電致性蛋白質塗佈技術與理論分析

Abstract

蛋白質塗佈是將蛋白質或生物分子固定在特定空間上，此技術是生物感測晶片發展的基礎，利用微奈米製程技術，可以方便製作出微奈米尺寸的元件。塗佈的目標物可為蛋白質、DNA等各種生物分子。蛋白質塗佈可應用的範圍極廣，包過基因工程、癌症研究、藥物研發、細胞生長、組織工程等等。 現有的蛋白質塗佈技術中，很難同時兼顧解析度並避開在蛋白質塗佈中直接對蛋白質造成的物理性或化學性傷害。為了克服此問題，本研究利用電致性的概念，提供了快速且高解析度的蛋白質塗佈技術。同時也不會造成蛋白質結構變型，以保持其活性，提高其應用之可行性。

Bio-microsystem creates the opportunity of on-chip screening, biodetection, and cell monitoring for emerging bio-diagnostic applications and innovative bio-scientific discovery. One of the fundamental techniques to achieve these bio-devices and bio-microsystem is the protein patterning. Traditionally, this is achieved by microfabrication techniques such as photolithography technology, inkjet printing and micro-contact printing. However, most of the existing patterning methods require complicated processes, and hardly achieve submicron protein patterns. In this thesis, we have developed a protein patterning technique by controlling the surface electric potential. Besides, we also develop the statistical model to describe the protein patterning results. Based on these results, the insight understanding of the interaction between protein and the device surface has been established.