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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 王兆麟 | |
dc.contributor.author | Meng-Hsun Lin | en |
dc.contributor.author | 林孟勳 | zh_TW |
dc.date.accessioned | 2021-06-15T04:49:17Z | - |
dc.date.available | 2010-08-05 | |
dc.date.copyright | 2010-08-05 | |
dc.date.issued | 2010 | |
dc.date.submitted | 2010-08-02 | |
dc.identifier.citation | 1. Adams MA, Hutton WC. The effect of posture on the fluid content of lumbar intervertebral discs. Spine 1983;8:665-71.
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Tissue Engineering 2003;9:667-77. 14. Hutton WC, Elmer WA, Boden SD, et al. The effect of hydrostatic pressure on intervertebral disc metabolism. Spine 1999;24:1507-15. 15. Jensen MC, Brant-Zawadzki MN, Obuchowski N, et al. Magnetic resonance imaging of the lumbar spine in people without back pain. New England Journal of Medicine 1994;331:69-73. 16. Junger S, Gantenbein-Ritter B, Lezuo P, et al. Effect of limited nutrition on in situ intervertebral disc cells under simulated-physiological loading. Spine 2009;34:1264-71. 17. Katz MM, Hargens AR, Garfin SR. Intervertebral disc nutrition. Diffusion versus convection. Clinical Orthopaedics & Related Research 1986:243-5. 18. Lee CR, Iatridis JC, Poveda L, et al. In vitro organ culture of the bovine intervertebral disc: effects of vertebral endplate and potential for mechanobiology studies. Spine 2006;31:515-22. 19. Luoma K, Riihimaki H, Luukkonen R, et al. Low back pain in relation to lumbar disc degeneration. Spine 2000;25:487-92. 20. Moore RJ. The vertebral end-plate: what do we know? European Spine Journal 2000;9:92-6. 21. Moore RJ. The vertebral endplate: disc degeneration, disc regeneration. European Spine Journal 2006;15 Suppl 3:S333-7. 22. Moore RJ, Vernon-Roberts B, Fraser RD, et al. The origin and fate of herniated lumbar intervertebral disc tissue. Spine 1996;21:2149-55. 23. Mwale F, Iordanova M, Demers CN, et al. Biological evaluation of chitosan salts cross-linked to genipin as a cell scaffold for disk tissue engineering. Tissue Engineering 2005;11:130-40. 24. Rajasekaran S, Babu JN, Arun R, et al. ISSLS prize winner: A study of diffusion in human lumbar discs: a serial magnetic resonance imaging study documenting the influence of the endplate on diffusion in normal and degenerate discs. Spine 2004;29:2654-67. 25. Richardson SM, Hoyland JA, Mobasheri R, et al. Mesenchymal stem cells in regenerative medicine: opportunities and challenges for articular cartilage and intervertebral disc tissue engineering. 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Spine 2004;29:2700-9. 32. van der Werf M, Lezuo P, Maissen O, et al. Inhibition of vertebral endplate perfusion results in decreased intervertebral disc intranuclear diffusive transport. Journal of Anatomy 2007;211:769-74. 33. Wilke HJ, Kettler A, Claes LE. Are sheep spines a valid biomechanical model for human spines? Spine 1997;22:2365-74. 34. Wilke HJ, Neef P, Caimi M, et al. New in vivo measurements of pressures in the intervertebral disc in daily life. Spine 1999;24:755-62. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/45936 | - |
dc.description.abstract | 目的:(一)開發一套適用於全椎間盤培養系統、可模擬生物體內養分循環及動態載重之生物反應器。(二) 探討椎終板、培養液循環、負載型態等三個因子對椎間盤內部分子傳輸的影響。
背景簡介:全椎間盤培養系統是目前以體外實驗探討椎間盤生化反應與力學性質關係的最佳選擇,可用於測試椎間盤藥物與組織工程新技術的效能。為模擬椎間盤的自然生長環境,全椎間盤培養系統需配置生物反應器,以提供椎間盤類似於生物體內的受力狀態與養分代謝機制。椎間盤於受力時所產生的組織間液流動,以及生物反應器驅動培養液循環時所產生的壓力可促進外來分子於椎間盤內部的傳遞,但脊椎終板的存在可能會抵消培養液的驅動力。藉著生物反應器的開發,可探討椎終板、培養液循環機制、負載型態對於椎間盤內部分子傳遞的影響。 材料與方法:(一)生物反應器之開發:生物反應器包含培養室、載重模組與培養液循環模組。培養室為一內徑40mm,高30mm的透明空心圓柱,管壁材質為壓克力,上、下各接一多孔性不鏽鋼濾板,孔徑為50um,濾板外接由鐵氟龍製成之上、下底座,中間有孔道供矽膠管通過。矽膠管繞過蠕動幫浦後連接盛裝培養液之離心管,蠕動幫浦以擠壓矽膠管的方式將培養液以固定流速(0.15-2.95ml/min)從上方孔道送入培養室,再從下方輸出。培養室的上方固定座以載重傳輸桿外接由汽壓缸、電池閥及時間閃爍器組成之載重模組,可輸出之最大力量為1800N,最快頻率為1Hz。 (二)將螢光素納溶液0.25ml注射至胸椎椎間盤後,將椎間盤平均分成三組,分別探討(1)脊椎終板、(2)培養液循環、(3)負載型態等因子對椎間盤內部分子傳輸之影響。將椎間盤放入生物反應器一小時,以2.95 ml/min的流速循環食鹽水,並分別輸出靜態載重(0.2MPa)及動態載重(0.2-0.8MPa,0.2Hz)。一小時後將椎間盤取出切半,使用螢光攝影機觀察螢光染劑的擴散面積,並以獨立t檢定進行統計檢驗。 結果:(1)生長板的有無對螢光染劑的擴散面積沒有顯著性影響(p=0.149)。(2)培養液循環的有無,對螢光染劑的擴散面積於靜態載重組(p=0.184)及動態載重組(p=0.299)均無顯著性影響。(3)不論循環模組關閉(p=0.000)或開啟(p=0.034),動態負載下的螢光染劑擴散面積皆大於靜態負載時的螢光染劑擴散面積。 結論:使用本研究開發之生物反應器,椎間盤內部分子傳輸不受椎終板所影響。循環系統對於椎間盤內部分子的傳輸沒有明顯效果。動態負載可提供椎間盤內部液體對流功能,故能加快椎間盤內分子傳輸。 | zh_TW |
dc.description.provenance | Made available in DSpace on 2021-06-15T04:49:17Z (GMT). No. of bitstreams: 1 ntu-99-R97548041-1.pdf: 1775084 bytes, checksum: d67ac227e3f001781fe3006a13582657 (MD5) Previous issue date: 2010 | en |
dc.description.tableofcontents | 中文摘要 i
ABSTRACT iii 表目錄 vii 第一章 前言 1 1.1椎間盤之基本構造 1 1.2椎間盤再生研究 3 1.3新型式椎間盤培養技術 4 1.4研究目的 8 第二章 實驗機台設計 9 2.1椎間盤培養系統 9 2.2生物反應器 9 2.3動力模組 12 2.4控制模組 13 2.5養分循環系統 15 第三章 實驗方法 17 3.1試樣準備 17 3.2實驗設計 17 3.3影像分析 20 第四章 結果 22 4.1椎間盤培養系統 22 4.2染劑分佈面積 23 4.3椎間環擴散速度 24 4.4椎終板擴散速度 25 第五章 討論 27 5.1椎間盤養分代謝 27 5.2試樣之影響 27 5.3載重系統之影響 28 5.4養分循環系統之影響 28 5.5實驗限制 29 參考文獻 31 | |
dc.language.iso | zh-TW | |
dc.title | 全椎間盤之生物反應器開發 | zh_TW |
dc.title | Development of a Whole Disc Bioreactor | en |
dc.type | Thesis | |
dc.date.schoolyear | 98-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 趙本秀,蕭仲凱,楊台鴻 | |
dc.subject.keyword | 生物反應器,培養系統,椎間盤,養分,代謝, | zh_TW |
dc.subject.keyword | bioreactor,culture system,intervertebral disc,nutrient,metabolic, | en |
dc.relation.page | 33 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2010-08-03 | |
dc.contributor.author-college | 工學院 | zh_TW |
dc.contributor.author-dept | 醫學工程學研究所 | zh_TW |
顯示於系所單位: | 醫學工程學研究所 |
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