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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 沈弘俊(Horn-Jiunn Sheen) | |
| dc.contributor.author | Chung-An Lin | en |
| dc.contributor.author | 林重安 | zh_TW |
| dc.date.accessioned | 2021-06-15T04:52:54Z | - |
| dc.date.available | 2015-08-02 | |
| dc.date.copyright | 2010-08-02 | |
| dc.date.issued | 2010 | |
| dc.date.submitted | 2010-07-30 | |
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Marie, R., Jensenius, H., Thaysen, J., and Christensen, C.B., “Adsorption kinetics and mechanical properties of thiol-modified DNA-oligos on gold investigated by microcantilever sensors”, Ultramicroscopy, Vol. 91, pp. 29-36, 2002. Mello L.D., and Kubota, L.T., “Review of the Use of Biosensors as Analytical Tools in the Food and Drink in Biomolecular Sensors”, Science, Vol. 311, pp. 1592-1595, 2006. Moulin, A.M., O’Shea, S.J., and Welland, M.E., “Microcantilever-based biosensors”, Ultramicroscopy, Vol. 82, pp. 23-31, 2000. Ramadan, M.A.M., Shrive, A.K., Holden, D., Myles, A.A., Volanakis, J.E., DeLucas, L.J., and Greenhough, T.J., “The Three-dimensional Structure of Calcium-Depleted Human C-reactive protein From Perfectly Twinned Crystals” Acta Crystallographica Section D, Vol. 58, pp. 992-1001, 2002. Rasmussen, P.A., Thaysen, J., Hansen, O., Eriksen, S.C., and Boisen, A., “ Optimised Cantilever Biosensor With Piezoresistive Read-out”, Ultramicroscopy, Vol. 97, pp. 371-376, 2003. Ridker, P.M., “High-Sensitivity C-Reactive Protein Potential Adjunct for Global Risk Assessment in the Primary Prevention of Cardiovascular Disease” Circulation, Vol. 103, pp. 1813-1818, 2001. Ridker, P.M., Glynn, R.J., and Hennekens, C.H., “C-Reactive Protein Adds to the Predictive Value of Total and HDL Cholesterol in Determining Risk of First Myocardial Infarction”, Circulation, Vol.97, No.20, pp. 2007-2011, 1998. Scheller, F., and Schubert, F., Biosensors, Elsevier Science Publishing Inc., New York, USA, 1992. Suresh, M.V., Singh, S.K., and Agrawal, A., “Interaction of Calcium-Bound C-Reactive Protein with Fibronectin Is Controlled by pH” The Journal of Biological Chemistry, Vol. 279, pp. 52552-52557, 2004. Thaysen, J., Boisen, A., Hansen, O., and Bouwstra, S., “Atomic Force Microscopy Probe with Piezoresistive Read-out and a Highly Symmetrical Wheatstone Bridge Arrangement”, Sensors and Actuators A, Vol. 83, pp.47-53, 2000. 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Ziegler, C., “Cantilever-based Biosensors”, Analytical and Bioanalytical Chemistry, Vol. 379, pp. 946-959, 2004. http://www.life.nctu.edu.tw/~mb 林清泉， “臨床血清免疫學”，第二版，藝軒出版社，1997。 行政院國家科學委員會精密儀器發展中心出版，”微機電系統技術與應用”，2003。 游育諺，高靈敏度之壓阻式微懸臂梁生物感測器應用於蛋白質分子之即時檢測，國立台灣大學應用力學所碩士論文，2006。 | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/46067 | - |
| dc.description.abstract | 本研究是以原子力顯微鏡與光纖干涉量測技術為架構，將微懸臂樑生物感測器應用於C反應蛋白之檢測，不僅可以簡化龐大的實驗設備，也可以增進量測的穩定性，光纖干涉儀在量測過程中不容易受到外界環境而影響實驗結果。本實驗藉由化學表面修飾，在微懸臂樑上鍵結C反應蛋白。在鍵結的過程中，將微懸臂樑生物感測器浸泡於含有待鍵結分子之溶液，再將之取出風乾，以進行共振頻的量測。在量測微懸臂樑的共振頻之前，須將微懸臂樑浸泡於去離子水中，否則將會因為磷酸鹽生物緩衝液乾燥之後在懸臂樑上留下結晶而影響光纖干涉儀量測結果。本文共檢測四種不同C反應蛋白溶液之濃度，分別為1、10、100以及1000ug/mL，每種濃度之共振頻停止變化的時間約在240至270分鐘後。在不同的濃度下，總共振頻的偏移量也不同，其變化量分別為117.6、144、211以及232.8 Hz。根據實驗結果，儘管C反應蛋白濃度不同，在鍵結開始後的30分鐘共振頻的變化皆為其總變化量的70%以上，表示在本研究所獲致的成果，在短時間內就可以達到檢測的效果。 | zh_TW |
| dc.description.abstract | Resonant based micro-cantilever was applied for the detection of C-reactive protein based on the technology of fiber interferometry. The vast apparatus was simplified, and the stability was increased. Resonant frequency shift of the micro-cantilever measured by fiber interferometry was remained undisturbed during experimental procedure.C-reactive protein was immobilized on micro-cantilever using surface micromachining technique. Micro-cantilever was immersed in the solution containing molecules which were about to be bonded, and parched before measuring the resonant frequency. The crystalline solid from dried PBS on surface of the micro-cantilever will affect the measurement and cause erroneousness. The D.I. water washout process was employed to fix the issue. The concentrations of C-reactive protein solution were 1, 10, 100 and 1000 ug/mL, and total resonant frequency shift were 117.6, 144, 211 and 232.8 Hz respectively. A distinct change in the resonant frequency of micro-cantilever was observed as a function of time. Resonant frequency decreased rapidly over 70% from initial value in the first 30 minutes of C-reactive protein antigen-antibody interaction and attained 95% after 240 minutes in saturation. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-15T04:52:54Z (GMT). No. of bitstreams: 1 ntu-99-R97543075-1.pdf: 2396582 bytes, checksum: 12d40a387cb7d9620050c538b22c75ce (MD5) Previous issue date: 2010 | en |
| dc.description.tableofcontents | 論文口試委員審定書 i
致謝 i 摘要 ii Abstract iii 目錄 iv 表目錄 vii 圖目錄 viii 符號說明 xi 第一章 緒論 1 1-1前言 1 1-2研究動機及目的 2 1-3文獻回顧 3 1-3-1微懸臂樑感測器之發展背景 3 1-3-2 C反應蛋白簡介 4 1-4研究方法 7 1-5論文架構 8 第二章 生物感測器 9 2-1生物感測器基本工作原理 9 2-2生物感測器之分類 10 2-3分子親和力與特異性鍵結 11 2-4微懸臂樑轉換元件之機制 12 2-4-1共振式感測器 13 2-5壓阻式與光學式之量測法 14 2-5-1壓阻式量測法 14 2-5-2光學式量測法 15 第三章 實驗設備與架構 17 3-1實驗架構 17 3-2實驗流程 17 3-3原子力顯微鏡 19 3-4光干涉技術 20 3-4-1壓電致動器驅動電路設計 20 3-4-2光纖干涉儀設計 21 第四章 實驗結果與討論 23 4-1重複性測試 23 4-2未泡水修飾之實驗結果 23 4-3泡水修飾 25 4-3-1乾燥時間對實驗之影響 25 4-4不同濃度下共振頻偏移 26 4-4-1短時間鍵結之共振頻偏移 28 第五章 結論與未來展望 29 5-1結論 29 5-2未來展望 31 參考文獻 32 附表 36 附圖 40 表目錄 表1-1 C反應蛋白出現的疾病......................................................36 表2-1 生物感測器之辨識分類......................................................37 表4-1 微懸臂樑生物感測器資料......................................................38 表4-2 共振頻偏移整理......................................................39 圖目錄 圖1-1 動物細胞膜之構造................................40 圖1-2 全球蛋白質藥物市場......................................................40 圖1-3 原子力顯微鏡示意圖......................................................41 圖1-4 常見的掃描式探針顯微鏡......................................................41 圖1-5 C反應蛋白之構型示意圖......................................................42 圖1-6 C反應蛋白與高密度脂蛋白之相對危險比例...........42 圖2-1 生物感測器之架構與常見的轉換器..................43 圖2-2 懸臂樑生物感測器之轉換器........................44 圖2-3 壓阻式微懸臂樑生物感測器........................44 圖2-4 壓組式微懸臂樑感測器換能機制....................45 圖2-5 光槓桿量測技術..................................45 圖2-6 雷射都卜勒光學振動量測儀之光路架構..............46 圖2-7 光干涉技術量測模組示意圖........................46 圖3-1 生物感測器與夾艙................................47 圖3-2 實驗架構........................................47 圖3-3 微懸臂樑表面化學修飾之流程......................48 圖3-4 (a)尚未放置生物感測器之夾艙 (b)固定生物感測器之夾.......48 圖3-5 (a)探頭，(b)vortex mixer........................49 圖3-6 電控箱及其顯示螢幕..............................49 圖3-7 光纖干涉儀干涉訊號..............................50 圖3-8 光纖干涉儀之架構圖..............................50 圖3-9 不同頻率下干涉儀所量測到的干涉訊號............. 51 圖3-10 微懸臂樑掃頻之結果..............................51 圖4-1 微懸臂樑生物感測器..............................52 圖4-2 儀器重複性測試..................................52 圖4-3 未泡水之共振頻偏移，C反應蛋白之濃度為10 mg/mL...53 圖4-4 浸泡磷酸鹽生物緩衝液之實驗對照組................53 圖4-5 浸泡去離子水以淡化磷酸鹽生物緩衝液之實驗對照組..54 圖4-6 泡去離子水與未泡之實驗對照......................54 圖4-7 乾燥時間對共振頻之影響..........................55 圖4-8 C反應蛋白溶液未均勻混合，濃度為1000 ug/mL.......55 圖4-9 C反應蛋白溶液未均勻混合，濃度為100 ug/mL........56 圖4-10(a)-(d) C反應蛋白濃度為1000、100、10以及1 ug/mL之實驗結果....................................................57 圖4-11 不同濃度下無因次化之共振頻變化..................59 圖4-12(a)-(d) C反應蛋白濃度為1000、100、10 以及1 ug/mL鍵結30分內之實驗結果.....................................60 圖4-13 30分鐘內，不同濃度下無因次化之共振頻變化........62 圖4-14 以無因次化之頻率變化為縱軸、濃度(X)之對數為橫軸，分別在T=5、10、30min.之關係...............................62 圖5-1 利用聚焦離子束於微懸臂樑上蝕刻流道..............63 | |
| dc.language.iso | zh-TW | |
| dc.subject | 光纖干涉儀 | zh_TW |
| dc.subject | 生物感測器 | zh_TW |
| dc.subject | 共振頻變化 | zh_TW |
| dc.subject | fiber interferometry | en |
| dc.subject | Bio-sensor | en |
| dc.subject | Resonant based micro-cantilever | en |
| dc.title | 應用共振式微懸臂樑感測器量測C反應蛋白之研究 | zh_TW |
| dc.title | Detection of C-Reactive Protein Using Resonance Micro-cantilever Beam Sensor | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 98-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 吳光鐘(Kuang-Chong Wu),張正憲(Jeng-Shian Chang),戴鴻名(Hong-Ming Dai) | |
| dc.subject.keyword | 生物感測器,共振頻變化,光纖干涉儀, | zh_TW |
| dc.subject.keyword | Bio-sensor,Resonant based micro-cantilever,fiber interferometry, | en |
| dc.relation.page | 63 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2010-07-30 | |
| dc.contributor.author-college | 工學院 | zh_TW |
| dc.contributor.author-dept | 應用力學研究所 | zh_TW |
| 顯示於系所單位： | 應用力學研究所 | |
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