蛋白可被偵測到多胺類修飾之訊號 31 圖二 HeLa細胞組蛋白上的多胺類修飾訊號在其他具類似化學性質之蛋白質與重組組蛋白尚皆未發現 33 圖三 In vitro轉穀醯胺酶催化重組組蛋白之精胺修飾 36 圖四 HeLa細胞受cystamine處理後組蛋白修飾程度無明顯改變 37 圖五 以lovastatin處理HeLa細胞對細胞週期影響 38 圖六 組蛋白之精胺修飾程度與細胞週期關係 41 圖七 以doxorubicin處理細胞並偵測精胺修飾程度之差異 43 參考文獻 44 附錄 53 附圖一 三種多胺類putrecine，spermidine和spermine之結構及pKa值 53 附圖二 哺乳類動物之多胺類生合成與代謝途徑 54 附圖三 轉穀醯胺酶催化之轉胺作用機制 55 附圖四 多胺類參與真核細胞生長相關之訊息傳遞 57 附圖五 多胺類參與原核與真核細胞轉譯與細胞生長途徑 58 附圖六 組蛋白八聚體與核小體結構 61 附圖七 組蛋白蛋白質序列 62 附圖八 組蛋白N端之轉錄後修飾 64 附圖九 轉穀醯胺酶催化之組蛋白連結(cross-link) 65 附圖十 人類組蛋白純化方法 66 附圖十一 Lovastatin影響細胞週期之機制 67 附表一 轉穀醯胺酶家族 68 | |
| dc.language.iso | zh-TW | |
| dc.subject | 細胞週期 | zh_TW |
| dc.subject | 組蛋白 | zh_TW |
| dc.subject | 多胺類 | zh_TW |
| dc.subject | 轉穀醯胺酶 | zh_TW |
| dc.subject | 共價修飾 | zh_TW |
| dc.subject | tissue transglutaminase | en |
| dc.subject | cell cyle | en |
| dc.subject | covalent modification | en |
| dc.subject | polyamines | en |
| dc.subject | histones | en |
| dc.title | 轉穀醯胺酶催化之HeLa細胞中組蛋白多胺類修飾 | zh_TW |
| dc.title | Tissue Transglutaminase-induced Histones Polyamination in HeLa Cells | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 98-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 黃銓珍,李明亭,陳宏文,張茂山 | |
| dc.subject.keyword | 組蛋白,多胺類,轉穀醯胺酶,共價修飾,細胞週期, | zh_TW |
| dc.subject.keyword | histones,polyamines,tissue transglutaminase,covalent modification,cell cyle, | en |
| dc.relation.page | 68 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2010-07-29 | |
| dc.contributor.author-college | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 生化科學研究所 | zh_TW |
| 顯示於系所單位： | 生化科學研究所 | |
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