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http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/45840完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 林文澧 | |
| dc.contributor.author | Heng-Ruei Shiu | en |
| dc.contributor.author | 許恆瑞 | zh_TW |
| dc.date.accessioned | 2021-06-15T04:47:06Z | - |
| dc.date.available | 2013-08-11 | |
| dc.date.copyright | 2010-08-11 | |
| dc.date.issued | 2010 | |
| dc.date.submitted | 2010-08-04 | |
| dc.identifier.citation | 1. Duck,FA.,Baker,AC., Starritt,HC.(1998).Ultrasound in medicine (Medical Sciences Series), Institute of Physics
2. Sboros,V.(2008). 'Response of contrast agents to ultrasound.' Advanced Drug Delivery Reviews 60(10): 1117-1136. 3. Frenkel,V.(2008). 'Ultrasound mediated delivery of drugs and genes to solid tumors.' Advanced Drug Delivery Reviews 60(10): 1193-1208. 4. Pitt,WG., Husseini,GA.,and Staples,BJ.(2004). 'Ultrasonic drug delivery – a general review. ' Expert Opin Drug Deliv 1(1): 37–56. 5. Husseini,GA., and Pitt,WG.(2008). 'The use of ultrasound and micelles in cancer treatment. ' J Nanosci Nanotechnol 8(5): 2205–2215. 6. Ferrara,KW.(2008). 'Driving delivery vehicles with ultrasound.' Advanced Drug Delivery Reviews 60(10): 1097-1102. 7. Tashjian,JA., Dewhirst MW.,et al.(2008). 'Rationale for and measurement of liposomal drug delivery with hyperthermia using non-invasive imaging techniques.' Int J Hyperthermia 24(1): 79–90. 8. Ponce,AM. ,Vujaskovic,Z.et al.(2006). 'Hyperthermia mediated liposomal drug delivery.' Int J Hyperthermia 22(3): 205–213. 9. Kikumori,T.,Kobayashi,T., et al. (2009). 'Anti-cancer effect of hyperthermia on breast cancer by magnetite nanoparticle-loaded anti-HER2 immunoliposomes.' Breast Cancer Research and Treatment 113(3): 435-441. 10.Kong,G.,Braun,RD., Dewhirst MW., et al.(2000) 'Hyperthermia enables tumor-specific nanoparticle delivery: effect of particle size. 'Cancer Res 60(16):4440-4445. 11. Lentacker, I., Geers,B., et al. (2009). 'Design and evaluation of doxorubicin-containing microbubbles for ultrasound-triggered doxorubicin delivery: cytotoxicity and mechanisms involved.' Mol Ther 18(1): 101-108 12. Pourtier-Manzanedo,A.,Vercamer,C.,et al. (2003). 'Expression of an Ets-1 dominant-negative mutant perturbs normal and tumor angiogenesis in a mouse ear model.' Oncogene 22(12): 1795-1806. 13. Tohru Hoshida, Naohide Isaka,et al.(2006). 'Imaging steps of lymphatic metastasis reveals that vascular endothelial growth factor-C increases metastasis by increasing delivery of cancer cells to lymph nodes:therapeutic implications.'Cancer Res 66(16): 8065-8075. 14. Coventry, BJ., Macardle,PJ.,et al. (1994). 'A technique for successful transplantation of tumours into ear-pouches of nude mice to maintain and study the tumour microenvironment.' Surgical Oncology 3(2):127-129. 15. Iwanaga, K., Tominaga,K.,et al. (2007). 'Local delivery system of cytotoxic agents to tumors by focused sonoporation.' Cancer Gene Ther 14(4): 354-363. 16. Bekeredjian, R., Kroll,RD., et al. (2007). 'Ultrasound targeted microbubble destruction increases capillary permeability in hepatomas.' Ultrasound in Medicine & Biology 33(10): 1592-1598. 17. Frenkel,V., Etherington,A., et al. (2006). 'Delivery of liposomal doxorubicin (Doxil) in a breast cancer tumor model: investigation of potential enhancement by pulsed-high intensity focused ultrasound exposure.' academic radiology 13(4): 469-479. 18. Hancock, HA., Smith,LH.,et al. (2009). 'Investigations into pulsed high-intensity focused ultrasound-enhanced delivery: preliminary evidence for a novel mechanism.' Ultrasound in Medicine & Biology 35(10): 1722-1736. 19. Mizrahi, N., Seliktar,D.,et al. (2007). 'Ultrasound-induced angiogenic response in endothelial cells.' ultrasound in medicine & biology 33(11): 1818-1829. 20. Ramli R, Reher P, Harris M, Meghji S, et al. (2009) 'The effect of ultrasound on angiogenesis: an in vivo study using the chick chorioallantoic membrane. 'Int J Oral Maxillofac Implants 24(4): 591-596. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/45840 | - |
| dc.description.abstract | 近年來有研究指出，藥物搭配超音波與微氣泡和藥物搭配熱治療兩者治療腫瘤的方式皆能強化藥物傳遞，進而增加藥物在腫瘤內的濃度，使腫瘤得到較好的治療效果。而本研究利用小鼠耳朵腫瘤模型(Mouse Ear Tumor Model)，使用奈米藥物Doxil、超音波與微氣泡及43℃加熱15分鐘之熱治療三種治療方式互相做搭配，探討在治療腫瘤的情形，總共治療八次，每兩天治療一次，治療前四次後，休息一個星期之後，再持續給予後四次的治療。研究結果觀察到真正對腫瘤治療比較有效的是奈米藥物Doxil搭配上超音波及微氣泡、奈米藥物Doxil搭配上43℃加熱15分鐘之熱治療、奈米藥物Doxil搭配上超音波及微氣泡又加再加上43℃加熱15分鐘之熱治療這三組；而以腫瘤生長狀態而言，又以奈米藥物Doxil搭配上超音波及微氣泡再加上43℃加熱15分鐘之熱治療和奈米藥物Doxil搭配上43℃加熱15分鐘之熱治療這兩組較有效，而奈米藥物Doxil搭配上超音波及微氣泡再加上43℃加熱15分鐘之熱治療甚至能使腫瘤逐漸變小到幾乎看不見，在小腫瘤的治療上有相當顯著的治療效果。 | zh_TW |
| dc.description.abstract | Studies show that ultrasound (US) with microbubbles (MB) or hyperthermia (HT) could enhance the delivery of nanoparticles into tumor tissues. In this research, we used mouse ear tumor model to study the effectiveness of nanodrug on the tumor treatment with the enhancement of ultrasound/ microbubbles and/or hyperthermia, and observed the tumor growth with microscopy. The treatment procedure was arranged with four successive treatments every other day, and then followed the same four successive treatments after a week off.Experimental results showed that treatment enhancement can be observed for nanodrug injection followed by MB/US, or HT (43℃, 15 min),or MB/US/HT. The treatment set with MB/US/HT following nanodrug injection can even make the tumor disappear. The results of this study indicates that MB/US/HT following the injection of nanodrug is a very promising method for effective tumor treatment. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-15T04:47:06Z (GMT). No. of bitstreams: 1 ntu-99-R97548049-1.pdf: 3711931 bytes, checksum: 736fcc0548e9d7f683a109676dac8729 (MD5) Previous issue date: 2010 | en |
| dc.description.tableofcontents | 誌謝…………………………………………………………………………………....I
摘要…………………………………………………………………………………..II Abstract……………………………………………………………….....III 目錄…………………………………………………………………………………..IV 圖目錄………………………………………………………………………………...V 第一章 緒論…………………………………………………………………….......1 1-1 超音波……………………………………………………………………..1 1-2 空蝕化效應………………………………………………………………..1 1-3 熱效應……………………………………………………………………..2 1-4 超音波加強藥物傳遞的機制……………………………………………..3 1-5 熱治療……………………………………………………………………..3 1-6 奈米抗癌藥物Doxil……………………………………………………....5 1-7 研究目的…………………………………………………………………..5 第二章 實驗設備與材料方法……………………………………………………...8 2-1 腫瘤細胞株………………………………………………………………..9 2-2 實驗動物…………………………………………………………………..9 2-3 抗癌藥……………………………………………………………………..9 2-4 超音波顯影劑……………………………………………………………..9 2-5 超音波設備………………………………………………………………10 2-6 超音波參數………………………………………………………………10 2-7 加熱系統…………………………………………………………………10 2-8 顯微鏡系統………………………………………………………………11 2-9 顯微鏡影像………………………………………………………………11 2-10數值分析方法…………………………………………………………….11 2-11 實驗設計與流程…………………………………………………………12 第三章 結果……………………………………………………………………….16 第四章 討論……………………………………………………………………….60 第五章 結論與未來工作………………………………………………………….63 參考文獻……………………………………………………………………………..64 圖目錄 圖1.1 慣性空蝕化的閥值…………………………………………………………..6 圖1.2 慣性空蝕化的現象示意圖………………………………………………......7 圖1.3 超音波強化藥物傳送示意圖………………………………………………..7 圖1.4 熱治療強化藥物傳送的示意圖……………………………………………..8 圖2.1 超音波設備…………………………………………………………………13 圖2.2 超音波能量分布圖…………………………………………………………13 圖2.3 加熱系統……………………………………………………………………14 圖2.4 螢光解剖顯微鏡……………………………………………………………14 圖2.5 顯微鏡影像…………………………………………………………………15 圖2.6 實驗流程圖…………………………………………………………………15 圖3.1 Control組在顯微鏡下觀察到的影像……………………………………..16 圖3.2 Control組在顯微鏡下觀察到的影像……………………………………...17 圖3.3 Control組在顯微鏡下觀察到的影像……………………………………...18 圖3.4 Doxil組在顯微鏡下觀察到的影像………………………………………...20 圖3.5 Doxil組在顯微鏡下觀察到的影像………………………………………..21 圖3.6 Doxil組在顯微鏡下觀察到的影像………………………………………..22 圖3.7 Doxil_MB US組在顯微鏡下觀察到的影像………………………………24 圖3.8 Doxil_MB US組在顯微鏡下觀察到的影像………………………………25 圖3.9 Doxil_MB US組在顯微鏡下觀察到的影像………………………………26 圖3.10 Doxil_MB US組在顯微鏡下觀察到的影像……………………………..27 圖3.11 HT組在顯微鏡下觀察到的影像…………………………………………29 圖3.12 HT組在顯微鏡下觀察到的影像…………………………………………30 圖3.13 HT組在顯微鏡下觀察到的影像…………………………………………31 圖3.14 Doxil_HT組在顯微鏡下觀察到的影像………………………………….33 圖3.15 Doxil_HT組在顯微鏡下觀察到的影像………………………………….34 圖3.16 Doxil_HT組在顯微鏡下觀察到的影像………………………………….35 圖3.17 Doxil_HT組在顯微鏡下觀察到的影像………………………………….36 圖3.18 Doxil_MB US_HT組在顯微鏡下觀察到的影像………………………..38 圖3.19 Doxil_MB US_HT組在顯微鏡下觀察到的影像………………………..39 圖3.20 Doxil_MB US_HT組在顯微鏡下觀察到的影像………………………..40 圖3.21 Doxil_MB US_HT組在顯微鏡下觀察到的影像………………………..41 圖3.22 Doxil_MB US_HT組在顯微鏡下觀察到的影像………………………..42 圖3.23 六組間治療前後的比較…………………………………………………..44 圖3.24 六組間治療前後的比較…………………………………………………..46 圖3.25 六組間治療前後的比較…………………………………………………..48 圖3.26 HT組、Doxil_HT組、Doxil_MB US_HT組的腫瘤體積大小與時間之關係圖………………………………………………………………………………..50 圖3.27 HT組、Doxil_HT組、Doxil_MB US_HT組的腫瘤體積成長百分比與時間之關係圖………………………………………………………………………..51 圖3.28 Doxil組、Doxil_MB US組、Doxil_HT組、Doxil_MB US_HT組的腫瘤體積大小與時間之關係圖………………………………………………………..52 圖3.29 Doxil組、Doxil_MB US組、Doxil_HT組、Doxil_MB US_HT組的腫瘤體積成長百分比與時間之關係圖………………………………………………..53 圖3.30 Doxil_MB US組、Doxil_HT組、Doxil_MB US_HT組的腫瘤體積大小與時間之關係圖……………………………………………………………………..54 圖3.31 Doxil_MB US組、Doxil_HT組、Doxil_MB US_HT組的腫瘤體積成長百分比與時間之關係圖……………………………………………………………..55 圖3.32 Control組、HT組、Doxil組、Doxil_MB US組、Doxil_ HT組、Doxil_MB US_HT組的腫瘤體積大小與時間之關係圖………………………………………56 圖3.33 Control組、HT組、Doxil組、Doxil_MB US組、Doxil_ HT組、Doxil_MB US_HT組的腫瘤體積大小取Log與時間之semiLog關係圖……………………57 圖3.34 Control組、HT組、Doxil組、Doxil_MB US組、Doxil_ HT組、Doxil_MB US_HT組的腫瘤體積成長百分比取Log與時間之semiLog關係圖……………58 | |
| dc.language.iso | zh-TW | |
| dc.subject | 微氣泡 | zh_TW |
| dc.subject | 超音波 | zh_TW |
| dc.subject | 小鼠耳朵腫瘤模型 | zh_TW |
| dc.subject | 熱治療 | zh_TW |
| dc.subject | 藥物傳遞 | zh_TW |
| dc.subject | hyperthermia | en |
| dc.subject | mouse ear tumor model | en |
| dc.subject | drug delivery | en |
| dc.subject | microbubbles | en |
| dc.subject | ultrasound | en |
| dc.title | 奈米抗癌藥物結合脈衝超音波及微氣泡與熱治療在小鼠耳朵腫瘤之研究 | zh_TW |
| dc.title | Nanodrug Combined with Physical Methods for Cancer Tumor Treatment in A Mouse Ear Model | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 98-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 林峰輝,張富雄,謝銘鈞 | |
| dc.subject.keyword | 超音波,微氣泡,熱治療,藥物傳遞,小鼠耳朵腫瘤模型, | zh_TW |
| dc.subject.keyword | ultrasound,microbubbles,hyperthermia,drug delivery,mouse ear tumor model, | en |
| dc.relation.page | 66 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2010-08-05 | |
| dc.contributor.author-college | 工學院 | zh_TW |
| dc.contributor.author-dept | 醫學工程學研究所 | zh_TW |
| 顯示於系所單位： | 醫學工程學研究所 | |
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