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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 謝兆樞 | |
dc.contributor.author | Hsuan Chen | en |
dc.contributor.author | 陳玄 | zh_TW |
dc.date.accessioned | 2021-06-15T04:03:38Z | - |
dc.date.available | 2013-03-11 | |
dc.date.copyright | 2010-03-11 | |
dc.date.issued | 2010 | |
dc.date.submitted | 2010-02-11 | |
dc.identifier.citation | Abele, L.G., Kim, W., and Felgenhauer, B.E. (1989) . Molecular Evidence for Inclusion of the Phylum Pentastomida in the Crustacea. Mol Biol Evol 6, 685-691.
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dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/45078 | - |
dc.description.abstract | 大豆 (Glycine max (L.) Merr.) 是世界上最重要的豆科 (Leguminosae) 作物,大豆屬野生物種為育種上相當重要的遺傳資源。台灣是一年生野生大豆分佈之南界,同時也是多年生野生種大豆分佈之北界,其中包括四種基因組之野生物種,G. soja (GG 基因組)、G. tomentella (DDD1D1 基因組)、G. dolichocarpa (A6A6DD 基因組) 及G. tabacina (Tab074;A6A6B3B3 基因組) 。
大豆屬物種的染色體數量多 (2n=40),大小相似又缺乏明顯的形態特徵,對於其基因組內重複性序列的分佈位置及扮演角色的認識又相當缺乏,因此,大豆屬物種之基因組分類及染色體核型分析的研究都受到相當大的限制。利用螢光原位雜交 (fluorescent in situ hybridization, FISH) 技術可用於標定特定基因組或特定染色體區域,藉以了解物種基因組差異及並輔助染色體核型分析,解決分類爭議及染色體研究困難的問題。 本研究首先利用生物資訊的方式,於大豆全基因組定序 (whole-genome shotgun;WGS) 計畫資料庫內找到三個新的重複性序列,分別命名為SBRS1、SBRS2及SBRS3。SBRS1可做為大豆屬物種GG基因組之標幟。SBRS2、SBRS3可當成標記對應於第9、13、14及20條虛擬染色體之GG基因組染色體的標幟。比較這三個重複性序列在資料庫中的分佈與螢光原位雜交之結果,證實該資料庫可用於預測重複性序列在染色體上可能分佈的位置,但不適合用於評估基因組內重複性序列之重複數。 利用點轉漬雜合篩選出兩個有用的ISSR (inter simple sequence repeat) 擴增片段,其中SBRS4可用於GG基因組之染色體核型分析。而SBRS5可做為G. tomentella (DDD1D1) 基因組及其中兩對染色體之標幟。本實驗同時指出,物種間之非專一重複性片段因其在不同基因組內分佈狀況的差異,仍能用於標定特定基因組及染色體,解決部份大豆屬物種染色體研究困難的問題。 | zh_TW |
dc.description.abstract | Soybean (Glycine max (L.) Merr.) is the most important crop in the Leguminosae families. And the wild Glycine species are very momentous breeding resources for soybean improvement. Taiwan is the northern boundary of the annual wild soybeans, and the southern boundary of the perennial wild soybeans. They are the annual one G. soja (GG genome) , also the three perennial ones G. tomentella (DDD1D1 genome) , G. dolichocarpa (A6A6DD genome) and G. tabacina (A6A6B3B3 genome) .
The basic chromosome number of the Glycine species is 2n=40. Their chromosomes are similar in size and lacks of obvious morphological characters. Little is known about the repeat sequences composition in the genomes of the Glycine species, hence the research about genome taxonomy and chromosome karyotyping are limited. Using fluorescent in situ hybridization (FISH) method, target sequences can be labeled with repeat sequences, and thus labeling specific genomes or the particular regions of chromosomes. FISH can also be used to reveal the genome diversity between species, and perform karyotyping. Thus, it is helpful to solve the problems among taxonomy controversies or chromosome or studies. Three G. max repeat sequences were isolated by bioinformatics analysis using soybean whole-genome shotgun (WGS) sequences, and designated SBRS1、SBRS2 and SBRS3. SBRS1 could be used on the special specific probe of the GG genome. SBRS2 and SBRS3 could be used to label the pseudomolecules 9, 13, 14 and 20 in the GG genome. The searches were carried out, and then the compared with the result of FISH. Thus, it indicated that the WGS data could be used for predicting the distribution of repeats, while not suitable for estimating the amount of these repeat sequences. Two useful ISSR amplified fragments are selected by Dot blotting hybridization. The SBRS4 could be used for GG genomic chromosomes karyotyping. And the SBRS5 could be a specific probe of the G. tomentella genome and would label two pairs of chromosomes. It is thus suggested that, the unspecific sequences still may offer the potential to be applied as the genomic or chromosome markers. | en |
dc.description.provenance | Made available in DSpace on 2021-06-15T04:03:38Z (GMT). No. of bitstreams: 1 ntu-99-R96621122-1.pdf: 1411857 bytes, checksum: 301a0bb1bb0d64eeccbca0c975ae92dd (MD5) Previous issue date: 2010 | en |
dc.description.tableofcontents | 中文摘要 I
ABSTRACT II 壹、 前言 1 貳、 前人研究 4 (一) 重複性DNA序列之探討與應用 4 (二) 大豆基因組及其重複性序列之研究 12 (三) 分佈在台灣地區的大豆屬 (genus Glycine) 之物種 17 (四) 螢光原位雜交技術 19 參、 材料方法 21 (一) 植物材料 21 (二) 利用生物資訊計算大豆基因組內重複性序列之重複數 21 (三) 葉片DNA萃取 22 (四) 擴增基因組內重複性序列及ISSR 23 (五) TA cloning 23 (六) 選殖片段之定序前處理 24 (七) 點轉漬 (Dot Blotting) 25 (八) 南方氏轉漬 (Southern Blot) 26 (九) 雜合反應 (Hybridization) 26 (十) 染色體玻片製備 27 (十一) 探針合成 28 (十二) 螢光原位雜交 (fluorescent in situ hybridization, FISH) 29 肆、 結果 31 (一) SB92衛星序列 31 (二) 大豆WGS定序計畫資料庫內所得之重複性序列 32 (三) 點轉漬雜合篩選所得之高度重複ISSR擴增片段 36 (四) 45S rDNA重複性序列 38 伍、 討論 39 (五) 點轉漬雜合篩選所得之高度重複ISSR擴增片段 42 (六) 45S rDNA 43 陸、 結論 44 柒、 參考文獻 45 捌、 圖 59 (一) 圖 1、以螢光原位雜交標記SB92衛星序列在G. soja細胞染 色體 (質) 之結果 59 (二) 圖 2、以螢光原位雜交標記SBRS1在G. soja細胞染 色體 (質) 之結果 60 (三) 圖 3、以螢光原位雜交標記SBRS1在G. tomentella染 色體之結果 61 (四) 圖 4、以螢光原位雜交標記SBRS1在G. dolichocarpa及G. tabacina細胞染色體之結果 62 (五) 圖5、以SBRS1為探針之南方氏轉漬膜雜交的結果 63 (六) 圖 6、以螢光原位雜交標記SBRS2在G. soja細胞染色體之結果 64 (七) 圖 7、以螢光原位雜交標記SBRS2在G. tomentella、G. dolichocarpa及G. tabacina細胞染色體之結果 65 (八) 圖 8、以SBRS2為探針之南方氏轉漬膜雜交的結果 66 (九) 圖 9、以螢光原位雜交標記野生大豆體細胞染色體之結果 67 (十) 圖 10、以SBRS3為探針之南方氏轉漬膜雜交的結果 68 (十一) 圖 11、以G. max基因組DNA做為探針對93個ISSR擴增片段進行點轉漬雜合之結果 69 (十二) 圖 12、以G. tomentella基因組DNA做為探針對93個ISSR擴增片段進行點轉漬雜合之結果 70 (十三) 圖 13、以G. dolichocarpa基因組DNA做為探針對93個ISSR擴增片段進行點轉漬雜合之結果 71 (十四) 圖 14、 G. tabacina基因組DNA做為探針對93個ISSR擴增片段進行點轉漬雜合之結果 72 (十五) 圖 15、以螢光原位雜交 (FISH) 標記第SBRS4在G. soja根尖之體細胞分裂中期染色體之結果 73 (十六) 圖 16、以SBRS4為探針之南方氏轉漬膜雜交的結果 74 (十七) 圖 17、以螢光原位雜交 (FISH) 標記第SBRS5在G. tomentella根尖之體細胞分裂中期染色體之結果 75 (十八) 圖 18、以SBRS5為探針之南方氏轉漬膜雜交的結果 76 (十九) 圖 19、以螢光原位雜交 (FISH) 標記45S rDNA在G. tabacina根尖之細胞分裂中期染色體之結果 77 玖、 附表 78 (一) 表 1、引子序列 78 (二) 表 2、探針序列 79 (三) 表 3 台灣地區野生大豆搜集系資料 80 | |
dc.language.iso | zh-TW | |
dc.title | 台灣野生大豆基因組與染色體標幟之初探 | zh_TW |
dc.title | Preliminary studies on the genome and chromosome markers of wild soybeans collected in Taiwan | en |
dc.type | Thesis | |
dc.date.schoolyear | 98-1 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 邢禹依,鍾美珠,張松彬 | |
dc.subject.keyword | 大豆,大豆屬,染色體,螢光原位雜交,染色體標幟,基因組, | zh_TW |
dc.subject.keyword | sybean,Glycine max,Glycine,chromosome,FISH,fluorescent in situ hybridization,chromosome marker,genome, | en |
dc.relation.page | 80 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2010-02-11 | |
dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
dc.contributor.author-dept | 農藝學研究所 | zh_TW |
顯示於系所單位: | 農藝學系 |
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