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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 林大盛 | |
dc.contributor.author | Hung-Li Lin | en |
dc.contributor.author | 林紘立 | zh_TW |
dc.date.accessioned | 2021-06-15T04:02:35Z | - |
dc.date.available | 2015-03-11 | |
dc.date.copyright | 2010-03-11 | |
dc.date.issued | 2010 | |
dc.date.submitted | 2010-02-12 | |
dc.identifier.citation | Aderem, A. (2003) Phagocytosis and the inflammatory response. J Infect Dis 187 Suppl 2, S340-5.
Akiyama, H., Sato, Y., Watanabe, T., Nagaoka, M.H., Yoshioka, Y., Shoji, T., Kanda, T., Yamada, K., Totsuka, M., Teshima, R., Sawada, J., Goda, Y. and Maitani, T. (2005) Dietary unripe apple polyphenol inhibits the development of food allergies in murine models. FEBS Lett 579, 4485-91. Ali, S. and Mann, D.A. (2004) Signal transduction via the NF-kappaB pathway: a targeted treatment modality for infection, inflammation and repair. Cell Biochem Funct 22, 67-79. Almand, B., Clark, J.I., Nikitina, E., van Beynen, J., English, N.R., Knight, S.C., Carbone, D.P. and Gabrilovich, D.I. (2001) Increased production of immature myeloid cells in cancer patients: a mechanism of immunosuppression in cancer. J Immunol 166, 678-89. Auffray, C., Sieweke, M.H. and Geissmann, F. (2009) Blood monocytes: development, heterogeneity, and relationship with dendritic cells. Annu Rev Immunol 27, 669-92. Bronte, V. and Zanovello, P. (2005) Regulation of immune responses by L-arginine metabolism. Nat Rev Immunol 5, 641-54. Bunt, S.K., Sinha, P., Clements, V.K., Leips, J. and Ostrand-Rosenberg, S. (2006) Inflammation induces myeloid-derived suppressor cells that facilitate tumor progression. J Immunol 176, 284-90. Bunt, S.K., Yang, L., Sinha, P., Clements, V.K., Leips, J. and Ostrand-Rosenberg, S. (2007) Reduced inflammation in the tumor microenvironment delays the accumulation of myeloid-derived suppressor cells and limits tumor progression. Cancer Res 67, 10019-26. Cao, H., Urban, J.F., Jr. and Anderson, R.A. (2008) Cinnamon polyphenol extract affects immune responses by regulating anti- and proinflammatory and glucose transporter gene expression in mouse macrophages. J Nutr 138, 833-40. Chang, L.Y., Wan, H.C., Lai, Y.L., Kuo, Y.F., Liu, T.Y., Chen, Y.T. and Hung, S.L. (2009) Areca nut extracts increased expression of inflammatory cytokines, tumor necrosis factor-alpha, interleukin-1beta, interleukin-6 and interleukin-8, in peripheral blood mononuclear cells. J Periodontal Res 44, 175-83. Chang, L.Y., Wan, H.C., Lai, Y.L., Liu, T.Y. and Hung, S.L. (2006) Enhancing effects of areca nut extracts on the production of interleukin-6 and interleukin-8 by peripheral blood mononuclear cells. J Periodontol 77, 1969-77. Chang, M.C., Chiang, C.P., Lin, C.L., Lee, J.J., Hahn, L.J. and Jeng, J.H. (2005) Cell-mediated immunity and head and neck cancer: with special emphasis on betel quid chewing habit. Oral Oncol 41, 757-75. Chang, M.C., Wu, H.L., Lee, J.J., Lee, P.H., Chang, H.H., Hahn, L.J., Lin, B.R., Chen, Y.J. and Jeng, J.H. (2004) The induction of prostaglandin E2 production, interleukin-6 production, cell cycle arrest, and cytotoxicity in primary oral keratinocytes and KB cancer cells by areca nut ingredients is differentially regulated by MEK/ERK activation. J Biol Chem 279, 50676-83. Chen, J. and Liu, X. (2009) The role of interferon gamma in regulation of CD4+ T-cells and its clinical implications. Cell Immunol 254, 85-90. Chiang, C.P., Wu, H.Y., Liu, B.Y., Wang, J.T. and Kuo, M.Y. (2002) Quantitative analysis of immunocompetent cells in oral submucous fibrosis in Taiwan. Oral Oncol 38, 56-63. Chu, N.S. (2002) Neurological aspects of areca and betel chewing. Addict Biol 7, 111-4. Crooks, S.W. and Stockley, R.A. (1998) Leukotriene B4. Int J Biochem Cell Biol 30, 173-8. Dasgupta, R., Saha, I., Pal, S., Bhattacharyya, A., Sa, G., Nag, T.C., Das, T. and Maiti, B.R. (2006) Immunosuppression, hepatotoxicity and depression of antioxidant status by arecoline in albino mice. Toxicology 227, 94-104. Diaz-Montero, C.M., Salem, M.L., Nishimura, M.I., Garrett-Mayer, E., Cole, D.J. and Montero, A.J. (2009) Increased circulating myeloid-derived suppressor cells correlate with clinical cancer stage, metastatic tumor burden, and doxorubicin-cyclophosphamide chemotherapy. Cancer Immunol Immunother 58, 49-59. Gabrilovich, D.I. and Nagaraj, S. (2009) Myeloid-derived suppressor cells as regulators of the immune system. Nat Rev Immunol 9, 162-74. Geissmann, F., Auffray, C., Palframan, R., Wirrig, C., Ciocca, A., Campisi, L., Narni-Mancinelli, E. and Lauvau, G. (2008) Blood monocytes: distinct subsets, how they relate to dendritic cells, and their possible roles in the regulation of T-cell responses. Immunol Cell Biol 86, 398-408. Gilani, A.H., Ghayur, M.N., Saify, Z.S., Ahmed, S.P., Choudhary, M.I. and Khalid, A. (2004) Presence of cholinomimetic and acetylcholinesterase inhibitory constituents in betel nut. Life Sci 75, 2377-89. Greifenberg, V., Ribechini, E., Rossner, S. and Lutz, M.B. (2009) Myeloid-derived suppressor cell activation by combined LPS and IFN-gamma treatment impairs DC development. Eur J Immunol 39, 2865-76. Gupta, P.C. and Warnakulasuriya, S. (2002) Global epidemiology of areca nut usage. Addict Biol 7, 77-83. Haque, M.F., Meghji, S., Khitab, U. and Harris, M. (2000) Oral submucous fibrosis patients have altered levels of cytokine production. J Oral Pathol Med 29, 123-8. Hoyer, K.K., Dooms, H., Barron, L. and Abbas, A.K. (2008) Interleukin-2 in the development and control of inflammatory disease. Immunol Rev 226, 19-28. Huang, B., Pan, P.Y., Li, Q., Sato, A.I., Levy, D.E., Bromberg, J., Divino, C.M. and Chen, S.H. (2006) Gr-1+CD115+ immature myeloid suppressor cells mediate the development of tumor-induced T regulatory cells and T-cell anergy in tumor-bearing host. Cancer Res 66, 1123-31. Huang, P.L., Chi, C.W. and Liu, T.Y. (2009) Effects of Areca catechu L. containing procyanidins on cyclooxygenase-2 expression in vitro and in vivo. Food Chem Toxicol. Hung, S.L., Cheng, Y.Y., Peng, J.L., Chang, L.Y., Liu, T.Y. and Chen, Y.T. (2005) Inhibitory effects of areca nut extracts on phagocytosis of Actinobacillus actinomycetemcomitans ATCC 33384 by neutrophils. J Periodontol 76, 373-9. Hung, S.L., Lee, Y.Y., Liu, T.Y., Peng, J.L., Cheng, Y.Y. and Chen, Y.T. (2006) Modulation of phagocytosis, chemotaxis, and adhesion of neutrophils by areca nut extracts. J Periodontol 77, 579-85. Hung, S.L., Lin, Y.J., Chien, E.J., Liu, W.G., Chang, H.W., Liu, T.Y. and Chen, Y.T. (2007) Areca nut extracts-activated secretion of leukotriene B4, and phosphorylation of p38 mitogen-activated protein kinase and elevated intracellular calcium concentrations in human polymorphonuclear leukocytes. J Periodontal Res 42, 393-401. IARC. (2004) Betel-quid and areca-nut chewing and some areca-nut derived nitrosamines. IARC Monogr Eval Carcinog Risks Hum 85, 1-334. Jeng, J.H., Chang, M.C. and Hahn, L.J. (2001) Role of areca nut in betel quid-associated chemical carcinogenesis: current awareness and future perspectives. Oral Oncol 37, 477-92. Jeng, J.H., Ho, Y.S., Chan, C.P., Wang, Y.J., Hahn, L.J., Lei, D., Hsu, C.C. and Chang, M.C. (2000) Areca nut extract up-regulates prostaglandin production, cyclooxygenase-2 mRNA and protein expression of human oral keratinocytes. Carcinogenesis 21, 1365-70. Jeng, J.H., Wang, Y.J., Chiang, B.L., Lee, P.H., Chan, C.P., Ho, Y.S., Wang, T.M., Lee, J.J., Hahn, L.J. and Chang, M.C. (2003) Roles of keratinocyte inflammation in oral cancer: regulating the prostaglandin E2, interleukin-6 and TNF-alpha production of oral epithelial cells by areca nut extract and arecoline. Carcinogenesis 24, 1301-15. Jewett, A., Head, C. and Cacalano, N.A. (2006) Emerging mechanisms of immunosuppression in oral cancers. J Dent Res 85, 1061-73. Jia, T., Serbina, N.V., Brandl, K., Zhong, M.X., Leiner, I.M., Charo, I.F. and Pamer, E.G. (2008) Additive roles for MCP-1 and MCP-3 in CCR2-mediated recruitment of inflammatory monocytes during Listeria monocytogenes infection. J Immunol 180, 6846-53. Kenny, T.P., Keen, C.L., Schmitz, H.H. and Gershwin, M.E. (2007) Immune effects of cocoa procyanidin oligomers on peripheral blood mononuclear cells. Exp Biol Med (Maywood) 232, 293-300. Kidd, P. (2003) Th1/Th2 balance: the hypothesis, its limitations, and implications for health and disease. Altern Med Rev 8, 223-46. Kim, H.P., Son, K.H., Chang, H.W. and Kang, S.S. (2004) Anti-inflammatory plant flavonoids and cellular action mechanisms. J Pharmacol Sci 96, 229-45. Ko, Y.C., Chiang, T.A., Chang, S.J. and Hsieh, S.F. (1992) Prevalence of betel quid chewing habit in Taiwan and related sociodemographic factors. J Oral Pathol Med 21, 261-4. Kolodziej, H. and Kiderlen, A.F. (2005) Antileishmanial activity and immune modulatory effects of tannins and related compounds on Leishmania parasitised RAW 264.7 cells. Phytochemistry 66, 2056-71. Laad, A., Kode, J., Chavan, S., Rao, R., Fakih, A.R. and Chiplunkar, S. (1996) Limiting dilution analysis of proliferating and cytotoxic lymphocytes in the peripheral blood and tumours of oral cancer patients. Eur J Cancer B Oral Oncol 32B, 337-42. Lai, Y.L., Lin, J.C., Yang, S.F., Liu, T.Y. and Hung, S.L. (2007) Areca nut extracts reduce the intracellular reactive oxygen species and release of myeloperoxidase by human polymorphonuclear leukocytes. J Periodontal Res 42, 69-76. Li, H., Han, Y., Guo, Q., Zhang, M. and Cao, X. (2009) Cancer-expanded myeloid-derived suppressor cells induce anergy of NK cells through membrane-bound TGF-beta 1. J Immunol 182, 240-9. Lin, W.Y., Chiu, T.Y., Lee, L.T., Lin, C.C., Huang, C.Y. and Huang, K.C. (2008) Betel nut chewing is associated with increased risk of cardiovascular disease and all-cause mortality in Taiwanese men. Am J Clin Nutr 87, 1204-11. Liu, T.Y., Chen, C.L. and Chi, C.W. (1996) Oxidative damage to DNA induced by areca nut extract. Mutat Res 367, 25-31. Mackenzie, G.G., Carrasquedo, F., Delfino, J.M., Keen, C.L., Fraga, C.G. and Oteiza, P.I. (2004) Epicatechin, catechin, and dimeric procyanidins inhibit PMA-induced NF-kappaB activation at multiple steps in Jurkat T cells. FASEB J 18, 167-9. Mackey, M.F., Barth, R.J., Jr. and Noelle, R.J. (1998) The role of CD40/CD154 interactions in the priming, differentiation, and effector function of helper and cytotoxic T cells. J Leukoc Biol 63, 418-28. Manach, C., Williamson, G., Morand, C., Scalbert, A. and Remesy, C. (2005) Bioavailability and bioefficacy of polyphenols in humans. I. Review of 97 bioavailability studies. Am J Clin Nutr 81, 230S-242S. Mao, T.K., van de Water, J., Keen, C.L., Schmitz, H.H. and Gershwin, M.E. (2002) Modulation of TNF-alpha secretion in peripheral blood mononuclear cells by cocoa flavanols and procyanidins. Dev Immunol 9, 135-41. McHeyzer-Williams, M., McHeyzer-Williams, L., Panus, J., Pogue-Caley, R., Bikah, G., Driver, D. and Eisenbraun, M. (2003) Helper T-cell-regulated B-cell immunity. Microbes Infect 5, 205-12. Miyake, M., Ide, K., Sasaki, K., Matsukura, Y., Shijima, K. and Fujiwara, D. (2008) Oral administration of highly oligomeric procyanidins of Jatoba reduces the severity of collagen-induced arthritis. Biosci Biotechnol Biochem 72, 1781-8. Miyake, M., Sasaki, K., Ide, K., Matsukura, Y., Shijima, K. and Fujiwara, D. (2006) Highly oligomeric procyanidins ameliorate experimental autoimmune encephalomyelitis via suppression of Th1 immunity. J Immunol 176, 5797-804. Nair, U., Bartsch, H. and Nair, J. (2004) Alert for an epidemic of oral cancer due to use of the betel quid substitutes gutkha and pan masala: a review of agents and causative mechanisms. Mutagenesis 19, 251-62. Ngoc, P.L., Gold, D.R., Tzianabos, A.O., Weiss, S.T. and Celedon, J.C. (2005) Cytokines, allergy, and asthma. Curr Opin Allergy Clin Immunol 5, 161-6. Ostrand-Rosenberg, S. and Sinha, P. (2009) Myeloid-derived suppressor cells: linking inflammation and cancer. J Immunol 182, 4499-506. Pak, A.S., Wright, M.A., Matthews, J.P., Collins, S.L., Petruzzelli, G.J. and Young, M.R. (1995) Mechanisms of immune suppression in patients with head and neck cancer: presence of CD34(+) cells which suppress immune functions within cancers that secrete granulocyte-macrophage colony-stimulating factor. Clin Cancer Res 1, 95-103. Ramiro-Puig, E., Perez-Cano, F.J., Ramirez-Santana, C., Castellote, C., Izquierdo-Pulido, M., Permanyer, J., Franch, A. and Castell, M. (2007) Spleen lymphocyte function modulated by a cocoa-enriched diet. Clin Exp Immunol 149, 535-42. Rodriguez, P.C. and Ochoa, A.C. (2008) Arginine regulation by myeloid derived suppressor cells and tolerance in cancer: mechanisms and therapeutic perspectives. Immunol Rev 222, 180-91. Russell, D.G., Vanderven, B.C., Glennie, S., Mwandumba, H. and Heyderman, R.S. (2009) The macrophage marches on its phagosome: dynamic assays of phagosome function. Nat Rev Immunol 9, 594-600. Seder, R.A. and Paul, W.E. (1994) Acquisition of lymphokine-producing phenotype by CD4+ T cells. Annu Rev Immunol 12, 635-73. Serafini, P., De Santo, C., Marigo, I., Cingarlini, S., Dolcetti, L., Gallina, G., Zanovello, P. and Bronte, V. (2004) Derangement of immune responses by myeloid suppressor cells. Cancer Immunol Immunother 53, 64-72. Sinha, P., Clements, V.K., Bunt, S.K., Albelda, S.M. and Ostrand-Rosenberg, S. (2007a) Cross-talk between myeloid-derived suppressor cells and macrophages subverts tumor immunity toward a type 2 response. J Immunol 179, 977-83. Sinha, P., Clements, V.K., Fulton, A.M. and Ostrand-Rosenberg, S. (2007b) Prostaglandin E2 promotes tumor progression by inducing myeloid-derived suppressor cells. Cancer Res 67, 4507-13. Solovjov, D.A., Pluskota, E. and Plow, E.F. (2005) Distinct roles for the alpha and beta subunits in the functions of integrin alphaMbeta2. J Biol Chem 280, 1336-45. Sullivan, R.J. and Hagen, E.H. (2002) Psychotropic substance-seeking: evolutionary pathology or adaptation? Addiction 97, 389-400. Suzuki, E., Kapoor, V., Jassar, A.S., Kaiser, L.R. and Albelda, S.M. (2005) Gemcitabine selectively eliminates splenic Gr-1+/CD11b+ myeloid suppressor cells in tumor-bearing animals and enhances antitumor immune activity. Clin Cancer Res 11, 6713-21. Terra, X., Valls, J., Vitrac, X., Merrillon, J.M., Arola, L., Ardevol, A., Blade, C., Fernandez-Larrea, J., Pujadas, G., Salvado, J. and Blay, M. (2007) Grape-seed procyanidins act as antiinflammatory agents in endotoxin-stimulated RAW 264.7 macrophages by inhibiting NFkB signaling pathway. J Agric Food Chem 55, 4357-65. Trinchieri, G. (1997) Cytokines acting on or secreted by macrophages during intracellular infection (IL-10, IL-12, IFN-gamma). Curr Opin Immunol 9, 17-23. Van Gool, S.W., Vandenberghe, P., de Boer, M. and Ceuppens, J.L. (1996) CD80, CD86 and CD40 provide accessory signals in a multiple-step T-cell activation model. Immunol Rev 153, 47-83. Verastegui, E., Morales, R., Barrera, J.L., Mueller, A., Guzman, B., Meneses, A. and Alfaro, G. (2002) Immunological approach in the evaluation of regional lymph nodes of patients with squamous cell carcinoma of the head and neck. Clin Immunol 102, 37-47. Wang, C.C., Huang, P.L., Liu, T.Y. and Jan, T.R. (2009a) Highly oligomeric procyanidins from areca nut induce lymphocyte apoptosis via the depletion of intracellular thiols. Toxicol In Vitro 23, 1234-41. Wang, C.C., Liu, T.Y., Cheng, C.H. and Jan, T.R. (2009b) Involvement of the mitochondrion-dependent pathway and oxidative stress in the apoptosis of murine splenocytes induced by areca nut extract. Toxicol In Vitro 23, 840-7. Wang, C.C., Liu, T.Y., Wey, S.P., Wang, F.I. and Jan, T.R. (2007) Areca nut extract suppresses T-cell activation and interferon-gamma production via the induction of oxidative stress. Food Chem Toxicol 45, 1410-8. Warnakulasuriya, S., Johnson, N.W. and van der Waal, I. (2007) Nomenclature and classification of potentially malignant disorders of the oral mucosa. J Oral Pathol Med 36, 575-80. Wu, Q., Yang, Y. and Simon, J.E. (2007) Qualitative and quantitative HPLC/MS determination of proanthocyanidins in areca nut (Areca catechu). Chem Biodivers 4, 2817-26. Yamakoshi, J., Kataoka, S., Koga, T. and Ariga, T. (1999) Proanthocyanidin-rich extract from grape seeds attenuates the development of aortic atherosclerosis in cholesterol-fed rabbits. Atherosclerosis 142, 139-49. Yang, C.S., Sang, S., Lambert, J.D. and Lee, M.J. (2008) Bioavailability issues in studying the health effects of plant polyphenolic compounds. Mol Nutr Food Res 52 Suppl 1, S139-51. Youn, J.I., Nagaraj, S., Collazo, M. and Gabrilovich, D.I. (2008) Subsets of myeloid-derived suppressor cells in tumor-bearing mice. J Immunol 181, 5791-802. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/45049 | - |
dc.description.abstract | 嚼食檳榔為導致口腔癌及口腔癌前病變最主要的病因之一。臨床研究指出長時間的口腔組織發炎以及病人免疫功能的惡化和嚼食檳榔所造成相關疾病有顯著的關聯性。近年來許多離體實驗指出檳榔子萃取物(areca nut extract; ANE)會影響淋巴球及噬中性球的功能,但ANE在活體中對免疫功能的作用仍有待釐清。本實驗利用卵白蛋白(ovalbumin; OVA)免疫小鼠的模式探討ANE對抗原專一性免疫反應的影響。於第1-5天及第8-12天每天1次以腹腔注射ANE (5-50 mg/kg)或經胃管投與ANE (50-200 mg/kg),小鼠以鋁膠(alum)吸附之OVA於第3天給予免疫,第13天於後肢足墊皮下注射OVA (10 microgram 溶於20 micorliter 生理食鹽水)以誘發遲發型過敏反應。實驗結果顯示腹腔注射ANE以劑量相關性的方式增加脾臟指數及脾臟中CD11b+Gr-1+骨髓衍生抑制細胞(myeloid-derived suppressor cells; MDSC)的比例。CD11b+細胞由顆粒球性及單核球性兩種型態的細胞所組成,其經由lipopolysaccharide (LPS)刺激後IL-10的產量在ANE處理的組別中顯著增加。此細胞的吞噬作用和表現TNF-alpha的細胞族群在ANE的作用下亦顯著上升。ANE也促進脾臟細胞分泌OVA專一性IFN-gamma以及LPS刺激之iNOS mRNA的表現。腹腔注射ANE (5和25 mg/kg)顯著抑制小鼠血清中OVA專一性IgM及IgG1的生成,而IgG2a的產量則在高劑量(50 mg/kg)有顯著上升。局部經由OVA刺激所引起的足墊腫脹、CD3+ 和F4/80+炎症細胞的浸潤,以及IFN-gamma陽性細胞的數量,在腹腔注射ANE組皆有明顯的增加。綜合上述,腹腔注射ANE具有促進抗原專一性IFN-gamma表現、引起發炎反應,以及促進CD11b+Gr-1+骨髓衍生抑制細胞生成的作用。此發炎反應可能促使具有分泌IL-10能力的骨髓衍生抑制細胞族群增加。這些細胞抑制免疫功能的特性仍有待後續實驗進一步釐清。 | zh_TW |
dc.description.abstract | Areca nut chewing is an etiological factor for oral cancer and pre-cancer lesions. Clinical studies suggest that prolonged tissue inflammation and immune deterioration are closely associated with the pathophysiology of areca-related oral diseases. Although recent in vitro studies demonstrated that areca nut extract (ANE) influenced the functionality of lymphocytes and neutrophils, the in vivo immunomodulatory effect of areca nut ingredients remains mostly unclear. The present studies investigated the effect of ANE on antigen-specific immune responses in ovalbumin (OVA)-sensitized mice. BALB/c mice were daily administered with ANE by intraperitoneal (IP; 5-50 mg/kg) injection or oral gavage (50-200 mg/kg) from day 1-5 and 8-12. The mice were IP sensitized with ovalbumin (OVA) absorbed to alum on day 3 and their footpads were subcutaneously challenged with OVA (10 microgram in 20 microliter saline) on day 13 to induce delayed-type hypersensitivity (DTH) reactions. IP administration of ANE dose-dependently increased the spleen index and the number of CD11b+Gr-1+ myeloid-derived suppressor cells (MDSC) in the spleen. Morphologic examination revealed that the CD11b+ cells included granulocytic and monocytic cells. Lipopolysaccharide (LPS)-induced IL-10 production by CD11b+ cells of IP ANE-treated mice was significantly increased. The phagocytic activity and TNF-alpha expression by the splenic CD11b+ cells were elevated in ANE-treated groups. IP ANE also enhanced OVA-specific IFN-gamma production and LPS-induced iNOS mRNA expression by splenocytes. The serum levels of OVA-specific IgM and IgG1 was significantly attenuated in mice treated with IP ANE (5 and 25 mg/kg), whereas OVA-specific IgG2a were enhanced in mice treated with the high dose of ANE (50 mg/kg). In addition, an increase in OVA challenge-induced footpad swelling, and a greater infiltration of inflammatory cells, including CD3+ and F4/80+ cells was observed in ANE-treated mice. The number of IFN-gamma secreting cells in the footpads was also increased. Taken together, these results demonstrated that IP administration of ANE enhanced the production of antigen-specific IFN-gamma, produced a pro-inflammatory effect, and induced the development of CD11b+Gr-1+ MDSC. The inflammation may induce the accumulation of CD11b+Gr-1+ MDSC which have the ability to secret IL-10. Further studies were needed to clarify the suppressive nature of the MDSC. | en |
dc.description.provenance | Made available in DSpace on 2021-06-15T04:02:35Z (GMT). No. of bitstreams: 1 ntu-99-R96629027-1.pdf: 1827968 bytes, checksum: 849e8d1e7a5d411aa0178ea79c5e82ac (MD5) Previous issue date: 2010 | en |
dc.description.tableofcontents | 致謝 I
中文摘要 III Abstract IV Contents VII Figures XI Tables XIII Abbreviations XIV Chapter 1 Introduction 1 1.1 Areca quid chewing and background 1 1.2 Areca quid and the constituents of areca nut 1 1.3 Immune deterioration in patients who have AQ chewing habit 3 1.4 The influence of areca nut extract (ANE) on immunocompetent cells 5 1.5 The immunomodulatory effect of polyphonols and proanthocyanidins (PAC) 6 1.6 The function of macrophages and monocytes 8 1.7 The differentiation of Th cells 10 1.8 Delayed-type hypersensitivity 12 1.9 Myeloid-derived suppressor cells (MDSC) 12 1.10 Objective of the study 15 Chapter 2 Materials and Methods 17 2.1 Reagents and areca nut extract 17 2.2 Animals 18 2.3 Protocol of ANE administration, and OVA sensitization and challenge 18 2.4 Measurement of spleen enlargement 21 2.5 Culture of splenocytes 21 2.6 Measurement of metabolic activity by MTT assay 21 2.7 Measurement of OVA-specific cytokines by ELISA 22 2.8 Measurement of serum OVA-specific IgM, IgG2a, IgG1 by ELISA 23 2.9 Analysis of the cellularity and lymphocyte subsets of splenocytes 24 2.10 RNA isolation 24 2.11 Reverse transcriptase-polymerase chain reaction (RT-PCR) 25 2.12 Isolation of CD11b+ cells 26 2.13 Analysis of the morphology of CD11b+ cells 27 2.14 Analysis of the phagocytosis of CD11b+ cells 27 2.15 Analysis of CD11b+ intracellular TNF-alpha 27 2.16 Analysis of the surface marker expression of CD11b+ cells 27 2.17 Measurement of LPS-induced IL-10 by ELISA 28 2.18 Histological examination of footpads 28 2.19 Immunohistochemical staining 28 2.20 Quantification of the inflammatory cells by IHC staining 29 2.21 Statistical analysis 29 Chapter 3 Results 31 3.1 Effect of ANE administration on the body weight of mice 31 3.2 Effects of ANE on the spleen index and cellularity of splenocytes 31 3.3 Effect of ANE on the metabolic activity of splenocytes 32 3.4 Effect of ANE on OVA-specific IFN-gamma and IL-2 production by spenocytes 32 3.5 Effect of IP administration of ANE on OVA-specific serum IgM, IgG2a and IgG1 33 3.6 Effect of IP administration of ANE on iNOS expression in splenocytes 33 3.7 CD11b+ cells in ANE-treated mice are heterogeneous populations 34 3.8 Effect of IP administration of ANE on phagocytic activity of CD11b+ cells 34 3.9 Effect of IP administration of ANE on the production of TNF-alpha by CD11b+ cells 35 3.10 Effect of IP administration of ANE on the expression of CD40, CD80, CD86 and MHC class II by CD11b+ cells 35 3.11 IP administration of ANE enhanced the cellularity of CD11b+Gr-1+ cells in the spleen 36 3.12 IP administration of ANE enhanced LPS-induced IL-10 production by CD11b+ cells and splenocytes 36 3.13 Effect of IP administration of ANE on DTH reactions 37 Chapter 4 Discussion 61 References 69 | |
dc.language.iso | en | |
dc.title | 檳榔子萃取物誘發卵白蛋白免疫小鼠發炎反應及促進骨髓衍生抑制細胞之生成 | zh_TW |
dc.title | Areca Nut Extract Induced Inflammatory Reactions and Enhanced the Development of Myeloid-derived Suppressor Cells in Ovalbumin-sensitized Mice | en |
dc.type | Thesis | |
dc.date.schoolyear | 98-1 | |
dc.description.degree | 碩士 | |
dc.contributor.coadvisor | 詹東榮 | |
dc.contributor.oralexamcommittee | 張芳嘉,陳惠諴,詹銘煥 | |
dc.subject.keyword | 檳榔子萃取物,骨髓衍生抑制細胞,珈瑪干擾素,發炎反應, | zh_TW |
dc.subject.keyword | areca nut extract,myeloid-derived suppressor cell,IFN-gamma,inflammation, | en |
dc.relation.page | 75 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2010-02-12 | |
dc.contributor.author-college | 獸醫專業學院 | zh_TW |
dc.contributor.author-dept | 獸醫學研究所 | zh_TW |
顯示於系所單位: | 獸醫學系 |
文件中的檔案:
檔案 | 大小 | 格式 | |
---|---|---|---|
ntu-99-1.pdf 目前未授權公開取用 | 1.79 MB | Adobe PDF |
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。