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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 黃榮山(Long-Sun Huang) | |
| dc.contributor.author | Po-Chun Tsai | en |
| dc.contributor.author | 蔡伯駿 | zh_TW |
| dc.date.accessioned | 2021-06-15T04:05:54Z | - |
| dc.date.available | 2010-02-11 | |
| dc.date.copyright | 2010-02-11 | |
| dc.date.issued | 2010 | |
| dc.date.submitted | 2010-02-08 | |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/45136 | - |
| dc.description.abstract | 幹細胞治療在心血管疾病的應用上漸漸受到關注，利用微機電製程製造的微晶片，提供比公分尺寸的實驗更接近人體的培養環境，例如細胞和細胞間的距離以及類似人體血管的培養體積，在這理論基礎下，微機電技術製造的微晶片提供流體剪應力的刺激，可以加速羊水幹細胞分化成內皮細胞
內皮細胞有沿著流場方向排列的特性，匯整人類羊水幹細胞在12 dyne/cm2 剪應力刺激24小時後的排列方向統計資料，剪應力刺激3小時後的排列角度分布和24小時的結果接近，表示其有相近的分化結果，此外，剪應力12 dyne/cm2 作用3小時以及24小時後，血小板與內皮細胞貼附分子(platelet endothelial cell adhesion molecule ,PECAM-1) 和 溫偉伯因子(von Willebrand Factor, vWF)都有明顯的表現，經過3小時的剪應力刺激，血小板與內皮細胞貼附分子, 溫偉伯因子以及血管內皮生長因子受體(vascular endothelial growth factor-2 receptor, VEGFR)的基因表現量都超過10倍以上，另外經過剪應力刺激後，在基質膠(matrigel) 基底上，觀察到低密度脂蛋白的堆積以及網狀結構形成，細胞在剪應力12 dyne/cm2 刺激下，分化程度比3, 6, and 18 dyne/cm2來的高 在巨觀尺寸的實驗架構下，需要數天才能達到分化的結果，而微流道系統成功的提供了快速分化的環境，人類羊水細胞在微流道系統的培養環境下，3小時就有明顯分化結果，本研究顯現出其在幹細胞的治療以及心血管疾病的研究上的潛力 | zh_TW |
| dc.description.abstract | Stem cell therapies for cardiovascular diseases are of great growing interest. Micro-electro-mechanism-system (MEMS) fabricated chips provide more in vivo like cell-to-cell distance and culturing volume than that in centimetre-scaled experiments. In this thesis, we are able to accelerate the process of endothelial differentiation of human amniotic fluid stem cells (hAFSCs) by the stimulation of fluidic shear stress in a MEMS-fabricated micro device.
Cells aligning to flow directions is a known property of endothelial cells. hAFSCs affected by 12 dyne/cm2 of shear stress were compiled statistics for the arrangement angles toward the flow direction in a 24-hour period. The angular distribution of cells after 3 hours of stress stimulation approaches to that of cells after 24 hours, indicating similar degrees of endothelial differentiation. Both cell samples stimulated by 12 dyne/cm2 stress after 3 hours and 24 hours exhibit protein expressions of platelet endothelial cell adhesion molecule (PECAM-1) and von Willebrand Factor (vWF). Over ten folds of gene expressions in PECAM-1, vWF and vascular endothelial growth factor-2 receptor (VEGFR) appeared after 3 hours stimulation. Uptakes of ac-LDL and formation of tube-like structure on Matrigel were also observed after shear stress stimulation. Cells stimulated by 12dyne/cm2 shear stress have higher degrees of differentiation than those stimulated by 3, 6, and 18 dyne/cm2 shear stress. Utilizing 3 hours to achieve differentiation instead of days in macro-scale experiments, this micro fluidic system has been proved successful for a rapid shear stress stimulation of hAFSCs differentiation into endothelial cells. This research shows its great potential in stem cell therapy and cardiovascular disease studies. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-15T04:05:54Z (GMT). No. of bitstreams: 1 ntu-99-R96543051-1.pdf: 5275540 bytes, checksum: ea53400f11008b2688edff1162c15f97 (MD5) Previous issue date: 2010 | en |
| dc.description.tableofcontents | 第一章 序論 1
1-1 前言 1 1-2 研究動機 3 1-3 文獻探討 5 1-4 研究方法 10 1-5 論文架構 11 第二章 實驗原理 12 2-1 細胞培養環境之控制 12 2-1-1 傳統細胞培養技術 12 2-1-2 微尺寸培養對細胞之影響 16 2-2 流體剪應力對細胞之影響與計算 19 2-3 流體剪應力對幹細胞分化為內皮細胞之影響 22 2-4 即時定量聚合酶連鎖反應 24 2-5 雷射共軛焦顯微鏡 25 2-6 血管生成與低密度脂蛋白攝取 26 第三章 製程設計與實驗方法 26 3-1 微流道晶片設計與製造 27 3-1-1 微流道晶片規格與設計 27 3-1-2 聚二甲基矽氧烷微流道製作 28 3-1-3 微流道晶片整合 29 3-2 實驗系統之架設 31 3-3 羊水幹細胞之培養 32 3-4 羊水幹細胞之剪應力刺激 33 第四章 實驗結果 35 4-1 羊水幹細胞受剪應力影響之排列效果 35 4-2 羊水幹胞受剪應力影響後之基因型表現 41 4-3 羊水幹胞受剪應力影響後之蛋白質表現 42 4-4 羊水幹細胞受剪應力影響後之血管生成實驗 46 第五章 結論以及未來展望 48 5-1 結論 48 5-2 未來展望 49 參考文獻 51 | |
| dc.language.iso | zh-TW | |
| dc.subject | 幹細胞 | zh_TW |
| dc.subject | 微流體 | zh_TW |
| dc.subject | 內皮細胞 | zh_TW |
| dc.subject | micro fluidics | en |
| dc.subject | stem cell | en |
| dc.subject | endothelial cells | en |
| dc.title | 微流體剪應力對羊水幹細胞分化為內皮細胞之影響 | zh_TW |
| dc.title | the effects of microfluidic shear stress on endothelial differentiation of amniotic fluid stem cells | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 98-1 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 黃效民(Shiaw-Min Hwang),王安邦(An-Bang Wang) | |
| dc.subject.keyword | 幹細胞,微流體,內皮細胞, | zh_TW |
| dc.subject.keyword | stem cell,micro fluidics,endothelial cells, | en |
| dc.relation.page | 53 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2010-02-09 | |
| dc.contributor.author-college | 工學院 | zh_TW |
| dc.contributor.author-dept | 應用力學研究所 | zh_TW |
| 顯示於系所單位： | 應用力學研究所 | |
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