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http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/4451
標題: | 重組鞘蛋白抑制粉蝨傳播番茄黃化捲葉泰國病毒 Recombinant Coat Protein Suppress Whitefly Transmission of Tomato yellow leaf curl Thailand virus |
作者: | Chien-Hao Tseng 曾建豪 |
指導教授: | 蔡志偉 |
關鍵字: | begomoviruses,菸草粉蝨,重組病毒蛋白,病媒傳播,免疫螢光分析, begomoviruses,Bemisia tabaci,recombinant viral protein,vector transmission,immunofluorescence assay, |
出版年 : | 2015 |
學位: | 碩士 |
摘要: | 粉蝨不僅藉由取食造成植株的危害,更做為媒介昆蟲傳播植物病毒。菸草粉蝨傳播的 begomoviruses 威脅世界上許多作物的栽培。番茄黃化捲葉泰國病毒 (Tomato yellow leaf curl Thailand virus, TYLCTHV) 及番茄捲葉台灣病毒 (Tomato leaf curl Taiwan virus, ToLCTWV) 是臺灣番茄田間最常見的兩種病毒。鞘蛋白 (coat protein, CP) 是 begomoviruses 唯一的結構蛋白,CP 被推測與菸草粉蝨中腸推定的受體 (receptor) 有交互作用。In vivo binding assay 結果顯示 recombinant TYLCTHV CP與菸草粉蝨 (Bemisia tabaci) 的中腸細胞有專一性的結合。本研究的目的是探討菸草粉蝨獲得病毒前先餵食 recombinant TYLCTHV CP 是否會競爭粉蝨中腸推定的受體,進而降低其獲得 TYLCTHV 病毒量與粉蝨傳播TYLCTHV的能力。實驗結果顯示先餵食 recombinant TYLCTHV CP 會降低 TYLCTHV 在粉蝨中腸細胞內的累積程度,但是卻沒有降低粉蝨體內的 TYLCTHV 病毒量。此外,先餵食 recombinant TYLCTHV CP會抑制粉蝨傳播TYLCTHV。本研究也利用相似的實驗來探討先餵食 recombinant TYLCTHV CP (homologous to ToLCTWV CP) 是否也會降低粉蝨中腸細胞內 ToLCTWV 的累積程度與粉蝨體內的病毒量,以及抑制粉蝨傳播 ToLCTWV。實驗結果指出先餵食 recombinant TYLCTHV CP不會降低 ToLCTWV 在菸草粉蝨中腸細胞內的累積程度與粉蝨體內的 ToLCTWV 病毒量。此外,先餵食 recombinant TYLCTHV CP也不會抑制菸草粉蝨傳播ToLCTWV。本研究證實 TYLCTHV CP 做為 attachment protein的功能並且能抑制菸草粉蝨傳播 TYLCTHV。此外,餵食 recombinant TYLCTHV CP 無法有效降低菸草粉蝨傳播 ToLCTWV 的結果也顯示 TYLCTHV CP與菸草粉蝨中腸的 receptor 具有專一性。了解 CP 與菸草粉蝨中腸的交互作用不僅可以揭開菸草粉蝨傳播 begomoviruses 的機制,日後進一步的研究更能幫助我們發展對抗病害的防治策略。 Whiteflies not only damage plants through feeding but also serve as vectors transmitting plant viruses. Whitefly-transmitted begomoviruses threaten a wide range of crops worldwide. Tomato yellow leaf curl Thailand virus (TYLCTHV) and Tomato leaf curl Taiwan virus (ToLCTWV) are two most prevalent viruses in tomato fields in Taiwan. Coat protein (CP), the only structural protein of begomoviruses, is hypothesized to interact with a putative receptor in the midgut of Bemisia tabaci. In vivo binding assay demonstrated recombinant TYLCTHV CP specifically bound to the midgut cells of B. tabaci. The objective of this study was to determine if preacquisition of TYLCTHV CP competes the putative receptor in the midgut of B. tabaci hence suppresses the acquisition and vector transmission of TYLCTHV. Preacquisition of recombinant TYLCTHV CP decreased the accumulation level of TYLCTHV in the midgut cells of B. tabaci but did not reduce the acquisition of TYLCTHV in B. tabaci. Further, preacquisition of recombinant TYLCTHV CP suppressed the whitefly transmission of TYLCTHV. Similar experiments were conducted to investigate if preacquisition of TYLCTHV CP (homologous to ToLCTWV CP) would reduce virus accumulation and acquisition in B. tabaci, and the whitefly transmission of ToLCTWV. The results demonstrated that preacquisition of recombinant TYLCTHV CP did not influence the accumulation and acquisition of ToLCTWV in B. tabaci and did neither reduce the whitefly transmission of ToLCTWV. In conclusion, TYLCTHV CP functioned as an attachment protein and preacquisition of TYLCTHV CP reduced the whitefly transmission of TYLCTHV. The failure that using TYLCTHV CP to inhibit the whitefly transmission of ToLCTWV suggested that the interaction between TYLCTHV CP and the putative receptor in the midgut of B. tabaci is specific. Understanding the CP-midgut interaction would unveil the mechanism of whitefly transmission of begomoviruses and warrant further study to develop strategies for disease control. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/4451 |
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顯示於系所單位: | 昆蟲學系 |
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