請用此 Handle URI 來引用此文件:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/44655
完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 楊台鴻 | |
dc.contributor.author | Wei-Ting Lin | en |
dc.contributor.author | 林韋廷 | zh_TW |
dc.date.accessioned | 2021-06-15T03:52:23Z | - |
dc.date.available | 2015-07-15 | |
dc.date.copyright | 2010-07-15 | |
dc.date.issued | 2010 | |
dc.date.submitted | 2010-07-08 | |
dc.identifier.citation | 參考文獻 Arase, S., Y. Sadamoto and S. Katoh (1991). 'Coculture of human hair follicles and dermal papillae in type I collagen gel.' Ann N Y Acad Sci 642: 454-456.
Baitsell, G. A. (1915). 'The Origin and Structure of a Fibrous Tissue Which Appears in Living Cultures of Adult Frog Tissues.' J Exp Med 21(5): 455-478. Botchkarev, V. A., N. V. Botchkareva, A. A. Sharov, K. Funa, et al. (2002). 'Modulation of BMP signaling by noggin is required for induction of the secondary (nontylotrich) hair follicles.' J Invest Dermatol 118(1): 3-10. Callen, J. P. (1982). 'Chronic cutaneous lupus erythematosus. Clinical, laboratory, therapeutic, and prognostic examination of 62 patients.' Arch Dermatol 118(6): 412-416. Campbell, C. E. and A. F. von Recum (1989). 'Microtopography and soft tissue response.' J Invest Surg 2(1): 51-74. Carpenedo, R. L., C. Y. Sargent and T. C. McDevitt (2007). 'Rotary suspension culture enhances the efficiency, yield, and homogeneity of embryoid body differentiation.' Stem Cells 25(9): 2224-2234. Cetin, E. D., E. Savk, M. Uslu, M. Eskin, et al. (2009). 'Investigation of the inflammatory mechanisms in alopecia areata.' Am J Dermatopathol 31(1): 53-60. Chase, H. B. (1954). 'Growth of the hair.' Physiol Rev 34(1): 113-126. Chen, C. H., C. C. Tsai, W. Chen, F. L. Mi, et al. (2006). 'Novel living cell sheet harvest system composed of thermoreversible methylcellulose hydrogels.' Biomacromolecules 7(3): 736-743. Chen, K. S., L. Tatarczuch, Y. Ahmed, H. H. Huang, et al. (2010). 'Identification of light and dark hypertrophic chondrocytes in mouse and rat chondrocyte pellet cultures.' Tissue Cell 42(2): 121-128. Chen, R. S., Y. J. Chen, M. H. Chen and T. H. Young (2009). 'The behavior of rat tooth germ cells on poly(vinyl alcohol).' Acta Biomater 5(4): 1064-1074. Cohen, J. (1961). 'The transplantation of individual rat and guineapig whisker papillae.' J Embryol Exp Morphol 9: 117-127. Cotsarelis, G., T. T. Sun and R. M. Lavker (1990). 'Label-retaining cells reside in the bulge area of pilosebaceous unit: implications for follicular stem cells, hair cycle, and skin carcinogenesis.' Cell 61(7): 1329-1337. Dang, S. M., M. Kyba, R. Perlingeiro, G. Q. Daley, et al. (2002). 'Efficiency of embryoid body formation and hematopoietic development from embryonic stem cells in different culture systems.' Biotechnol Bioeng 78(4): 442-453. Davies, K. (1998). 'Human genetics. Hair apparent.' Nature 391(6667): 537, 539. De Villez, R. L. (1985). 'Topical minoxidil therapy in hereditary androgenetic alopecia.' Arch Dermatol 121(2): 197-202. du Cros, D. L., R. G. LeBaron and J. R. Couchman (1995). 'Association of versican with dermal matrices and its potential role in hair follicle development and cycling.' J Invest Dermatol 105(3): 426-431. Evans, H. M., F. B. Bowman and M. C. Winternitz (1914). 'An Experimental Study of the Histogenesis of the Miliary Tubercle in Vitally Stained Rabbits.' J Exp Med 19(3): 283-302. Freshney, R. I. (2005). Culture of animal cells. Hoboken, N.J. , Wiley-Liss. Fuchs, E. (2007). 'Scratching the surface of skin development.' Nature 445(7130): 834-842. Garg, S. and A. G. Messenger (2009). 'Alopecia areata: evidence-based treatments.' Semin Cutan Med Surg 28(1): 15-18. Haque, A. and E. R. Morris (1993). 'Thermogelation of methylcellulose. Part I: molecular structures and processes.' Carbohydrate Polymers 22(3): 161-173. Hardy, M. H. (1992). 'The secret life of the hair follicle.' Trends Genet 8(2): 55-61. Harrison, R. G. (1907). 'Observations on the living developing nerve fiber.' Anatomical Record 1(5): 116-118. Harrison, R. G. (1910). 'The outgrowth of the nerve fiber as a mode of protoplasmic movement.' Journal of Experimental Zoology 9(4): 787-U727. Headington, J. T. (1993). 'Telogen effluvium. New concepts and review.' Arch Dermatol 129(3): 356-363. Headington, J. T. (1996). 'Cicatricial alopecia.' Dermatol Clin 14(4): 773-782. Hibberts, N. A., A. E. Howell and V. A. Randall (1998). 'Balding hair follicle dermal papilla cells contain higher levels of androgen receptors than those from non-balding scalp.' J Endocrinol 156(1): 59-65. Hiraizumi, Y., E. E. Transfeldt, E. Fujimaki and M. Nambu (1995). 'Application of polyvinyl alcohol hydrogel membrane as anti-adhesive interposition after spinal surgery.' Spine (Phila Pa 1976) 20(21): 2272-2277. Hoffmann, R. and R. Happle (1999). 'Finasteride is the main inhibitor of 5alpha-reductase activity in microdissected dermal papillae of human hair follicles.' Arch Dermatol Res 291(2-3): 100-103. Iio, K., N. Minoura, S. Aiba, M. Nagura, et al. (1994). 'Cell growth on poly(vinyl alcohol) hydrogel membranes containing biguanido groups.' J Biomed Mater Res 28(4): 459-462. Ito, M., Z. Yang, T. Andl, C. Cui, et al. (2007). 'Wnt-dependent de novo hair follicle regeneration in adult mouse skin after wounding.' Nature 447(7142): 316-320. Jahoda, C. A., K. A. Horne and R. F. Oliver (1984). 'Induction of hair growth by implantation of cultured dermal papilla cells.' Nature 311(5986): 560-562. Jahoda, C. A. and R. F. Oliver (1984). 'Vibrissa dermal papilla cell aggregative behaviour in vivo and in vitro.' J Embryol Exp Morphol 79: 211-224. Jahoda, C. A., A. J. Reynolds and R. F. Oliver (1993). 'Induction of hair growth in ear wounds by cultured dermal papilla cells.' J Invest Dermatol 101(4): 584-590. Keller, G. M. (1995). 'In vitro differentiation of embryonic stem cells.' Curr Opin Cell Biol 7(6): 862-869. Kishimoto, J., R. Ehama, L. Wu, S. Jiang, et al. (1999). 'Selective activation of the versican promoter by epithelial- mesenchymal interactions during hair follicle development.' Proc Natl Acad Sci U S A 96(13): 7336-7341. Kokkinos, M. I., R. Wafai, M. K. Wong, D. F. Newgreen, et al. (2007). 'Vimentin and epithelial-mesenchymal transition in human breast cancer--observations in vitro and in vivo.' Cells Tissues Organs 185(1-3): 191-203. Kurosawa, H. (2007). 'Methods for inducing embryoid body formation: in vitro differentiation system of embryonic stem cells.' J Biosci Bioeng 103(5): 389-398. Lee, W.-Y., Y.-H. Chang, Y.-C. Yeh, C.-H. Chen, et al. (2009). 'The use of injectable spherically symmetric cell aggregates self-assembled in a thermo-responsive hydrogel for enhanced cell transplantation.' Biomaterials 30(29): 5505-5513. Li, Y., G. Q. Li, C. M. Lin and X. N. Cai (2005). 'One-step collagenase I treatment: an efficient way for isolation and cultivation of human scalp dermal papilla cells.' J Dermatol Sci 37(1): 58-60. Lichti, U., W. C. Weinberg, L. Goodman, S. Ledbetter, et al. (1993). 'In vivo regulation of murine hair growth: insights from grafting defined cell populations onto nude mice.' J Invest Dermatol 101(1 Suppl): 124S-129S. Lin, R. Z. and H. Y. Chang (2008). 'Recent advances in three-dimensional multicellular spheroid culture for biomedical research.' Biotechnol J 3(9-10): 1172-1184. Lin, S. J., S. H. Jee, W. C. Hsaio, S. J. Lee, et al. (2005). 'Formation of melanocyte spheroids on the chitosan-coated surface.' Biomaterials 26(12): 1413-1422. Lin, S. J., S. H. Jee, W. C. Hsiao, H. S. Yu, et al. (2006). 'Enhanced cell survival of melanocyte spheroids in serum starvation condition.' Biomaterials 27(8): 1462-1469. Liu, Y. (1999). 'Understanding the biological activity of amyloid proteins in vitro: from inhibited cellular MTT reduction to altered cellular cholesterol homeostatis.' Prog Neuropsychopharmacol Biol Psychiatry 23(3): 377-395. Lu, H. F., K. N. Chua, P. C. Zhang, W. S. Lim, et al. (2005). 'Three-dimensional co-culture of rat hepatocyte spheroids and NIH/3T3 fibroblasts enhances hepatocyte functional maintenance.' Acta Biomater 1(4): 399-410. Martz, E., H. M. Phillips and M. S. Steinberg (1974). 'Contact inhibitions of overlapping and differential cell adhesion: a sufficient model for the control of certain cell culture morphologies.' J Cell Sci 16(2): 401-419. McElwee, K. J., S. Kissling, E. Wenzel, A. Huth, et al. (2003). 'Cultured peribulbar dermal sheath cells can induce hair follicle development and contribute to the dermal sheath and dermal papilla.' J Invest Dermatol 121(6): 1267-1275. Meidan, V. M. and E. Touitou (2001). 'Treatments for androgenetic alopecia and alopecia areata: current options and future prospects.' Drugs 61(1): 53-69. Messenger, A. G. (1984). 'The culture of dermal papilla cells from human hair follicles.' Br J Dermatol 110(6): 685-689. Mikkola, M. L. (2007). 'Genetic basis of skin appendage development.' Semin Cell Dev Biol 18(2): 225-236. Millar, S. E. (2002). 'Molecular mechanisms regulating hair follicle development.' J Invest Dermatol 118(2): 216-225. Mosmann, T. (1983). 'Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays.' J Immunol Methods 65(1-2): 55-63. Mou, C., B. Jackson, P. Schneider, P. A. Overbeek, et al. (2006). 'Generation of the primary hair follicle pattern.' Proc Natl Acad Sci U S A 103(24): 9075-9080. Okano, T., N. Yamada, H. Sakai and Y. Sakurai (1993). 'A novel recovery system for cultured cells using plasma-treated polystyrene dishes grafted with poly(N-isopropylacrylamide).' J Biomed Mater Res 27(10): 1243-1251. Oliver, R. F. (1966). 'Whisker growth after removal of the dermal papilla and lengths of follicle in the hooded rat.' J Embryol Exp Morphol 15(3): 331-347. Oliver, R. F. (1967). 'The experimental induction of whisker growth in the hooded rat by implantation of dermal papillae.' J Embryol Exp Morphol 18(1): 43-51. Orentreich, N. (1959). 'Autografts in alopecias and other selected dermatological conditions.' Ann N Y Acad Sci 83: 463-479. Osada, A., T. Iwabuchi, J. Kishimoto, T. S. Hamazaki, et al. (2007). 'Long-term culture of mouse vibrissal dermal papilla cells and de novo hair follicle induction.' Tissue Eng 13(5): 975-982. Otberg, N., H. Kang, A. A. Alzolibani and J. Shapiro (2008). 'Folliculitis decalvans.' Dermatol Ther 21(4): 238-244. Papadaki, M., T. Mahmood, P. Gupta, M. B. Claase, et al. (2001). 'The different behaviors of skeletal muscle cells and chondrocytes on PEGT/PBT block copolymers are related to the surface properties of the substrate.' J Biomed Mater Res 54(1): 47-58. Price, V. H. (1999). 'Treatment of hair loss.' N Engl J Med 341(13): 964-973. Qiao, J., A. Zawadzka, E. Philips, A. Turetsky, et al. (2009). 'Hair follicle neogenesis induced by cultured human scalp dermal papilla cells.' Regen Med 4(5): 667-676. Randall, V. A. (2008). 'Androgens and hair growth.' Dermatol Ther 21(5): 314-328. Randall, V. A., N. A. Hibberts, M. J. Thornton, K. Hamada, et al. (2000). 'The hair follicle: a paradoxical androgen target organ.' Horm Res 54(5-6): 243-250. Rendl, M., L. Lewis and E. Fuchs (2005). 'Molecular dissection of mesenchymal-epithelial interactions in the hair follicle.' PLoS Biol 3(11): e331. Reynolds, A. J. and C. A. Jahoda (1992). 'Cultured dermal papilla cells induce follicle formation and hair growth by transdifferentiation of an adult epidermis.' Development 115(2): 587-593. Rittmaster, R. S. (1994). 'Finasteride.' N Engl J Med 330(2): 120-125. Rivron, N. C., J. Rouwkema, R. Truckenmuller, M. Karperien, et al. (2009). 'Tissue assembly and organization: Developmental mechanisms in microfabricated tissues.' Biomaterials 30(28): 4851-4858. Ryan, P. L., R. A. Foty, J. Kohn and M. S. Steinberg (2001). 'Tissue spreading on implantable substrates is a competitive outcome of cell-cell vs. cell-substratum adhesivity.' Proc Natl Acad Sci U S A 98(8): 4323-4327. Sakai, Y., S. Yoshida, Y. Yoshiura, R. Mori, et al. (2010). 'Effect of microwell chip structure on cell microsphere production of various animal cells.' J Biosci Bioeng. Sasaki, D., T. Shimizu, S. Masuda, J. Kobayashi, et al. (2009). 'Mass preparation of size-controlled mouse embryonic stem cell aggregates and induction of cardiac differentiation by cell patterning method.' Biomaterials. Sawaya, M. E. and V. H. Price (1997). 'Different levels of 5alpha-reductase type I and II, aromatase, and androgen receptor in hair follicles of women and men with androgenetic alopecia.' J Invest Dermatol 109(3): 296-300. Schmidt-Ullrich, R. and R. Paus (2005). 'Molecular principles of hair follicle induction and morphogenesis.' Bioessays 27(3): 247-261. Schneider, M. R., R. Schmidt-Ullrich and R. Paus (2009). 'The hair follicle as a dynamic miniorgan.' Curr Biol 19(3): R132-142. Shen, M. and T. A. Horbett (2001). 'The effects of surface chemistry and adsorbed proteins on monocyte/macrophage adhesion to chemically modified polystyrene surfaces.' J Biomed Mater Res 57(3): 336-345. Soma, T., M. Tajima and J. Kishimoto (2005). 'Hair cycle-specific expression of versican in human hair follicles.' J Dermatol Sci 39(3): 147-154. Stenn, K. S., J. P. Sundberg and L. C. Sperling (1999). 'Hair follicle biology, the sebaceous gland, and scarring alopecias.' Arch Dermatol 135(8): 973-974. Tanahashi, K. and A. G. Mikos (2003). 'Effect of hydrophilicity and agmatine modification on degradation of poly(propylene fumarate-co-ethylene glycol) hydrogels.' J Biomed Mater Res A 67(4): 1148-1154. Teumer, J. and J. Cooley (2005). Follicular cell implantation: an emerging cell therapy for hair loss, THIEME MEDICAL PUBLISHERS. Tong, J. Z., P. De Lagausie, V. Furlan, T. Cresteil, et al. (1992). 'Long-term culture of adult rat hepatocyte spheroids.' Exp Cell Res 200(2): 326-332. Tosti, A. and B. Duque-Estrada (2009). 'Treatment strategies for alopecia.' Expert Opin Pharmacother 10(6): 1017-1026. Van Scott, E. J. and T. M. Ekel (1958). 'Geometric relationships between the matrix of the hair bulb and its dermal papilla in normal and alopecic scalp.' J Invest Dermatol 31(5): 281-287. von Bubnoff, D., E. Andres, F. Hentges, T. Bieber, et al. (2010). 'Natural killer cells in atopic and autoimmune diseases of the skin.' J Allergy Clin Immunol 125(1): 60-68. Wang, W., K. Itaka, S. Ohba, N. Nishiyama, et al. (2009). '3D spheroid culture system on micropatterned substrates for improved differentiation efficiency of multipotent mesenchymal stem cells.' Biomaterials 30(14): 2705-2715. Weiss, V. C., D. P. West and C. E. Mueller (1981). 'Topical minoxidil in alopecia areata.' J Am Acad Dermatol 5(2): 224-226. Whiting, D. A. (1998). 'Male pattern hair loss: current understanding.' Int J Dermatol 37(8): 561-566. Whiting, D. A. (2001). 'Advances in the treatment of male androgenetic alopecia: a brief review of finasteride studies.' Eur J Dermatol 11(4): 332-334. Whiting, D. A. (2003). 'Histopathologic features of alopecia areata: a new look.' Arch Dermatol 139(12): 1555-1559. Yang, M. J., C. H. Chen, P. J. Lin, C. H. Huang, et al. (2007). 'Novel method of forming human embryoid bodies in a polystyrene dish surface-coated with a temperature-responsive methylcellulose hydrogel.' Biomacromolecules 8(9): 2746-2752. Yen, C. M., C. C. Chan and S. J. Lin (2010). 'High-throughput reconstitution of epithelial-mesenchymal interaction in folliculoid microtissues by biomaterial-facilitated self-assembly of dissociated heterotypic adult cells.' Biomaterials 31(15): 4341-4352. Young, T. H. and C. H. Hung (2005). 'Behavior of embryonic rat cerebral cortical stem cells on the PVA and EVAL substrates.' Biomaterials 26(20): 4291-4299. Young, T. H., C. Y. Lee, H. C. Chiu, C. J. Hsu, et al. (2008). 'Self-assembly of dermal papilla cells into inductive spheroidal microtissues on poly(ethylene-co-vinyl alcohol) membranes for hair follicle regeneration.' Biomaterials 29(26): 3521-3530. Young, T. H., H. R. Tu, C. C. Chan, Y. C. Huang, et al. (2009). 'The enhancement of dermal papilla cell aggregation by extracellular matrix proteins through effects on cell-substratum adhesivity and cell motility.' Biomaterials 30(28): 5031-5040. Young, T. H., C. H. Yao, J. S. Sun, C. P. Lai, et al. (1998). 'The effect of morphology variety of EVAL membranes on the behavior of myoblasts in vitro.' Biomaterials 19(7-9): 717-724. Zappacosta, A. R. (1980). 'Reversal of baldness in patient receiving minoxidil for hypertension.' N Engl J Med 303(25): 1480-1481. Zheng, Y., X. Du, W. Wang, M. Boucher, et al. (2005). 'Organogenesis from dissociated cells: generation of mature cycling hair follicles from skin-derived cells.' J Invest Dermatol 124(5): 867-876. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/44655 | - |
dc.description.abstract | 禿髮,一種常見的疾病,其造成的心理創傷遠大於對生理方面的影響,所費不貲的植髮手術僅能重新分佈殘餘毛髮至禿髮部位且無法應用於嚴重落髮患者,因此具有誘導毛囊再生能力的毛囊真皮乳頭細胞成為目前最具潛力的解決方案。
真皮乳頭細胞需在聚集成多細胞球體狀態下,才能表現誘導表皮細胞分化成毛囊結構的能力,已有許多利用組織工程培養立體結構多細胞球的方法,但以特定生醫材料表面使細胞貼附並自我聚集的方法,遭遇如何輕易地收集多細胞球而不造成損壞之困擾。另一方面,懸浮培養方式之球體尺寸往往為一區間分佈而非固定尺寸。 根據細胞於親水性材料表面貼附不佳之特性,我們使用塗佈聚乙烯醇(poly(vinyl alcohol), PVA)的聚合酶鏈鎖反應(polymerase chain reaction, PCR)96孔盤中,由於狹小空間內的高碰撞頻率,我們成功地藉由操縱種植細胞的數量,在一天之內即可迅速獲得尺寸可控制的大鼠與人類真皮乳頭細胞球。 進一步分析顯示,大鼠與人類細胞球內細胞能維持80%以上存活率,均顯著高於懸滴培養方式,組織觀察球體內為緊密的細胞堆疊結構,並能維持真皮乳頭細胞與誘導能力有關的基因表現。我們也驗證真皮乳頭細胞球體再通過注射器之後能維持結構完整與細胞生長能力,於毛囊再生測試中,大鼠與人類真皮乳頭細胞球均能成功誘導毛囊再生,檢驗不同尺寸的真皮乳頭細胞球誘導能力,結果建議細胞球內需要達到一個最少的細胞數量值才能獲得較高的誘導效率。 我們成功地建立一個快速形成尺寸可控制之真皮乳頭細胞球的方法,並具有搭配自動化設備大量生產的潛力,我們的毛源性真皮乳頭細胞球大量生產系統未來也能應用於臨床上毛囊再生之研究。 | zh_TW |
dc.description.abstract | Hair loss is a common disorder that often causes more intense influence in psychological than physiological aspect. Expensive hair transplantation can only redistribute remaining hair to bald area and can no be applied to patients with extensive hair loss. Regarding this, hair follicle (HF) dermal papilla(DP) cells beome a promising solution due to their ability to induce HF regeneration.
DP cells exhibit the potential to guide the transdifferentiation of epidermal cells into follicular structure only when they are kept in multicellular aggregates. A number of tissue engineering approaches have been developed to produce three-dimensional multicellular spheroids. Methods depending on intercellular self-assembly of cells attached to biomaterials encounter difficulty in harmlessly harvesting multicellular spheroids with ease. On the other hand, suspension culture methods can result in variation in spheroid sizes. According to the poor adhesiveness of cells to hydrophilic surface, we culture DP cells in 96-well PCR plate coated with poly(vinyl alcohol)(PVA). Due to high collision frequency in limited space, we can obtain size-controllable human or rat DP spheres rapidly within 24 hours in culture by varying the numbers of cells seeded. Further analysis reveals that more than 80% of cells within the spheres are viable and this viability is higher than that in spheres obtained by hanging drop method. DP spheres have a compact structure in histology and preserve DP signature gene expression profile that is related to the HF induction ability. We also demonstrate that the structure and cellular viability of DP spheres remain intact after they are injected through a cell delivery injection apparatus. Functionally, both human and rat DP spheres can successfully induce the HF regeneration in HF regeneration assay. The HF induction ability is varied when DP spheres of various sizes are tested and our results suggest that a minimum cell number is required within each DP sphere to obtain a higher HF induction efficiency. We successfully set up an easy method for fast generation of size-controllable DP cell spheres and this method is of scalable potential with automatic equipment. Our system of mass preparation of trichogenic DP spheres can also be applied to clinical investigation for HF regeneration in the future. | en |
dc.description.provenance | Made available in DSpace on 2021-06-15T03:52:23Z (GMT). No. of bitstreams: 1 ntu-99-R97548033-1.pdf: 6475090 bytes, checksum: d60222020875ce8dc21d813659a6331a (MD5) Previous issue date: 2010 | en |
dc.description.tableofcontents | Abstract I
摘 要 III 目 錄 IV 圖目錄 VII 第一章、序論 1 1.1. 毛囊結構 1 1.2. 毛囊發育與週期 4 1.3. 落髮 6 1.4. 現行治療方式 9 1.5. 組織工程發展毛囊再生之研究 10 1.6. 細胞聚集 12 1.7. 生醫高分子材料於組織工程之應用 13 第二章、研究目標與實驗設計 14 2.1. 研究目標 14 2.2. 實驗設計 17 第三章、材料與方法 20 3.1. 人類與大鼠真皮乳頭細胞培養 20 3.2. 人類包皮表皮細胞培養 21 3.3. 高分子材料製備 21 3.4. 接觸角測量 22 3.5. 細胞貼附特性 23 3.6. 細胞密度與材料 23 3.7. 懸滴培養 23 3.8. 均勻細胞球體培養 24 3.9. 細胞球體半徑與形狀因子計算 24 3.10. 細胞球體細胞總數與存活率 24 3.11. 比色法檢驗活性表現 MTT assay 25 3.12. RNA 萃取 26 3.13. 反轉錄 26 3.14. 聚合酶鏈鎖反應 27 3.15. 細胞球體結構觀察 28 3.16. 鹼性磷酸酶染色 alkaline phosphatase staining 28 3.17. 免疫染色 29 3.18. 注射後完整性及生長能力測試 29 3.19. 動物移植實驗 30 3.20. 誘導新生毛囊效率計算 31 第四章、實驗結果 32 4.1. 不同親疏水性材料表面之水滴接觸角差異 32 4.2. 大鼠與人類真皮乳頭細胞貼附 35 4.3. 大鼠與人類真皮乳頭細胞於不同密度及材料表面之生長型態 41 4.4. 懸滴培養大鼠與人類真皮乳頭細胞於體外模擬真皮乳頭組織 49 4.5. 於塗佈PVA表面之PCR tube培養人類與大鼠真皮乳頭細胞 53 4.6. 種植細胞數與成球直徑之數學關係式 59 4.7. 細胞球體內存活比例 65 4.8. 細胞球體內活細胞活性 75 4.9. 細胞球體構造 83 4.10. 細胞球體基因表現 84 4.11. 細胞球體alkaline phosphatase酵素檢驗 86 4.12. 細胞球體切片免疫染色 87 4.13. 細胞球體注射後完整性與生長能力 89 4.14. 真皮乳頭細胞球誘導毛囊再生 95 4.15. 誘導毛囊再生效率計算 98 4.16. 新生毛囊結構 100 第五章、討論 102 第六章、結論 106 第七章、參考文獻 107 | |
dc.language.iso | zh-TW | |
dc.title | 在聚乙烯醇表面大量製造大小可調控的可注入性真皮乳頭微組織以應用於毛囊再生 | zh_TW |
dc.title | Mass production of injectable dermal papilla spheroids of controllable size on poly(vinyl alcohol) surface for hair follicle regeneration | en |
dc.type | Thesis | |
dc.date.schoolyear | 98-2 | |
dc.description.degree | 碩士 | |
dc.contributor.coadvisor | 林頌然 | |
dc.contributor.oralexamcommittee | 吳君泰,林致廷 | |
dc.subject.keyword | 毛囊再生,三度空間立體培養,真皮乳頭組織,聚乙烯醇,尺寸控制, | zh_TW |
dc.subject.keyword | hair follicle regeneration,three dimension culture,dermal papilla,poly(vinyl alcohol),size control, | en |
dc.relation.page | 116 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2010-07-08 | |
dc.contributor.author-college | 工學院 | zh_TW |
dc.contributor.author-dept | 醫學工程學研究所 | zh_TW |
顯示於系所單位: | 醫學工程學研究所 |
文件中的檔案:
檔案 | 大小 | 格式 | |
---|---|---|---|
ntu-99-1.pdf 目前未授權公開取用 | 6.32 MB | Adobe PDF |
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。