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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 董成淵 | |
dc.contributor.author | Yu-Yang Lee | en |
dc.contributor.author | 李聿揚 | zh_TW |
dc.date.accessioned | 2021-06-15T04:29:31Z | - |
dc.date.available | 2019-08-20 | |
dc.date.copyright | 2009-08-21 | |
dc.date.issued | 2009 | |
dc.date.submitted | 2009-08-19 | |
dc.identifier.citation | 1. So PTC, Dong CY, Masters BR, Berland KM. Two-photon excitation fluorescence microscopy. Annual Review of Biomedical Engineering 2000;2:399-429.
2. Shankar R. Principles of quantum mechanics. Plenum Press, 1994. 3. Boyd RW. Nonlinear optics. Academic, 2008. 4. Liu Y, Chen HC, Yang SM, Sun TL, Lo W, Chiou LL, Huang GT, Dong CY, Lee HS. Visualization of hepatobiliary excretory function by intravital multiphoton microscopy. Journal of Biomedical Optics 2007;12:-. 5. Li FC, Liu Y, Huang GT, Chiou LL, Liang JH, Sun TL, Dong CY, Lee HS. In vivo dynamic metabolic imaging of obstructive cholestasis in mice. American Journal of Physiology-Gastrointestinal and Liver Physiology 2009;296:G1091-G1097. 6. Moore KL, Dalley AF, Agur AMR. Clinically oriented anatomy. Lippincott Williams & Wilkins, 2009. 7. Junqueira LCU, Carneiro J, Kelley RO. Basic histology. Appleton & Lange, 1992. 8. Ross MH, Pawlina W. Histology : a text and atlas : with correlated cell and molecular biology. Lippincott Wiliams & Wilkins, 2006. 9. Young B, Wheater PR. Wheater's functional histology : a text and colour atlas. Churchill Livingstone, 2006. 10. Jungermann K, Kietzmann T. Zonation of parenchymal and nonparenchymal metabolism in liver. Annual Review of Nutrition 1996;16:179-203. 11. Friedman SL. Mechanisms of hepatic fibrogenesis. Gastroenterology 2008;134:1655-1669. 12. Breeuwer P, Drocourt JL, Bunschoten N, Zwietering MH, Rombouts FM, Abee T. Characterization of Uptake and Hydrolysis of Fluorescein Diacetate and Carboxyfluorescein Diacetate by Intracellular Esterases in Saccharomyces-Cerevisiae, Which Result in Accumulation of Fluorescent Product. Applied and Environmental Microbiology 1995;61:1614-1619. 13. Weiss TF. Cellular biophysics. MIT Press, 1996. 14. Born M, Wolf E. Principles of optics : electromagnetic theory of propagation, interference and diffraction of light. Cambridge University Press, 1999. 15. Goodall H, Johnson MH. Use of Carboxyfluorescein Diacetate to Study Formation of Permeable Channels between Mouse Blastomeres. Nature 1982;295:524-526. 16. Kalinichenko VV, Bhattacharyya D, Zhou Y, Gusarova GA, Kim W, Sin B, Costa RH. Foxf1 +/- mice exhibit defective stellate cell activation and abnormal liver regeneration following CCl4 injury. Hepatology 2003;37:107-117. 17. Aleksunes LM, Scheffer GL, Jakowski AB, Pruimboom-Brees IM, Manautou JE. Coordinated expression of multidrug resistance-associated proteins (Mrps) in mouse liver during toxicant-induced injury. Toxicological Sciences 2006;89:370-379. 18. Aleksunes LM, Slitt AM, Cherrington NJ, Thibodeau MS, Klaassen CD, Manautou JE. Differential expression of mouse hepatic transporter genes in response to acetaminophen and carbon tetrachloride. Toxicological Sciences 2005;83:44-52. 19. Yoshiji H, Kuriyama S, Yoshii J, Ikenaka Y, Noguchi R, Nakatani T, Tsujinoue H, Yanase K, Namisaki T, Imazu H, Fukui H. Tissue inhibitor of metalloproteinases-1 attenuates spontaneous liver fibrosis resolution in the Transgenic mouse. Hepatology 2002;36:850-860. 20. Portincasa P, Palasciano G, Svelto M, Calamita G. Aquaporins in the hepatobiliary tract. Which, where and what they do in health and disease. Eur J Clin Invest 2008;38:1-10. 21. Rojek AM, Skowronski MT, Fuchtbauer EM, Fuchtbauer AC, Fenton RA, Agre P, Frokiaer J, Nielsen S. Defective glycerol metabolism in aquaporin 9 (AQP9) knockout mice. Proc Natl Acad Sci U S A 2007;104:3609-14. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/45598 | - |
dc.description.abstract | 肝乃是人體最重要的器官之一,而慢性乾病變長年在亞洲位居十大死因之榜上,因此對於肝臟的研究刻不容緩。然而肝是沈默的器官,缺乏神經來表達它的狀態,只能透過外在的觀察來判斷肝的狀況。現行的檢查技術,例如:GPT、GOT、ICG、CT、MRI…等等,固然已經幫人類解決很多問題了,但其共同點為:缺乏一個活體顯微以及動態及時的觀測。
利用雙光子螢光顯微鏡以及肝視窗,我們可以看見動態螢光分子(6-CFDA),在肝臟中的即時動態。而解析度可以進一步的到達細胞(微米)等級。同時我們發現,肝臟代謝6-CFDA的過程,可以藉由兩個第一階速率方程(first-order rate equation)來描述。利用微分方程的解,來模擬螢光變化,我們以此量化肝臟攝取(k1)以及排出(k2)6-CFDA的能力。並藉由比較此二參數,來定義乾細胞的正常與否,或者整體肝臟的狀態。同時探討參數的分布狀況,可以看出肝小葉中細胞能力的分布,並討論其原因。 最終的目的在於:希望可以藉由直接觀察描述顯微活體影像,來連結其他方法所觀測到的巨觀病理狀況。 | zh_TW |
dc.description.abstract | Liver is one of the most important organs in our bodies. Meanwhile, liver disease is one of the leading causes of death in Asia, especially Taiwan. Study of liver is important and urgent. Unfortunately, liver is one of the organs lacking neuro system to report its situation. Diagnoses of liver, such as GPT, GOT, ICG, CT, MRI…etc. already solved lots of problem for human being. One of their common drawback is they don’t have real-time and in vivo microscopic analysis.
Utilizing two-photon fluorescent microscopy (TPFM) and liver chamber. We would able to monitor real-time in vivo image of movement of fluorescent probe, 6-CFDA. At the same time, we found that the metabolism of 6-CFDA could be expressed by two first-order rate equations. These equations helped us to quantify ability of influx (k1) and efflux (k2) of hepatocytes. By comparing these for parameters, we’d like to define cells’ situations or stages of whole liver. Meanwhile, zonation difference could be revealed by discussion of distribution of both parameters. Finding the connection between macroscopic pathology and histology in previous studies and in vivo microscopic situation we monitored is our ultimate purpose. | en |
dc.description.provenance | Made available in DSpace on 2021-06-15T04:29:31Z (GMT). No. of bitstreams: 1 ntu-98-R96222052-1.pdf: 12611580 bytes, checksum: 6d228cb346f7e0cf65f8ca7c56ba2861 (MD5) Previous issue date: 2009 | en |
dc.description.tableofcontents | Abstract I
中文摘要 II Table of Content III Table of Figure IV Catalogue of Tables V CHAPTER 1 Introduction 1 CHAPTER 2 Histology 4 2.1 Normal Liver 4 2.2 Several Types of Functional Cells in Liver 10 CHAPTER 3 Basic Principles 13 3.1 Two-Photon Excitation 13 3.2 First-Order Rate Equations 16 3.3 Second Harmonic Generation 18 3.4 Principle of Microscope 21 CHAPTER 4 Material and Method 24 4.1 Laser and Microscope 24 4.2 Induction of Liver Fibrosis 25 4.3 Operation Procedures Before Observations 26 4.4 Fluorescent Probes 30 CHAPTER 5 Analysis and Result 32 5.1 Microscopic 32 5.2 Semi-Microscopic 44 CHAPTER 6 Conclusion and Discussion 47 6.1 Microscopic 47 6.2 Defining Recovery on semi microscopic scale 49 Reference 52 致謝 VII | |
dc.language.iso | en | |
dc.title | 利用雙光子顯微術以及動態模型觀測肝代謝功能 | zh_TW |
dc.title | Utilizing Multiphoton Microscopy and Kinetic Model to Monitor In Vivo Metabolic Activity of the Liver | en |
dc.type | Thesis | |
dc.date.schoolyear | 97-2 | |
dc.description.degree | 碩士 | |
dc.contributor.coadvisor | 李宣書 | |
dc.contributor.oralexamcommittee | 陳永芳,陳培哲 | |
dc.subject.keyword | 雙光子顯微鏡,二倍頻訊號,活體,肝臟代謝,肝纖維化,動態模型, | zh_TW |
dc.subject.keyword | two-photon fluorescent microscopy (TPFM),second harmonic generation (SHG),in vivo,hepatic metabolism,liver fibrosis,kinetic model, | en |
dc.relation.page | 54 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2009-08-20 | |
dc.contributor.author-college | 理學院 | zh_TW |
dc.contributor.author-dept | 物理研究所 | zh_TW |
顯示於系所單位: | 物理學系 |
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