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標題: | 高分子修飾之酵素電極於高選擇性電化學感測器之研究 Study of Highly Selective Electrochemical Sensors based on Polymer-modified Enzyme Electrodes |
作者: | Po-Chin Nien 粘博欽 |
指導教授: | 何國川 |
關鍵字: | 生物燃料電池,生物感測器,酵素,干擾物,葡萄糖,界面活性劑, biofuel cell,biosensor,enzyme,interference,glucose,surfactant., |
出版年 : | 2010 |
學位: | 博士 |
摘要: | 本論文主要目的是發展高選擇性生物感測器和探討其電化學特性。
首先,我們使用鍵結酵素固定法(第三章)來固定膽固醇氧化酵素在高分子Poly(3-thiopheneacetic acid)上。在定電流1.5 mA不同時間下,所得的三個紅橘色薄膜中,施加0.7 V (vs. Ag/AgCl/sat’d KCl)感測膽固醇得最好的靈敏度和偵測下限分別為4.49 mA M-1 cm-2和0.42 mM。干擾物抗壞血酸和尿酸則會分別增加原本訊號的5.2%和10.3%。另外一個方法固定酵素(第四章),則是利用高分子Poly(3,4- ethylenedioxythiophene) (PEDOT)來當固定網,以循環伏安法捕捉葡萄糖氧化酵素,此葡萄糖感測器的偵測下限和靈敏度分別為0.13 mM 和12.42 mA cm-2 M-1。在干擾物抗壞血酸和尿酸方面,感測訊號則增加了9.7%和39.1%。 為了更進一步降低干擾,本研究提出由兩個工作電極(WE1和WE2)組成之微電極陣列(第五章),其中WE1是以圈鍍法製備的酵素修飾電極,WE2則是白金電極,在液體流經WE1之前,用以氧化在邊界層中的干擾物。在流動系統中,施加0.7 V於WE1來偵測過氧化氫,感測葡萄糖之靈敏度為157 The main purpose of this dissertation is to develop a highly selective biosensor and discuss its electrochemical properties. First, the covalent enzyme immobilization method (in Chapter 3) was proposed to attach cholesterol oxidase (ChO) on poly(3-thiopheneacetic acid). Three different red-orange films were electropolymerized by applying 1.5 mA for different times. The best sensitivity (SEN) for cholesterol obtained is 4.49 mA M-1 cm-2 at 0.7 V (vs. Ag/AgCl/sat’d KCl) and the limit of detection (LOD) is 0.42 mM. The interferences, ascorbic acid (AA) and uric acid (UA), increased 5.2% and 10.3% of the original value, respectively. By another method (in Chapter 4) to immobilize enzyme, poly(3,4- ethylenedioxythiophene) (PEDOT) is used as a matrix for entrapping glucose oxidase (GOD) by the cyclic voltammetry (CV). The LOD and SEN on glucose for the biosensor are 0.13 mM and 12.42 mA cm-2 M-1, respectively. In the aspects of interferences on AA and UA, the sensing currents increased about 9.7 % and 39.1 %, respectively. For lowering the interference further, the inter-digitated array microelectrodes (in Chapter 5) consist of two working electrodes (WE1 & WE2), in which WE1 was the enzyme-modified electrode immobilized by entrapment and WE2 was a platinum to oxidize the interference near the boundary layer before the stream reaching WE1. In the flow system, 0.7 V was applied on WE1 to detect H2O2. The SEN for glucose was 157 |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/44904 |
全文授權: | 有償授權 |
顯示於系所單位: | 化學工程學系 |
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