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標題: | 窄頻寬紅外線照射對阿拉伯芥發育及基因表現之影響 Effect of Narrow Bandwidth Infrared Radiation on Arabidopsis thaliana Growth and Gene Expression |
作者: | Su-Wei Hu 胡書緯 |
指導教授: | 李嗣涔(Si-Chen Lee) |
關鍵字: | 窄頻寬紅外線,阿拉伯芥, Narrow Bandwidth Infrared Radiation,Arabidopsis thaliana, |
出版年 : | 2009 |
學位: | 碩士 |
摘要: | 本研究的目的,是設計一種紅外線發射器,利用其所發出的窄頻寬的紅外線去照射阿拉伯芥72小時,找出某特定波段的紅外光對阿拉伯芥發芽及生長影響。本研究使用的紅外光發射器,是依據表面電漿子原理製作,此紅外光源結構是在矽基板上鍍上鉬金屬,再鍍上銀/二氧化矽/銀三層薄膜,而最上層的銀作週期排列的孔洞,藉由鉬金屬通電流加熱,此結構便會發出窄頻寬高功率的紅外光;設計出發射波長為3, 3.5, 4, 4.5, 5μm,半高寬最細可達0.5μm,加熱到160oC時發射功率可達80mW/cm2之紅外光發射器。將阿拉伯芥經過不同波長之紅外光72小時的照射後,測量其下胚軸的長度,並使用熱休克蛋白來驗證此現象非為熱效應造成,再使用北方墨點分析法分析GASA4、CHS和RbcS這三個基因之表現,便可發現不同波段的紅外光對基因表現的影響。5.0及4.0μm的紅外光照射,對阿拉伯芥的下胚軸長度分別有9.0及13.1%的抑制,3.0、3.5及4.5μm的紅外光不影響阿拉伯芥下胚軸長度,CHS和RbcS的表現量在此實驗中出現明顯差異,顯示此二基因不只受可見光誘導也會受紅外光照射所影響。 The purpose of this study is to design an infrared emitter, and utilize its narrow bandwidth infrared radiation to irradiate Arabidopsis for 72 hours, in order to identify the specific waveband of infrared radiation which may affect the seed germination and seedling development of Arabidopsis thaliana. Infrared radiation emitters used in this study are fabricated based on the principle of surface plasmon. The heat is generated by sending electric current to the molybdenum film on silicon substrate. The infrared source can be achieved by heating the triple layer structure which consists of a SiO2 layer between two Ag films on the molybdenum film. The top Ag layer is perforated by periodic holes, and the emission wavelength can be altered by changing the lattice constant and diameter of the hole arrays. In this study, plasmonic thermal emitters with different emission peak wavelengths, i.e., 3, 3.5, 4.0, 4.5, and 5.0 μm, were designed and fabricated. The smallest full width at half maximum (FWHM) could be shrunk down to 0.5μm. The highest emitting power can reach 80mW/cm2 at a temperature of 160oC. After 72 hours exposure to infrared radiation with different wavelength, the hypocotyl lengths of Arabidopsis thaliana seedlings were measured. HSP101 (heat shock protein) was used to verify that this phenomenon is not caused by thermal effects. Furthermore, Northern blot analysis is also applied to investigate the gene expression pattern of chosen genes GASA4, CHS and RbcS. Thus, different wavebands of infrared radiation affecting gene expression could be found. The infrared of wavelengths 5.0 and 4.0 μm inhibit the elongation of hypocotyl lengths by 9.0 and 13.1%, respectively. The 3.0, 3.5 and 4μm infrared irradiation do not affect the hypocotyl length of Arabidopsis thaliana. The gene expression of CHS and RbcS has significant change in this experiment, showing that the gene expressions of these two genes are not only induced by visible light but also influenced by infrared exposure. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/44310 |
全文授權: | 有償授權 |
顯示於系所單位: | 生醫電子與資訊學研究所 |
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