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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 周崇熙(Chung-Hsi JiuJiu Chou) | |
dc.contributor.author | Chia-Yuan Chang | en |
dc.contributor.author | 張嘉元 | zh_TW |
dc.date.accessioned | 2021-06-15T04:07:06Z | - |
dc.date.available | 2010-02-11 | |
dc.date.copyright | 2010-02-11 | |
dc.date.issued | 2010 | |
dc.date.submitted | 2010-02-08 | |
dc.identifier.citation | 邱乾順,吳和生,2007,食因性細菌傳染分子分型監測網(PulseNet)簡介。疫情報導 23,204-221。
彭明興,2001,中華民國輸入犬貓食品之檢疫條件簡介。農政與農情, 112。 費昌勇,2006, 94 年度全國家犬家貓數目調查與六年分析摘要。http://bulletin.coa.gov.tw/files/news/9_diamond_20060109113038/001.doc。 廖智偉,王裕智,蔡宇馨,宣詩玲,葉光勝,張照勤,陳德勳,2008,臺灣中部地區流浪犬及家犬沙門氏菌之調查。臺灣獸醫誌 34,7。 Aarestrup, F.M., Hendriksen, R.S., Lockett, J., Gay, K., Teates, K., McDermott, P.F., White, D.G., Hasman, H., Sorensen, G., Bangtrakulnonth, A., Pornreongwong, S., Pulsrikarn, C., Angulo, F.J., Gerner-Smidt, P., 2007, International spread of multidrug-resistant Salmonella Schwarzengrund in food products. Emerg Infect Dis 13, 726-731. Agustin, A.I., Carraminana, J.J., Rota, C., Herrera, A., 2005, Antimicrobial resistance of Salmonella spp. from pigs at slaughter in Spain in 1993 and 2001. Lett Appl Microbiol 41, 39-44. Andrews, W.H., Hammack, T.S., 2003, Salmonella,Chapter 5. FDA Bacteriological analytical manual. Anonymous 2000. Human health risk from exposure to natural dog treats. In Can Commun Dis Rep, pp. 41-42. Arun, K.B., 2008, Salmonella enterica. foodborne microbial pathogens: mechanisms and pathogens, 201-216. Bager, F., Helmuth, R., 2001, Epidemiology of resistance to quinolones in Salmonella. Vet Res 32, 285-290. Baucheron, S., Chaslus-Dancla, E., Cloeckaert, A., Chiu, C.H., Butaye, P., 2005, High-level resistance to fluoroquinolones linked to mutations in gyrA, parC, and parE in Salmonella enterica serovar Schwarzengrund isolates from humans in Taiwan. Antimicrob Agents Chemother 49, 862-863. Betancor, L., Schelotto, F., Martinez, A., Pereira, M., Algorta, G., Rodriguez, M.A., Vignoli, R., Chabalgoity, J.A., 2004, Random amplified polymorphic DNA and phenotyping analysis of Salmonella enterica serovar Enteritidis isolates collected from humans and poultry in Uruguay from 1995 to 2002. J Clin Microbiol 42, 1155-1162. Boscan-Duque, L.A., Arzalluz-Fisher, A.M., Ugarte, C., Sanchez, D., Wittum, T.E., Hoet, A.E., 2007, Reduced susceptibility to quinolones among Salmonella serotypes isolated from poultry at slaughter in Venezuela. J Food Prot 70, 2030-2035. Botteldoorn, N., Herman, L., Rijpens, N., Heyndrickx, M., 2004, Phenotypic and molecular typing of Salmonella strains reveals different contamination sources in two commercial pig slaughterhouses. Appl Environ Microbiol 70, 5305-5314. CDC, 2006, Human salmonellosis associated with animal-derived pet treats--United States and Canada, 2005. MMWR Morb Mortal Wkly Rep 55, 702-705. CDC, 2008a, Multistate outbreak of human Salmonella infections caused by contaminated dry dog food--United States, 2006-2007. MMWR Morb Mortal Wkly Rep 57, 521-524. CDC, 2008b, Preliminary FoodNet data on the incidence of infection with pathogens transmitted commonly through food--10 states, 2007. MMWR Morb Mortal Wkly Rep 57, 366-370. CDC, 2009a, Preliminary FoodNet Data on the incidence of infection with pathogens transmitted commonly through food--10 States, 2008. MMWR Morb Mortal Wkly Rep 58, 333-337. CDC 2009b. What is PulseNet? http://www.cdc.gov/pulsenet/whatis.htm. Chang, C.C., Lin, Y.H., Chang, C.F., Yeh, K.S., Chiu, C.H., Chu, C., Chien, M.S., Hsu, Y.M., Tsai, L.S., Chiou, C.S., 2005, Epidemiologic relationship between fluoroquinolone-resistant Salmonella enterica Serovar Choleraesuis strains isolated from humans and pigs in Taiwan (1997 to 2002). J Clin Microbiol 43, 2798-2804. Chengappa, M.M., Staats, J., Oberst, R.D., Gabbert, N.H., McVey, S., 1993, Prevalence of Salmonella in raw meat used in diets of racing greyhounds. J Vet Diagn Invest 5, 372-377. Cherry, B., Burns, A., Johnson, G.S., Pfeiffer, H., Dumas, N., Barrett, D., McDonough, P.L., Eidson, M., 2004, Salmonella Typhimurium outbreak associated with veterinary clinic. Emerg Infect Dis 10, 2249-2251. Clark, C., Cunningham, J., Ahmed, R., Woodward, D., Fonseca, K., Isaacs, S., Ellis, A., Anand, C., Ziebell, K., Muckle, A., Sockett, P., Rodgers, F., 2001, Characterization of Salmonella associated with pig ear dog treats in Canada. J Clin Microbiol 39, 3962-3968. Darwin, K.H., Miller, V.L., 1999, Molecular basis of the interaction of Salmonella with the intestinal mucosa. Clin Microbiol Rev 12, 405-428. Eriksson, J., Lofstrom, C., Aspan, A., Gunnarsson, A., Karlsson, I., Borch, E., de Jong, B., Radstrom, P., 2005, Comparison of genotyping methods by application to Salmonella livingstone strains associated with an outbreak of human salmonellosis. Int J Food Microbiol 104, 93-103. Finley, R., Reid-Smith, R., Ribble, C., Popa, M., Vandermeer, M., Aramini, J., 2008, The occurrence and anti-microbial susceptibility of Salmonellae isolated from commercially available pig ear pet treats. Zoonoses Public Health 55, 455-461. Finley, R., Reid-Smith, R., Weese, J.S., 2006, Human health implications of Salmonella-contaminated natural pet treats and raw pet food. Clin Infect Dis 42, 686-691. Finley, R., Ribble, C., Aramini, J., Vandermeer, M., Popa, M., Litman, M., Reid-Smith, R., 2007, The risk of salmonellae shedding by dogs fed Salmonella-contaminated commercial raw food diets. Can Vet J 48, 69-75. Frye, J.G., Fedorka-Cray, P.J., 2007, Prevalence, distribution and characterisation of ceftiofur resistance in Salmonella enterica isolated from animals in the USA from 1999 to 2003. Int J Antimicrob Agents 30, 134-142. Galan, J.E., Collmer, A., 1999, Type III secretion machines: bacterial devices for protein delivery into host cells. Science 284, 1322-1328. Gautom, R.K., 1997, Rapid pulsed-field gel electrophoresis protocol for typing of Escherichia coli O157:H7 and other gram-negative organisms in 1 day. J Clin Microbiol 35, 2977-2980. Giraud, E., Brisabois, A., Martel, J.L., Chaslus-Dancla, E., 1999, Comparative studies of mutations in animal isolates and experimental in vitro- and in vivo-selected mutants of Salmonella spp. suggest a counterselection of highly fluoroquinolone-resistant strains in the field. Antimicrob Agents Chemother 43, 2131-2137. Giraud, E., Cloeckaert, A., Baucheron, S., Mouline, C., Chaslus-Dancla, E., 2003, Fitness cost of fluoroquinolone resistance in Salmonella enterica serovar Typhimurium. J Med Microbiol 52, 697-703. Giraud, E., Cloeckaert, A., Kerboeuf, D., Chaslus-Dancla, E., 2000, Evidence for active efflux as the primary mechanism of resistance to ciprofloxacin in Salmonella enterica serovar Typhimurium. Antimicrob Agents Chemother 44, 1223-1228. Gurakan, G.C., Aksoy, C., Ogel, Z.B., Oren, N.G., 2008, Differentiation of Salmonella Typhimurium from Salmonella Enteritidis and other Salmonella serotypes using random amplified polymorphic DNA analysis. Poult Sci 87, 1068-1074. Helmuth, R., 2000, antibiotic resistance in Salmonella. CABI Publishing, Oxon, United Kingdom., p. 89-106 pp. Herschleb, J., Ananiev, G., Schwartz, D.C., 2007, Pulsed-field gel electrophoresis. Nat Protoc 2, 677-684. Hilton, A.C., Banks, J.G., Penn, C.W., 1996, Random amplification of polymorphic DNA (RAPD) of Salmonella: strain differentiation and characterization of amplified sequences. J Appl Bacteriol 81, 575-584. Hudson, C.R., Garcia, M., Gast, R.K., Maurer, J.J., 2001, Determination of close genetic relatedness of the major Salmonella Enteritidis phage types by pulsed-field gel electrophoresis and DNA sequence analysis of several Salmonella virulence genes. Avian Dis 45, 875-886. ISO, 2002, EN ISO 6579:2002. Microbiology of food and animal feeding stuffs-Horizontal method for the detection of Salmonella spp. Kiessling, C.R., Jackson, M., Watts, K.A., Loftis, M.H., Kiessling, W.M., Buen, M.B., Laster, E.W., Sofos, J.N., 2007, Antimicrobial susceptibility of Salmonella isolated from various products, from 1999 to 2003. J Food Prot 70, 1334-1338. Kozak, M., Horosova, K., Lasanda, V., Bilek, J., Kyselova, J., 2003, Do dogs and cats present a risk of transmission of salmonellosis to humans? Bratisl Lek Listy 104, 323-328. Laconcha, I., Lopez-Molina, N., Rementeria, A., Audicana, A., Perales, I., Garaizar, J., 1998, Phage typing combined with pulsed-field gel electrophoresis and random amplified polymorphic DNA increases discrimination in the epidemiological analysis of Salmonella Enteritidis strains. Int J Food Microbiol 40, 27-34. Lefebvre, S.L., Reid-Smith, R., Boerlin, P., Weese, J.S., 2008, Evaluation of the risks of shedding Salmonellae and other potential pathogens by therapy dogs fed raw diets in Ontario and Alberta. Zoonoses Public Health 55, 470-480. Lin, A.W., Usera, M.A., Barrett, T.J., Goldsby, R.A., 1996, Application of random amplified polymorphic DNA analysis to differentiate strains of Salmonella Enteritidis. J Clin Microbiol 34, 870-876. Lofstrom, C., Eriksson, J., Aspan, A., Haggblom, P., Gunnarsson, A., Borch, E., Radstrom, P., 2006, Improvement and validation of RAPD in combination with PFGE analysis of Salmonella enterica ssp enterica serovar Senftenberg strains isolated from feed mills. Veterinary Microbiology 114, 345-351. MacPherson, J.M., Eckstein, P.E., Scoles, G.J., Gajadhar, A.A., 1993, Variability of the random amplified polymorphic DNA assay among thermal cyclers, and effects of primer and DNA concentration. Mol Cell Probes 7, 293-299. Millemann, Y., Lesage, M.C., Chaslus-Dancla, E., Lafont, J.P., 1995, Value of plasmid profiling, ribotyping, and detection of IS200 for tracing avian isolates of Salmonella typhimurium and S. enteritidis. J Clin Microbiol 33, 173-179. Molbak, K., Gerner-Smidt, P., Wegener, H.C., 2002, Increasing quinolone resistance in Salmonella enterica serotype Enteritidis. Emerg Infect Dis 8, 514-515. OIE 2008. Other diseases: Salmonellosis. In: Manual of diagnostic tests and vaccines for terrestrial animals., p. 1268. Olive, D.M., Bean, P., 1999, Principles and applications of methods for DNA-based typing of microbial organisms. J Clin Microbiol 37, 1661-1669. Piddock, L.J., 2002, Fluoroquinolone resistance in Salmonella serovars isolated from humans and food animals. FEMS Microbiol Rev 26, 3-16. Piddock, L.J., White, D.G., Gensberg, K., Pumbwe, L., Griggs, D.J., 2000, Evidence for an efflux pump mediating multiple antibiotic resistance in Salmonella enterica serovar Typhimurium. Antimicrob Agents Chemother 44, 3118-3121. Pitout, J.D., Reisbig, M.D., Mulvey, M., Chui, L., Louie, M., Crowe, L., Church, D.L., Elsayed, S., Gregson, D., Ahmed, R., Tilley, P., Hanson, N.D., 2003, Association between handling of pet treats and infection with Salmonella enterica serotype Newport expressing the AmpC beta-lactamase, CMY-2. J Clin Microbiol 41, 4578-4582. Randall, L.P., Bagnall, M.C., Karatzas, K.A., Coldham, N.C., Piddock, L.J., Woodward, M.J., 2008, Fitness and dissemination of disinfectant-selected multiple-antibiotic-resistant (MAR) strains of Salmonella enterica serovar Typhimurium in chickens. J Antimicrob Chemother 61, 156-162. Randall, L.P., Eaves, D.J., Cooles, S.W., Ricci, V., Buckley, A., Woodward, M.J., Piddock, L.J., 2005, Fluoroquinolone treatment of experimental Salmonella enterica serovar Typhimurium DT104 infections in chickens selects for both gyrA mutations and changes in efflux pump gene expression. J Antimicrob Chemother 56, 297-306. Schierwater, B., Ender, A., 1993, Different thermostable DNA polymerases may amplify different RAPD products. Nucleic Acids Res 21, 4647-4648. Schotte, U., Borchers, D., Wulff, C., Geue, L., 2007, Salmonella Montevideo outbreak in military kennel dogs caused by contaminated commercial feed, which was only recognized through monitoring. Vet Microbiol 119, 316-323. Seepersadsingh, N., Adesiyun, A.A., Seebaransingh, R., 2004, Prevalence and antimicrobial resistance of Salmonella spp. in non-diarrhoeic dogs in Trinidad. J Vet Med B Infect Dis Vet Public Health 51, 337-342. Soto, S.M., Gonzalez-Hevia, M.A., Mendoza, M.C., 2003, Antimicrobial resistance in clinical isolates of Salmonella enterica serotype Enteritidis: relationships between mutations conferring quinolone resistance, integrons, plasmids and genetic types. J Antimicrob Chemother 51, 1287-1291. Strohmeyer, R.A., Morley, P.S., Hyatt, D.R., Dargatz, D.A., Scorza, A.V., Lappin, M.R., 2006, Evaluation of bacterial and protozoal contamination of commercially available raw meat diets for dogs. J Am Vet Med Assoc 228, 537-542. Tai, P.C., Kessler, D.P., Ingraham, J., 1969, Cold-sensitive mutations in Salmonella typhimurium which affect ribosome synthesis. J Bacteriol 97, 1298-1304. Tauni, M.A., Osterlund, A., 2000, Outbreak of Salmonella Typhimurium in cats and humans associated with infection in wild birds. J Small Anim Pract 41, 339-341. Tenover, F.C., Arbeit, R.D., Goering, R.V., Mickelsen, P.A., Murray, B.E., Persing, D.H., Swaminathan, B., 1995, Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis: criteria for bacterial strain typing. J Clin Microbiol 33, 2233-2239. Tindall, B.J., Grimont, P.A., Garrity, G.M., Euzeby, J.P., 2005, Nomenclature and taxonomy of the genus Salmonella. Int J Syst Evol Microbiol 55, 521-524. Tsai, H.J., Huang, H.C., Lin, C.M., Lien, Y.Y., Chou, C.H., 2007, Salmonellae and campylobacters in household and stray dogs in northern Taiwan. Vet Res Commun 31, 931-939. Tsen, H.Y., Lin, J.S., Hu, H.H., Liu, P.R., Wang, T.K., 1999, Use of pulsed field gel electrophoresis as an epidemiological tool for analysis of sporadic associated strains of Salmonella Typhi isolated in Taiwan. J Appl Microbiol 86, 761-768. van den Bogaard, A.E., Stobberingh, E.E., 2000, Epidemiology of resistance to antibiotics. Links between animals and humans. Int J Antimicrob Agents 14, 327-335. Van Immerseel, F., Pasmans, F., De Buck, J., Rychlik, I., Hradecka, H., Collard, J.M., Wildemauwe, C., Heyndrickx, M., Ducatelle, R., Haesebrouck, F., 2004, Cats as a risk for transmission of antimicrobial drug-resistant Salmonella. Emerg Infect Dis 10, 2169-2174. Webber, M.A., Piddock, L.J., 2003, The importance of efflux pumps in bacterial antibiotic resistance. J Antimicrob Chemother 51, 9-11. Weese, J.S., Rousseau, J., Arroyo, L., 2005, Bacteriological evaluation of commercial canine and feline raw diets. Can Vet J 46, 513-516. Welsh, J., McClelland, M., 1990, Fingerprinting genomes using PCR with arbitrary primers. Nucleic Acids Res 18, 7213-7218. White, D.G., Datta, A., McDermott, P., Friedman, S., Qaiyumi, S., Ayers, S., English, L., McDermott, S., Wagner, D.D., Zhao, S., 2003, Antimicrobial susceptibility and genetic relatedness of Salmonella serovars isolated from animal-derived dog treats in the USA. J Antimicrob Chemother 52, 860-863. Williams, J.G., Kubelik, A.R., Livak, K.J., Rafalski, J.A., Tingey, S.V., 1990, DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res 18, 6531-6535. Willis, C., 2001, Isolation of Salmonella species from imported dog chews. Vet Rec 149, 426-427. Wong, T.L., Thom, K., Nicol, C., Heffernan, H., MacDiarmid, S., 2007, Salmonella serotypes isolated from pet chews in New Zealand. J Appl Microbiol 103, 803-810. Zheng, J., Keys, C.E., Zhao, S., Meng, J., Brown, E.W., 2007, Enhanced subtyping scheme for Salmonella Enteritidis. Emerg Infect Dis 13, 1932-1935. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/45165 | - |
dc.description.abstract | 感染沙門氏菌的犬、貓皆可能經由糞便排菌而直接或間接感染人類,且有許多人類沙門氏菌症案例肇因於接觸犬貓或其食品。臺灣至今並無任何犬貓食品中沙門氏菌之調查資料。本調查於2008年收集全臺灣各地共413個犬貓食品樣本(犬食品276個樣本,貓食品137個樣本),包括本地製造或國外進口之一般飼料、處方飼料、罐頭和零食等。調查結果共從其中11個樣本分離出13株沙門氏菌,總分離率為2.7% (11/413),而不同種類或不同製造地的食品間並無統計上差異。經血清學鑑定,Salmonella Schwarzengrund(7/13, 53.9%)為最常被分離出的血清型,其次為S. Enteritidis (4/13, 30.8%)。使用藥片擴散法進行14種抗菌劑之感受性試驗,結果有93% 的分離株對一種或一種以上的抗菌劑有抗藥性,其中對streptomycin (84.6%) 抗藥性比率最高,而對cefoxitin、ceftriaxone、ciprofloxacin、gentamicin及sulphamethoxazole/trimethoprim五種抗菌劑均有100%感受性。在分子生物學上,使用脈衝式電泳(pulsed field gel electrophoresis, PFGE)及隨機擴增多型性DNA(Random Amplified polymorphic DNA, RAPD)判斷各分離株間的親緣關係,PFGE使用六種酵素,僅一個酵素PacI 可辨識一分離株S. Schwarzengrund與其同血清型分離株間的不同,其他的酵素均無法區分出同血清型各分離株的不同,於是使用RAPD做為輔助工具,但分型效果仍不佳,在使用五種引子時,僅分離株S. Schwarzengrund使用引子P1254時可分出兩種不同的圖譜,其他引子均無法分出同血清型各分離株間的相異。本調查結果指出臺灣地區市售寵物食品中存在對人可能有致病性的沙門氏菌,一般飼主在間接或直接接觸寵物食品時有暴露於病原的風險。 | zh_TW |
dc.description.abstract | Salmonella-infected cats or dogs can shed the bacteria via feces which eventually causes human-salmonellosis. Several outbreaks worldwide have indicated that contact between human and dogs, cats, or pet foods may cause salmonellosis. There are no published surveys regarding Salmonella isolation from pet foods in Taiwan. In order to understand Salmonella contamination in pet foods and the antimicrobial susceptibility of the isolates, 413 pet food samples were collected, including 276 dog food and 137 cat food. These samples included normal dry feed, prescription diet, canned feed, and treats; the origins of these samples were sorted as locally produced or imported. The Salmonella isolation rate was 2.7% (11/413) from 11 samples with a total of 13 isolates found; no significant differences existed between any categories. The isolates were serotyped and S. Schwarzengrund was identified as the most prevalent serovar (53.9%), followed by S. Enteritidis (30.8%). Antimicrobial susceptibility of the isolates was determined via disk diffusion method. Ninety-three percent of the isolates were shown to have resistance to one or more antimicrobial agents, with the highest resistance rate to streptomycin (84.6%). The phylogenetic analysis was identified by pulsed field gel electrophoresis (PFGE) and random amplification of polymorphic DNA (RAPD) as an auxiliary tool. The isolates were indistinguishable by PFGE using six enzymes except one different S. Schwarzengrund isolate pattern. Only two different patterns of S. Schwarzengrund isolates by RAPD were found using primer P1254. The serotypes of the isolates from the pet food in Taiwan are potentially pathogenic to both humans and pets. Pets’ owners are at risk of exposure either by direct or indirect contact. | en |
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dc.description.tableofcontents | 中文摘要………………………………………………………………….I
Abstract ………………………………………………………………….II 目次……….…………………………………………………………….IV 表次……………………………………………………………….….VIII 圖次…………………………………………………………………IX 第一章 緒言…………………………………………………………….1 第二章 文獻探討……………………………………………………….4 第一節 沙門氏菌………………………………………………4 2.1.1沙門氏菌之特性與分類………………………………….4 2.1.2沙門氏菌之病原性………………………………………6 第二節 沙門氏菌之分離法………………………………………..8 第三節 寵物及人與沙門氏菌之相關性…………….………...….10 第四節 寵物食品與沙門氏菌之相關性………………………….12 第五節 沙門氏菌之藥物敏感性試驗…………………………….15 2.5.1沙門氏菌抗藥性機制……………………………………15 2.5.2沙門氏菌抗藥性的產生…………………………………17 2.5.3犬貓沙門氏菌抗藥性的流行病學……………...…….…20 2.5.4寵物食品中沙門氏菌抗藥性的研究調查………………21 第六節 分子生物學分型...……..…………………………………23 2.6.1脈衝式電泳……………………………………………23 2.6.2隨機擴增多型DNA…………………………………..25 2.6.3脈衝式電泳與隨機擴增多型性DNA於沙門氏菌之運用……………………….……………………………………27 第三章 材料與方法………………………….……………………..29 第一節 沙門氏菌之分離……………………....………………….29 3.1.1 樣本採集…………………....………………………….29 3.1.2 分離方法…………………....………………………….29 3.1.3 沙門氏菌血清型之鑑定…………....………………….30 3.1.4 培養基之配製……………....………………………….31 第二節 沙門氏菌之抗菌劑感受性試驗………………………….34 3.2.1 瓊脂藥片擴散法………………………………………34 3.2.2 配製瓊脂藥片擴散法所需之培養基…………………34 第三節 分離率統計………………………………………………35 第四節 分子生物學分型…………………………………………36 3.4.1 脈衝式電泳………….………………………………….36 3.4.1.1 菌塊準備……….………………………………...36 3.4.1.2 限制酶作用及脈衝式電泳之進行………………37 3.4.2 隨機擴增多型性DNA…………………………………….38 第四章 結果……………………………………………………………...40 第一節 沙門氏菌分離率…………………………………………....40 第二節 沙門氏菌血清型………………………………………….42 第三節 分離出沙門氏菌的寵物食品主要肉品成分及其他資料.43 第四節 沙門氏菌對抗菌劑感受性試驗………………………….44 第五節 分子生物學分型………………………………………….45 4.5.1 脈衝式電泳之分子分型……………………………….45 4.5.2 隨機擴增多型性DNA之分子分型……………………47 第五章 討論………………………………………….………………..49 第一節 寵物食品分離率討論…………………………………….49 第二節 分離株之血清型討論…………………………………….51 第三節 分離株之藥物性感受性試驗…………………………….54 第四節 分離株之分子生物學分型……………………………….56 第六章 結論……………………………………………………….61 第七章 參考文獻………………………………………………………63 表格……………………………………………………………………..69 圖片……………………………………………………………………..75 | |
dc.language.iso | zh-TW | |
dc.title | 臺灣地區犬貓飼料及零食沙門氏菌之分離鑑定分型暨抗菌劑感受性調查 | zh_TW |
dc.title | The Isolation Rate, Molecular Typing, and Antimicrobial Susceptibility of Salmonella from Dog- and Cat-Food Products in Taiwan | en |
dc.type | Thesis | |
dc.date.schoolyear | 98-1 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 蔡向榮,張紹光,連一洋,邱智賢 | |
dc.subject.keyword | 沙門氏菌,寵物食品,抗菌劑感受性試驗,脈衝式電泳,隨機擴增多型性DNA, | zh_TW |
dc.subject.keyword | Salmonella,pet food,antimicrobial susceptibility,PFGE,RAPD, | en |
dc.relation.page | 96 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2010-02-08 | |
dc.contributor.author-college | 獸醫專業學院 | zh_TW |
dc.contributor.author-dept | 獸醫學研究所 | zh_TW |
顯示於系所單位: | 獸醫學系 |
文件中的檔案:
檔案 | 大小 | 格式 | |
---|---|---|---|
ntu-99-1.pdf 目前未授權公開取用 | 8.01 MB | Adobe PDF |
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。