請用此 Handle URI 來引用此文件：
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/44428完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 黃鵬鵬 | |
| dc.contributor.author | Feng-Chun Ting | en |
| dc.contributor.author | 丁楓峻 | zh_TW |
| dc.date.accessioned | 2021-06-15T02:57:02Z | - |
| dc.date.available | 2009-08-20 | |
| dc.date.copyright | 2009-08-20 | |
| dc.date.issued | 2009 | |
| dc.date.submitted | 2009-08-03 | |
| dc.identifier.citation | Abbink, W., Hang, X. M., Guerreiro, P. M., Spanings, F. A., Ross, H. A., Canario, A. V., Flik, G., 2007. Parathyroid hormone-related protein and calcium regulation in vitamin D-deficient sea bream (Sparus auratus). J Endocrinol. 193, 473-80.
Anne-Gaëlle Lafont*, Bo-Kai Liao, Yung-Che Tseng and Pung-Pung Hwang, 2009. Characterization and expression of the calcitonin gene and its receptors in zebrafish (Danio rerio): involvement of CT in the calcium regulation. In preparation. Bevelander, G., Pinto, E., Canario, A., Spanings, T., Flik, G., 2008. CYP27A1 expression in gilthead sea bream (Sparus auratus, L.): effects of calcitriol and parathyroid hormone-related protein. Journal of Endocrinology. 196, 625. Bijvelds, M. J., Velden, J. A., Kolar, Z. I., Flik, G., 1998. Magnesium transport in freshwater teleosts. J Exp Biol. 201 (Pt 13), 1981-90. Chang, I. C., Lee, T. H., Yang, C. H., Wei, Y. Y., Chou, F. I., Hwang, P. P., 2001. Morphology and function of gill mitochondria-rich cells in fish acclimated to different environments. Physiol Biochem Zool. 74, 111-9. Chen, Y. Y., Lu, F. I., Hwang, P. P., 2003. Comparisons of calcium regulation in fish larvae. J Exp Zoolog A Comp Exp Biol. 295, 127-35. Chou, M. Y., Yang, C. H., Lu, F. I., Lin, H. C., Hwang, P. P., 2002. Modulation of calcium balance in tilapia larvae (Oreochromis mossambicus) acclimated to low-calcium environments. J Comp Physiol [B]. 172, 109-14. Ciesielski, F., Rochel, N., Mitschler, A., Kouzmenko, A., Moras, D., 2004. Structural investigation of the ligand binding domain of the zebrafish VDR in complexes with 1alpha,25(OH)2D3 and Gemini: purification, crystallization and preliminary X-ray diffraction analysis. J Steroid Biochem Mol Biol. 89-90, 55-9. Ciesielski, F., Rochel, N., Moras, D., 2007. Adaptability of the Vitamin D nuclear receptor to the synthetic ligand Gemini: remodelling the LBP with one side chain rotation. J Steroid Biochem Mol Biol. 103, 235-42. Craig, T. A., Sommer, S., Sussman, C. R., Grande, J. P., Kumar, R., 2008. Expression and regulation of the vitamin D receptor in the zebrafish, Danio rerio. J Bone Miner Res. 23, 1486-96. Cui, J., Kaandorp, J. A., Ositelu, O. O., Beaudry, V., Knight, A., Nanfack, Y. F., Cunningham, K. W., 2009. Simulating calcium influx and free calcium concentrations in yeast. Cell Calcium. 45, 123-132. DeLuca, H. F., 2004. Overview of general physiologic features and functions of vitamin D. Am J Clin Nutr. 80, 1689S-96S. Dusso, A. S., Brown, A. J., Slatopolsky, E., 2005. Vitamin D. Am J Physiol Renal Physiol. 289, F8-28. Evans, D. H., Piermarini, P. M., Choe, K. P., 2005. The multifunctional fish gill: dominant site of gas exchange, osmoregulation, acid-base regulation, and excretion of nitrogenous waste. Physiol Rev. 85, 97-177. Fleming, A., Sato, M., Goldsmith, P., 2005. High-throughput in vivo screening for bone anabolic compounds with zebrafish. J Biomol Screen. 10, 823-31. Flik, G., Klaren, P., Schoenmakers, T., Bijvelds, M., Verbost, P., 1996. Cellular calcium transport in fish: unique and universal mechanisms. Flik, G., Verbost, P., Wendelaar Bonga, S., 1995. Calcium transport processes in fishes. Fish physiology. 14, 317–342. Gogvadze, V., Orrenius, S., Zhivotovsky, B., 2009. Mitochondria as targets for chemotherapy. Apoptosis. 14, 624-40. Hoenderop, J. G., Hartog, A., Stuiver, M., Doucet, A., Willems, P. H., Bindels, R. J., 2000. Localization of the epithelial Ca2+ channel in rabbit kidney and intestine. J Am Soc Nephrol. 11, 1171-8. Hoenderop, J. G., Nilius, B., Bindels, R. J., 2005. Calcium absorption across epithelia. Physiol Rev. 85, 373-422. Hogstrand, C., Verbost, P. M., Bonga, S. E., Wood, C. M., 1996. Mechanisms of zinc uptake in gills of freshwater rainbow trout: interplay with calcium transport. Am J Physiol. 270, R1141-7. Howarth, D. L., Law, S. H., Barnes, B., Hall, J. M., Hinton, D. E., Moore, L., Maglich, J. M., Moore, J. T., Kullman, S. W., 2008. Paralogous vitamin D receptors in teleosts: transition of nuclear receptor function. Endocrinology. 149, 2411-22. Hwang, P., Lee, T., 2007a. New insights into fish ion regulation and mitochondrion-rich cells. Comparative Biochemistry and Physiology, Part A. 148, 479-497. Hwang, P., Tsai, Y., Tung, Y., 1994. Calcium balance in embryos and larvae of the freshwater-adapted teleost, Oreochromis mossambicus. Fish Physiology and Biochemistry. 13, 325-333. Hwang, P. P., Lee, T. H., 2007b. New insights into fish ion regulation and mitochondrion-rich cells. Comp Biochem Physiol A Mol Integr Physiol. 148, 479-97. Jurutka, P. W., Whitfield, G. K., Hsieh, J. C., Thompson, P. D., Haussler, C. A., Haussler, M. R., 2001. Molecular nature of the vitamin D receptor and its role in regulation of gene expression. Rev Endocr Metab Disord. 2, 203-16. Krasowski, M. D., Yasuda, K., Hagey, L. R., Schuetz, E. G., 2005. Evolutionary selection across the nuclear hormone receptor superfamily with a focus on the NR1I subfamily (vitamin D, pregnane X, and constitutive androstane receptors). Nucl Recept. 3, 2. Liao, B. K., Deng, A. N., Chen, S. C., Chou, M. Y., Hwang, P. P., 2007. Expression and water calcium dependence of calcium transporter isoforms in zebrafish gill mitochondrion-rich cells. BMC Genomics. 8, 354. Lock, E. J., Ornsrud, R., Aksnes, L., Spanings, F. A., Waagbo, R., Flik, G., 2007. The vitamin D receptor and its ligand 1alpha,25-dihydroxyvitamin D3 in Atlantic salmon (Salmo salar). J Endocrinol. 193, 459-71. Maglich, J. M., Caravella, J. A., Lambert, M. H., Willson, T. M., Moore, J. T., Ramamurthy, L., 2003. The first completed genome sequence from a teleost fish (Fugu rubripes) adds significant diversity to the nuclear receptor superfamily. Nucleic Acids Res. 31, 4051-8. Marshall, W. S., 2002. Na+, Cl-, Ca2+ and Zn2+ transport by fish gills: retrospective review and prospective synthesis. J Exp Zool. 293, 264-83. Marshall, W. S., Bryson, S. E., 1998. Transport mechanisms of seawater teleost chloride cells: an inclusive model of a multifunctional cell. Comp Biochem Physiol A Mol Integr Physiol. 119, 97-106. Marshall, W. S., Bryson, S. E., Wood, C. M., 1992. Calcium transport by isolated skin of rainbow trout. J Exp Biol. 166, 297-316. McCormick, S. D., Hasegawa, S., Hirano, T., 1992. Calcium uptake in the skin of a freshwater teleost. Proc Natl Acad Sci U S A. 89, 3635-8. Pan, T. C., Liao, B. K., Huang, C. J., Lin, L. Y., Hwang, P. P., 2005. Epithelial Ca2+ channel expression and Ca2+ uptake in developing zebrafish. Am J Physiol Regul Integr Comp Physiol. 289, R1202-11. Perry, S. V., 1985. Properties of the muscle proteins--a comparative approach. J Exp Biol. 115, 31-42. Pisam, M., Le Moal, C., Auperin, B., Prunet, P., Rambourg, A., 1995. Apical structures of 'mitochondria-rich' alpha and beta cells in euryhaline fish gill: their behaviour in various living conditions. Anat Rec. 241, 13-24. Reschly, E. J., Bainy, A. C., Mattos, J. J., Hagey, L. R., Bahary, N., Mada, S. R., Ou, J., Venkataramanan, R., Krasowski, M. D., 2007. Functional evolution of the vitamin D and pregnane X receptors. BMC Evol Biol. 7, 222. Reschly, E. J., Krasowski, M. D., 2006. Evolution and function of the NR1I nuclear hormone receptor subfamily (VDR, PXR, and CAR) with respect to metabolism of xenobiotics and endogenous compounds. Curr Drug Metab. 7, 349-65. Shaffer, P., Gewirth, D., 2002. Structural basis of VDR-DNA interactions on direct repeat response elements. EMBO J. 21, 2242 - 52. Suzuki, T., Suzuki, N., Srivastava, A. S., Kurokawa, T., 2000. Identification of cDNAs encoding two subtypes of vitamin D receptor in flounder, Paralichthys olivaceus. Biochem Biophys Res Commun. 270, 40-5. Thisse, B., Heyer, V., Lux, A., Alunni, V., Degrave, A., Seiliez, I., Kirchner, J., Parkhill, J. P., Thisse, C., 2004. Spatial and temporal expression of the zebrafish genome by large-scale in situ hybridization screening. Methods Cell Biol. 77, 505-19. Tordoff, M., 2001. Calcium: taste, intake, and appetite. Physiological reviews. 81, 1567-1597. Tseng, D. Y., Chou, M. Y., Tseng, Y. C., Hsiao, C. D., Huang, C. J., Kaneko, T., Hwang, P. P., 2009. Effects of stanniocalcin 1 on calcium uptake in zebrafish (Danio rerio) embryo. Am J Physiol Regul Integr Comp Physiol. 296, R549-57. Verbost, P., Flik, G., Fenwick, J., Greco, A., Pang, P., Wendelaar Bonga, S., 1993. Branchial calcium uptake: possible mechanisms of control by stanniocalcin. Fish Physiology and Biochemistry. 11, 205-215. Wagner, G. F., Dimattia, G. E., Davie, J. R., Copp, D. H., Friesen, H. G., 1992. Molecular cloning and cDNA sequence analysis of coho salmon stanniocalcin. Mol Cell Endocrinol. 90, 7-15. Walker, W., Liem, K., 1994. Functional anatomy of the vertebrates: an evolutionary perspective. Saunders College. Whitfield, G. K., Dang, H. T., Schluter, S. F., Bernstein, R. M., Bunag, T., Manzon, L. A., Hsieh, G., Dominguez, C. E., Youson, J. H., Haussler, M. R., Marchalonis, J. J., 2003. Cloning of a functional vitamin D receptor from the lamprey (Petromyzon marinus), an ancient vertebrate lacking a calcified skeleton and teeth. Endocrinology. 144, 2704-16. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/44428 | - |
| dc.description.abstract | 鈣離子參與許多重要的生理反應，因此動物如何調控鈣離子恆定是相當重要的議題。淡水環境中的鈣離子濃度變化劇烈，當淡水魚成魚面對環境鈣離子變化時，位於鰓上皮組織的富含粒線體細胞(mitochondria-rich cell, MR cell)上的離子通道或運輸蛋白會受到調控並改變表現，進而維持體內的離子變動不會過於激烈。然而在淡水魚的胚胎發育時期，鰓並未發育完全，離子調控主要透過一些位在表皮上的富含粒線體細胞。對於內分泌調控魚類鈣離子吸收機制的研究目前非常少，已知在哺乳動物的實驗模式中，vitamin D可以調控腸道和腎臟的表皮鈣離子通道 (Epithelium calcium channel, ECaC)表現量，但在魚類仍不清楚，是否在原始的魚類中就已存在維他命D調控鈣離子吸收機制值得進一步探討。
本實驗探討維他命D對斑馬魚鈣離子吸收機制的影響，結果發現維他命D受器基因(vitamin D receptor, VDR)在胚胎受精後三小時內就開始表現；另一方面，並發現VDR基因廣泛分佈在成魚的各組織中。將斑馬魚胚胎浸泡維他命D以後分析魚體總鈣含量、鈣離子流進胚胎體內的速率及與鈣離子相關的離子通道或運輸蛋白的基因表現量，結果發現皆有上昇的趨勢。利用Morpholino默化 (knockdown) 斑馬魚維他命D-a型受器(zVDRa)的表現，發現到胚胎體內的鈣離子含量、鈣離子流進體內的速率和 zECaC mRNA 的表現量，都有顯著的下降，但是卻不影響 zNCX1b和 zPMCA2的表現量。藉由上述結果推論斑馬魚胚胎在適應鈣環境改變過程中，維他命D是扮演調控鈣離子吸收機制的重要因素之一 。 | zh_TW |
| dc.description.abstract | How animals maintain Ca2+ balance is an important issue because Ca2+ serves a variety of physiological roles. Ca2+ concentration usually fluctuates in freshwater environment. Facing Ca2+ changes, aquatic vertebrates must adjust Ca2+ absorption ability by regulating the expression of apical epithelial calcium channel (ECaC) on skin epithelial mitochondria-rich cells (MR-cells) to compensate intracellular Ca2+ level. During embryo stage, however, gills of freshwater teleosts are not well developed. At this stage, MR-cells on skin epithelium are main roles for ion regulation. To date, there are fewer studies on how endocrines regulate Ca2+absorption in teleosts, while a number of studies have shown that vitamin D affects the expression of ECaC in intestine and kidney in mammals. To see if this mechanism proposed in mammals has already existed in fish, which are more primitive than mammals in the aspect of evolution, is valuable for further research.
The present study focused on effects of vitamin D on Ca2+ absorption in zebrafish. The mRNA expression of zebrafish vitamin D receptor-a (zVDRa) was detected as early as 3 hours postfertilization (hpf) and ubiquitously expressed in all tissues of zebrafish. Moreover, Ca2+ content, Ca2+ influx, and mRNA expression of zECaC were all increased, following vitamin D treatment. Finally, we found that Ca2+ content, Ca2+influx and the mRNA expression of zECaC decreased after zVDRa morpholino injection, but the expression of zebrafish Na+-Ca2+ exchanger 1b (zNCX1b) and zebrafish plasma membrane Ca2+-ATPase 2 (zPMCA2) were not affected. This study suggests that during the adaptation to fluctuating Ca2+ environment, vitamin D is one of the important factors regulating Ca2+ absorption in zebrafish embryos. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-15T02:57:02Z (GMT). No. of bitstreams: 1 ntu-98-R95b45005-1.pdf: 1226905 bytes, checksum: 7ea98cd1a5a0e372755dad1e7063b84d (MD5) Previous issue date: 2009 | en |
| dc.description.tableofcontents | Acknowledgement 1
摘要 2 Abstract 3 Introduction 5 The importance of Ca2+ to vertebrates 5 Ca2+channels and transporters for Ca2+ absorption 6 Endocrine control of Ca2+ regulation in teleost fish 7 Vitamin D and its receptor in fish 8 Purpose of this study 11 Materials and Methods 12 Experimental animals 12 Test chemicals and concentrations 12 Prolonged zebrafish embryo test (96 h) 12 Measurement of the whole-body Ca2+ content 13 Measurement of whole-body Ca2+ influx 14 RNA extraction 15 Reverse transcriptase PCR (RT-PCR) analysis 15 Quantitative real-time RT-PCR 16 Microinjection of antisense morpholino oligonucleotides (MOs) 16 Statistical analysis 17 Structural Analysis 17 Results 18 Expression patterns of zVDRa in various tissues of zebrafish 18 Effects of environmental Ca2+ levels on zVDRa and CYP27A1-like mRNA expressions 18 Effects of 3-day exposure to vitamin D on growth rate, Ca2+ content, Ca2+ influx and Ca2+ absorption related genes mRNA expressions in zebrafish embryos 19 Effects of the zVDRa morpholino on Ca2+ content, Ca2+influx and Ca2+ absorption related genes mRNA expressions in zebrafish embryos 19 Structural characterization of zECaC 20 Discussion 21 Perspectives and Significance 24 References 26 Tables and Figures 30 | |
| dc.language.iso | en | |
| dc.subject | 受器 | zh_TW |
| dc.subject | 鈣 | zh_TW |
| dc.subject | 維他命D | zh_TW |
| dc.subject | 硬骨魚 | zh_TW |
| dc.subject | 離子調節 | zh_TW |
| dc.subject | teleosts | en |
| dc.subject | receptors | en |
| dc.subject | ionoregulation | en |
| dc.subject | Calcium | en |
| dc.subject | vitamin D | en |
| dc.title | 維他命D對斑馬魚胚胎鈣離子吸收之影響 | zh_TW |
| dc.title | Effects of Vitamin D on Calcium Absorption in Zebrafish Embryos (Danio rerio) | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 97-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 張清風,林豊益,李宗翰 | |
| dc.subject.keyword | 鈣,維他命D,硬骨魚,離子調節,受器, | zh_TW |
| dc.subject.keyword | Calcium,vitamin D,teleosts,ionoregulation,receptors, | en |
| dc.relation.page | 43 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2009-08-03 | |
| dc.contributor.author-college | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 漁業科學研究所 | zh_TW |
| 顯示於系所單位： | 漁業科學研究所 | |
文件中的檔案：
| 檔案 | 大小 | 格式 | |
|---|---|---|---|
| ntu-98-1.pdf 未授權公開取用 | 1.2 MB | Adobe PDF |
系統中的文件，除了特別指名其著作權條款之外，均受到著作權保護，並且保留所有的權利。