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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 李篤中 | |
| dc.contributor.author | Pin Lin | en |
| dc.contributor.author | 林彬 | zh_TW |
| dc.date.accessioned | 2021-06-15T02:47:25Z | - |
| dc.date.available | 2010-08-11 | |
| dc.date.copyright | 2009-08-11 | |
| dc.date.issued | 2009 | |
| dc.date.submitted | 2009-08-06 | |
| dc.identifier.citation | [1]http://www.bt.tudelft.nl/live/binaries/c771163c-7b88-476a-837d-ecb4a85bd1bb/doc/EBT_highlightGSBR.pdf
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/44255 | - |
| dc.description.abstract | 本論文探討移除活性污泥中胞外高分子物質及接種不同來源之污泥對於好氧生物顆粒化之影響。
首先分別以原始活性污泥及移除胞外高分子物質後之污泥進行接種，並利用葡萄糖作為基質碳源於連續批式反應器中培養好氧顆粒。活性污泥在移除胞外高分子物質後其好氧顆粒形成時間約為3週，原始污泥之顆粒形成時間約為2週。原始污泥所形成之顆粒有較多真菌。利用變性梯度電泳檢視兩系統之真菌相，Pseudoplatyophrya nana及Candida sp. BG02-6-6-1-7兩菌種出現在原始污泥之顆粒化初期，但並未出現在移除過胞外高分子物質之污泥其顆粒化過程中，其可能為原始污泥顆粒化較快發生的原因。兩系統之顆粒最後皆崩解，而Opisthonecta minima及Candida solani兩真菌種及Sphaerotilus sp.之細菌種皆在此時出現。 而在以乙酸進料的條件下，原始污泥及移除胞外高分子物質後之污泥其顆粒化過程則無明顯差別。以葡萄糖所培養之好氧顆粒其菌屬組成包含有：Paracoccus屬、Acidovorax屬、Pseudomonas屬等。而以乙酸所培養之好氧顆粒其菌屬組成包含有：Stenotrophomonas屬、Brevundimonas屬等。 | zh_TW |
| dc.description.abstract | Effects of removal of extracellular polymeric substances (EPS) in activated sludges on aerobic granulation process and aerobic granulation taken sluges from two wastewater treatment plants as seeds were studied.
Glucose was utilized to cultivate granules in two sequencing batch reactors (SBRs) that were seeded with original sludge (S-s) or EPS-free sludge (S-p). The S-s sludge granulated in two weeks, faster than the S-p sludge did (three weeks). The fungi were predominated in the S-s system. The denaturing gradient gel electrophoresis (DGGE) results determined that Pseudoplatyophrya nana and Candida sp. BG02-6-6-1-7 appeared in the starting phase of granulation period in S-s but not in S-p sludge, probably accounting for the earlier granulation for the former system. The fungus like Opisthonecta minima and Candida solani and bacterial species like Sphaerotilus sp. appeared in the granule disintegration phase. Acetate was utilized to cultivate granules in SBRs seeded with sludge and EPS-extracted sludge. The two seeds yielded similar granulation processes. Glucose-fed granules were composed of genus like Paracoccus、Acidovorax、Pseudomonas while acetate-fed granules were composed of Stenotrophomonas、Brevundimonas. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-15T02:47:25Z (GMT). No. of bitstreams: 1 ntu-98-R96524023-1.pdf: 12268449 bytes, checksum: bde5d6a5dd11503b11d7a4d18250bf4b (MD5) Previous issue date: 2009 | en |
| dc.description.tableofcontents | 致謝……………………………………………………………………………….……I
中文摘要………………………………………………………………………….…..II Abstract………………………………………………………………………………III 目錄…………………………………………………………………………………..IV 圖目錄………………………………………………………………………………VII 表目錄……………………………………………………………………………..…XI 第一章 前言…………………………………………………………………………1 第二章 文獻回顧……………………………………………………………………3 2-1 好氧生物顆粒............................................................................................3 2-2 連續批式反應器…………………………………………………………6 2-3 胞外高分子物質…………………………………………………….…..11 2-4 生物顆粒之形成機制…………………………………………………...12 2-5 分子生物技術於好氧生物顆粒研究的應用………………………….. 14 第三章 實驗材料與方法…………………………………………………………. 16 3-2 實驗簡述………………………………………………………………...16 3-1 連續批式反應器………………………………………………………...17 3-1-1 反應器結構…………………………………………………….17 3-1-2 連續批式反應器之參數設定………………………………….. 17 3-1-2-1 循環模式之設定………………………………………17 3-1-2-2 有機負荷率……………………………………………17 3-1-2-3 曝氣量…………………………………………………18 3-1-2-4 體積交換率……………………………………………18 3-1-2-5 水力停留時間…………………………………………18 3-2 進料組成………………………………………………………………...19 3-3 接種……………………………………………………………………...20 3-3-1 活性污泥來源…………………………………………………...20 3-3-2 胞外高分子物質之移除……………………………...…………20 3-3-3 接種…………………………………………………………..….20 3-4 分析方法……………………………………………………………...…20 3-4-1 總懸浮固體量及揮發性懸浮固體量………………………….20 3-4-2 污泥體積指數………………………………………………….20 3-4-3 溶解態胞外高分子物質………………………………….……21 3-4-3-1 胞外高分子物質之萃取……………………………..21 3-4-3-2 蛋白質定量……………………………….………….22 3-4-3-3 多醣定量……………………………………………..22 3-4-4 pH值………………………………………………………......22 3-4-5 化學需氧量……………………………………………………22 3-4-6 疏水性………………………………………………………….23 3-4-7 介達電位………………………………………………...……..23 3-4-8 粒徑分佈……………………………………….………………23 3-4-9 顯微結構觀察…………………………………………….……24 3-4-9-1 共軛聚焦顯微鏡結合多重染色法……………….….24 3-4-9-2 掃描式電子顯微鏡…………………………..………24 3-4-9-3 穿透式電子顯微鏡…………………………………..24 3-4-10 以變性梯度膠體電泳法進行菌相分析……………………...25 3-4-10-1 微生物DNA之萃取………………………………..25 3-4-10-2 聚合酶連鎖反應……………………………………25 3-4-10-3 聚合酶連鎖反應產物之純化………………………27 3-4-10-4以變性梯度膠體電泳法分析菌相……….…………27 3-4-10-4-1 細菌之變性梯度膠體電泳…………….28 3-4-10-4-2 真菌之變性梯度膠體電泳………….…28 3-4-10-4-3 核酸染色及影像檢視…………….……28 3-4-10-4-4 膠體中DNA之純化回收………...……28 3-4-10-4-5 核酸定序…………………….…………29 3-4-10-4-6 親緣演化樹分析……………….………29 3-5 單株菌種之分離及鑑定……………………………..………………….30 3-5-1 分菌…………………………………………..………………….29 3-5-2 單株菌之鑑定………………………………...…………………29 3-6 單株菌之批式搖瓶實驗………………………………………………...30 第四章 實驗結果與討論…………………………………………………………....31 4-1 以葡萄糖作為基質主要碳源培養好氧顆粒….…………………..……31 4-1-1 好氧生物顆粒化過程………………………….………………..31 4-1-2 顯微結構觀察……………………………………………..…….35 4-1-2-1 掃描式電子顯微鏡…………………………..………..35 4-1-2-2 共軛聚焦顯微鏡結合多重染色法……………………45 4-1-3 顆粒化過程中胞外高分子物質之消長…………….……..……51 4-1-3-1 蛋白質……………………………………………..…..51 4-1-3-2 多醣……………………………………………………55 4-1-3-3 蛋白質/多醣比………………………………………...57 4-1-4 疏水性及電性………………………………………………..….59 4-1-4-1 疏水性………………………………………..………..59 4-1-4-2 介達電位………………………………………………61 4-1-5 以變性梯度膠體電泳檢視顆粒化過程菌相之變化...…………62 4-1-5-1 細菌相之變化……………………..…………………..62 4-1-5-2 真菌相之變化……………………………………..…..65 4-1-6 成熟顆粒之菌種分離……………………………………….…..70 4-2 以乙酸作為基質主要碳源培養好氧顆粒…………...…………………73 4-2-1 好氧生物顆粒化過程………………………...…………………73 4-2-2 顯微結構觀察…………………………………….……………..79 4-2-2-1 掃描式電子顯微鏡……………………………………79 4-2-2-2 共軛聚焦顯微鏡結合多重染色法……………………86 4-2-3 顆粒化過程胞外高分子物質之消長…………………………...90 4-2-3-1 蛋白質……………………………………………...….90 4-2-3-2 多醣………………………..…………………………..92 4-2-3-3 蛋白質/多醣比………………………………...………95 4-2-4 以變性梯度膠體電泳檢視顆粒化過程菌相之變化…………...96 4-2-5 成熟顆粒之菌種分離………...…………………………………98 4-3 討論…………………………………………………………….………105 參考文獻…………………………………………………..…………………..……107 | |
| dc.language.iso | zh-TW | |
| dc.title | 接種污泥對連續批式反應器好氧生物顆粒化之影響 | zh_TW |
| dc.title | Effects of inoculated sludge on aerobic granulation in sequencing batch reactor | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 97-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 鄭俊華,張嘉修,黃志彬,朱曉萍 | |
| dc.subject.keyword | 好氧生物顆粒,連續批式反應器,變性梯度膠體電泳, | zh_TW |
| dc.subject.keyword | aerobic granule,SBR,DGGE, | en |
| dc.relation.page | 113 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2009-08-07 | |
| dc.contributor.author-college | 工學院 | zh_TW |
| dc.contributor.author-dept | 化學工程學研究所 | zh_TW |
| 顯示於系所單位： | 化學工程學系 | |
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