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| ???org.dspace.app.webui.jsptag.ItemTag.dcfield??? | Value | Language |
|---|---|---|
| dc.contributor.advisor | 李士傑老師(Shyh-Jye Lee) | |
| dc.contributor.author | Chun-Wei Lin | en |
| dc.contributor.author | 林浚緯 | zh_TW |
| dc.date.accessioned | 2021-06-15T02:55:23Z | - |
| dc.date.available | 2009-08-04 | |
| dc.date.copyright | 2009-08-04 | |
| dc.date.issued | 2009 | |
| dc.date.submitted | 2009-08-03 | |
| dc.identifier.citation | Andrianantoandro, E., Pollard, T. D., 2006. Mechanism of actin filament turnover by severing and nucleation at different concentrations of ADF/cofilin. Mol Cell. 24, 13-23.
Carlier, M. F., et al., 1999. Control of actin dynamics in cell motility. Role of ADF/cofilin. J Biol Chem. 274, 33827-30. Dedova, I. V., et al., 2004. Two opposite effects of cofilin on the thermal unfolding of F-actin: a differential scanning calorimetric study. Biophys Chem. 110, 119-28. Foletta, V. C., et al., 2004. LIM kinase 1, a key regulator of actin dynamics, is widely expressed in embryonic and adult tissues. Exp Cell Res. 294, 392-405. Huang, T. Y., et al., 2006. Cofilin phosphatases and regulation of actin dynamics. Curr Opin Cell Biol. 18, 26-31. Huber, F., et al., 2008. Growing actin networks form lamellipodium and lamellum by self-assembly. Biophys J. 95, 5508-23. Kimmel, C. B., et al., 1995. Stages of embryonic development of the zebrafish. Dev Dyn. 203, 253-310. Koppen, M., et al., 2006. Coordinated cell-shape changes control epithelial movement in zebrafish and Drosophila. Development. 133, 2671-81. Lachnit, M., et al., 2008. Alterations of the cytoskeleton in all three embryonic lineages contribute to the epiboly defect of Pou5f1/Oct4 deficient MZspg zebrafish embryos. Dev Biol. 315, 1-17. Lappalainen, P., Drubin, D. G., 1997. Cofilin promotes rapid actin filament turnover in vivo. Nature. 388, 78-82. Lappalainen, P., et al., 1998. The ADF homology (ADF-H) domain: a highly exploited actin-binding module. Mol Biol Cell. 9, 1951-9. McCullough, B. R., et al., 2008. Cofilin increases the bending flexibility of actin filaments: implications for severing and cell mechanics. J Mol Biol. 381, 550-8. McGough, A., et al., 1997. Cofilin changes the twist of F-actin: implications for actin filament dynamics and cellular function. J Cell Biol. 138, 771-81. Mogilner, A., Oster, G., 1996. Cell motility driven by actin polymerization. Biophys J. 71, 3030-45. Mouneimne, G., et al., 2006. Spatial and temporal control of cofilin activity is required for directional sensing during chemotaxis. Curr Biol. 16, 2193-205. Mouneimne, G., et al., 2004. Phospholipase C and cofilin are required for carcinoma cell directionality in response to EGF stimulation. J Cell Biol. 166, 697-708. Niwa, R., et al., 2002. Control of actin reorganization by Slingshot, a family of phosphatases that dephosphorylate ADF/cofilin. Cell. 108, 233-46. Ohashi, K., et al., 2000. A Drosophila homolog of LIM-kinase phosphorylates cofilin and induces actin cytoskeletal reorganization. Biochem Biophys Res Commun. 276, 1178-85. Paavilainen, V. O., et al., 2007. Structural basis and evolutionary origin of actin filament capping by twinfilin. Proc Natl Acad Sci U S A. 104, 3113-8. Pavlov, D., et al., 2007. Actin filament severing by cofilin. J Mol Biol. 365, 1350-8. Pollard, T. D., Borisy, G. G., 2003. Cellular motility driven by assembly and disassembly of actin filaments. Cell. 112, 453-65. Shimizu, T., et al., 2005. E-cadherin is required for gastrulation cell movements in zebrafish. Mech Dev. 122, 747-63. Sumi, T., et al., 1999. Cofilin phosphorylation and actin cytoskeletal dynamics regulated by rho- and Cdc42-activated LIM-kinase 2. J Cell Biol. 147, 1519-32. Theriot, J. A., Mitchison, T. J., 1991. Actin microfilament dynamics in locomoting cells. Nature. 352, 126-31. Thisse, C., et al., 1994. Goosecoid expression in neurectoderm and mesendoderm is disrupted in zebrafish cyclops gastrulas. Dev Biol. 164, 420-9. Thisse, C., et al., 1993. Structure of the zebrafish snail1 gene and its expression in wild-type, spadetail and no tail mutant embryos. Development. 119, 1203-15. Trinkaus, J. P., 1980. Formation of protrusions of the cell surface during tissue cell movement. Prog Clin Biol Res. 41, 887-906. Trinkaus, J. P., 1992. The midblastula transition, the YSL transition and the onset of gastrulation in Fundulus. Dev Suppl. 75-80. Van Troys, M., et al., 2008. Ins and outs of ADF/cofilin activity and regulation. Eur J Cell Biol. 87, 649-67. Warga, R. M., Kimmel, C. B., 1990. Cell movements during epiboly and gastrulation in zebrafish. Development. 108, 569-80. Welch, M. D., et al., 1997. Actin dynamics in vivo. Curr Opin Cell Biol. 9, 54-61. Zalik, S. E., et al., 1999. Cell adhesion and the actin cytoskeleton of the enveloping layer in the zebrafish embryo during epiboly. Biochem Cell Biol. 77, 527-42. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/44400 | - |
| dc.description.abstract | 肌動蛋白去聚合蛋白(cofilin)為肌動蛋白去聚合因子(ADF/cofilin)的一種，能夠對肌動蛋白actin的聚合/去聚合進行調控，並且控制移動細胞中的偽足延伸。
為了探究cofilin在原腸期細胞遷移的功能，我們已從斑馬魚染色體組中分離出cofilin 1 (cfl1)；並且透過反轉錄酶-聚合酶鏈鎖反應(RT-PCR)與全標本原位雜交(WISH)得知，cfl1於早期胚胎發育中廣泛的表現在胚體的各部位。我們也已呈現過，針對cfl1的抑制，將造成包括在外包(epiboly)和集中與延伸(convergence and extension)時的細胞遷移產生缺陷。再者，我們透過細胞移植(cell transplantation)發現cfl1 MO(morpholino oligonucleotide)所產生的影響是細胞自主性的(cell- autonomous)。另外，進行內捲(involution)的偽足數量也會受到抑制cfl1的影響而有顯著地下降。我們更進一步透過穿透式電子顯微鏡與共軛交螢光顯微鏡的觀察，發現進行外包的包覆層(enveloping layer, EVL)與深細胞層(deep cell layer, DEL)在cfl1被抑制後，兩細胞層間的細胞附著變得鬆散。上述的實驗顯示，cfl1在斑馬原腸胚後期的細胞遷移過程中，扮演重要的角色。 | zh_TW |
| dc.description.abstract | Cofilins are members of actin-depolymerizing factor (ADF)/cofilin protein family that regulate actin treadmilling and the extension of pseudopods in motile cells. To study cofilin functions in gastrulation cell migration, we have isolated cofilin 1 (cfl1) from zebrafish genome, and its expression and functions have been examined. By RT-PCR and whole-mount in situ hybridization, cfl1 has been found to be ubiquitously expressed during early embryogenesis. We have also demonstrated that knockdown of cfl1 interfered cellular migration during epiboly, convergence and extension. Furthermore, the effects of cfl1 MO was found to be cell autonomous by transplantation experiments. In addition, pseudopods in the involuting cells were significantly reduced in the cfl1 morphants. The effects of cfl1 MO was further evident by the loose cell adhesions between enveloping layer (EVL) and deep cell layer (DEL) through transmission electronic microscopy and confocal microscopy. These results suggest that cfl1 participates in epiboly, involution, convergence and extension during zebrafish gastrulation. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-15T02:55:23Z (GMT). No. of bitstreams: 1 ntu-98-R96b41015-1.pdf: 8343917 bytes, checksum: 88289e17cc67c88004c62460cd092fd1 (MD5) Previous issue date: 2009 | en |
| dc.description.tableofcontents | 誌謝 i
中文摘要 ii ABSTRACT iii INTRODUCTION 1 MATERIALS AND METHODS 6 Fish maintenance and embryo collection 6 Morpholino oligonucleotides (MO) 6 Microinjection Procedures 7 Cell protrusion assay 8 Cell transplantation assay 8 Whole mount in situ hybridization (WISH) 9 Transmission electron microscopy (TEM) 9 Ventral leading edge observation 10 RESULTS 11 cfl1 tMO1 knockdown interfered with the pseudopod formation of involuting cells 11 The function of cfl1 is required cell-autonomously for cell migrations of epiboly 12 Knockdown of cfl1 caused epiboly delay with notable retardation in vegetal migration of DEL by whole mount in situ hybridization (WISH) 12 cfl1 tMO1 caused the abnormal cell detachment between EVL and DEL 13 DISCUSSION 15 cfl1 is required for pseudopod formation in the involuting cells 15 cfl1 functions cell-autonomously in the deep cell layer (DEL) 16 Knockdown of cfl1 resulted in the abnormal detachments between DEL and EVL 17 REFERENCES 19 FIGURES 23 Figure 1. Knockdown of cfl1 inhibits the pseudopod formation of involuting cells 23 Figure 2. cfl1 function is required cell-autonomously for cell migration of epiboly 24 Figure 3. Knockdown of cfl1 caused epiboly delay with notable retardation in vegetal migration of DEL 25 Figure 4. cfl1 tMO1 caused the disruption of cell attachment between EVL and DEL. 26 Figure 5. Live imaging of DEL and EVL also showed loosely contacted cell-cell interactions between DEL and EVL in cfl1 knockdown embryos. 28 APPENDIX 29 Figure 1. Sequence analysis of cofilins 30 Figure 2. cfl1 whole-mount in situ hybridization 32 Figure 3. Spatial and temporal expression pattern of cfl1 33 Figure 4. Knockdown of cfl1 causes epiboly delay in a dosage-dependent manner. 34 Figure 5. Knockdown of cfl1 inhibits convergence and extension. 35 Figure 6. Actin cytoskeleton structure of late gastrulation 37 | |
| dc.language.iso | zh-TW | |
| dc.title | Cofilin 1對斑馬魚胚原腸期細胞遷移影響之探討 | zh_TW |
| dc.title | Cofilin 1 mediates cell migrations during gastrulation in zebrafish | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 97-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 鍾邦柱(Bon-Chu Chung),劉薏雯(Yi-Wen Liu) | |
| dc.subject.keyword | 肌動蛋白去聚合蛋白,原腸期,細胞遷移,偽足,外包,包覆層,深細胞層, | zh_TW |
| dc.subject.keyword | cofilin,gastrulation,cell migration,pseudopod,epiboly,enveloping layer,deep cell layer, | en |
| dc.relation.page | 37 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2009-08-03 | |
| dc.contributor.author-college | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 動物學研究所 | zh_TW |
| Appears in Collections: | 動物學研究所 | |
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| ntu-98-1.pdf Restricted Access | 8.15 MB | Adobe PDF |
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