請用此 Handle URI 來引用此文件：
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/46150完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 胡文聰(Andrew M. Wo) | |
| dc.contributor.author | Chan-Chia Tseng | en |
| dc.contributor.author | 曾展嘉 | zh_TW |
| dc.date.accessioned | 2021-06-15T04:55:42Z | - |
| dc.date.available | 2012-07-30 | |
| dc.date.copyright | 2010-07-30 | |
| dc.date.issued | 2010 | |
| dc.date.submitted | 2010-07-29 | |
| dc.identifier.citation | Reference
1. Di Carlo, D. and L.P. Lee, Dynamic single-cell analysis for quantitative biology. Analytical Chemistry, 2006. 78(23): p. 7918-7925. 2. Harwood, M.M., J.V. Bleecker, P.S. Rabinovitch, and N.J. Dovichi, Cell cycle-dependent characterization of single MCF-7 breast cancer cells by 2-D CE. Electrophoresis, 2007. 28(6): p. 932-937. 3. Neher, E. and B. Sakmann, SINGLE-CHANNEL CURRENTS RECORDED FROM MEMBRANE OF DENERVATED FROG MUSCLE-FIBERS. Nature, 1976. 260(5554): p. 799-802. 4. Tan, S.C.W., W.X. Pan, G. Ma, N. Cai, K.W. Leong, and K. Liao, Viscoelastic behaviour of human mesenchymal stem cells. Bmc Cell Biology, 2008. 9: p. 7. 5. Seo, J., C. Ionescu-Zanetti, J. Diamond, R. Lal, and L.P. Lee, Integrated multiple patch-clamp array chip via lateral cell trapping junctions. Applied Physics Letters, 2004. 84(11): p. 1973-1975. 6. Rettig, J.R. and A. Folch, Large-scale single-cell trapping and imaging using microwell arrays. Analytical Chemistry, 2005. 77(17): p. 5628-5634. 7. Yang, M.S., C.W. Li, and J. Yang, Cell docking and on-chip monitoring of cellular reactions with a controlled concentration gradient on a microfluidic device. Analytical Chemistry, 2002. 74(16): p. 3991-4001. 8. Di Carlo, D., L.Y. Wu, and L.P. Lee, Dynamic single cell culture array. Lab on a Chip, 2006. 6(11): p. 1445-1449. 9. Wu, L.Y., D. Di Carlo, and L.P. Lee, Microfluidic self-assembly of tumor spheroids for anticancer drug discovery. Biomedical Microdevices, 2008. 10(2): p. 197-202. 10. Ashkin, A., J.M. Dziedzic, J.E. Bjorkholm, and S. Chu, OBSERVATION OF A SINGLE-BEAM GRADIENT FORCE OPTICAL TRAP FOR DIELECTRIC PARTICLES. Optics Letters, 1986. 11(5): p. 288-290. 11. Taff, B.M., S.P. Desai, and J. Voldman, Electroactive hydrodynamic weirs for microparticle manipulation and patterning. Applied Physics Letters, 2009. 94(8): p. 3. 12. Inglis, D.W., R. Riehn, R.H. Austin, and J.C. Sturm, Continuous microfluidic immunomagnetic cell separation. Applied Physics Letters, 2004. 85(21): p. 5093-5095. 13. Evander, M., L. Johansson, T. Lilliehorn, J. Piskur, M. Lindvall, S. Johansson, M. Almqvist, T. Laurell, and J. Nilsson, Noninvasive acoustic cell trapping in a microfluidic perfusion system for online bioassays. Analytical Chemistry, 2007. 79(7): p. 2984-2991.. 14. Lutz, B.R., J. Chen, and D.T. Schwartz, Hydrodynamic tweezers: 1. Noncontact trapping of single cells using steady streaming microeddies. Analytical Chemistry, 2006. 78(15): p. 5429-5435. 15. 林建明, ' A microfluidic platform for micromixing, trapping and real-time monitoring of single cells via microvortices',台灣大學博士論文,民國97年. 16. Lin, C.M., Y.S. Lai, H.P. Liu, and A.M. Wo, Microvortices and recirculating flow generated by an oscillatory microplate for microfluidic applications. Applied Physics Letters, 2008. 93(13): p. 3. 16. Lin, C.M., C.C. Tseng, and C.L. Chen, Hydrodynamic Single-cell trapping for Cellular Assays, in Transducers. 2009: Denver, USA. p. 612-615. 17 Lin, C.M., Y.S. Lai, H.P. Liu, C.Y. Chen, and A.M. Wo, Trapping of Bioparticles via Microvortices in a Microfluidic Device for Bioassay Applications. Analytical Chemistry, 2008. 80(23): p. 8937-8945. 18. Nilsson, J., M. Evander, B. Hammarstrom, and T. Laurell, Review of cell and particle trapping in microfluidic systems. Analytica Chimica Acta, 2009. 649(2): p. 141-157. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/46150 | - |
| dc.description.abstract | 對於量測不同細胞的反應，單細胞抓取扮演著相當重要的角色。本篇論文的目的為提供一個可以被利用於單細胞抓取與其相關研究的非接觸式微流體平台，並對此平台與其流場做一系列力學特性的研究。
此平台利用了羅倫茲力驅動一平板做垂直於軸向的往復式運動並產生一區域性低壓區（或稱其為水力井）。利用此區域性低壓區將細胞抓在平板邊緣上方。細胞因流場中非線性的流動所產生的低壓區而懸浮並固定在空間中以抵擋背景流速。而元件的製作則是利用傳統的微影與軟微影製程。直徑五微米到六十微米大小的聚苯乙烯微粒子被用來測試不同大小範圍低壓區的箝制效果。懸浮型的Jurkat細胞則被利用來測試水力井對於細胞的選擇性與其可分離不同尺寸細胞的能力。藉由商用流場模擬軟體的幫助得以描繪出振動平台對於流場流速的變化以及流場中壓力分佈的變化。 結果顯示只需峰間值在三到五伏特的驅動電壓即可達到箝制力的大小在22~96pN的範圍。由因次分析與模擬的結果可知振動平板的振幅為產生較大壓力變化之低壓區的一關鍵參數。本平台可利用適當的條件下可抓取不同大小尺寸細胞。此微流平台提供了單細胞分析一種強而有力箝制單細胞或是多個獨立單細胞的方法。 | zh_TW |
| dc.description.abstract | Single cell trapping for measuring different cellular response is important for a variety of applications including rare cell studies, minimal residual disease, and improved understanding of basic cell biology. The purpose of this thesis is to provide an integrated noninvasive microfluidic platform which can trap single cells for subsequent studies.
The proposed platform utilizes Lorentz force to drive an oscillating microplate generating localized low pressure region, or a hydrodynamic well, in order to trap single cells along the edge of the microplate. Cells are levitated by a time-mean low pressure due to nonlinear flow streaming, fixing cells in a spatial region against background flow. Fabrication of the device involved conventional photolithographic soft lithographic processes, with two masks for the microstructure and one for the microchannel. Different sizes of polystyrene particles were used to verify the wide range of single cell trapping feasible. Jurkat cells in suspended state were used to test the selectivity of hydrodynamic well and the ability to sort different size. Dimensional analysis was used to characterize the physics of the flow filed by commercial simulation software (COMSOL Multiphysics®). Results show the trapping force is in the range of dozens of pico-Newton with only 3 to 5 Vpp of driving voltage. Ability to select different size of cell shows the capability to trap different cell sizes with proper conditions. This microfluidic device provides a robust approach to trap single cells or multiple isolated cells effectively for cellular analysis. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-15T04:55:42Z (GMT). No. of bitstreams: 1 ntu-99-R97543073-1.pdf: 3240357 bytes, checksum: 3cfbad4b21c7e60317bbd779b4c7d58b (MD5) Previous issue date: 2010 | en |
| dc.description.tableofcontents | 口試委員審定書 1
序言與謝辭 2 目錄 3 圖目錄 4 中文摘要 6 Abstract 7 Chapter 1. Introduction 9 Chapter 2. Theory 17 2.1. The Navier-Stokes equations 17 2.2. Hypothesis of pressure difference 18 2.2.1. Dimensional analysis 19 2.3. Simulation of the time-mean flow field 22 2.3.1. Time-mean velocity field 24 2.3.2. Pressure gradient 24 2.4. Device characterization 26 Chapter 3. Material and Method 28 3.1. Fabrication of suspended structure 28 3.2. Fabrication of PDMS microchannel 30 3.3. Device package 32 3.4. Preparation of particles and cells 34 3.5. Fluorescent microscope and dyes 35 3.6. Device operation 35 Chapter 4. Results and Discussion 38 4.1. Pressure difference analysis with numerical result 38 4.2. Trapping force and trapping efficiency 41 4.2.1. Trapping force characterized via single particles 41 4.2.2. Single cell trapping efficiency 46 4.3. Size selectivity of hydrodynamic well 48 4.4. Sorting different size of bio-particle 50 Chapter 5. Conclusions 52 Reference 53 | |
| dc.language.iso | en | |
| dc.subject | 細胞操控 | zh_TW |
| dc.subject | 因次分析 | zh_TW |
| dc.subject | 單細胞抓取 | zh_TW |
| dc.subject | 水力井 | zh_TW |
| dc.subject | dimensionless analysis | en |
| dc.subject | cell manipulation | en |
| dc.subject | noninvasive | en |
| dc.subject | single-cell trapping | en |
| dc.subject | hydrodynamic force | en |
| dc.title | 水力井應用於非接觸式單細胞抓取之微流體元件特性研究 | zh_TW |
| dc.title | Characterization of a Hydrodynamic Well for Non-invasive Trapping of Single Cells in a Microfluidic Device | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 98-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 李雨,李心予 | |
| dc.subject.keyword | 單細胞抓取,細胞操控,水力井,因次分析, | zh_TW |
| dc.subject.keyword | single-cell trapping,noninvasive,cell manipulation,hydrodynamic force,dimensionless analysis, | en |
| dc.relation.page | 55 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2010-07-30 | |
| dc.contributor.author-college | 工學院 | zh_TW |
| dc.contributor.author-dept | 應用力學研究所 | zh_TW |
| 顯示於系所單位： | 應用力學研究所 | |
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