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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 潘子明 | |
dc.contributor.author | Wei-Hong Jheng | en |
dc.contributor.author | 鄭偉宏 | zh_TW |
dc.date.accessioned | 2021-06-15T04:57:10Z | - |
dc.date.available | 2014-08-26 | |
dc.date.copyright | 2011-08-26 | |
dc.date.issued | 2011 | |
dc.date.submitted | 2011-08-17 | |
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李俊霖。2003。提高紅麴中 monacolin K、GABA 與降低 citrinin 生成量之研究。國立台灣大學農業化學研究所碩士論文。台北,臺灣,中華民國。 林讚峰。2009。紅麴在現代生物科技上的應用。健康世界。台北,臺灣,中華民國。283: 21-26 侯安琪。1979。利用氣舉式發酵槽培養紅麴菌以生產色素之研究。國立台灣大學化學工程學研究所碩士論文。台北,臺灣,中華民國。 洪璽凱。2007。紅麴山藥之最適發酵生產與預防粥狀動脈硬化之體內評估。國立台灣大學微生物與生化學研究所碩士論文。台北,臺灣,中華民國。 楊欣儀、簡相堂。2005。紅麴及其衍生產品之市場調查與分析。財團法人食品工業發展研究所。新竹,臺灣,中華民國。 蘇遠志、陳文亮、方鴻源、翁浩慶與王文祥。1970。紅麴菌 (Monascus anka) 之菌學研究。中國農業化學會誌。21: 63-71。 蘇遠志、黃健雄。1976。紅麴色素之生產研究。中國農業化學會誌。14: 45-58。 蘇遠志。2001。神奇的紅麴。元氣齋出版社。台北,臺灣,中華民國。 Akihisa, T., Tokuda, H., Ukiya, M., Kiyota, A., Yasukawa, K., Sakamoto, N., Kimura, Y., Suzuki, T., Takayasu, J., and Nishino, H. 2005. Anti-tumor-initiating effects of monascin, an azaphilonoid pigment from the extract of Monascus pilosus fermented rice (red-mold rice). Chem Biodivers 2: 1305-1309. Alberts, A. 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dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/46186 | - |
dc.description.abstract | 近幾年來因消費者健康意識抬頭,對傳統合成色素安全性的疑慮,加上政府衛生管理日趨嚴格的情況下,全球的天然色素市場已有不斷的增加的趨勢。紅麴色素為紅麴次級代謝物之一,屬於一群具有 azaphilone 結構的聚縮酮 (polyketides) 天然物。近年來有文獻指出紅麴之黃色素 monascin 與 ankaflavin 具有良好的生理活性功能,例如抗發炎、抑制癌細胞生長等,為極具保健功效潛力的天然化合物。本研究分別利用液態與固態兩種培養系統,探討起始 pH 值、溫度、不同碳氮源種類、乙醇添加、水添加與培養時間等不同培養條件,對 Monascus purpureus NTU 568 黃色素生產之影響,再以反應曲面法 (response surface methodology, RSM) 找出 monascin 與 ankaflavin 最適化生產的條件組合。液態培養結果顯示培養時間八天,澱粉濃度 3.8%、起始 pH 5.2 及乙醇添加 0.5% 時,monascin由原先 9.8 mg/g 提升至 17.4 mg/g、ankaflavin 由 4.4 mg/g 提升至 6.5 mg/g,而 citrinin 則從 3.3 ppm 稍微提升至為 4.4 ppm。以山藥作為固態發酵培養基質時,當培養溫度為 35oC、加水量 200 mL、乙醇添加 0.5% 時,monascin由原先 3.4 mg/g 提升至 9.4 mg/g、ankaflavin 由 1.2 mg/g 提升至 3.8 mg/g,而 citrinin 則從 5.4 ppm 降為 2.9 ppm。將反應曲面法所得之結果以攪拌式發酵槽與氣舉式發酵槽進行放大培養,攪拌式發酵槽所得之 monascin 與 ankaflavin 產量分別為28.1 mg/g 及 15.8 mg/g,citrinin 產量為 3.3 mg/kg;氣舉式發酵槽所得之 monascin 與 ankaflavin 產量分別為 25.9 mg/g 及 14.3 mg/g,citrinin 產量為 4.8 mg/kg。 | zh_TW |
dc.description.abstract | The market of the natural products on an international level is in a phase of extraordinary expansion. The pigments of Monascus spp. are secondary metabolites belong to the group of polyketides. Monascus pigments composed of yellow, orange and red pigments which belonged to an azaphilone skeleton, and have been reported to possess anti-inflammantory and anticancer activities. In this study, Monascus purpureus NTU 568 was fermented under solid and submerged conditions, and culture conditions (cultural time, initial pH value, carbon source, nitrogen source, cultural temperature and ethanol addition) were selected to investigate the effect on monascin and ankaflavin production. Furthermore, response surface methodology (RSM) was applied in order to seek the optimal culture conditions. Culture conditions were as following: with 3.8% carbon source concentration, initial pH value were 5.2 and adding 0.5% ethanol, after 8-days cultivation the production of monascin and ankaflavin would be increase from 9.8 mg/g to 17.4 mg/g and 4.5 to 6.6 mg/g respectively, citrinin would be increase from 3.3 ppm to 4.4 ppm in submerged fermentation. In addition, while dioscorea was used as the solid culture substrate, and the optimal culture conditions were as following: cultural temperature 35oC, 200 mL water and 0.5% ethanol were added, after 8-days cultivation, the production of monascin and ankaflavin would be increase from 3.4 mg/g to 9.4 mg/g and 1.3 to 3.4 mg/g respectively, citrinin would be decreased from 5.4 ppm to 2.9 ppm. Afterward, the optimal cultivation conditions were carried out using stirred tank fermentor and airlift fermentor. The production of monascin and ankaflavin were 28.1 mg/g and 15.8 mg/g respectively, citrinin concentration was 3.3 mg/kg in strried tank fermentor; on the other hand, the production of monascin and ankaflavin were 25.9 mg/g and 14.3 mg/g respectively, citrinin concentration was 4.8 mg/kg in airlift fermentor. | en |
dc.description.provenance | Made available in DSpace on 2021-06-15T04:57:10Z (GMT). No. of bitstreams: 1 ntu-100-R97b47115-1.pdf: 8676099 bytes, checksum: ccfa1649fd763d66d8f8c70c2f525339 (MD5) Previous issue date: 2011 | en |
dc.description.tableofcontents | 縮寫表 I
摘要 II Abstract III 目錄 V 圖目錄 VIII 表目錄 X 第一章 前言 1 第二章 文獻回顧 2 第一節 天然色素概述 2 1.1 食用色素使用歷史 2 1.2 天然色素的發展 2 1.2.1 來自微生物之天然色素 3 1.2.2 篩選具有生產色素能力真菌之策略 5 1.3 天然色素市場 8 第二節 紅麴 10 2.1 紅麴之使用歷史與相關記載 10 2.2 紅麴菌簡介與分類 11 2.3 紅麴的製作方法 13 2.3.1 固態發酵 (solid-state fermentation, SSF) 13 2.3.2 液態發酵 (submerged fermentation) 14 2.4 紅麴的次級代謝物 15 2.4.1 膽固醇合成抑制劑- monacolin K 15 2.4.2 γ-胺基丁酸 (γ-aminobutyric acid, GABA) 17 2.4.3 橘黴素 (citrinin) 18 2.4.4 紅麴色素 18 2.5 紅麴黃色素的相關研究 29 第三章 研究動機與目的 31 第四章 材料與方法 33 第一節 菌株培養與保存 33 第二節 儀器 34 第三節 藥品 36 第四節 液態發酵方法與條件 37 第五節 固態發酵方法與條件 38 第六節 紅麴代謝物與色素分析方法 40 第七節 液態發酵培養基最適化試驗 41 第八節 以反應曲面法探討最適培養條件之試驗 42 第五章 結果與討論 48 第一節 紅麴次級代謝物 monascin、ankaflavin 與citrinin 分析方法之調整 48 第二節 最適培養條件之單因子試驗 (液態發酵) 54 2-1 紅麴之生長與代謝物生成曲線 54 2-2 最適碳源探討 57 2-3 最適氮源探討 60 2-4 最適碳源濃度探討 63 2-5 最適培養溫度探討 66 2-6 最適培養 pH 值試驗 69 2-7 額外添加乙醇對次級代謝物之影響 73 2-8 液態發酵之反應曲面法 (response surface methodology, RSM) 試驗 76 第三節 最適培養條件之單因子試驗 (固態發酵) 86 3-1 固態培養基質選擇 86 3-2 最適培養溫度探討 86 3-3乙醇添加之影響 90 3-4 加水量之影響 90 3-5 氮源之影響 90 3-6 固態發酵之反應曲面法試驗 93 第四節 發酵槽放大培養試驗 100 4-1 攪拌式發酵槽 100 4-2 氣舉式發酵槽 102 4-3 發酵槽結果綜合比較 102 第六章 結論 106 參考文獻 109 | |
dc.language.iso | zh-TW | |
dc.title | 紅麴黃色素 monascin 與 ankaflavin 最適生產條件之研究 | zh_TW |
dc.title | Study on the optimal production conditions of Monascus-fermented yellow pigments monascin and ankaflavin | en |
dc.type | Thesis | |
dc.date.schoolyear | 99-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 蘇遠志,黃健雄,鄭建瑋,李俊霖 | |
dc.subject.keyword | 紅麴,monascin,ankaflavin,citrinin,反應曲面法 (RSM), | zh_TW |
dc.subject.keyword | Monascus,monascin,ankaflavin,citrinin,response surface methodology (RSM), | en |
dc.relation.page | 117 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2011-08-18 | |
dc.contributor.author-college | 生命科學院 | zh_TW |
dc.contributor.author-dept | 生化科技學系 | zh_TW |
顯示於系所單位: | 生化科技學系 |
文件中的檔案:
檔案 | 大小 | 格式 | |
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ntu-100-1.pdf 目前未授權公開取用 | 8.47 MB | Adobe PDF |
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。