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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 楊恩誠(En-Cheng Yang) | |
dc.contributor.author | Hui-Chun Chang | en |
dc.contributor.author | 張惠君 | zh_TW |
dc.date.accessioned | 2021-06-15T04:15:13Z | - |
dc.date.available | 2010-01-21 | |
dc.date.copyright | 2010-01-21 | |
dc.date.issued | 2010 | |
dc.date.submitted | 2010-01-12 | |
dc.identifier.citation | 王重雄、羅竹芳、乃育昕、王志源、陳韻如、黃偉峰、簡慈盈、吳治宇。2009。蜂群衰竭失調症。台灣昆蟲 29: 119-138。
安奎、何鎧光、陳裕文。2004。養蜂學。國立編譯館,台北,524頁。 Abramson, C.I., I.S. Aquino, F.S. Ramalho, and J.M. Price. 1999. The effect of insecticides on learning in the Africanized honey bee (Apis Mellifera L.). Arch. Environ. Contam. Toxicol. 37: 529-535. Bai, D., S. C. R. Lummis, W. Leicht, H. Breer, and D. B. Sattelle. 1991. Actions of imidacloprid and a related nitromethylene on cholinergic receptors of identified insect motor neurone. Pestic. Sci. 33: 197-204. Bicker, G. 1999. Histochemistry of classical neurotransmitters in antennal lobes and mushroom bodies of the honeybee. Microsc. Res. Tech. 45:174-183. Bicker, G., and S. Kreissl. 1994. Calcium imaging reveals nicotinic acetylcholine receptors on cultured mushroom body neurons. J. Neurophysiol. 71:808–810. Blanchard, P., F. Schurr, O. Celle, N. Cougoule, P. Drajnudel, R. Thiéry, J.-P. Faucon, and M. Ribière. 2008. First detection of Israeli acute paralysis virus (IAPV) in France, a dicistrovirus affecting honeybees (Apis mellifera). J. Invertebr. 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dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/45343 | - |
dc.description.abstract | 益達胺為接觸性與胃毒性之系統性殺蟲劑,可藉由葉面噴灑、粒劑灑佈、澆灌或種子處理等方式來保護植物不受害蟲侵襲,但施用同時也對蜜蜂造成很大的影響。不僅外勤蜂易接觸含有益達胺的植物,同時也會將含有益達胺的花蜜及花粉帶回巢房儲存;而內勤蜂則將含有益達胺的花蜜及花粉餵食幼蟲。前人研究指出,餵食蜂群含有低劑量益達胺的蔗糖溶液並不會影響幼蟲的封蓋率,但對於封蓋後的幼蟲是否能夠成功化蛹及羽化則未進一步的探討。由上述所知,低劑量的益達胺不至於造成急性中毒症狀,但幼蟲在生長期間體內所累積的益達胺可能會影響幼蟲的發育。本研究藉由給予蜜蜂幼蟲0.4 ~ 8000 ng 益達胺及其溶劑 0.1、1% DMSO 的水溶液,以觀察該藥劑是否對幼蟲封蓋、化蛹與成蟲羽化造成影響。益達胺劑量為2000 ~ 8000 ng 時,幼蟲的封蓋、化蛹及羽化率皆明顯降低。然而 0.1、1% DMSO及 0.4 ~ 200 ng對幼蟲的封蓋、化蛹及羽化率皆無顯著差異。雖然益達胺的亞致死劑量並未影響羽化率,將羽化後的工蜂進行口吻延伸反應 (proboscis extension reflex, PER) 之測試以評估其學習能力,結果發現給予幼蟲 0.04 ng以上的益達胺,羽化後的學習能力明顯降低,推測低劑量的益達胺可能影響蜜蜂的神經系統發育,進而造成工蜂喪失其訪花採蜜的功能。 | zh_TW |
dc.description.abstract | Imidacloprid is one of systemic insecticides. The residue of imidacloprid in the nectar and pollens of the plants would be poisonous not only to the honeybee adults but also larvae. It has been shown that the development of the honeybee larvae were impeded if the larvae were fed with imidacloprid contaminated nectar or pollen collected by bee workers. However, our understanding on the risk of imidacloprid to honeybee larvae is still in a primitive stage. In this study, the survival, capped-brood, pupation and eclosion rates were recorded after treating the larvae directly in the hive with imidacloprid in different dosages. The larvae brood-capped rates significantly decreased when the dosages increased from 2000 to 8000 ng, but no significant effect on the survival, brood-capped, pupation and eclosion rates when treated with DMSO or 0.4-200 ng imidacloprid. Although the sub-lethal dosage of imidacloprid had no effect on the eclosion rate, the learning ability of the poisoned bees tested with the classical olfactory conditioning, proboscis extension reflex. The result showed the larvae treated with 0.04 ng imidacloprid, the learning ability of the poisoned bees were impaired. The result suggests that the sub-lethal dosage of imidacloprid damage the development of nervous system and the foraging ability. | en |
dc.description.provenance | Made available in DSpace on 2021-06-15T04:15:13Z (GMT). No. of bitstreams: 1 ntu-99-R96632013-1.pdf: 924548 bytes, checksum: e45c302d1c4edb11094e1995540914be (MD5) Previous issue date: 2010 | en |
dc.description.tableofcontents | 目錄
中文摘要...........................................i 英文摘要...........................................ii 目錄............................................iii 前言..............................1 前人研究.........................4 一、類尼古丁殺蟲劑的特性................4 二、益達胺的應用....................................5 三、益達胺對蜜蜂的毒性..................................5 1. 益達胺對蜜蜂記憶與學習能力的影響.........6 2. 益達胺對蜜蜂取食行為 (Foraging behavior) 的影響....6 3. 益達胺對蜜蜂活動性的影響............................7 4. 益達胺對蜜蜂歸巢比例的影響......................7 5. 益達胺對蜂群的影響.........................8 材料與方法.................................10 一、田間蜂群幼蟲試驗............10 1. 供試蟲源...............................10 2. 藥劑配製...........................10 3. 試驗流程........................11 4. 統計分析..........................11 5. 半影響劑量 (median effective dose, ED50) 的計算..11 二、羽化後學習能力之測試 1. 供試蟲源..........11 2. 學習能力之測試...........................12 3. PER反應比例之計算公式.....................13 4. 統計分析................................13 結果.......................14 一、田間蜂群幼蟲試驗........................14 1. DMSO 對蜜蜂幼蟲的影響........................14 2. 益達胺對幼蟲封蓋率的影響.............14 3. 益達胺對幼蟲化蛹率的影響.....................14 4. 益達胺對幼蟲羽化率的影響........................15 二、羽化後的學習行為...................15 1. DMSO 對蜜蜂學習能力之影響.............15 2. 益達胺對蜜蜂學習能力之影響...................15 討論...............................17 ㄧ、 有機溶劑對蜜蜂的影響..........................17 二、 益達胺對蜜蜂幼蟲的影響...........................17 三、 益達胺對蜜蜂學習能力的影響.................18 四、 蜜蜂衰竭失調症........................20 參考文獻.............................21 圖.................................35 附錄...................................43 | |
dc.language.iso | zh-TW | |
dc.title | 益達胺的亞致死劑量對蜜蜂學習行為之影響 | zh_TW |
dc.title | Abnormality of honeybee learning behavior caused by sub-lethal dosage of imidacloprid | en |
dc.type | Thesis | |
dc.date.schoolyear | 98-1 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 何鎧光(Kai-kwong Ho),陳裕文(Yu-Wen Chen) | |
dc.subject.keyword | 益達胺,蜜蜂,幼蟲,口吻延伸反應, | zh_TW |
dc.subject.keyword | Imidacloprid,honeybee,larva,proboscis extension reflex, | en |
dc.relation.page | 44 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2010-01-13 | |
dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
dc.contributor.author-dept | 昆蟲學研究所 | zh_TW |
顯示於系所單位: | 昆蟲學系 |
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