請用此 Handle URI 來引用此文件:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/44954
完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 吳漢忠(Han-Chung Wu) | |
dc.contributor.author | Tung-Ying Lu | en |
dc.contributor.author | 呂東盈 | zh_TW |
dc.date.accessioned | 2021-06-15T03:59:22Z | - |
dc.date.available | 2015-09-09 | |
dc.date.copyright | 2010-09-09 | |
dc.date.issued | 2010 | |
dc.date.submitted | 2010-04-08 | |
dc.identifier.citation | Adhikary, S., and Eilers, M. (2005). Transcriptional regulation and transformation by Myc proteins. Nat Rev Mol Cell Biol 6, 635-645.
Agger, K., Cloos, P.A., Christensen, J., Pasini, D., Rose, S., Rappsilber, J., Issaeva, I., Canaani, E., Salcini, A.E., and Helin, K. (2007). UTX and JMJD3 are histone H3K27 demethylases involved in HOX gene regulation and development. Nature 449, 731-734. Al-Hajj, M., Wicha, M.S., Benito-Hernandez, A., Morrison, S.J., and Clarke, M.F. (2003). Prospective identification of tumorigenic breast cancer cells. Proc Natl Acad Sci U S A 100, 3983-3988. Anderson, R., Schaible, K., Heasman, J., and Wylie, C. (1999). Expression of the homophilic adhesion molecule, Ep-CAM, in the mammalian germ line. J Reprod Fertil 116, 379-384. Assou, S., Le Carrour, T., Tondeur, S., Strom, S., Gabelle, A., Marty, S., Nadal, L., Pantesco, V., Reme, T., Hugnot, J.P., et al. (2007). A meta-analysis of human embryonic stem cells transcriptome integrated into a web-based expression atlas. Stem Cells 25, 961-973. Avilion, A.A., Nicolis, S.K., Pevny, L.H., Perez, L., Vivian, N., and Lovell-Badge, R. (2003). Multipotent cell lineages in early mouse development depend on SOX2 function. Genes Dev 17, 126-140. Azuara, V., Perry, P., Sauer, S., Spivakov, M., Jorgensen, H.F., John, R.M., Gouti, M., Casanova, M., Warnes, G., Merkenschlager, M., et al. (2006). Chromatin signatures of pluripotent cell lines. Nat Cell Biol 8, 532-538. Balzar, M., Winter, M.J., de Boer, C.J., and Litvinov, S.V. (1999). The biology of the 17-1A antigen (Ep-CAM). J Mol Med 77, 699-712. Barski, A., Cuddapah, S., Cui, K., Roh, T.Y., Schones, D.E., Wang, Z., Wei, G., Chepelev, I., and Zhao, K. (2007). High-resolution profiling of histone methylations in the human genome. Cell 129, 823-837. Basak, S., Speicher, D., Eck, S., Wunner, W., Maul, G., Simmons, M.S., and Herlyn, D. (1998). Colorectal carcinoma invasion inhibition by CO17-1A/GA733 antigen and its murine homologue. J Natl Cancer Inst 90, 691-697. Behrens, J., Mareel, M.M., Van Roy, F.M., and Birchmeier, W. (1989). Dissecting tumor cell invasion: epithelial cells acquire invasive properties after the loss of uvomorulin-mediated cell-cell adhesion. J Cell Biol 108, 2435-2447. Bernstein, B.E., Kamal, M., Lindblad-Toh, K., Bekiranov, S., Bailey, D.K., Huebert, D.J., McMahon, S., Karlsson, E.K., Kulbokas, E.J., 3rd, Gingeras, T.R., et al. (2005). Genomic maps and comparative analysis of histone modifications in human and mouse. Cell 120, 169-181. Bernstein, B.E., Mikkelsen, T.S., Xie, X., Kamal, M., Huebert, D.J., Cuff, J., Fry, B., Meissner, A., Wernig, M., Plath, K., et al. (2006). A bivalent chromatin structure marks key developmental genes in embryonic stem cells. Cell 125, 315-326. Boyer, L.A., Lee, T.I., Cole, M.F., Johnstone, S.E., Levine, S.S., Zucker, J.P., Guenther, M.G., Kumar, R.M., Murray, H.L., Jenner, R.G., et al. (2005). Core transcriptional regulatory circuitry in human embryonic stem cells. Cell 122, 947-956. Boyer, L.A., Mathur, D., and Jaenisch, R. (2006a). Molecular control of pluripotency. Curr Opin Genet Dev 16, 455-462. Boyer, L.A., Plath, K., Zeitlinger, J., Brambrink, T., Medeiros, L.A., Lee, T.I., Levine, S.S., Wernig, M., Tajonar, A., Ray, M.K., et al. (2006b). Polycomb complexes repress developmental regulators in murine embryonic stem cells. Nature 441, 349-353. Brimble, S.N., Sherrer, E.S., Uhl, E.W., Wang, E., Kelly, S., Merrill, A.H., Jr., Robins, A.J., and Schulz, T.C. (2007). The cell surface glycosphingolipids SSEA-3 and SSEA-4 are not essential for human ESC pluripotency. Stem Cells 25, 54-62. Brown, D.T. (2001). Histone variants: are they functionally heterogeneous? Genome Biol 2, REVIEWS0006. Cao, R., and Zhang, Y. (2004). SUZ12 is required for both the histone methyltransferase activity and the silencing function of the EED-EZH2 complex. Mol Cell 15, 57-67. Cartwright, P., McLean, C., Sheppard, A., Rivett, D., Jones, K., and Dalton, S. (2005). LIF/STAT3 controls ES cell self-renewal and pluripotency by a Myc-dependent mechanism. Development 132, 885-896. Cedar, H., and Bergman, Y. (2009). Linking DNA methylation and histone modification: patterns and paradigms. Nat Rev Genet 10, 295-304. Chadwick, B.P., and Willard, H.F. (2001). A novel chromatin protein, distantly related to histone H2A, is largely excluded from the inactive X chromosome. J Cell Biol 152, 375-384. Chambers, I., Colby, D., Robertson, M., Nichols, J., Lee, S., Tweedie, S., and Smith, A. (2003). Functional expression cloning of Nanog, a pluripotency sustaining factor in embryonic stem cells. Cell 113, 643-655. Chambers, I., and Smith, A. (2004). Self-renewal of teratocarcinoma and embryonic stem cells. Oncogene 23, 7150-7160. Chan, K.K., Zhang, J., Chia, N.Y., Chan, Y.S., Sim, H.S., Tan, K.S., Oh, S.K., Ng, H.H., and Choo, A.B. (2009). KLF4 and PBX1 directly regulate NANOG expression in human embryonic stem cells. Stem Cells 27, 2114-2125. Chen, Y.C., Huang, H.N., Lin, C.T., Chen, Y.F., King, C.C., and Wu, H.C. (2007). Generation and characterization of monoclonal antibodies against dengue virus type 1 for epitope mapping and serological detection by epitope-based peptide antigens. Clin Vaccine Immunol 14, 404-411. Chew, J.L., Loh, Y.H., Zhang, W., Chen, X., Tam, W.L., Yeap, L.S., Li, P., Ang, Y.S., Lim, B., Robson, P., et al. (2005). Reciprocal transcriptional regulation of Pou5f1 and Sox2 via the Oct4/Sox2 complex in embryonic stem cells. Mol Cell Biol 25, 6031-6046. Choo, K.H. (2000). Centromerization. Trends Cell Biol 10, 182-188. Dalerba, P., Dylla, S.J., Park, I.K., Liu, R., Wang, X., Cho, R.W., Hoey, T., Gurney, A., Huang, E.H., Simeone, D.M., et al. (2007). Phenotypic characterization of human colorectal cancer stem cells. Proc Natl Acad Sci U S A 104, 10158-10163. Dan, Y.Y., Riehle, K.J., Lazaro, C., Teoh, N., Haque, J., Campbell, J.S., and Fausto, N. (2006). Isolation of multipotent progenitor cells from human fetal liver capable of differentiating into liver and mesenchymal lineages. Proc Natl Acad Sci U S A 103, 9912-9917. De Santa, F., Totaro, M.G., Prosperini, E., Notarbartolo, S., Testa, G., and Natoli, G. (2007). The histone H3 lysine-27 demethylase Jmjd3 links inflammation to inhibition of polycomb-mediated gene silencing. Cell 130, 1083-1094. Epsztejn-Litman, S., Feldman, N., Abu-Remaileh, M., Shufaro, Y., Gerson, A., Ueda, J., Deplus, R., Fuks, F., Shinkai, Y., Cedar, H., et al. (2008). De novo DNA methylation promoted by G9a prevents reprogramming of embryonically silenced genes. Nat Struct Mol Biol 15, 1176-1183. Esteller, M. (2007). Cancer epigenomics: DNA methylomes and histone-modification maps. Nat Rev Genet 8, 286-298. Feldman, N., Gerson, A., Fang, J., Li, E., Zhang, Y., Shinkai, Y., Cedar, H., and Bergman, Y. (2006). G9a-mediated irreversible epigenetic inactivation of Oct-3/4 during early embryogenesis. Nat Cell Biol 8, 188-194. Gerrard, L., Zhao, D., Clark, A.J., and Cui, W. (2005). Stably transfected human embryonic stem cell clones express OCT4-specific green fluorescent protein and maintain self-renewal and pluripotency. Stem Cells 23, 124-133. Gidekel, S., and Bergman, Y. (2002). A unique developmental pattern of Oct-3/4 DNA methylation is controlled by a cis-demodification element. J Biol Chem 277, 34521-34530. Gonzalez, B., Denzel, S., Mack, B., Conrad, M., and Gires, O. (2009). EpCAM is involved in maintenance of the murine embryonic stem cell phenotype. Stem Cells 27, 1782-1791. He, T.C., Sparks, A.B., Rago, C., Hermeking, H., Zawel, L., da Costa, L.T., Morin, P.J., Vogelstein, B., and Kinzler, K.W. (1998). Identification of c-MYC as a target of the APC pathway. Science 281, 1509-1512. High, A.S., Robinson, P.A., and Klein, C.E. (1996). Increased expression of a 38kd cell-surface glycoprotein MH99 (KS 1/4) in oral mucosal dysplasias. J Oral Pathol Med 25, 10-13. Huttner, H.B., Janich, P., Kohrmann, M., Jaszai, J., Siebzehnrubl, F., Blumcke, I., Suttorp, M., Gahr, M., Kuhnt, D., Nimsky, C., et al. (2008). The stem cell marker prominin-1/CD133 on membrane particles in human cerebrospinal fluid offers novel approaches for studying central nervous system disease. Stem Cells 26, 698-705. Jaenisch, R., and Young, R. (2008). Stem cells, the molecular circuitry of pluripotency and nuclear reprogramming. Cell 132, 567-582. Jones, D.L., and Wagers, A.J. (2008). No place like home: anatomy and function of the stem cell niche. Nat Rev Mol Cell Biol 9, 11-21. Jovic, M., Naslavsky, N., Rapaport, D., Horowitz, M., and Caplan, S. (2007). EHD1 regulates beta1 integrin endosomal transport: effects on focal adhesions, cell spreading and migration. J Cell Sci 120, 802-814. Kannagi, R., Cochran, N.A., Ishigami, F., Hakomori, S., Andrews, P.W., Knowles, B.B., and Solter, D. (1983). Stage-specific embryonic antigens (SSEA-3 and -4) are epitopes of a unique globo-series ganglioside isolated from human teratocarcinoma cells. EMBO J 2, 2355-2361. Kingston, R.E., and Narlikar, G.J. (1999). ATP-dependent remodeling and acetylation as regulators of chromatin fluidity. Genes Dev 13, 2339-2352. Knoepfler, P.S., Zhang, X.Y., Cheng, P.F., Gafken, P.R., McMahon, S.B., and Eisenman, R.N. (2006). Myc influences global chromatin structure. EMBO J 25, 2723-2734. Kolle, G., Ho, M., Zhou, Q., Chy, H.S., Krishnan, K., Cloonan, N., Bertoncello, I., Laslett, A.L., and Grimmond, S.M. (2009). Identification of human embryonic stem cell surface markers by combined membrane-polysome translation state array analysis and immunotranscriptional profiling. Stem Cells 27, 2446-2456. Kouzarides, T. (2007). Chromatin modifications and their function. Cell 128, 693-705. Kuroda, T., Tada, M., Kubota, H., Kimura, H., Hatano, S.Y., Suemori, H., Nakatsuji, N., and Tada, T. (2005). Octamer and Sox elements are required for transcriptional cis regulation of Nanog gene expression. Mol Cell Biol 25, 2475-2485. Lee, T.I., Jenner, R.G., Boyer, L.A., Guenther, M.G., Levine, S.S., Kumar, R.M., Chevalier, B., Johnstone, S.E., Cole, M.F., Isono, K., et al. (2006). Control of developmental regulators by Polycomb in human embryonic stem cells. Cell 125, 301-313. Litvinov, S.V., Balzar, M., Winter, M.J., Bakker, H.A., Briaire-de Bruijn, I.H., Prins, F., Fleuren, G.J., and Warnaar, S.O. (1997). Epithelial cell adhesion molecule (Ep-CAM) modulates cell-cell interactions mediated by classic cadherins. J Cell Biol 139, 1337-1348. Litvinov, S.V., van Driel, W., van Rhijn, C.M., Bakker, H.A., van Krieken, H., Fleuren, G.J., and Warnaar, S.O. (1996). Expression of Ep-CAM in cervical squamous epithelia correlates with an increased proliferation and the disappearance of markers for terminal differentiation. Am J Pathol 148, 865-875. Litvinov, S.V., Velders, M.P., Bakker, H.A., Fleuren, G.J., and Warnaar, S.O. (1994). Ep-CAM: a human epithelial antigen is a homophilic cell-cell adhesion molecule. J Cell Biol 125, 437-446. Loh, Y.H., Wu, Q., Chew, J.L., Vega, V.B., Zhang, W., Chen, X., Bourque, G., George, J., Leong, B., Liu, J., et al. (2006). The Oct4 and Nanog transcription network regulates pluripotency in mouse embryonic stem cells. Nat Genet 38, 431-440. Lu, T.Y., Kao, C.F., Lin, C.T., Huang, D.Y., Chiu, C.Y., Huang, Y.S., and Wu, H.C. (2009). DNA methylation and histone modification regulate silencing of OPG during tumor progression. J Cell Biochem 108, 315-325. Maetzel, D., Denzel, S., Mack, B., Canis, M., Went, P., Benk, M., Kieu, C., Papior, P., Baeuerle, P.A., Munz, M., et al. (2009). Nuclear signalling by tumour-associated antigen EpCAM. Nat Cell Biol 11, 162-171. Mermoud, J.E., Costanzi, C., Pehrson, J.R., and Brockdorff, N. (1999). Histone macroH2A1.2 relocates to the inactive X chromosome after initiation and propagation of X-inactivation. J Cell Biol 147, 1399-1408. Meshorer, E., Yellajoshula, D., George, E., Scambler, P.J., Brown, D.T., and Misteli, T. (2006). Hyperdynamic plasticity of chromatin proteins in pluripotent embryonic stem cells. Dev Cell 10, 105-116. Mikkelsen, T.S., Ku, M., Jaffe, D.B., Issac, B., Lieberman, E., Giannoukos, G., Alvarez, P., Brockman, W., Kim, T.K., Koche, R.P., et al. (2007). Genome-wide maps of chromatin state in pluripotent and lineage-committed cells. Nature 448, 553-560. Mitsui, K., Tokuzawa, Y., Itoh, H., Segawa, K., Murakami, M., Takahashi, K., Maruyama, M., Maeda, M., and Yamanaka, S. (2003). The homeoprotein Nanog is required for maintenance of pluripotency in mouse epiblast and ES cells. Cell 113, 631-642. Muncan, V., Sansom, O.J., Tertoolen, L., Phesse, T.J., Begthel, H., Sancho, E., Cole, A.M., Gregorieff, A., de Alboran, I.M., Clevers, H., et al. (2006). Rapid loss of intestinal crypts upon conditional deletion of the Wnt/Tcf-4 target gene c-Myc. Mol Cell Biol 26, 8418-8426. Munz, M., Baeuerle, P.A., and Gires, O. (2009). The emerging role of EpCAM in cancer and stem cell signaling. Cancer Res 69, 5627-5629. Munz, M., Kieu, C., Mack, B., Schmitt, B., Zeidler, R., and Gires, O. (2004). The carcinoma-associated antigen EpCAM upregulates c-myc and induces cell proliferation. Oncogene 23, 5748-5758. Mutskov, V., and Felsenfeld, G. (2004). Silencing of transgene transcription precedes methylation of promoter DNA and histone H3 lysine 9. EMBO J 23, 138-149. Nakatake, Y., Fukui, N., Iwamatsu, Y., Masui, S., Takahashi, K., Yagi, R., Yagi, K., Miyazaki, J., Matoba, R., Ko, M.S., et al. (2006). Klf4 cooperates with Oct3/4 and Sox2 to activate the Lefty1 core promoter in embryonic stem cells. Mol Cell Biol 26, 7772-7782. Ng, V.Y., Ang, S.N., Chan, J.X., and Choo, A.B. (2009). Characterization of Epithelial Cell Adhesion Molecule as a Surface Marker on Undifferentiated Human Embryonic Stem Cells. Stem Cells. Nichols, J., Zevnik, B., Anastassiadis, K., Niwa, H., Klewe-Nebenius, D., Chambers, I., Scholer, H., and Smith, A. (1998). Formation of pluripotent stem cells in the mammalian embryo depends on the POU transcription factor Oct4. Cell 95, 379-391. Niwa, H., Miyazaki, J., and Smith, A.G. (2000). Quantitative expression of Oct-3/4 defines differentiation, dedifferentiation or self-renewal of ES cells. Nat Genet 24, 372-376. Osta, W.A., Chen, Y., Mikhitarian, K., Mitas, M., Salem, M., Hannun, Y.A., Cole, D.J., and Gillanders, W.E. (2004). EpCAM is overexpressed in breast cancer and is a potential target for breast cancer gene therapy. Cancer Res 64, 5818-5824. Park, I.H., Zhao, R., West, J.A., Yabuuchi, A., Huo, H., Ince, T.A., Lerou, P.H., Lensch, M.W., and Daley, G.Q. (2008). Reprogramming of human somatic cells to pluripotency with defined factors. Nature 451, 141-146. Pasini, D., Bracken, A.P., Jensen, M.R., Lazzerini Denchi, E., and Helin, K. (2004). Suz12 is essential for mouse development and for EZH2 histone methyltransferase activity. EMBO J 23, 4061-4071. Reya, T., and Clevers, H. (2005). Wnt signalling in stem cells and cancer. Nature 434, 843-850. Reya, T., Duncan, A.W., Ailles, L., Domen, J., Scherer, D.C., Willert, K., Hintz, L., Nusse, R., and Weissman, I.L. (2003). A role for Wnt signalling in self-renewal of haematopoietic stem cells. Nature 423, 409-414. Rietze, R.L., Valcanis, H., Brooker, G.F., Thomas, T., Voss, A.K., and Bartlett, P.F. (2001). Purification of a pluripotent neural stem cell from the adult mouse brain. Nature 412, 736-739. Rodda, D.J., Chew, J.L., Lim, L.H., Loh, Y.H., Wang, B., Ng, H.H., and Robson, P. (2005). Transcriptional regulation of nanog by OCT4 and SOX2. J Biol Chem 280, 24731-24737. Rowland, B.D., Bernards, R., and Peeper, D.S. (2005). The KLF4 tumour suppressor is a transcriptional repressor of p53 that acts as a context-dependent oncogene. Nat Cell Biol 7, 1074-1082. Sato, N., Meijer, L., Skaltsounis, L., Greengard, P., and Brivanlou, A.H. (2004). Maintenance of pluripotency in human and mouse embryonic stem cells through activation of Wnt signaling by a pharmacological GSK-3-specific inhibitor. Nat Med 10, 55-63. Schipper, J.H., Frixen, U.H., Behrens, J., Unger, A., Jahnke, K., and Birchmeier, W. (1991). E-cadherin expression in squamous cell carcinomas of head and neck: inverse correlation with tumor dedifferentiation and lymph node metastasis. Cancer Res 51, 6328-6337. Schmelzer, E., Wauthier, E., and Reid, L.M. (2006). The phenotypes of pluripotent human hepatic progenitors. Stem Cells 24, 1852-1858. Schmelzer, E., Zhang, L., Bruce, A., Wauthier, E., Ludlow, J., Yao, H.L., Moss, N., Melhem, A., McClelland, R., Turner, W., et al. (2007). Human hepatic stem cells from fetal and postnatal donors. J Exp Med 204, 1973-1987. Schuettengruber, B., Chourrout, D., Vervoort, M., Leblanc, B., and Cavalli, G. (2007). Genome regulation by polycomb and trithorax proteins. Cell 128, 735-745. Schwartz, Y.B., and Pirrotta, V. (2007). Polycomb silencing mechanisms and the management of genomic programmes. Nat Rev Genet 8, 9-22. Sen, G.L., Webster, D.E., Barragan, D.I., Chang, H.Y., and Khavari, P.A. (2008). Control of differentiation in a self-renewing mammalian tissue by the histone demethylase JMJD3. Genes Dev 22, 1865-1870. Shackleton, M., Vaillant, F., Simpson, K.J., Stingl, J., Smyth, G.K., Asselin-Labat, M.L., Wu, L., Lindeman, G.J., and Visvader, J.E. (2006). Generation of a functional mammary gland from a single stem cell. Nature 439, 84-88. Shelby, R.D., Vafa, O., and Sullivan, K.F. (1997). Assembly of CENP-A into centromeric chromatin requires a cooperative array of nucleosomal DNA contact sites. J Cell Biol 136, 501-513. Shiah, S.G., Chang, L.C., Tai, K.Y., Lee, G.H., Wu, C.W., and Shieh, Y.S. (2009). The involvement of promoter methylation and DNA methyltransferase-1 in the regulation of EpCAM expression in oral squamous cell carcinoma. Oral Oncol 45, e1-8. Spizzo, G., Gastl, G., Obrist, P., Fong, D., Haun, M., Grunewald, K., Parson, W., Eichmann, C., Millinger, S., Fiegl, H., et al. (2007). Methylation status of the Ep-CAM promoter region in human breast cancer cell lines and breast cancer tissue. Cancer Lett 246, 253-261. Stingl, J., Raouf, A., Emerman, J.T., and Eaves, C.J. (2005). Epithelial progenitors in the normal human mammary gland. J Mammary Gland Biol Neoplasia 10, 49-59. Sundberg, M., Jansson, L., Ketolainen, J., Pihlajamaki, H., Suuronen, R., Skottman, H., Inzunza, J., Hovatta, O., and Narkilahti, S. (2009). CD marker expression profiles of human embryonic stem cells and their neural derivatives, determined using flow-cytometric analysis, reveal a novel CD marker for exclusion of pluripotent stem cells. Stem Cell Res 2, 113-124. Suto, R.K., Clarkson, M.J., Tremethick, D.J., and Luger, K. (2000). Crystal structure of a nucleosome core particle containing the variant histone H2A.Z. Nat Struct Biol 7, 1121-1124. Swigut, T., and Wysocka, J. (2007). H3K27 demethylases, at long last. Cell 131, 29-32. Tai, K.Y., Shiah, S.G., Shieh, Y.S., Kao, Y.R., Chi, C.Y., Huang, E., Lee, H.S., Chang, L.C., Yang, P.C., and Wu, C.W. (2007). DNA methylation and histone modification regulate silencing of epithelial cell adhesion molecule for tumor invasion and progression. Oncogene 26, 3989-3997. Takahashi, K., Tanabe, K., Ohnuki, M., Narita, M., Ichisaka, T., Tomoda, K., and Yamanaka, S. (2007). Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell 131, 861-872. Takahashi, K., and Yamanaka, S. (2006). Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell 126, 663-676. Takes, R.P., Baatenburg de Jong, R.J., Schuuring, E., Hermans, J., Vis, A.A., Litvinov, S.V., and van Krieken, J.H. (1997). Markers for assessment of nodal metastasis in laryngeal carcinoma. Arch Otolaryngol Head Neck Surg 123, 412-419. Tandon, A.K., Clark, G.M., Chamness, G.C., and McGuire, W.L. (1990). Association of the 323/A3 surface glycoprotein with tumor characteristics and behavior in human breast cancer. Cancer Res 50, 3317-3321. Thomson, J.A., Itskovitz-Eldor, J., Shapiro, S.S., Waknitz, M.A., Swiergiel, J.J., Marshall, V.S., and Jones, J.M. (1998). Embryonic stem cell lines derived from human blastocysts. Science 282, 1145-1147. Trzpis, M., McLaughlin, P.M., de Leij, L.M., and Harmsen, M.C. (2007). Epithelial cell adhesion molecule: more than a carcinoma marker and adhesion molecule. Am J Pathol 171, 386-395. van de Wetering, M., Sancho, E., Verweij, C., de Lau, W., Oving, I., Hurlstone, A., van der Horn, K., Batlle, E., Coudreuse, D., Haramis, A.P., et al. (2002). The beta-catenin/TCF-4 complex imposes a crypt progenitor phenotype on colorectal cancer cells. Cell 111, 241-250. Vignali, M., Hassan, A.H., Neely, K.E., and Workman, J.L. (2000). ATP-dependent chromatin-remodeling complexes. Mol Cell Biol 20, 1899-1910. Wernig, M., Meissner, A., Foreman, R., Brambrink, T., Ku, M., Hochedlinger, K., Bernstein, B.E., and Jaenisch, R. (2007). In vitro reprogramming of fibroblasts into a pluripotent ES-cell-like state. Nature 448, 318-324. Winter, M.J., Nagelkerken, B., Mertens, A.E., Rees-Bakker, H.A., Briaire-de Bruijn, I.H., and Litvinov, S.V. (2003). Expression of Ep-CAM shifts the state of cadherin-mediated adhesions from strong to weak. Exp Cell Res 285, 50-58. Yamashita, T., Budhu, A., Forgues, M., and Wang, X.W. (2007). Activation of hepatic stem cell marker EpCAM by Wnt-beta-catenin signaling in hepatocellular carcinoma. Cancer Res 67, 10831-10839. Ying, Q.L., Nichols, J., Evans, E.P., and Smith, A.G. (2002). Changing potency by spontaneous fusion. Nature 416, 545-548. Yu, G., Zhang, X., Wang, H., Rui, D., Yin, A., Qiu, G., and He, Y. (2008). CpG island methylation status in the EpCAM promoter region and gene expression. Oncol Rep 20, 1061-1067. Yu, J., Hu, K., Smuga-Otto, K., Tian, S., Stewart, R., Slukvin, II, and Thomson, J.A. (2009). Human induced pluripotent stem cells free of vector and transgene sequences. Science 324, 797-801. Zoller, M. (2009). Tetraspanins: push and pull in suppressing and promoting metastasis. Nat Rev Cancer 9, 40-55. Zorzos, J., Zizi, A., Bakiras, A., Pectasidis, D., Skarlos, D.V., Zorzos, H., Elemenoglou, J., and Likourinas, M. (1995). Expression of a cell surface antigen recognized by the monoclonal antibody AUA1 in bladder carcinoma: an immunohistochemical study. Eur Urol 28, 251-254. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/44954 | - |
dc.description.abstract | 人類胚胎幹細胞 (簡稱為幹細胞) 為一群具多功能性的細胞,能自我更新並分化成三個胚層。到目前為止,仍須有更多明確的表面標記以用來鑑別幹細胞。上皮細胞黏著分子 (EpCAM) 為第一型穿膜醣蛋白,表達在一些前驅細胞或癌細胞,也曾被用來純化具癌症起始能力之癌細胞 (tumor-initiating cells)。本研究中,我們利用一株對抗EpCAM 單株抗體OC98-1,透過免疫螢光顯微、西方點漬法與流式細胞分析廣泛地研究EpCAM 的表現。我們發現EpCAM 高度專一地表達在未分化的幹細胞,而不表現在已分化的細胞中。其蛋白質與轉錄體 (transcript) 在幹細胞進行分化時即減少。此現象之背後調控機制與轉換組織蛋白 (histone) 的後修飾作用緊密相關。進一步地,我們證明兩個調控組織蛋白3離氨酸27 三甲基化 (lysine 27 trimethylation of histone 3 ; H3K27me3) 的分子,SUZ12 與JMJD3,可作用在EpCAM 起始子上。過去研究發現SUZ12 與JMJD3可維持幹細胞多功能性之恆定。此外,我們也利用核染質免疫沈澱分析 (chromatin immunoprecipitation; ChIP) 證明EpCAM 可直接調控數個改編基因 (reprogramming gene),包括c-MYC、OCT-4、NANOG、SOX2 及KLF4,以利於維持幹細胞未分化的狀態。綜合以上結果,我們認為EpCAM 可作為幹細胞的表面標記,而EpCAM 在幹細胞內的表現,是透過表觀遺傳機制 (epigenetic mechanism) 所調控,並與維持幹細胞未分化特性緊密相關。 | zh_TW |
dc.description.abstract | Human embryonic stem cells (hESCs) are pluripotent cells capable of selfrenewal and differentiation into all three germ layers. To date, more cell surface markers capable of reliably identifying hESCs are needed. The epithelial cell adhesion molecule (EpCAM) is a type I transmembrane glycoprotein expressed in several progenitor cell populations and cancers. It has been used to enrich cells with tumorinitiating activity in xenograft transplantation studies. Here, we comprehensively profile the expression of EpCAM by immunofluorescence microscopy, Western blotting, and flow cytometry using an anti-EpCAM monoclonal antibody (MAb) OC98-1. We found EpCAM to be highly and selectively expressed by undifferentiated rather than differentiated hESCs. The protein and transcript level of EpCAM rapidly diminished as soon as hESC had differentiated. This silencing was closely and exclusively associated with the radical transformation of histone modification at the EpCAM promoter. Moreover, we demonstrated that the dynamic pattern of lysine 27 trimethylation of histone 3 (H3K27me3) was conferred by the interplay of SUZ12 and JMJD3, both of which were involved in maintaining hESC pluripotency. In addition, we used chromatin immunoprecipitation (ChIP) analysis to elucidate EpCAM’s direct regulation of several reprogramming genes, including c-MYC, OCT-4, NANOG, SOX2and KLF4, to help maintain the undifferentiation of hESCs. Collectively, our results suggest that EpCAM might be used as a surface marker for hESC. The expression of EpCAM may be regulated by epigenetic mechanisms, and it is strongly associated with the maintenance of the undifferentiated state of hESCs. | en |
dc.description.provenance | Made available in DSpace on 2021-06-15T03:59:22Z (GMT). No. of bitstreams: 1 ntu-99-F90444001-1.pdf: 20305487 bytes, checksum: 820dad8809ac60fad58398f87ae03564 (MD5) Previous issue date: 2010 | en |
dc.description.tableofcontents | PART 1. EpCAM regulation is associated with the maintenance of the undifferentiated phenotype of human embryonic stem cells…………………………………………………………………….1
中文摘要………………………………………………………………..2 Abstract………………………………………………………………..3 Chapter 1. Introduction…………………………………………….9 Chapter 2. Materials and Methods……………………………….23 Chapter 3. Results………………………………………………….32 Chapter 4. Discussion……………………………………………..43 References…………………………………………………………….50 Tables………………………………………………………………….62 Figures………………………………………………………………..63 PART 2. DNA methylation and histone modification silencing of OPG during tumor progression………………………………..76 中文摘要……………………………………………………………….77 Abstract……………………………………………………………….78 Chapter 1. Introduction…………………………………………..85 Chapter 2. Materials and Methods……………………………...95 Chapter 3. Results………………………………………………..109 Chapter 4. Discussion…………………………………………….120 References…………………………………………………………..127 Tables………………………………………………………………..134 Figures……………………………………………………………...137 | |
dc.language.iso | en | |
dc.title | EpCAM 調控人類胚胎幹細胞之未分化表態之研究 | zh_TW |
dc.title | EpCAM regulation is associated with maintenance of the
undifferentiated phenotype of human embryonic stem cells | en |
dc.type | Thesis | |
dc.date.schoolyear | 98-2 | |
dc.description.degree | 博士 | |
dc.contributor.oralexamcommittee | 游正博(John Yu),高承福(Cheng-Fu Kao),林中梧(Chung-Wu Lin),沈家寧(Chia-Ning Shen),呂 仁(Joyce Jean Lu) | |
dc.subject.keyword | 人類,胚胎幹細胞,EpCAM,細胞表面標記,表觀遺傳調控, | zh_TW |
dc.subject.keyword | human embryonic stem cell,EpCAM,cell surface marker,epigenetic regulation, | en |
dc.relation.page | 152 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2010-04-12 | |
dc.contributor.author-college | 醫學院 | zh_TW |
dc.contributor.author-dept | 病理學研究所 | zh_TW |
顯示於系所單位: | 病理學科所 |
文件中的檔案:
檔案 | 大小 | 格式 | |
---|---|---|---|
ntu-99-1.pdf 目前未授權公開取用 | 19.83 MB | Adobe PDF |
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。