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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
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dc.contributor.advisor | 錢宗良 | |
dc.contributor.author | Yun-Chien Hsieh | en |
dc.contributor.author | 謝昀蒨 | zh_TW |
dc.date.accessioned | 2021-06-15T04:48:48Z | - |
dc.date.available | 2013-09-09 | |
dc.date.copyright | 2010-09-09 | |
dc.date.issued | 2010 | |
dc.date.submitted | 2010-08-03 | |
dc.identifier.citation | Al-Hajj, M., and Clarke, M.F. (2004). Self-renewal and solid tumor stem cells. Oncogene 23, 7274-7282.
Anciaux, K., Van Dommelen, K., Nicolai, S., Van Mechelen, E., and Slegers, H. (1997). Cyclic AMP-mediated induction of the glial fibrillary acidic protein is independent of protein kinase A activation in rat C6 glioma. J Neurosci Res 48, 324-333. Audesirk, G., Cabell, L., and Kern, M. (1997). Modulation of neurite branching by protein phosphorylation in cultured rat hippocampal neurons. Brain Res Dev Brain Res 102, 247-260. Backhovens, H., Gheuens, J., and Slegers, H. (1987). Expression of glial fibrillary acidic protein in rat C6 glioma relates to vimentin and is independent of cell-cell contact. J Neurochem 49, 348-354. Bacskai, B.J., Hochner, B., Mahaut-Smith, M., Adams, S.R., Kaang, B.K., Kandel, E.R., and Tsien, R.Y. (1993). Spatially resolved dynamics of cAMP and protein kinase A subunits in Aplysia sensory neurons. Science 260, 222-226. Bang, B.E., Ericsen, C., and Aarbakke, J. (1994). Effects of cAMP and cGMP elevating agents on HL-60 cell differentiation. Pharmacol Toxicol 75, 108-112. Benda, P., Lightbody, J., Sato, G., Levine, L., and Sweet, W. (1968). Differentiated rat glial cell strain in tissue culture. Science 161, 370-371. Bianchi, M.G., Gazzola, G.C., Tognazzi, L., and Bussolati, O. (2008). C6 glioma cells differentiated by retinoic acid overexpress the glutamate transporter excitatory amino acid carrier 1 (EAAC1). Neuroscience 151, 1042-1052. Black, M.M., and Zachrau, R.E. (1991). In situ carcinoma-associated immunogenicity: therapeutic and prophylactic implications in breast cancer patients. Adv Cancer Res 56, 105-131. Bookout, A.L., Cummins, C.L., Mangelsdorf, D.J., Pesola, J.M., and Kramer, M.F. (2006). High-throughput real-time quantitative reverse transcription PCR. Curr Protoc Mol Biol Chapter 15, Unit 15 18. Burger, H.G. (1991). Inhibin: clinical physiology and cancer diagnosis. Reprod Fertil Dev 3, 227-231. Buzanska, L., Ruiz, A., Zychowicz, M., Rauscher, H., Ceriotti, L., Rossi, F., Colpo, P., Domanska-Janik, K., and Coecke, S. (2009). Patterned growth and differentiation of human cord blood-derived neural stem cells on bio-functionalized surfaces. Acta Neurobiol Exp (Wars) 69, 24-36. Cabell, L., and Audesirk, G. (1993). Effects of selective inhibition of protein kinase C, cyclic AMP-dependent protein kinase, and Ca(2+)-calmodulin-dependent protein kinase on neurite development in cultured rat hippocampal neurons. Int J Dev Neurosci 11, 357-368. Chang, L., and Goldman, R.D. (2004). Intermediate filaments mediate cytoskeletal crosstalk. Nat Rev Mol Cell Biol 5, 601-613. Chrenek, P., Grossmann, R., and Sirotkin, A.V. (2010). The cAMP analogue, dbcAMP affects release of steroid hormones by cultured rabbit ovarian cells and their response to FSH, IGF-I and ghrelin. Eur J Pharmacol 640, 202-205. Clarke, M.F., and Fuller, M. (2006). Stem cells and cancer: two faces of eve. Cell 124, 1111-1115. Coulombe, P.A., and Wong, P. (2004). Cytoplasmic intermediate filaments revealed as dynamic and multipurpose scaffolds. Nat Cell Biol 6, 699-706. Dalerba, P., Cho, R.W., and Clarke, M.F. (2007). Cancer stem cells: models and concepts. Annu Rev Med 58, 267-284. Das, A., Banik, N.L., and Ray, S.K. (2007). Differentiation decreased telomerase activity in rat glioblastoma C6 cells and increased sensitivity to IFN-gamma and taxol for apoptosis. Neurochem Res 32, 2167-2183. De Filippis, L., Lamorte, G., Snyder, E.Y., Malgaroli, A., and Vescovi, A.L. (2007). A novel, immortal, and multipotent human neural stem cell line generating functional neurons and oligodendrocytes. Stem Cells 25, 2312-2321. Galli, F., Stabile, A.M., Betti, M., Conte, C., Pistilli, A., Rende, M., Floridi, A., and Azzi, A. (2004). The effect of alpha- and gamma-tocopherol and their carboxyethyl hydroxychroman metabolites on prostate cancer cell proliferation. Arch Biochem Biophys 423, 97-102. Gilbertson, R.J., and Gutmann, D.H. (2007). Tumorigenesis in the brain: location, location, location. Cancer Res 67, 5579-5582. Gilbertson, R.J., and Rich, J.N. (2007). Making a tumour's bed: glioblastoma stem cells and the vascular niche. Nat Rev Cancer 7, 733-736. Grobben, B., De Deyn, P.P., and Slegers, H. (2002). Rat C6 glioma as experimental model system for the study of glioblastoma growth and invasion. Cell Tissue Res 310, 257-270. Hemmati, H.D., Nakano, I., Lazareff, J.A., Masterman-Smith, M., Geschwind, D.H., Bronner-Fraser, M., and Kornblum, H.I. (2003). Cancerous stem cells can arise from pediatric brain tumors. Proc Natl Acad Sci U S A 100, 15178-15183. Herrmann, H., and Aebi, U. (2000). Intermediate filaments and their associates: multi-talented structural elements specifying cytoarchitecture and cytodynamics. Curr Opin Cell Biol 12, 79-90. Hirschmann-Jax, C., Foster, A.E., Wulf, G.G., Nuchtern, J.G., Jax, T.W., Gobel, U., Goodell, M.A., and Brenner, M.K. (2004). A distinct 'side population' of cells with high drug efflux capacity in human tumor cells. Proc Natl Acad Sci U S A 101, 14228-14233. Iacovitti, L., Stull, N.D., and Jin, H. (2001). Differentiation of human dopamine neurons from an embryonic carcinomal stem cell line. Brain Res 912, 99-104. Ignatova, T.N., Kukekov, V.G., Laywell, E.D., Suslov, O.N., Vrionis, F.D., and Steindler, D.A. (2002). Human cortical glial tumors contain neural stem-like cells expressing astroglial and neuronal markers in vitro. Glia 39, 193-206. Inagaki, M., Nakamura, Y., Takeda, M., Nishimura, T., and Inagaki, N. (1994). Glial fibrillary acidic protein: dynamic property and regulation by phosphorylation. Brain Pathol 4, 239-243. Karmakar, S., Olive, M.F., Banik, N.L., and Ray, S.K. (2007). Intracranial stereotaxic cannulation for development of orthotopic glioblastoma allograft in Sprague-Dawley rats and histoimmunopathological characterization of the brain tumor. Neurochem Res 32, 2235-2242. Kaukel, E., Fuhrmann, U., and Hilz, H. (1972). Divergent action of cAMP and dibutyryl cAMP on macromolecular synthesis in HeLa S3 cultures. Biochem Biophys Res Commun 48, 1516-1524. Kim, E.J., Kwon, K.J., Park, J.Y., Lee, S.H., Moon, C.H., and Baik, E.J. (2002). Neuroprotective effects of prostaglandin E2 or cAMP against microglial and neuronal free radical mediated toxicity associated with inflammation. J Neurosci Res 70, 97-107. Koch, M., May, U., Kuhns, S., Drechsler, H., Adam, N., Hattermann, K., Wirtz, S., Rose-John, S., and Scheller, J. (2007). Interleukin 27 induces differentiation of neural C6-precursor cells into astrocytes. Biochem Biophys Res Commun 364, 483-487. Kondo, T., Setoguchi, T., and Taga, T. (2004). Persistence of a small subpopulation of cancer stem-like cells in the C6 glioma cell line. Proc Natl Acad Sci U S A 101, 781-786. Kondo, T. (2006). Brain cancer stem-like cells. Eur J Cancer 42, 1237-1242. Lando, M., Abemayor, E., Verity, M.A., and Sidell, N. (1990). Modulation of intracellular cyclic adenosine monophosphate levels and the differentiation response of human neuroblastoma cells. Cancer Res 50, 722-727. Linskey, M.E., and Gilbert, M.R. (1995). Glial differentiation: a review with implications for new directions in neuro-oncology. Neurosurgery 36, 1-21; discussion 21-22. Livak, K.J., and Schmittgen, T.D. (2001). Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods 25, 402-408. Lopez-Toledano, M.A., Redondo, C., Lobo, M.V., Reimers, D., Herranz, A.S., Paino, C.L., and Bazan, E. (2004). Tyrosine hydroxylase induction by basic fibroblast growth factor and cyclic AMP analogs in striatal neural stem cells: role of ERK1/ERK2 mitogen-activated protein kinase and protein kinase C. J Histochem Cytochem 52, 1177-1189. Maric, D., Maric, I., Chang, Y.H., and Barker, J.L. (2003). Prospective cell sorting of embryonic rat neural stem cells and neuronal and glial progenitors reveals selective effects of basic fibroblast growth factor and epidermal growth factor on self-renewal and differentiation. J Neurosci 23, 240-251. Marzi, I., D'Amico, M., Biagiotti, T., Giunti, S., Carbone, M.V., Fredducci, D., Wanke, E., and Olivotto, M. (2007). Purging of the neuroblastoma stem cell compartment and tumor regression on exposure to hypoxia or cytotoxic treatment. Cancer Res 67, 2402-2407. Matsumura, N., Yoshida, N., Ohta, A., Miyamoto, Y., and Hisatsune, T. (2003). Neural precursor cells from adult mouse cerebral cortex differentiate into both neurons and oligodendrocytes. Cytotechnology 43, 19-25. McCall, M.A., Gregg, R.G., Behringer, R.R., Brenner, M., Delaney, C.L., Galbreath, E.J., Zhang, C.L., Pearce, R.A., Chiu, S.Y., and Messing, A. (1996). Targeted deletion in astrocyte intermediate filament (Gfap) alters neuronal physiology. Proc Natl Acad Sci U S A 93, 6361-6366. Messens, J., and Slegers, H. (1992). Synthesis of glial fibrillary acidic protein in rat C6 glioma in chemically defined medium: cyclic AMP-dependent transcriptional and translational regulation. J Neurochem 58, 2071-2080. Otte, A.P., van Run, P., Heideveld, M., van Driel, R., and Durston, A.J. (1989). Neural induction is mediated by cross-talk between the protein kinase C and cyclic AMP pathways. Cell 58, 641-648. Park, D.M., and Rich, J.N. (2009). Biology of glioma cancer stem cells. Mol Cells 28, 7-12. Parker, K.K., Norenberg, M.D., and Vernadakis, A. (1980). 'Transdifferentiation' of C6 glial cells in culture. Science 208, 179-181. Raff, M. (1996). Neural development: mysterious no more? Science 274, 1063. Reya, T., Morrison, S.J., Clarke, M.F., and Weissman, I.L. (2001). Stem cells, cancer, and cancer stem cells. Nature 414, 105-111. Rutka, J.T., Murakami, M., Dirks, P.B., Hubbard, S.L., Becker, L.E., Fukuyama, K., Jung, S., Tsugu, A., and Matsuzawa, K. (1997). Role of glial filaments in cells and tumors of glial origin: a review. J Neurosurg 87, 420-430. Sampson, J.H. (1999). The Preuss Foundation Seminar on vaccine therapy for malignant primary brain tumors. February 15-17, 1998, La Jolla, Calif. Neuro Oncol 1, 33-42. Sasaki, T., Yamazaki, K., Yamori, T., and Endo, T. (2002). Inhibition of proliferation and induction of differentiation of glioma cells with Datura stramonium agglutinin. Br J Cancer 87, 918-923. Sawyers, C.L., Denny, C.T., and Witte, O.N. (1991). Leukemia and the disruption of normal hematopoiesis. Cell 64, 337-350. Schwartz, J.H. (2001). The many dimensions of cAMP signaling. Proc Natl Acad Sci U S A 98, 13482-13484. Schwartz, P.H., Bryant, P.J., Fuja, T.J., Su, H., O'Dowd, D.K., and Klassen, H. (2003). Isolation and characterization of neural progenitor cells from post-mortem human cortex. J Neurosci Res 74, 838-851. Segovia, J., Lawless, G.M., Tillakaratne, N.J., Brenner, M., and Tobin, A.J. (1994). Cyclic AMP decreases the expression of a neuronal marker (GAD67) and increases the expression of an astroglial marker (GFAP) in C6 cells. J Neurochem 63, 1218-1225. Setoguchi, T., Taga, T., and Kondo, T. (2004). Cancer stem cells persist in many cancer cell lines. Cell Cycle 3, 414-415. Shao, X.M., Ge, Q., and Feldman, J.L. (2003). Modulation of AMPA receptors by cAMP-dependent protein kinase in preBotzinger complex inspiratory neurons regulates respiratory rhythm in the rat. J Physiol 547, 543-553. Shen, G., Shen, F., Shi, Z., Liu, W., Hu, W., Zheng, X., Wen, L., and Yang, X. (2008). Identification of cancer stem-like cells in the C6 glioma cell line and the limitation of current identification methods. In Vitro Cell Dev Biol Anim 44, 280-289. Shi, Y., Do, J.T., Desponts, C., Hahm, H.S., Scholer, H.R., and Ding, S. (2008). A combined chemical and genetic approach for the generation of induced pluripotent stem cells. Cell Stem Cell 2, 525-528. Singh, S.K., Clarke, I.D., Terasaki, M., Bonn, V.E., Hawkins, C., Squire, J., and Dirks, P.B. (2003). Identification of a cancer stem cell in human brain tumors. Cancer Res 63, 5821-5828. Takanaga, H., Yoshitake, T., Hara, S., Yamasaki, C., and Kunimoto, M. (2004). cAMP-induced astrocytic differentiation of C6 glioma cells is mediated by autocrine interleukin-6. J Biol Chem 279, 15441-15447. Tan, B.T., Park, C.Y., Ailles, L.E., and Weissman, I.L. (2006). The cancer stem cell hypothesis: a work in progress. Lab Invest 86, 1203-1207. Tardy, M., Fages, C., Riol, H., LePrince, G., Rataboul, P., Charriere-Bertrand, C., and Nunez, J. (1989). Developmental expression of the glial fibrillary acidic protein mRNA in the central nervous system and in cultured astrocytes. J Neurochem 52, 162-167. Waltereit, R., and Weller, M. (2003). Signaling from cAMP/PKA to MAPK and synaptic plasticity. Mol Neurobiol 27, 99-106. Yamamoto, M., Urakubo, T., Tominaga-Yoshino, K., and Ogura, A. (2005). Long-lasting synapse formation in cultured rat hippocampal neurons after repeated PKA activation. Brain Res 1042, 6-16. Yeung, H.Y., Chan, D.K., Mak, N.K., Wagner, G.F., and Wong, C.K. (2003). Identification of signal transduction pathways that modulate dibutyryl cyclic adenosine monophosphate activation of stanniocalcin gene expression in neuroblastoma cells. Endocrinology 144, 4446-4452. Yu, S.C., Ping, Y.F., Yi, L., Zhou, Z.H., Chen, J.H., Yao, X.H., Gao, L., Wang, J.M., and Bian, X.W. (2008). Isolation and characterization of cancer stem cells from a human glioblastoma cell line U87. Cancer Lett 265, 124-134. Zheng, X., Shen, G., Yang, X., and Liu, W. (2007). Most C6 cells are cancer stem cells: evidence from clonal and population analyses. Cancer Res 67, 3691-3697. Zhou, D., Jiang, X., Xu, R., Cai, Y., Hu, J., Xu, G., Zou, Y., and Zeng, Y. (2008). Assessing the cytoskeletal system and its elements in C6 glioma cells and astrocytes by atomic force microscopy. Cell Mol Neurobiol 28, 895-905. Zhou, X.D., Wang, X.Y., Qu, F.J., Zhong, Y.H., Lu, X.D., Zhao, P., Wang, D.H., Huang, Q.B., Zhang, L., and Li, X.G. (2009). Detection of cancer stem cells from the C6 glioma cell line. J Int Med Res 37, 503-510. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/45917 | - |
dc.description.abstract | 神經膠細胞瘤是一種極為常見的惡性腦瘤。目前的處理方式以手術切除及放射治療為主,然而治療效率並不高,其復發的情形及致死率依然居高不下。利用藥物誘導癌細胞分化可能將其導引為正常細胞狀態,是阻止惡化的一種方法。大鼠C6細胞株是常用的一種神經膠細胞瘤研究模式。C6細胞有極快速增殖的特性,並且擁有寡突膠細胞、星狀膠細胞、以及神經元的分化潛能。本實驗根據癌幹細胞的假說,將C6細胞利用球狀培養環境模擬接近癌幹細胞的情形,同時也用一般貼附培養的C6細胞一起比較,觀察二丁酰環腺嘌呤單核苷酸(dbcAMP)對C6細胞的誘導分化成效。
我們利用相位差顯微鏡觀察形態上的變化,並利用細胞免疫螢光染色、西方墨點轉漬、即時定量聚合酶連鎖反應等方法觀察蛋白質及核醣核酸的表現。經過dbcAMP處理,原本呈現多角型的細胞變得狹長,而伸出細長的突起,接近前趨膠細胞的形態。部份在球狀培養的細胞甚至呈現星狀,類似星狀膠細胞或神經元。在蛋白質及核醣核酸的表現上發現,代表星狀膠細胞的標記GFAP在dbcAMP處理後增加;有趣的發現是代表神經元的標識ßⅢ-tubulin在球狀培養的細胞增加表現;同時,代表神經幹細胞的nestin表現則相對減弱。 透過dbcAMP引發C6細胞分化的研究,我們認為實驗所使用的球狀培養細胞方式,能促使細胞擁有癌幹細胞的特質。這樣的模式將可運用在C6癌幹細胞起始狀態及分化路徑的研究。 | zh_TW |
dc.description.abstract | Glioma is one of the most common primary brain tumors. Its conventional treatments such as surgical resection or radiation fail to increase the survival of patients. Induction of differentiation is one way for controlling the malignant growth of glioma. Rat C6 glioma is a rapidly proliferating cell line with oligodendrocytic, astrocytic, and neuronal properties. Based on the cancer stem cell hypothesis, in this study, we use C6 tumor sphere cells, together with parental adhesion cells, to investigate the regulation of differentiation-inducing agent, N6-O'2-dibutyryl cAMP (dbcAMP).
The efficacy of treatment was examined by phase-contrast microscopy, Immunocytochemistry, Western blot, RT-PCR and Real Time Quantitative PCR. After dbcAMP treatment, morphological changes of C6 cells could be easily observed. Most of the adhesion cells showed bipolar shape, which are similar to glial progenitors. Compared with adhesion cells, sphere cells exposed to dbcAMP also extended processes, and some cells resulted in a stellar shape. The morphology more closely resembled mature astrocyte or neuron. Astrocytic marker glial fibrillary acidic protein (GFAP) expression increased in tumorsphere. Interestingly, the specific neuronal marker ßⅢ-tubulin immunopositive cells were also observed. Meanwhile, the neural stem cell marker nestin decreased relatively. Our results suggest that the formation of tumor sphere model, which has cancer stem-like cells potential, could be a suitable approach to identify the initiation and progression of rat C6 glioma cells. | en |
dc.description.provenance | Made available in DSpace on 2021-06-15T04:48:48Z (GMT). No. of bitstreams: 1 ntu-99-R97446001-1.pdf: 2303225 bytes, checksum: 35990c255cfe1880703817020f7c8c79 (MD5) Previous issue date: 2010 | en |
dc.description.tableofcontents | 中英文摘要 ………………………………1
緒論 ………………………………………4 材料與方法 ………………………………9 結果 ………………………………………15 討論 ………………………………………20 參考文獻 …………………………………23 圖表說明 …………………………………31 | |
dc.language.iso | en | |
dc.title | N6-O'2-dibutyryl cAMP對C6神經膠細胞瘤分化之調控 | zh_TW |
dc.title | N6-O'2-dibutyryl cAMP regulates the differentiation of C6 glioma cells | en |
dc.type | Thesis | |
dc.date.schoolyear | 98-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 盧國賢,歐陽品 | |
dc.subject.keyword | 神經膠細胞瘤,癌幹細胞, | zh_TW |
dc.subject.keyword | glioma,cancer stem cell,dbcAMP, | en |
dc.relation.page | 51 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2010-08-03 | |
dc.contributor.author-college | 醫學院 | zh_TW |
dc.contributor.author-dept | 解剖學暨生物細胞學研究所 | zh_TW |
顯示於系所單位: | 解剖學暨細胞生物學科所 |
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