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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 林峰輝 | |
| dc.contributor.author | Bo-Chung Chen | en |
| dc.contributor.author | 陳柏彰 | zh_TW |
| dc.date.accessioned | 2021-06-15T02:50:27Z | - |
| dc.date.available | 2012-08-19 | |
| dc.date.copyright | 2009-08-19 | |
| dc.date.issued | 2009 | |
| dc.date.submitted | 2009-08-05 | |
| dc.identifier.citation | 1. Johla, S. S. and R. A. Burgett . Dermal filler agents: a practical review. Current Opinion in Ophthalmology.2006;17:471–479.
2. 2008 report of the 2007 statistics national clearinghouse of Plastic Surgery Statistics and 2007 Cosmetic Surgery Age Distribution. The American Society of Plastic Surgeons.2008. 3. Khor, E. Methods for the treatment of collagenous tissues for bioprostheses .Biomaterials.1997;18: 95-105. 4. 蘇玉燕、楊其嘩,生技化妝品(Bioteachnological cosmetic),高立圖書. 2005. 5. Doug Long .Skin Structure. U.S. Pharmacist .2009;1-11. 6. Wanted. Aging Skin. Bella Online.2000. 7. Cosmetic Procedures. Aging Skin Net.2003. 8. Wulf, H. C., J. Sandby-Møller, et al. Morphological and functional changes in aging skin, Micron. 2004; 35(3),3185-191. 9. P., F. and Gasparro . Sunscreens, Skin Photobiology, and Skin Cancer: The Need for UVA Protection and Evaluation of Efficacy.Environmental Health Perspectives Supplements .2000;108:s1. 10. Peter Brenneisen et al.Ultraviolet-B irradiation and matrix metalloproteinases: from induction via signaling to initial events. Ann N Y Acad Sci. 2002; 973:31-43. 11. Aging Excellence - Franchise Information .Entrepreneur.2003. 12. Barbara A. et al. Skin aging . Springer-Verlag Berlin Heidelberg;2006 13. Cheryl M. Burgess et al. Cosmetic Dermatology .Springer-Verlag Berlin Heidelberg ;2005 14. Pierard GE, Lapiere CM. The microanatomical basis of facial frown lines. Arch Dermatol. 1989;125:1090-2. 15. Fernandez, E. M., Mackley, et al. Soft tissue augmentation: a review. J Drugs Dermatol. 2006;5(7):630-41. 16. Bailey, A. J., R. G. Paul, et al. Mechanisms of maturation and ageing of collagen. Mechanisms of Ageing and Development. 1998; 106: 1-56 17. Savardekar, P. Botulinum toxin. Indian J Dermatol Venereol Leprol. 2008;74:77-9. 18. Holck DE, N. J. Facial skin rejuvenation. Curr Opin in Ophthalmol. 2003; 14:246–252. 19. N, N. and I. Y .Mechanism of amide formation by carbodiimide for bioconjugation in aqueous media. Bioconjug Chem. 1995;6(1):123-30. 20. Johla, S. S. and R. A. Burgett Dermal filler agents: a practical review. Current Opinion in Ophthalmology 2003;14:246–252. 21. WE., R. Chemical peeling in ethnic/dark skin.Dermatologic Therapy. 2004;17: 196–205. 22. Anderson, Laurence. Looking Good, the Australian guide to skin care. Cosmetic medicine and cosmetic surgery. 2006. 23. Botox® Product Summary. Consulting Room. 24. Broder, K. W. and S. R. Cohen. ArteFill®: a permanent skin filler. Expert Rev. Med. Devices. 2006;3(3): 281–289. 25. M. Eisenmann-Klein. Innovations in Plastic and Aesthetic Surgery .Springer-Verlag Berlin Heidelberg; 2008 26. Gottfried Lemperle, M. D., Ph.D., M. D. Vera Morhenn, et al. Human Histology and Persistence of Various Injectable Filler Substances for Soft Tissue Augmentation. Aesth. Plast. Surg. 2003;27:354–366. 27. Burgess CM, Lowe NJ.NewFill for skin augmentation: a new filler or failure? Dermatol Surg .2006;32:1530–1532. 28. Eckart Haneke, M. Polymethyl Methacrylate Microspheres in Collagen. Semin Cutan Med Surg. 2004;23:227-232. 29. Saylan, Z., M. Düsseldorf, et al. Facial Fillers and Their Complications. Aesthestic Surg J .2003;23:221-224. 30. Saylan, Z., M. Düsseldorf, et al. Lipografting: autologous fat grafting for total facial rejuvenation.Facial plast Surg. 2004;20: 135-143. 31. WE., R. . Chemical peeling in ethnic/dark skin. Dermatologic Therapy 2006;19: 141–150. 32. Eckart Haneke, M. Uterine natural killer cells and angiogenesis in recurrent reproductive failure. .Semin Cutan Med Surg 2006;25:122-126. 33. Leslie Baumann, M. Replacing Dermal Constituents Lost Through Aging with Dermal Fillers.Semin Cutan Med Surg .2004;23:160-166. 34. Mary P. Lupo, M. Hyaluronic Acid Fillers in Facial Rejuvenation. Semin Cutan Med Surg .2006;25:151-157. 35. Barry L. Eppley, M. D., D.M.D., et al. Injectable Soft-Tissue Fillers: Clinical Overview.Plast. Reconstr. Surg. 2006;118: 98e-106e. 36. Fernandez, E. M., Mackley, et al. Soft tissue augmentation: a review.J Drugs Dermatol. 2006;5(7):630-41 37. Prodhomme, E. J. F., A. L. Tutt, et al. Multivalent Conjugates of Poly-ç-D-glutamic Acid from Bacillus licheniformis with Antibody F(ab¢) and Glycopeptide Ligands.Bioconjugate Chem. 2003;14:1148-1155. 38. Johla, S. S. and R. A. Burgett . Dermal filler agents: a practical review.Current Opinion in Ophthalmology. 2006;17:471–479. 39. Frey, P. and S. Mangold. Physical and histological behavior of a new injectable collagen(GAX 65) implanted into the submucosal space of the mini-pig bladder.J Urol. 1995;154:812-5. 40. Chou C.H. Gelatin / hyaluronic acid / chondroitin-6-sulfate tri-copolymer sponge as the scaffold for articular cartilage tissue-engineering.Graduate Thesis.2003. 41. J.J.Wu et al. Collagen:Primer in Structure, Processing and Assembly. Springer Berlin Heidelberg New York 2005. 42. Liu H.Y. Analysis of collagen content in tissue.Graduate Thesis.2003. 43. David Shoshani, M. et al.Skin Test Hypersensitivity Study of a Cross-linked, Porcine Collagen Implant for Aesthetic Surgery. Dermatol Surg 2007;33:S152–S158. 44. 張聰民,美容藥物學, 新文京出版社.2007. 45. P. K. Smith, R. I. Krohn, G. T. Hermanson, A. K. Mallia, F. H. Gartner, M. D. Provenzano, et al. Measurement of protein using bicinchoninic acid Analytical Biochemistry 1985;150(1):76-85. 46. Eugene Khora et al.Implantable applications of chitin and chitosan. Biomaterials 2003;24:2339–2349. 47. Roche Cell Proliferation Reagent WST-1, 2005. 48. Hugo Leonardo Rocha Alves,Luis A. dos Santos,Carlos P.Bergmann：Injectability evaluation of tricalcium phosphate bone cement，Journal of Materials Science: Materials in Medicine.2008; 19:2241-2246. 49. M.P.Ginebra,A.Rilliard,E.Ferandez,C.Elvira,J.San Roman,J.A Planell：Mechanical and rheological improvement of a calcium phosphate cement by the addition of polymeric drug,John Wiley & Sons,Inc 2001;113-118. 50. Xiupeng Wang, Jiandong Ye, Hai Wang:Effects of Additives on the Rheological Properties and Injectability of a Calcium Phosphate Bone Substitute Material. Wiley InterScience 2005;259-264. 51. Yeh, H.-Y. and J.-C. Lin：Surface characterization and in vitro platelet compatibility study of surface sulfonated chitosan membrane with amino group protection-deprotection strategy. J. Biomater. Sci. Polymer Edn, 2008; 19, (3), 291–310. 52. Tan H, C. C., Payne KA, Marra KG. ：Injectable in situ forming biodegradable chitosan–hyaluronic acid based hydrogels for cartilage tissue engineering. Biomaterials 2009;30(13): 2499-506.. 53. No HK, M. S. and X. Z. Prinyawiwatkul W. 'Applications of chitosan for improvement of quality and shelf life of foods: a review.' J Food Sci Jun; 2007;72(5): R87-100. 54. Lactate Dehydrogenase (LDH) Cytotoxicity Detection Kit 55. Mikulov K, Eckhardt A, Pataridis S, Miks I. Study of posttranslational non-enzymatic modifications of collagen using capillary electrophoresis/mass spectrometry and high performance liquid chromatography/mass spectrometry. Journal of Chromatography A 2007;1155(2):125-133. 56. Patston PA, Church FC, Olson ST. Serpin-ligand interactions. Methods 2004;32(2):93-109. 57. Fernández-Cossío S, MT. C-O. Biocompatibility of two novel dermal fillers: histological evaluation of implants of a hyaluronic acid filler and a polyacrylamide filler. Plast Reconstr Surg 2006; 117(6):1789-1796. 58. Lemperle G, Morhenn VB, Pestonjamasp V, RL. G. Migration studies and histology of injectable microspheres of different sizes in mice. Plast Reconstr Surg 2004; 113(5):1380-1390. 59. Hsu S-h, Lin C-H. The properties of gelatin–poly (γ-glutamic acid) hydrogels as biological glues. Biorheology 2007; 44 17-28. 60. G. H, Welgus, K. D, Kobayashi, Jeffrey JJ. The Collagen Substrate Specificity of Rat Uterus Collagenase. The Journal of Biological Chemistry. 1983; 258(23):14162-14165. 61. Marokh et al. Enzymic characterization with progress curve analysis of a collagen peptidase from an enthomopathogenic bacterium,Photorhabdus luminescens. Biochem J 2004; 379:633-640. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/44312 | - |
| dc.description.abstract | 近年來由於生活品質的提高，人們逐漸開始注重自身的保養，由其是臉部皺紋。所以市面上出現了許多的除皺方法，不過最直接的治療方法莫過於皮下填充注射手術，該治療方法屬於不需要經過動刀即可達到除皺效果的微創手術，也目前普遍被選用來治療臉部上的皺紋。
填充材料常以自體移植、異體移植、合成材料、生物合成等等來當作填充物質，雖說有部分填充材料已經通過美國藥物食品檢驗局。但對於一個理想的填充材料而言，目前所使用的填充材料都有相當程度上的缺點。 本研究之目的在於研發出一個理想的針劑填充材料，在目前使用的材料之中以膠原蛋白對人體的生物相容性情況最為良好，可缺點在於使用期限過短，所以本研究以豬皮萃取的第一型膠原蛋白為基材，加入具生物可分解性的高分子幾丁聚醣來進行研究，企圖營造出一個理想的填充材料。 在本研究中，所製備的膠原蛋白幾丁聚醣膠體之特性將會透過流變儀、針筒注射、生物相容性、降解試驗等檢測後，可得知若以本材料為真皮填充材料則不會發生卡針的現象；且若施打進入病患體內，亦沒有材料位移的疑慮；至於降解試驗上，添加幾丁聚醣後的複合材料經本實驗證明確實可以延緩其降解結，因此若未來使用在真皮填充物及其應用上是相當具有潛力的。 | zh_TW |
| dc.description.abstract | In recent decades, facial wrinkles is becoming the embarrassed question in developed countries. These minimally invasive procedures are becoming more popular with those who want to avoid having cosmetic surgery. Dermal filler is one of minimally invasive cosmetic procedures . Dermal filler is populated to cure the fine lines、rhytids and overlying folds.
Although some injectable agents was approved by the US Food and Drug Administration(FDA) but the ideal dermal filler has continued quest . In order to find the ideal dermal filler, we use the new material, which include porcine collagen and chitosan to achieve the goal which is long-lasting soft-tissue augmentation without side effects. In this study,the collagen-chitosan gel is investigated.The cross-linking agents is glutaradehyde. For the results,the gel viscosity is increased when collagen blended with chitosan and our material may be suitable for syringe injection.For in vitro,the cytotoxicity show that collagen-chitosan gel is biocompatible.In the test of degradation, results showed that collagen blended with chitosan may slow degradation when soaking in collagenase solution.The feature of collagen-chitosan gel is potential application to dermal filler. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-15T02:50:27Z (GMT). No. of bitstreams: 1 ntu-98-R96548033-1.pdf: 2051851 bytes, checksum: 2606298e9d592c200b64302c4f9ecff7 (MD5) Previous issue date: 2009 | en |
| dc.description.tableofcontents | 國立臺灣大學碩士學位論文..................................I
中文摘要.................................................II Abstract................................................III 目錄.....................................................IV 圖目錄..................................................VII 表目錄...................................................IX 第一章 緒論..............................................1 1.1前言...................................................1 1.2 皮膚構造與生理功能....................................2 1.3皮膚衰老理論...........................................4 1.4 皺紋..................................................9 1.4.1皺紋的成因...........................................9 1.4.2 肌膚與皺紋的分類...................................10 1.4.3常見的除皺方法......................................11 1.4.4 皺紋與治療的方式...................................15 1.5 填充材料的介紹 .......................................16 1.5.1 理想的填充材料.....................................16 1.5.2 目前常見之顏面填充材料.............................17 1.6 研究動機.............................................21 第二章 基礎理論..........................................22 2.1 膠原蛋白(Collagen)...................................22 2.1.1膠原蛋白分子結構與合成 ..............................22 2.1.2第一型膠原蛋白之種類與特性..........................27 2.1.3膠原蛋白與老化......................................27 2.2幾丁聚醣的選用........................................29 2.3 交聯劑...............................................30 第三章 實驗步驟與方法....................................31 3.1實驗儀器..............................................31 3.2 實驗藥品.............................................32 3.3實驗方法與流程........................................34 3.4材料性質分析..........................................35 3.5高效能液相層析儀檢測(high performance liquid chromatography，HPLC)....................................36 3.6濃度檢測..............................................37 3.7 材料性質分析.........................................38 3.7.1膨潤試驗(Swelling ratio,SR).........................38 3.7.2材料熱性質分析(Thermal analysis)....................38 3.7.3降解試驗(Degradation)...............................39 3.7.4流變性質分析(Rheology) ..............................41 3.7.5注射試驗分析(Injectability test)....................41 3.8 生物相容性...........................................42 3.8.1 WST-1細胞活性測試(Water Soluble Tetrazolium Salt-1 Assay)...................................................42 3.8.2 LDH 乳酸脫氫酶檢定(Lactate Dehydrogenase Assay, LDH).....................................................43 第四章 結果與討論........................................45 4.1 HPLC檢測.............................................45 4.2濃度檢測..............................................48 4.3 材料性質分析.........................................50 4.3.1膨潤試驗............................................50 4.3.2流變性質分析........................................52 4.3.3材料熱性質分析......................................54 4.3.4降解試驗............................................56 4.3.5注射試驗分析........................................64 4.4生物相容性............................................65 4.4.1 WST-1細胞活性測試..................................65 4.4.2 LDH 乳酸脫氫酶檢定.................................66 第五章 結論.............................................67 參考文獻.................................................68 圖目錄 圖 1. 1皮膚結構示意圖(Diagram of Skin Structure)..........3 圖 1. 2 紫外光線與人體皮膚穿透深度關係圖.................6 圖 1. 3紫外光導致老化機制示意圖...........................6 圖 1. 4老化導致於皮膚結構變化之組織切片圖.................7 圖 1. 5真皮填充物植入點與皺紋的關係圖....................14 圖 1. 6(A) 真皮層結構變化導致細紋的產生與治療方式(B) 深層皺紋治療的方式.............................................14 圖2. 1膠原蛋白三股螺旋圖.................................23 圖2. 2生物體內膠原蛋白合成圖.............................24 圖2. 3膠原蛋白結構圖 ..............................27 圖2. 4幾丁質與幾丁聚醣...................................29 圖2. 5戊二醛交聯膠原蛋白示意圖...........................30 圖3. 1實驗流程圖.........................................35 圖3. 2 BCA試劑示意圖....................................37 圖3. 4注射器設計圖(左圖).................................41 圖3. 5 注射器實體圖(右圖)................................41 圖3. 6WST-1作用機制......................................42 圖3. 7 LDH作用機制.......................................43 圖4. 1膠原蛋白與幾丁聚醣的全波長圖譜.....................46 圖4. 2藉由HPLC分析幾丁聚醣之圖譜.........................46 圖4. 3藉由HPLC分析膠原蛋白之圖譜.........................47 圖4. 4藉由HPLC分析膠原蛋白基丁聚醣材料之圖譜.............47 圖4. 5膠原蛋白之校正曲線.................................49 圖4. 6各實驗組中膠原蛋白的含量...........................49 圖4. 7各材料於磷酸緩衝溶液中之膨潤試驗...................50 圖4. 8 各材料於磷酸緩衝溶液中之膨潤試驗..................51 圖4. 9將材料浸泡於不同溶劑之中...........................51 圖4. 10剪切應力對剪切應變的關係圖........................53 圖4. 11黏度對剪切應變的關係圖............................53 圖4. 12純材料之熱重損失與裂解溫度圖......................55 圖4. 13複合材料之熱重損失與裂解溫度圖....................55 圖4. 14膠原蛋白標準曲線圖................................56 圖4. 15不同濃度材料於磷酸緩衝溶液降解圖..................57 圖4. 16材料於磷酸緩衝溶液降解圖..........................57 圖4. 17膠原蛋白標準曲線圖................................58 圖4. 18不同濃度材料於培養基之降解圖......................59 圖4. 19材料於培養基之中降解圖............................59 圖4. 20不同濃度材料於膠原蛋白分解酶溶液中之降解圖........62 圖4. 21材料於膠原蛋白分解酶溶液中之降解圖................62 圖4. 22材料於膠原蛋白分解酶溶液中之降解圖................63 圖4. 23不同比例材料的注射性質............................64 圖4. 24 WST-1測試結果....................................65 圖4. 25 LDH測試結果......................................66 表目錄 表1. 1 2000-2005年接受軟組織填充物治療人數................1 表1. 2 皮膚病變與其成因...................................7 表1. 3老化於皮膚相關病變..................................8 表1. 4膚色分類與其特性...................................10 表1. 5以雷射治療皮膚老化之限制...........................11 表1. 6 化學脫皮法治療皺紋................................13 表1. 7目前常用填充材料整理表.............................20 | |
| dc.language.iso | zh-TW | |
| dc.subject | 交聯劑 | zh_TW |
| dc.subject | 皺皮下注射 | zh_TW |
| dc.subject | 軟組織填充物 | zh_TW |
| dc.subject | 膠原蛋白 | zh_TW |
| dc.subject | 幾丁聚醣 | zh_TW |
| dc.subject | Chitosan | en |
| dc.subject | Cross-linking agent | en |
| dc.subject | Dermal filler | en |
| dc.subject | Collagen | en |
| dc.title | 長效型膠原蛋白幾丁聚醣真皮填料 | zh_TW |
| dc.title | The Development of collagen-chitosan Dermal Filler
for long-term stability | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 97-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 姚俊旭,沙達文,莎薇塔 | |
| dc.subject.keyword | 皺皮下注射,軟組織填充物,膠原蛋白,幾丁聚醣,交聯劑, | zh_TW |
| dc.subject.keyword | Dermal filler,Collagen,Chitosan,Cross-linking agent, | en |
| dc.relation.page | 71 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2009-08-05 | |
| dc.contributor.author-college | 工學院 | zh_TW |
| dc.contributor.author-dept | 醫學工程學研究所 | zh_TW |
| 顯示於系所單位： | 醫學工程學研究所 | |
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