請用此 Handle URI 來引用此文件:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/44697
完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 黃鵬鵬(Pung-Pung Hwang) | |
dc.contributor.author | Hao-Hsuan Hsu | en |
dc.contributor.author | 徐浩軒 | zh_TW |
dc.date.accessioned | 2021-06-15T03:53:06Z | - |
dc.date.available | 2013-07-12 | |
dc.date.copyright | 2010-07-12 | |
dc.date.issued | 2010 | |
dc.date.submitted | 2010-07-05 | |
dc.identifier.citation | 參考文獻
Al-Awqati Q. 2003. Terminal differentiation of intercalated cells: the role of hensin. Annu Rev Physiol 65: 567. Avella M and Bornancin M. 1989. A new analysis of ammonia and sodium transport through the gills of the freshwater rainbow trout, salmo gairdneri. J Exp Biol 142 (1): 155. Bagnis C, Marshansky V, Breton S and Brown D. 2001. Remodeling the cellular profile of collecting ducts by chronic carbonic anhydrase inhibition. Am J Physiol Renal Physiol 280 (3): F437. Chang IC, Lee TH, Yang CH, Wei YY, Chou FI and Hwang PP. 2001. Morphology and function of gill mitochondria-rich cells in fish acclimated to different environments. Physiol Biochem Zool 74 (1): 111. Chang IC, Wei YY, Chou FI and Hwang PP. 2003. Stimulation of Cl--uptake and morphological changes in gill mitochondria-rich cells in freshwater tilapia, Oreochromis mossambicus. Physiol Biochem Zool 76 (4): 544. Chang WJ, Horng JL, Yan JJ, Hsiao CD and Hwang PP. 2009. The transcription factor, glial cell missing 2, is involved in differentiation and functional regulation of H+-ATPase-rich cells in zebrafish, Danio rerio. Am J Physiol Regul Integr Comp Physiol 296 (4): R1192. Chretien M and Pisam M. 1986. Cell renewal and differentiation in the gill epithelium of fresh- or salt-water adapted euryhaline fish as revealed by 3H-thymidine autoradiography. Biol Cell 56 (2): 137. Cutler CP and Cramb G. 2002. Two isoforms of the Na+/K+/2Cl- cotransporter are expressed in the European eel, Anguilla anguilla. Biochim Biophys Acta 1566: 92. Esaki M, Hoshijima K, Kobayashi S, Fukuda H, Kawakami K and Hirose S. 2007. Visualization in zebrafish larvae of Na+ uptake in mitochondria-rich cells whose differentiation is dependent on foxi3a. Am J Physiol Regul Integr Comp Physiol 292 (1): R470. Evans DH, Hoar WS and Randall DJ. The roles of gill permeability and transport mechanisms in euryhalinity. Fish Physiology, (Academic Press, 1984). 10 (Pt 2): 239. Evans DH, Piermarini PM and Choe KP. 2005. The multifunctional fish gill: dominant site of gas exchange, osmoregulation, acid-base regulation, and excretion of nitrogenous waste. Physiol Rev 85 (1): 97. Galvez F, Reid SD, Hawkings G and Goss GG. 2002. Isolation and characterization of mitochondria-rich cell types from the gill of freshwater rainbow trout. Am J Physiol Regul Integr Comp Physiol 282 (3): R658. Goss GG, Adamia S and Galvez F. 2001. Peanut lectin binds to a subpopulation of mitochondria-rich cells in the rainbow trout gill epithelium. Am J Physiol Regul Integr Comp Physiol 281 (5): R1718. Hikita C, Takito J, Vijayakumar S and Al-Awqati Q. 1999. Only multimeric hensin located in the extracellular matrix can induce apical endocytosis and reverse the polarity of intercalated cells. J Biol Chem 274 (25): 17671. Hikita C, Vijayakumar S, Takito J, Erdjument-Bromage H, Tempst P and Al-Awqati Q. 2000. Induction of terminal differentiation in epithelial cells requires polymerization of hensin by galectin3. J Cell Biol 151 (6): 1235. Hirata T, Kaneko T, Ono T, Nakazato T, Furukawa N, Hasegawa S et al. 2003. Mechanism of acid adaptation of a fish living in a pH 3.5 lake. Am J Physiol Regul Integr Comp Physiol 284 (5): R1199. Hiroi J, Kaneko T and Tanaka M. 1999. In vivo sequential changes in chloride cell morphology in the yolk-sac membrane of mozambique tilapia, Oreochromis mossambicus, embryos and larvae during seawater adaptation. J Exp Biol 202 (Pt 24): 3485. Hiroi J and McCormick SD. 2007. Variation in salinity tolerance, gill Na+/K+-ATPase, Na+/K+/2Cl- cotransporter and mitochondria-rich cell distribution in three salmonids Salvelinus namaycush, Salvelinus fontinalis and Salmo salar. J Exp Biol 210 (Pt 6): 1015. Hiroi J, McCormick SD, Ohtani-Kaneko R and Kaneko T. 2005. Functional classification of mitochondrion-rich cells in euryhaline Mozambique tilapia, Oreochromis mossambicus, embryos by means of triple immunofluorescence staining for Na+/K+-ATPase, Na+/K+/2Cl- cotransporter and CFTR anion channel. J Exp Biol 208 (Pt 11): 2023. Hiroi J, Yasumasu S, McCormick SD, Hwang PP and Kaneko T. 2008. Evidence for an apical Na+/Cl--cotransporter involved in ion uptake in a teleost fish. J Exp Biol 211 (Pt 16): 2584. Horng JL, Lin LY and Hwang PP. 2009. Functional regulation of H+-ATPase-rich cells in zebrafish embryos acclimated to an acidic environment. Am J Physiol Cell Physiol 296 (4): C682. Hsiao CD, You MS, Guh YJ, Ma M, Jiang YJ and Hwang PP. 2007. A positive regulatory loop between foxi3a and foxi3b is essential for specification and differentiation of zebrafish epidermal ionocytes. Plos One 2 (3): e302. Hwang PP. 2009. Ion uptake and acid secretion in zebrafish, Danio rerio. J Exp Biol 212 (Pt 11): 1745. Hwang PP and Lee TH. 2007. New insights into fish ion regulation and mitochondrion-rich cells. Comp Biochem Physiol A Mol Integr Physiol 148 (3): 479. Hwang PP, Perry SF and Colin JB. Ionic and acid-base regulation. Fish Physiology, (Academic Press, 2010). 29: 311. Inokuchi M, Hiroi J, Watanabe S, Hwang PP and Kaneko T. 2009. Morphological and functional classification of ion-absorbing mitochondria-rich cells in the gills of Mozambique tilapia. J Exp Biol 212 (Pt 7): 1003. Inokuchi M, Hiroi J, Watanabe S, Lee KM and Kaneko T. 2008. Gene expression and morphological localization of NHE3, NCC and NKCC1a in branchial mitochondria-rich cells of Mozambique tilapia, Oreochromis mossambicus, acclimated to a wide range of salinities. Comp Biochem Physiol A Mol Integr Physiol 151 (2): 151. Ivanis G, Braun M and Perry SF. 2008. Renal expression and localization of SLC9A3 sodium/hydrogen exchanger and its possible role in acid-base regulation in freshwater rainbow trout, Oncorhynchus mykiss. Am J Physiol Regul Integr Comp Physiol 295 (3): R971. Janicke M, Carney TJ and Hammerschmidt M. 2007. Foxi3 transcription factors and Notch signaling control the formation of skin ionocytes from epidermal precursors of the zebrafish embryo. Dev Biol 307 (2): 258. Kang CK, Tsai SC, Lee TH and Hwang PP. 2008. Differential expression of branchial Na+/K+-ATPase of two medaka species, Oryzias latipes and Oryzias dancena, with different salinity tolerances acclimated to fresh water, brackish water and seawater. Comp Biochem Physiol A Mol Integr Physiol 151 (4): 566. Katoh F and Kaneko T. 2003. Short-term transformation and long-term replacement of branchial chloride cells in killifish transferred from seawater to freshwater, revealed by morphofunctional observations and a newly established 'time-differential double fluorescent staining' technique. J Exp Biol 206 (Pt 22): 4113. Kirschner LB. 1983. Sodium chloride absorption across the body surface: frog skins and other epithelia. Am J Physiol 244 (4): R429. Lai YW. 2008. Expression and regulation of gill ion transporters in Japanese medaka, Oryzias latipes, during seawater acclimation. Unpublished master thesis, Graduate Institute of Biochemical Sciences, National Taiwan Univserty. Lee TH, Feng SH, Lin CH, Hwang YH, Huang CL and Hwang PP. 2003. Ambient salinity modulates the expression of sodium pumps in branchial mitochondria-rich cells of Mozambique tilapia, Oreochromis mossambicus. Zool Sci 20 (1): 29. Lee TH, Hwang PP, Lin HC and Huang FL. 1996. Mitochondria-rich cells in the branchial epithelium of the teleost,Oreochromis mossambicus, acclimated to various hypotonic environments. Fish Physiol Biochem 15 (6): 513. Liao BK, Deng AN, Chen SC, Chou MY and Hwang PP. 2007. Expression and water calcium dependence of calcium transporter isoforms in zebrafish gill mitochondrion-rich cells. BMC Genomics 8: 354. Lin CC. 2009. Molecular physiological study on Na+ uptake/acid-base regulation mechanisms in Japanese medaka, Oryzias latipes. Unpublished master thesis, Graduate Institute of Fisheries Sciences, National Taiwan University. Lin LY, Horng JL, Kunkel JG and Hwang PP. 2006. Proton pump-rich cell secretes acid in skin of zebrafish larvae. Am J Physiol Cell Physiol 290 (2): C371. Lin LY and Hwang PP. 2001. Modification of morphology and function of integument mitochondria-rich cells in tilapia larvae, Oreochromis mossambicus, acclimated to ambient chloride levels. Physiol Biochem Zool 74 (4): 469. Lytle C, Xu JC, Biemesderfer D and Forbush B, 3rd. 1995. Distribution and diversity of Na+/K+/2Cl--cotransport proteins: a study with monoclonal antibodies. Am J Physiol 269 (6 Pt 1): C1496. Marshall WS. 2002. Na+, Cl-, Ca2+ and Zn2+ transport by fish gills: retrospective review and prospective synthesis. J Exp Zool 293 (3): 264. Marshall WS, Lynch EM and Cozzi RR. 2002. Redistribution of immunofluorescence of CFTR anion channel and NKCC cotransporter in chloride cells during adaptation of the killifish Fundulus heteroclitus to seawater. J Exp Biol 205 (Pt 9): 1265. Mollenhauer J, Wiemann S, Scheurlen W, Korn B, Hayashi Y, Wilgenbus KK et al. 1997. DMBT1, a new member of the SRCR superfamily, on chromosome 10q25.3-26.1 is deleted in malignant brain tumours. Nat Genet 17 (1): 32. Pan TC, Liao BK, Huang CJ, Lin LY and Hwang PP. 2005. Epithelial Ca2+ channel expression and Ca2+ uptake in developing zebrafish. Am J Physiol Regul Integr Comp Physiol 289 (4): R1202. Schwartz GJ and Al-Awqati Q. 2005. Role of hensin in mediating the adaptation of the cortical collecting duct to metabolic acidosis. Curr Opin Nephrol Hypertens 14 (4): 383. Schwartz GJ, Tsuruoka S, Vijayakumar S, Petrovic S, Mian A and Al-Awqati Q. 2002. Acid incubation reverses the polarity of intercalated cell transporters, an effect mediated by hensin. J Clin Invest 109 (1): 89. Scott GR, Claiborne JB, Edwards SL, Schulte PM and Wood CM. 2005. Gene expression after freshwater transfer in gills and opercular epithelia of killifish: insight into divergent mechanisms of ion transport. J Exp Biol 208 (Pt 14): 2719. Scott GR, Richards JG, Forbush B, Isenring P and Schulte PM. 2004. Changes in gene expression in gills of the euryhaline killifish, Fundulus heteroclitus, after abrupt salinity transfer. Am J Physiol Cell Physiol 287 (2): C300. Shahsavarani A, McNeill B, Galvez F, Wood CM, Goss GG, Hwang PP et al. 2006. Characterization of a branchial epithelial calcium channel (ECaC) in freshwater rainbow trout, Oncorhynchus mykiss. J Exp Biol 209 (Pt 10): 1928. Takito J, Hikita C and Al-Awqati Q. 1996. Hensin, a new collecting duct protein involved in the in vitro plasticity of intercalated cell polarity. J Clin Invest 98 (10): 2324. Takito J, Yan L, Ma J, Hikita C, Vijayakumar S, Warburton D et al. 1999. Hensin, the polarity reversal protein, is encoded by DMBT1, a gene frequently deleted in malignant gliomas. Am J Physiol 277 (2 Pt 2): F277. Tang CH, Chiu YH, Tsai SC and Lee TH. 2009. Relative changes in the abundance of branchial Na+/K+-ATPase alpha-isoform-like proteins in marine euryhaline milkfish, Chanos chanos, acclimated to environments of different salinities. J Exp Zool A Ecol Genet Physiol 311 (7): 521. Tang CH and Lee TH. 2007. The effect of environmental salinity on the protein expression of Na+/K+-ATPase, Na+/K+/2Cl- cotransporter, cystic fibrosis transmembrane conductance regulator, anion exchanger 1, and chloride channel 3 in gills of a euryhaline teleost, Tetraodon nigroviridis. Comp Biochem Physiol A Mol Integr Physiol 147 (2): 521. Thermes V, Lin CC and Hwang PP. 2010. Expression of Ol-foxi3 and Na+/K+-ATPase in ionocytes during the development of euryhaline medaka, Oryzias latipes, embryos. Gene Expr Patterns 10(4-5):185. Tipsmark CK, Madsen SS and Borski RJ. 2004. Effect of salinity on expression of branchial ion transporters in striped bass, Morone saxatilis. J Exp Zool A Comp Exp Biol 301 (12): 979. Tipsmark CK, Madsen SS, Seidelin M, Christensen AS, Cutler CP and Cramb G. 2002. Dynamics of Na+/K+/2Cl--cotransporter and N+/K+-ATPase expression in the branchial epithelium of brown trout, Salmo trutta, and Atlantic salmon, Salmo salar. J Exp Zool 293 (2): 106. Uchida K and Kaneko T. 2009. Enhanced Chloride Cell Turnover in the Gills of Chum Salmon Fry in Seawater. Zool Sci 13 (5): 655. Wang PJ, Lin CH, Hwang LY, Huang CL, Lee TH and Hwang PP. 2009. Differential responses in gills of euryhaline tilapia, Oreochromis mossambicus, to various hyperosmotic shocks. Comp Biochem Physiol A Mol Integr Physiol 152 (4): 544. Wang YF, Tseng YC, Yan JJ, Hiroi J and Hwang PP. 2009. Role of SLC12A10.2, a Na+/Cl--cotransporter-like protein, in a Cl- uptake mechanism in zebrafish, Danio rerio. Am J Physiol Regul Integr Comp Physiol 296 (5): R1650. Wittbrodt J, Shima A and Schartl M. 2002. Medaka-a model organism from the far east. Nat Rev Genet 3 (1): 53. Wu SC, Horng JL, Liu ST, Hwang PP, Wen ZH, Lin CS et al. 2010. Ammonium-dependent sodium uptake in mitochondrion-rich cells of medaka, Oryzias latipes, larvae. Am J Physiol Cell Physiol 298 (2): C237. Wu YC, Lin LY and Lee TH. 2003. Na+/K+/2Cl--cotransporter: A Novel Marker for Identifying Freshwater- and Seawater-type Mitochondria-rich Cells in Gills of the Euryhaline Tilapia, Oreochromis mossambicus. Zool Stud 42 (1): 186. Yan JJ, Chou MY, Kaneko T and Hwang PP. 2007. Gene expression of Na+/H+-exchanger in zebrafish H+-ATPase-rich cells during acclimation to low-Na+ and acidic environments. Am J Physiol Cell Physiol 293 (6): C1814. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/44697 | - |
dc.description.abstract | 離子細胞或富含粒腺體細胞為一群特化之表皮細胞,能主動吸收或排出離子維持體液滲透壓平衡。本研究以日本種稻田魚 (青鱂魚) (Japanese Medaka, Oryzias latipes) 為模式動物,闡明廣鹽性硬骨魚類短期適應海水細胞及分子生理機制。
本研究將青鱂魚離子細胞歸類為四型,淡水有三型:HA細胞、NCC細胞及NHE細胞,海水只有一型SW細胞。NHE細胞占所有NKA免疫反應性離子細胞絕大多數,頂膜表現鈉氫交換蛋白 (Na+/H+ exchanger-3, NHE3)、氯離子通道 (cystic fibrosis conductance regulator, CFTR)、基側膜表現鈉鉀幫浦 (Na+/K+-ATPase, NKA) 及鈉鉀氯共同運輸蛋白 (Na+/K+/2Cl- cotransporter-1a, NKCC1a)。NCC細胞較少數,頂膜表現 (Na+/Cl- cotransporter, NCC)。 海水轉移適應過程中,上述離子運輸蛋白表現量產生對應消長。在海水適應青鱂魚胚胎表皮NKCC1a顯著增加而NCC不表現。成魚轉移海水實驗當中,鰓上NHE3基因表現量下降但是CFTR基因表現量上升。我們以NKA標定青鱂魚胚胎離子細胞,發現轉移過程當中離子細胞密度不變,而細胞體積變大。 短期轉移海水過程中,青鱂魚胚胎表皮P63幹細胞標密度在1、7小時無顯著變化,至1天則有些許下降。TUNEL assay染色結果顯示表皮細胞凋亡作用無顯著變化。離子細胞分化相關轉錄因子foxi3與gcm2表現量在海水轉移24小時皆無顯著差異,這些實驗結果顯示青鱂魚胚胎表皮細胞新生、分化及死亡作用在短期轉移海水變化皆不顯著。 DMBT1為哺乳類Hensin同源蛋白,Hensin為促進腎臟閏細胞產生轉型作用的重要因子。我們發現青鱂魚DMBT1表現在離子細胞上,海水轉移過程中,DMBT1免疫染色反應性變低,然而基因層級表現變化不顯著。抑制dmbt1後再轉移海水則導致胚胎孵化延遲,我們推測dmbt1可能參與海水轉移適應過程中細胞轉型作用。 綜言之,本研究推測廣鹽性硬骨魚類短期適應海水時,表皮細胞更新作用無顯著變化,離子細胞的數目亦不變,但是離子細胞型態及運輸蛋白表現因應環境鹽度變化受到調控,淡水型離子細胞可能直接轉型為海水型離子細胞,而DMBT1可能參與短期海水轉移細胞轉型作用。 | zh_TW |
dc.description.abstract | Ionocytes, mitochondrion-rich cells (MRCs), are specialized epidermal cells which maintain ionic homeostasis of body fluid by actively taking up or excreting ions. The purpose of this study is to examine the regulatory mechanism of cell turnover and transporters expression of ionocytes in Japanese Medaka (Oryzias latipes) during seawater (SW) acclimation.
Four types of ionocyte were identified in medaka embryo, the freshwater (FW) type were NHE cell, NCC cell and HA cell and only one SW type ionocyte. The NHE cells expressing apical Na+/H+ exchanger-3 (NHE3), cystic fibrosis conductance regulator (CFTR), and basolateral Na+/K+-ATPase (NKA) and constituted the majority of FW ionocytes. We also found that most of ionocytes were basolateral expressing NKCC1a and co-localized with NKA suggesting that they are belonged to NHE cell. The cell number of NCC cell expressing apical Na+/Cl- cotransporter (NCC) was in minority. Expressions of above-mentioned transporters were regulated in response to SW stimulation. NKCC1a signal was up-regulated and no apical NCC signal was found in SW-acclimated embryo. The gene expression of NHE3 (slc9a3) was down-regulated but CFTR (abcc7) was up-regulated in medaka gill during 24 hours SW transfer. In 2-days SW transfer medaka embryo, the density of NKA-immunoreactive ionocytes was not changed, but the cell size became larger. Ionocyte turnover was examined on medaka yolk sac membrane during short-term SW adaptation. P63 is an epidermal stem cell marker, immunocytochemistry of P63 showed no significant difference in the density of P63-cells after 1 hour and 7 hours SW transfer, but a little decrease showed after 1 day SW transfer. The result of TUNEL assay indicated that cell apoptosis was not significant at 12, 24 and 48 hours after SW transfer. By qRT-PCR, The expression of ionocyte differentiation marker foxi3 and gcm2 did not differ between FW and 24-hour SW transfer medaka gill. These results suggest that proliferation, differentiation and apoptosis of epidermal cells were not changed in short-term SW adaptation response. In mammalian, deleted in malignant brain tumor 1 (dmbt1) encodes Hensin which transform b-intercalated cell into a-intercalated cell in cortical collecting duct during systemic metabolic acidosis. In medaka, dmbt1 was also expressed in ionocytes. The protein expression of DMBT1 was down-regulated in 36 hours SW transfer embryo by immunocytochemistry. However, there was no difference in the gene expression of dmbt1 during 24 hours SW tranfer. Loss-of-function experiment showed higher mortality in dmbt1 morpholino-injected group than in control. Morpholino knock down accompany with SW transfer treatment showed obvious delay in the development of medaka morphant. We suggested dmbt1 might involve in transformation of ionocytes during SW acclimation. Taken together, we demonstrated a model of short-term SW adaptation in medaka which suggests that FW ionocytes directly transform to SW ionocytes and dmbt1 might involve in this process. | en |
dc.description.provenance | Made available in DSpace on 2021-06-15T03:53:06Z (GMT). No. of bitstreams: 1 ntu-99-R97b45003-1.pdf: 18430685 bytes, checksum: dbfa929f686efff48936a05fb4fc126a (MD5) Previous issue date: 2010 | en |
dc.description.tableofcontents | 口試委員會審定書...i
誌謝..............ii 摘要.............iii Abstract...........v 目錄.............vii 圖目錄..........viii 表目錄............ix 前言...............1 材料與方法.........9 結果..............13 討論..............16 參考文獻..........22 附圖..............33 附表..............47 附錄..............50 | |
dc.language.iso | zh-TW | |
dc.title | 日本種稻田魚適應海水之分子及細胞功能調控機制 | zh_TW |
dc.title | Molecular and cellular mechanisms of functional modification of ionocytes during seawater acclimation in Japanese Medaka (Oryzias latipes) | en |
dc.type | Thesis | |
dc.date.schoolyear | 98-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 林豊義,李宗翰,張清風,羅秀婉 | |
dc.subject.keyword | 青鱂,魚,離子細胞,海水,鈉氫離子交換蛋白,纖維性囊腫穿膜傳導調節蛋白,DMBT1, | zh_TW |
dc.subject.keyword | Japanese medaka,ionocytes,seawater,NHE3,CFTR,DMBT1, | en |
dc.relation.page | 51 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2010-07-05 | |
dc.contributor.author-college | 生命科學院 | zh_TW |
dc.contributor.author-dept | 漁業科學研究所 | zh_TW |
顯示於系所單位: | 漁業科學研究所 |
文件中的檔案:
檔案 | 大小 | 格式 | |
---|---|---|---|
ntu-99-1.pdf 目前未授權公開取用 | 18 MB | Adobe PDF |
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。