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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 胡凱康 | |
dc.contributor.author | Chia-Lin Chou | en |
dc.contributor.author | 周佳霖 | zh_TW |
dc.date.accessioned | 2021-05-14T17:42:44Z | - |
dc.date.available | 2015-08-26 | |
dc.date.available | 2021-05-14T17:42:44Z | - |
dc.date.copyright | 2015-08-26 | |
dc.date.issued | 2015 | |
dc.date.submitted | 2015-08-14 | |
dc.identifier.citation | 丁文彥、林家玉、黃秋蘭、江瑞拱(2013)水稻新品種臺東33號之育成。臺東區農業改良場研究彙報 23: 1–16。
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dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/4500 | - |
dc.description.abstract | 本研究從水稻44K SNP晶片分析得到35,746筆包含413個廣泛來源的水稻基因型分析結果,經由篩選成功讀值率、SNP類型、對偶基因位置、次要對偶基因型頻度等資訊,得到表現較佳的130個SNP基因座,再經實驗測試,先選擇可判讀且於次族群中遺傳距離較遠的品種間具多型性的47個基因座後,對本研究收集到的49個目標品種基因型分析,挑選出適用於我國優良水稻推薦品種組合的10個SNP組成水稻SNP品種鑑別套件。使用的10個SNP標誌在49個包含我國優良水稻推薦品種的水稻品種間之平均PIC值為0.402,於49個品種間共1,176個兩兩組合之中,僅無法鑑別‘臺南16號’/‘越光’、‘臺東30號’/‘臺東33號’、‘臺中秈糯1號’/‘臺中秈糯2號’等背景相似度極高的三組品種。從篩選品種中選取遺傳距離最大且不具品種權保護的3個品種組成標準品種,其涵蓋100%的對偶基因型。 | zh_TW |
dc.description.abstract | The goal of this study is to develop a set of single nucleotide polymorphism (SNP) markers for identification of Taiwan rice varieties. At first, 130 candidate SNP were selected based on call rates, SNP types, allele positions and minor allele frequency (MAF) from 35,746 SNP datasets which derived from 413 rice varieties analyzed by rice 44K SNP microarray chip. By laboratory analysis around 130 SNP and 8 genetically distinct japonica Taiwan rice varieties, we narrowed down the candidate SNP into 47 SNP loci. After genotyping 49 rice varieties with these 47 SNP markers, we found that a selected set of 10 SNP markers was capable to distinguish 1,173 pairs out of total 1,176 variety pairs except 3 pairs of closly related varieties, ‘TN16’/‘Koshihikari’, ‘TT30’/‘TT33’ and ‘TCSG1’/‘TCSG2’. The average of polymorphism information contents (PICs) is 0.402 for the selected set. We also selected 3 varieties as reference varieties that they have largest genetic distance within 49 Taiwan rice varieties and all without protect by Plant Variety Right. The reference varieties cover 100% of the alleles of 49 rice varieties used in this study. | en |
dc.description.provenance | Made available in DSpace on 2021-05-14T17:42:44Z (GMT). No. of bitstreams: 1 ntu-104-R99621119-1.pdf: 1942971 bytes, checksum: d68c74dc77e9065de8928a32559db227 (MD5) Previous issue date: 2015 | en |
dc.description.tableofcontents | 誌謝 i
中文摘要 ii ABSTRACT iii 目錄 iv 圖目錄 vi 表目錄 vii 重要名詞縮寫對照表 viii 第一章 前言 9 第二章 前人研究 12 一、SNP探勘與分析 12 二、SNP用於品種鑑別 15 三、水稻SNP資料庫 15 四、水稻的核心收集 16 五、CoreHunter 18 第三章 材料與方法 20 一、試驗材料 20 二、水稻DNA萃取、定量與品質檢定 21 三、聚合酶連鎖反應與電泳分析 21 四、SNP基因座挑選 22 五、SNP分子標誌設計 23 六、評估SNP分子標誌與架構水稻SNP品種鑑別套件 23 七、驗證套件標誌組合 24 八、建立標準品種 24 第四章 結果與討論 29 一、SNP基因座挑選結果 29 二、驗證基因座挑選組合 30 三、架構臺灣水稻優良品種SNP鑑別套件 30 四、標準品種 31 五、開發階段對偶基因專一性引子之選擇 31 六、可能的分析系統 33 七、無法區別的臺灣品種 34 第五章 結論 45 參考文獻 48 | |
dc.language.iso | zh-TW | |
dc.title | 開發水稻之單一核苷酸多型性品種鑑別系統 | zh_TW |
dc.title | Development of Rice Variety Identification System using Single Nucleotide Polymorphic Markers | en |
dc.type | Thesis | |
dc.date.schoolyear | 103-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 林順福,張孟基,楊佐琦 | |
dc.subject.keyword | 水稻,品種鑑別,單一核?酸多型性, | zh_TW |
dc.subject.keyword | rice,variety identification,single nucleotide polymorphism (SNP), | en |
dc.relation.page | 59 | |
dc.rights.note | 同意授權(全球公開) | |
dc.date.accepted | 2015-08-14 | |
dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
dc.contributor.author-dept | 農藝學研究所 | zh_TW |
顯示於系所單位: | 農藝學系 |
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