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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
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dc.contributor.advisor | 高燕玉(Yen-Yu Kao) | |
dc.contributor.author | Yi-Syuan Chen | en |
dc.contributor.author | 陳怡璇 | zh_TW |
dc.date.accessioned | 2021-06-15T02:44:49Z | - |
dc.date.available | 2009-08-14 | |
dc.date.copyright | 2009-08-14 | |
dc.date.issued | 2009 | |
dc.date.submitted | 2009-08-10 | |
dc.identifier.citation | 高玉馨. 2001. 以 5S rDNA 基因間空白區序列探討蝴蝶蘭屬植物之親緣關係. 國立台灣大學植物學研究所碩士論文.
李文靜. 2002. 蝴蝶蘭屬植物核醣體 RNA 基因的選殖及實質定位. 國立台灣大學植物學研究所碩士論文. 李益豪. 2007. 核糖體 RNA 基因於七種蝴蝶蘭屬植物的分佈. 國立台灣大學分子與細胞生物學研究所碩士論文. 彭欣羚. 2007. 利用數種重複性 DNA 序列的分佈建立朵麗蘭的核型.國立台灣大學分子與細胞生物學研究所碩士論文. 曾治中. 2009. 三種蝴蝶蘭屬植物核糖體 RNA 基因的實質定位. 國立台灣大學分子與細胞生物學研究所碩士論文. Adams, S. P., I. J. Leitch, M. D. Bennett, M. W. Chase, and A. R. Leitch.2000. Ribosomal DNA evolution and phylogeny in Aloe (Asphodelaceae). Am. J. Bot. 87: 1578-1583. Arends, J. C. 1970. Cytological observations on genome homology in eight interspecific hybrids of Phalaenopsis. Genetica 41: 88-100. Ausubel, F. M., R. Brent, D. D. More, J. G. Sediman, J. A. Smith, and K. Struhl. 1989. Current Protocols in Molecular Biology. Greene Publishing Associates and Wile- Interscience USA. Avramova, Z. V. 2002. Heterochromatin in animals and plants. Similarities and differences. Plant Physiol. 129: 40-49. Baldwin, B. G., M. J. Sanderson, J. M. Porter, M. F. Wojciechowski, C. S. Campbell, and M. J. Donoghue. 1995. The ITS region of nuclear ribosomal DNA: a valuable source of evidence on angiosperm phylogeny. Ann. Mo. Bot. Gard. 82: 247-277. Bartos, J., O. Alkhimova, M. Dolezelova, E. D. Langhe, and J. Dolezel. 2005. Nuclear genome size and genomic distribution of ribosomal DNA in Musa and Ensete (Musaceae): taxonomic implications. Cytogenet. Genome Res. 109: 50-57. Choi, Y. A., R. Tao, K. Yonemori, and A. Sugiura. 2003. Physical mapping of 45S rDNA by fluorescent in situ hybridization in persimmon (Diospyros kaki) and its wild relatives. J. Hort. Sci. Biotech. 78: 265-271. Christenson, E. A. 2001. Phalaenopsis: a monograph. Timber Press, Portland, Oregon. Chung, M. C., Y. I. Lee, Y. Y. Cheng, Y. J. Chou, and C. F. Lu. 2008. Chromosomal polymorphism of ribosomal genes in the genus Oryza. Theor. Appl. Genet. 116: 745-753. Cox, A. V., A. M. Pridgeon, V. A. Albert, and M. W. Chase. 1997. Phylogenetics of the slipper orchids (Cypripedioideae, Orchidaceae): nuclear rDNA ITS sequences. Plant Syst. Evol. 208:197-223. Dhar, M. K., B. Friebe, S. Kaul, and B. S. Gill. 2006. Characterization and physical mapping of ribosomal RNA gene families in Plantago. Ann. Bot. 97: 541-548. Dressler, R. L. 1993. Phylogeny and classification of the orchid family . Cambridge University Press, Cambridge. Gurushidze, M., S. Mashayekhi, F. R. Blattner, N. Friesen, and R. M. Fritsch. 2007. Phylogenetic relationships of wild and cultivated species of Allium section Cepa inferred by nuclear rDNA ITS sequence analysis. Plant Syst. Evol. 269: 259-269. Jiang, J., and B. S. Gill. 1994. Nonisotopic in situ hybridization and plant genome mapping: the first 10 years. Genome 37: 717-725. Kamemoto, H., R. Tanaka, and K. Kosaki. 1961. Chromsome numbers of orchids in Hawaii. Hawaii Agricultural Experiment Station Bull. 127: 1-27. Kao, Y. Y., S. B. Chang, T. Y. Lin, C. H. Hsieh, Y. H. Chen, W. H. Chen, and C. C. Chen. 2001. Differential accumulation of heterochromatin as a cause for karyotype variation in Phalaenopsis orchids. Ann. Bot. 87: 387-395. Kato, A., J. C. Lamb, and J. A. Birchler. 2004. Chromosome painting using repetitive DNA sequences as probes for somatic chromosome identification in maize. Proc. Natl. Acad. Sci. 101: 13554-13559. Lapitan, N. L. V. 1992. Organization and evolution of higher plant nuclear genomes. Genome 35: 171-181. Lim, K. Y., A. Kovarik, R. Matyasek, M. W. Chase, S. Knapp, E. McCarthy, J. J. Clarkson, and A. R. Leitch. 2006. Comparative genomics and repetitive sequence divergence in the species of diploid Nicotiana section Alatae. Plant J. 48: 907-919. Lin, S., H. C. Lee, W. H. Chen, C. C. Chen, Y. Y. Kao, Y. M. Fu, Y. H. Chen, and T. Y. Lin. 2001. Nuclear DNA contents of Phalaenopsis sp. and Doritis pulcherrima. J. Am. Soc. Hortic. Sci. 126: 195-199. Lin, T. C., C. C. Hsieh, C. L. Kuo, F. S. Chueh, and H. S. Tsay. 2007. ITS sequence based phylogenetic relationship of Dangshen Radix. J. Food Drug Anal. 15: 428-432. Meagher, T. R. and C. Vassiliadis. 2005. Phenotypic impacts of repetitive DNA in flowering plants. New Phytol. 168: 71-80. Murata, M., J. S. Heslop-Harrison, and F. Motoyoshi. 1997. Physical mapping of the 5S ribosomal RNA genes in Arabidopsis thaliana by multi-color fluorescence in situ hybridization with cosmid clones. Plant J. 12: 31-37. Panaud, O., G. Magpantay, and S. McCouch. 1993. A protocol for nonradioactive DNA labeling and detection in the RFLP analysis of rice and tomato using single-copy probes. Plant Mol. Bio. Rep. 11:54-59. Rogers, S. O., and A. J. Bendich. 1987. Ribosomal RNA genes in plants:variability in copy number and in the intergenic spacer. Plant Mol. Biol. 9: 509-520. Ruas, C. F., A. L. L. Vanzela, M. O. Santos, J. N. Fregonezi, P. M. Ruas, N. I. Matzenbacher, and M. L. R. de Aguiar-Perecin. 2005. Chromosomal organization and phylogenetic relationships in Hypochaeris species (Asteraceae) from Brazil. Genet. Mol. Biol. 28: 1129-1139. Sagawa, Y. 1962. Cytological studies on the genus Phalaenopsis. Am. Orchid Soc. Bull. 31: 459-465. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular Cloning : a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York. Schwarzacher, T., and J. S. Heslop-Harrison. 2000. Practical in situ hybridization. BIOS Scientific Publishers, Oxford. Shindo, K., and H. Kamemoto. 1963. Karyotype analysis of some species of Phalaenopsis. Cytologia 28: 390-398. Smith, G. P. 1976. Evolution of repeated DNA sequences by unequal crossover. Science 191: 528-535. Sweet, H. R. 1980. The genus Phalaenopsis. Day Printing Corp., Pomon, California. Tsai, C. C., S. C. Huang, and C. H. Chou. 2006. Molecular phylogeny of Phalaenopsis Blume (Orchidaceae) based on the internal transcribed spacer of the nuclear ribosomal DNA. Plant Syst. Evol. 256: 1-16. Tsai, C. C., C. I. Peng, S. C. Huang, P. L. Huang, and C. H. Chou. 2004. Determination of the genetic relationship of Dendrobium species (Orchidaceae) in Taiwan based on the sequence of the internal transcribed spacer of ribosomal DNA. Sci. Hortic. 101: 315-325. Woodard, J. W. 1951. Some chromosome numbers in Phalaenopsis. Am. Orchid Soc. Bull. 20: 356-358. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/44204 | - |
dc.description.abstract | 五種蝴蝶蘭原生種 P. amabilis 、 P. aphrodite 、 P. bastianii 、 P. pulchra 及 P. tetraspis 皆具 38 條染色體 (2n = 2x = 38) ,但染色體大小有差異。利用螢光原位雜交將兩種 rDNA 定位於染色體上,屬於 Phalaenopsis 節之 P. amabilis 及 P. aphrodite 皆具 1 對 45S rDNA 基因座及 2 對 5S rDNA 基因座,但前者之45S rDNA 基因座具多型性且 2 對 5S rDNA 基因座均位於染色體末端,而後者有1 對 5S rDNA 基因座位於染色體末端,另 1 對 5S rDNA 基因座則位於染色體臂間且具多型性。屬於 Amboinenses 節之 P. bastianii 及 P. pulchra 皆具 1 對 45S rDNA 基因座及 1 對 5S rDNA 基因座,與同節之P. luddemanniana 、 P. fasciata 、 P. mariae 及 P. pallens 相
同,但卻與 P. amboinensis 、 P. venosa 及 P. violacea 分別具 4 ~ 7 對 45S rDNA 基因座及 1 ~ 2 對 5S rDNA 基因座有明顯差異。此外,兩群植物在染色體形態和 DNA 含量亦有很大不同,與利用 5S rDNA 的 IGS 序列以及 45S rDNA 的 ITS 序列所建立之親緣關係樹將此九種蝴蝶蘭分為兩群的結果相符。屬於 Zebrinae 節之 P. tetraspis 具有 1 對 5S rDNA 基因座及 5 對 45S rDNA 基因座,且 45S rDNA 大都位於小染色體上,與含基因組較大的蝴蝶蘭 P. amboinensis、P. venosa、P. violacea 及 P. pulcherrima 有類似的結果,此種特殊分佈是值得探討之問題。 | zh_TW |
dc.description.abstract | Phalaenopsis amabilis, P. aprodite, P. bastianii, P. pulchra, and P. tetraspis had 38 chromosomes (2n = 2x = 38), but their chromosome size differed. Fluorescence in situ hybridization was used for physical mapping of 5S and 45S rDNAs. P. amabilis and P. aphrodite belonging to the section Phalaenopsis had one pair of 45S rDNA loci and two pairs of 5S rDNA loci. The former had polymorphic 45S rDNA loci and two pairs of the 5S rDNA loci at the terminal site, whereas the latter contained one pair of 5S rDNA loci at the terminal site and the other pair at the interstitial region with polymorphism. Both P. bastianii and P. pulchra of the section Amboinenses possessed one pair of 45S rDNA loci and one pair of 5S rDNA loci. In the same section, P. luddemanniana, P. fasciata, P. mariae and P. pallens not only had the same number of rDNA loci, but
also the chromosome size and DNA content were the same as these two species. On the contrary, P. amboinensis, P. venosa and P. violacea had four to seven pairs of 45S rDNA loci and one to two pairs of 5S rDNA loci. In addition, these three species contained larger chromosomes and higher DNA contents. Therefore, these nine species could be divided into two groups. The results were consistent with the phylogenetic tree constructed with IGS region of 5S rDNA and ITS region of 45S rDNA. P. tetraspis classified to the section Zebrinae contained one pair of 5S rDNA loci and five pairs of 45S rDNA loci. The 45S rDNA loci were almost located in the small chromosomes. The similar distribution of 45S rDNA was also observed in the species with larger genomes, such as P. amboinensis, P. venosa, P. violacea, and P. pulcherrima. Further studies are needed to clarify this special phenomenon. | en |
dc.description.provenance | Made available in DSpace on 2021-06-15T02:44:49Z (GMT). No. of bitstreams: 1 ntu-98-R95b43032-1.pdf: 1118393 bytes, checksum: ba68aba9e0531e24f506c0c299cceb1a (MD5) Previous issue date: 2009 | en |
dc.description.tableofcontents | 目錄
中文摘要--------------------------------------------------i 英文摘要-------------------------------------------------ii 壹、前言--------------------------------------------------1 貳、材料方法 一、植物材料------------------------------------------8 二、根尖染色體的觀察----------------------------------8 三、含 rDNA 之質體的抽取------------------------------8 四、探針之標定---------------------------------------10 五、螢光原位雜交-------------------------------------13 參、結果 一、染色體的觀察-------------------------------------19 二、螢光原位雜交-------------------------------------19 肆、討論-------------------------------------------------27 伍、參考文獻---------------------------------------------33 | |
dc.language.iso | zh-TW | |
dc.title | 五種蝴蝶蘭屬植物核糖體 RNA 基因之染色體定位 | zh_TW |
dc.title | Chromosome localization of ribosomal RNA genes in five Phalaenopsis species | en |
dc.type | Thesis | |
dc.date.schoolyear | 97-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 吳文鑾,鍾美珠 | |
dc.subject.keyword | 螢光原位雜交,45S rDNA,5S rDNA, | zh_TW |
dc.subject.keyword | fluorescence in situ hybridization,45S rDNA,5S rDNA, | en |
dc.relation.page | 38 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2009-08-10 | |
dc.contributor.author-college | 生命科學院 | zh_TW |
dc.contributor.author-dept | 分子與細胞生物學研究所 | zh_TW |
顯示於系所單位: | 分子與細胞生物學研究所 |
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