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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 林俊宏(Chun-Hung Lin) | |
| dc.contributor.author | Chun Lo | en |
| dc.contributor.author | 羅鈞 | zh_TW |
| dc.date.accessioned | 2021-06-15T02:40:50Z | - |
| dc.date.available | 2016-09-08 | |
| dc.date.copyright | 2011-09-08 | |
| dc.date.issued | 2011 | |
| dc.date.submitted | 2011-08-16 | |
| dc.identifier.citation | 1. Greenwell, P. (1997) Glycoconj. J. 14, 159-173.
2. Cordero, O. J., Merino, A., Paez de la Cadena, M., Bugia, B., Nogueira, M., Vinuela, J. E., Martinez-Zorzano, V. S., de Carlos, A., and Rodriguez-Berrocal, F. J. (2001) Eur. J. Biochem. 268, 3321-3331. 3. Alhadeff, J. A., Khunsook, S., Choowongkomon, K., Baney, T., Heredia, V., Tweedie, A., and Bean, B. (1999) Mol. Hum. Reprod. 5, 809-815. 4. Aspholm-Hurtig, M., Dailide, G., Lahmann, M., Kalia, A., Ilver, D., Roche, N., Vikstrom, S., Sjostrom, R., Linden, S., Backstrom, A., Lundberg, C., Arnqvist, A., Mahdavi, J., Nilsson, U. J., Velapatino, B., Gilman, R. H., Gerhard, M., Alarcon, T., Lopez-Brea, M., Nakazawa, T., Fox, J. G., Correa, P., Dominguez-Bello, M. G., Perez-Perez, G. I., Blaser, M. J., Normark, S., Carlstedt, I., Oscarson, S., Teneberg, S., Berg, D. E., and Boren, T. (2004) Science 305, 519-522. 5. Becker, D. J., and Lowe, J. B. (1999) Biochim. Biophys. Acta 1455, 193-204. 6. Tarling, C. A., He, S., Sulzenbacher, G., Bignon, C., Bourne, Y., Henrissat, B., and Withers, S. G. (2003) J. Biol. Chem. 278, 47394-47399. 7. Lucas, H., Bercegeay, S., Le Pendu, J., Jean, M., Mirallie, S., and Barriere, P. (1994) Hum. Reprod. 9, 1532-1538. 8. Khunsook, S., Bean, B. S., McGowan, S. R., and Alhadeff, J. A. (2003) Biol. Reprod. 68, 709-716. 9. Abdel-Aleem, H., Ahmed, A., Sabra, A. M., Zakhari, M., Soliman, M., and Hamed, H. (1996) Int. J. Gynaecol. Obstet. 55, 273-279. 10. Ayude, D., Fernandez-Rodriguez, J., Rodriguez-Berrocal, F. J., Martinez-Zorzano, V. S., de Carlos, A., Gil, E., and Paez de La Cadena, M. (2000) Oncology 59, 310-316. 11. Giardina, M. G., Matarazzo, M., Morante, R., Lucariello, A., Varriale, A., Guardasole, V., and De Marco, G. (1998) Cancer 83, 2468-2474. 12. Yuan, K., Kucik, D., Singh, R. K., Listinsky, C. M., Listinsky, J. J., and Siegal, G. P. (2008) Int. J. Oncol. 32, 797-807. 13. Shah, M., Telang, S., Raval, G., Shah, P., and Patel, P. S. (2008) Cancer 113, 336-346. 14. Hutchinson, W. L., Johnson, P. J., Du, M. Q., and Williams, R. (1991) Clin. Sci. (Lond) 81, 177-182. 15. Sano, M., Hayakawa, K., and kato, I. (1992) J. Biol. Chem. 267, 1522-1527. 16. Butters, T. D., Scudder, P., Rotsaert, J., Petursson, S., Fleet, G. W., Willenbrock, F. W., and Jacob, G. S. (1991) Biochem. J. 279 ( Pt 1), 189-195. 17. Johnson, S. W., and Alhadeff, J. A. (1991) Comp. Biochem. Physiol. B 99, 479-488. 18. Vanhooren, P. T., and Vandamme, E. J. (1999) J. Chem. Technol. Biotechnol. 74, 479-497. 19. Nagae, M., Tsuchiya, A., Katayama, T., Yamamoto, K., Wakatsuki, S., and Kato, R. (2007) J. Biol. Chem. 282, 18497-18509. 20. Hidaka, M., Fushinobu, S., Honda, Y., Wakagi, T., Shoun, H., and Kitaoka, M. (2010) J. Biochem. 147, 237-244. 21. Henrissat, B. (1991) Biochem. J. 280 ( Pt 2), 309-316. 22. White, W. J., Schray, K. J., Legler, G., and Alhadeff, J. A. (1987) Biochim. Biophys. Acta. 912, 132-138. 23. Cobucci-Ponzano, B., Trincone, A., Giordano, A., Rossi, M., and Moracci, M. (2003) Biochemistry 42, 9525-9531. 24. Eneyskaya, E. V., Kulminskaya, A. A., Kalkkinen, N., Nifantiev, N. E., Arbatskii, N. P., Saenko, A. I., Chepurnaya, O. V., Arutyunyan, A. V., Shabalin, K. A., and Neustroev, K. N. (2001) Glycoconj. J. 18, 827-834. 25. Intra, J., Perotti, M. E., Pavesi, G., and Horner, D. (2007) Gene 392, 34-46. 26. Alhadeff, J. A., Miller, A. L., Wenaas, H., Vedvick, T., and O'Brien, J. S. (1975) J. Biol. Chem. 250, 7106-7113. 27. Watkins, P., and Alhadeff, J. A. (1981) Comp. Biochem. Physiol. B. Biochem. Mol. Biol. 68, 509-516. 28. DiCioccio, R. A., Barlow, J. J., and Matta, K. L. (1982) J. Biol. Chem. 257, 714-718. 29. Hopfer, R. L., Johnson, S. W., Masserini, M., Giuliani, A., and Alhadeff, J. A. (1990) Biochem. J. 266, 491-496. 30. Li, Y. K., and Liu, S. W. (2009) J. Chin. Chem. Soc. (Taipei, Taiwan) 56, 850-858. 31. Liu, T. W., Ho, C. W., Huang, H. H., Chang, S. M., Popat, S. D., Wang, Y. T., Wu, M. S., Chen, Y. J., and Lin, C. H. (2009) Proc. Natl. Acad. Sci. U. S. A. 106, 14581-14586. 32. Willems, P. J., Seo, H. C., Coucke, P., Tonlorenzi, R., and O'Brien, J. S. (1999) Eur. J. Hum. Genet. 7, 60-67. 33. Ip, P., Goh, W., Chan, K. W., and Cheung, P. T. (2002) J. Inherit. Metab. Dis. 25, 415-416. 34. de Carlos, A., Montenegro, D., Alonso-Rodriguez, A., Paez de la Cadena, M., Rodriguez-Berrocal, F. J., and Martinez-Zorzano, V. S. (2003) J. Chromatogr. B Analyt. Technol. Biomed. Life. Sci. 786, 7-15. 35. Liu, S. W., Chen, C. S., Chang, S. S., Mong, K. K., Lin, C. H., Chang, C. W., Tang, C. Y., and Li, Y. K. (2009) Biochemistry 48, 110-120. 36. Mungall, A. J., Palmer, S. A., Sims, S. K., Edwards, C. A., Ashurst, J. L., Wilming, L., Jones, M. C., Horton, R., Hunt, S. E., Scott, C. E., Gilbert, J. G., Clamp, M. E., Bethel, G., Milne, S., Ainscough, R., Almeida, J. P., Ambrose, K. D., Andrews, T. D., Ashwell, R. I., Babbage, A. K., Bagguley, C. L., Bailey, J., Banerjee, R., Barker, D. J., Barlow, K. F., Bates, K., Beare, D. M., Beasley, H., Beasley, O., Bird, C. P., Blakey, S., Bray-Allen, S., Brook, J., Brown, A. J., Brown, J. Y., Burford, D. C., Burrill, W., Burton, J., Carder, C., Carter, N. P., Chapman, J. C., Clark, S. Y., Clark, G., Clee, C. M., Clegg, S., Cobley, V., Collier, R. E., Collins, J. E., Colman, L. K., Corby, N. R., Coville, G. J., Culley, K. M., Dhami, P., Davies, J., Dunn, M., Earthrowl, M. E., Ellington, A. E., Evans, K. A., Faulkner, L., Francis, M. D., Frankish, A., Frankland, J., French, L., Garner, P., Garnett, J., Ghori, M. J., Gilby, L. M., Gillson, C. J., Glithero, R. J., Grafham, D. V., Grant, M., Gribble, S., Griffiths, C., Griffiths, M., Hall, R., Halls, K. S., Hammond, S., Harley, J. L., Hart, E. A., Heath, P. D., Heathcott, R., Holmes, S. J., Howden, P. J., Howe, K. L., Howell, G. R., Huckle, E., Humphray, S. J., Humphries, M. D., Hunt, A. R., Johnson, C. M., Joy, A. A., Kay, M., Keenan, S. J., Kimberley, A. M., King, A., Laird, G. K., Langford, C., Lawlor, S., Leongamornlert, D. A., Leversha, M., Lloyd, C. R., Lloyd, D. M., Loveland, J. E., Lovell, J., Martin, S., Mashreghi-Mohammadi, M., Maslen, G. L., Matthews, L., McCann, O. T., McLaren, S. J., McLay, K., McMurray, A., Moore, M. J., Mullikin, J. C., Niblett, D., Nickerson, T., Novik, K. L., Oliver, K., Overton-Larty, E. K., Parker, A., Patel, R., Pearce, A. V., Peck, A. I., Phillimore, B., Phillips, S., Plumb, R. W., Porter, K. M., Ramsey, Y., Ranby, S. A., Rice, C. M., Ross, M. T., Searle, S. M., Sehra, H. K., Sheridan, E., Skuce, C. D., Smith, S., Smith, M., Spraggon, L., Squares, S. L., Steward, C. A., Sycamore, N., Tamlyn-Hall, G., Tester, J., Theaker, A. J., Thomas, D. W., Thorpe, A., Tracey, A., Tromans, A., Tubby, B., Wall, M., Wallis, J. M., West, A. P., White, S. S., Whitehead, S. L., Whittaker, H., Wild, A., Willey, D. J., Wilmer, T. E., Wood, J. M., Wray, P. W., Wyatt, J. C., Young, L., Younger, R. M., Bentley, D. R., Coulson, A., Durbin, R., Hubbard, T., Sulston, J. E., Dunham, I., Rogers, J., and Beck, S. (2003) Nature 425, 805-811. 37. Venter, J. C., Adams, M. D., Myers, E. W., Li, P. W., Mural, R. J., Sutton, G. G., Smith, H. O., Yandell, M., Evans, C. A., Holt, R. A., Gocayne, J. D., Amanatides, P., Ballew, R. M., Huson, D. H., Wortman, J. R., Zhang, Q., Kodira, C. D., Zheng, X. H., Chen, L., Skupski, M., Subramanian, G., Thomas, P. D., Zhang, J., Gabor Miklos, G. L., Nelson, C., Broder, S., Clark, A. G., Nadeau, J., McKusick, V. A., Zinder, N., Levine, A. J., Roberts, R. J., Simon, M., Slayman, C., Hunkapiller, M., Bolanos, R., Delcher, A., Dew, I., Fasulo, D., Flanigan, M., Florea, L., Halpern, A., Hannenhalli, S., Kravitz, S., Levy, S., Mobarry, C., Reinert, K., Remington, K., Abu-Threideh, J., Beasley, E., Biddick, K., Bonazzi, V., Brandon, R., Cargill, M., Chandramouliswaran, I., Charlab, R., Chaturvedi, K., Deng, Z., Di Francesco, V., Dunn, P., Eilbeck, K., Evangelista, C., Gabrielian, A. E., Gan, W., Ge, W., Gong, F., Gu, Z., Guan, P., Heiman, T. J., Higgins, M. E., Ji, R. R., Ke, Z., Ketchum, K. A., Lai, Z., Lei, Y., Li, Z., Li, J., Liang, Y., Lin, X., Lu, F., Merkulov, G. V., Milshina, N., Moore, H. M., Naik, A. K., Narayan, V. A., Neelam, B., Nusskern, D., Rusch, D. B., Salzberg, S., Shao, W., Shue, B., Sun, J., Wang, Z., Wang, A., Wang, X., Wang, J., Wei, M., Wides, R., Xiao, C., Yan, C., Yao, A., Ye, J., Zhan, M., Zhang, W., Zhang, H., Zhao, Q., Zheng, L., Zhong, F., Zhong, W., Zhu, S., Zhao, S., Gilbert, D., Baumhueter, S., Spier, G., Carter, C., Cravchik, A., Woodage, T., Ali, F., An, H., Awe, A., Baldwin, D., Baden, H., Barnstead, M., Barrow, I., Beeson, K., Busam, D., Carver, A., Center, A., Cheng, M. L., Curry, L., Danaher, S., Davenport, L., Desilets, R., Dietz, S., Dodson, K., Doup, L., Ferriera, S., Garg, N., Gluecksmann, A., Hart, B., Haynes, J., Haynes, C., Heiner, C., Hladun, S., Hostin, D., Houck, J., Howland, T., Ibegwam, C., Johnson, J., Kalush, F., Kline, L., Koduru, S., Love, A., Mann, F., May, D., McCawley, S., McIntosh, T., McMullen, I., Moy, M., Moy, L., Murphy, B., Nelson, K., Pfannkoch, C., Pratts, E., Puri, V., Qureshi, H., Reardon, M., Rodriguez, R., Rogers, Y. H., Romblad, D., Ruhfel, B., Scott, R., Sitter, C., Smallwood, M., Stewart, E., Strong, R., Suh, E., Thomas, R., Tint, N. N., Tse, S., Vech, C., Wang, G., Wetter, J., Williams, S., Williams, M., Windsor, S., Winn-Deen, E., Wolfe, K., Zaveri, J., Zaveri, K., Abril, J. F., Guigo, R., Campbell, M. J., Sjolander, K. V., Karlak, B., Kejariwal, A., Mi, H., Lazareva, B., Hatton, T., Narechania, A., Diemer, K., Muruganujan, A., Guo, N., Sato, S., Bafna, V., Istrail, S., Lippert, R., Schwartz, R., Walenz, B., Yooseph, S., Allen, D., Basu, A., Baxendale, J., Blick, L., Caminha, M., Carnes-Stine, J., Caulk, P., Chiang, Y. H., Coyne, M., Dahlke, C., Mays, A., Dombroski, M., Donnelly, M., Ely, D., Esparham, S., Fosler, C., Gire, H., Glanowski, S., Glasser, K., Glodek, A., Gorokhov, M., Graham, K., Gropman, B., Harris, M., Heil, J., Henderson, S., Hoover, J., Jennings, D., Jordan, C., Jordan, J., Kasha, J., Kagan, L., Kraft, C., Levitsky, A., Lewis, M., Liu, X., Lopez, J., Ma, D., Majoros, W., McDaniel, J., Murphy, S., Newman, M., Nguyen, T., Nguyen, N., Nodell, M., Pan, S., Peck, J., Peterson, M., Rowe, W., Sanders, R., Scott, J., Simpson, M., Smith, T., Sprague, A., Stockwell, T., Turner, R., Venter, E., Wang, M., Wen, M., Wu, D., Wu, M., Xia, A., Zandieh, A., and Zhu, X. (2001) Science 291, 1304-1351. 38. Lamesch, P., Li, N., Milstein, S., Fan, C., Hao, T., Szabo, G., Hu, Z., Venkatesan, K., Bethel, G., Martin, P., Rogers, J., Lawlor, S., McLaren, S., Dricot, A., Borick, H., Cusick, M. E., Vandenhaute, J., Dunham, I., Hill, D. E., and Vidal, M. (2007) Genomics 89, 307-315. 39. Lloyd, K. O. (1987) Am. J. Clin. Pathol. 87, 129-139. 40. Sakamoto, S., Watanabe, T., Tokumaru, T., Takagi, H., Nakazato, H., and Lloyd, K. O. (1989) Cancer Res. 49, 745-752. 41. Saadi, A. T., Blackwell, C. C., Raza, M. W., James, V. S., Stewart, J., Elton, R. A., and Weir, D. M. (1993) Epidemiol. Infect. 110, 507-517. 42. Heneghan, M. A., Moran, A. P., Feeley, K. M., Egan, E. L., Goulding, J., Connolly, C. E., and McCarthy, C. F. (1998) FEMS Immunol. Med. Microbiol. 20, 257-266. 43. Norman, K. E., Anderson, G. P., Kolb, H. C., Ley, K., and Ernst, B. (1998) Blood 91, 475-483. 44. Zhang, B. H., Chen, H., Yao, X. P., Cong, W. M., and Wu, M. C. (2002) Hepatobiliary Pancreat. Dis. Int. 1, 80-82. 45. Linden, S., Boren, T., Dubois, A., and Carlstedt, I. (2004) Biochem. J. 379, 765-775. 46. Campbell, B. J., Rogerson, K. A., and Rhodes, J. M. (1999) Gastroenterology 116, A131-A131. 47. Boren, T., Falk, P., Roth, K. A., Larson, G., and Normark, S. (1993) Science 262, 1892-1895. 48. Mahdavi, J., Sonden, B., Hurtig, M., Olfat, F. O., Forsberg, L., Roche, N., Angstrom, J., Larsson, T., Teneberg, S., Karlsson, K. A., Altraja, S., Wadstrom, T., Kersulyte, D., Berg, D. E., Dubois, A., Petersson, C., Magnusson, K. E., Norberg, T., Lindh, F., Lundskog, B. B., Arnqvist, A., Hammarstrom, L., and Boren, T. (2002) Science 297, 573-578. 49. Monteiro, M. A., Zheng, P., Ho, B., Yokota, S., Amano, K., Pan, Z., Berg, D. E., Chan, K. H., MacLean, L. L., and Perry, M. B. (2000) Glycobiology 10, 701-713. 50. Monteiro, M. A., Chan, K. H., Rasko, D. A., Taylor, D. E., Zheng, P. Y., Appelmelk, B. J., Wirth, H. P., Yang, M., Blaser, M. J., Hynes, S. O., Moran, A. P., and Perry, M. B. (1998) J. Biol. Chem. 273, 11533-11543. 51. Rasko, D. A., Wilson, T. J., Zopf, D., and Taylor, D. E. (2000) J. Infect. Dis. 181, 1089-1095. 52. Weis, W. I., Taylor, M. E., and Drickamer, K. (1998) Immunol. Rev. 163, 19-34. 53. Zheng, P. Y., Hua, J., Yeoh, K. G., and Ho, B. (2000) Gut 47, 18-22. 54. Heneghan, M. A., McCarthy, C. F., and Moran, A. P. (2000) Infect. Immun. 68, 937-941. 55. Martin, S. L., McColm, A. A., and Appelmelk, B. J. (2000) Gastroenterology 119, 1414-1416. 56. Edwards, N. J., Monteiro, M. A., Faller, G., Walsh, E. J., Moran, A. P., Roberts, I. S., and High, N. J. (2000) Mol. Microbiol. 35, 1530-1539. 57. Guruge, J. L., Falk, P. G., Lorenz, R. G., Dans, M., Wirth, H. P., Blaser, M. J., Berg, D. E., and Gordon, J. I. (1998) Proc. Natl. Acad. Sci. U. S. A. 95, 3925-3930. 58. Covacci, A., and Rappuoli, R. (2000) J. Exp. Med. 191, 587-592. 59. Wirth, H. P., Yang, M., Karita, M., and Blaser, M. J. (1996) Infect. Immun. 64, 4598-4605. 60. Falgueyret, J. P., Black, W. C., Cromlish, W., Desmarais, S., Lamontagne, S., Mellon, C., Riendeau, D., Rodan, S., Tawa, P., Wesolowski, G., Bass, K. E., Venkatraman, S., and Percival, M. D. (2004) Anal. Biochem. 335, 218-227. 61. Timmer, M. S., Stocker, B. L., and Seeberger, P. H. (2007) Curr. Opin. Chem. Biol. 11, 59-65. 62. Saghatelian, A., Jessani, N., Joseph, A., Humphrey, M., and Cravatt, B. F. (2004) Proc. Natl. Acad. Sci. U. S. A. 101, 10000-10005. 63. Kwan, D. H., Chen, H. M., Ratananikom, K., Hancock, S. M., Watanabe, Y., Kongsaeree, P. T., Samuels, A. L., and Withers, S. G. (2011) Angew. Chem. Int. Ed. 50, 300-303. 64. Witte, M. D., Kallemeijn, W. W., Aten, J., Li, K. Y., Strijland, A., Donker-Koopman, W. E., van den Nieuwendijk, A. M., Bleijlevens, B., Kramer, G., Florea, B. I., Hooibrink, B., Hollak, C. E., Ottenhoff, R., Boot, R. G., van der Marel, G. A., Overkleeft, H. S., and Aerts, J. M. (2010) Nat. Chem. Biol. 6, 907-913. 65. Williams, S. J., Hekmat, O., and Withers, S. G. (2006) ChemBioChem 7, 116-124. 66. Vocadlo, D. J., and Bertozzi, C. R. (2004) Angew. Chem. Int. Ed. Engl. 43, 5338-5342. 67. Ichikawa, M., and Ichikawa, Y. (2001) Bioorg. Med. Chem. Lett. 11, 1769-1773. 68. Sisk, W. N., Ono, N., Yano, T., and Wada, M. (2002) Dyes Pigm. 55, 143-150. 69. Lim, S. H., Thivierge, C., Nowak-Sliwinska, P., Han, J., van den Bergh, H., Wagnieres, G., Burgess, K., and Lee, H. B. (2010) J. Med. Chem. 53, 2865-2874. 70. Ho, C. W., Lin, Y. N., Chang, C. F., Li, S. T., Wu, Y. T., Wu, C. Y., Liu, S. W., Li, Y. K., and Lin, C. H. (2006) Biochemistry 45, 5695-5702. 71. Dawson, G., and Tsay, G. (1977) Arch. Biochem. Biophys. 184, 12-23. 72. Conzelmann, E., Burg, J., Stephan, G., and Sandhoff, K. (1982) Eur. J. Biochem. 123, 455-464. 73. Warren, J. R. (1983) Lancet 1, 1273-1273. 74. Marshall, B. J., and Warren, J. R. (1984) Lancet 1, 1311-1315. 75. Drumm, B., Perez-Perez, G. I., Blaser, M. J., and Sherman, P. M. (1990) N. Engl. J. Med. 322, 359-363. 76. Shomer, N. H., Dangler, C. A., Whary, M. T., and Fox, J. G. (1998) Infect. Immun. 66, 2614-2618. 77. Nakajima, S., Krishnan, B., Ota, H., Segura, A. M., Hattori, T., Graham, D. Y., and Genta, R. M. (1997) Gastroenterology 113, 746-754. 78. De Bolos, C., Garrido, M., and Real, F. X. (1995) Gastroenterology 109, 723-734. 79. Murata, K., Egami, H., Shibata, Y., Sakamoto, K., Misumi, A., and Ogawa, M. (1992) Am. J. Clin. Pathol. 98, 67-75. 80. Boldyrev, I. A., Zhai, X., Momsen, M. M., Brockman, H. L., Brown, R. E., and Molotkovsky, J. G. (2007) J. Lipid Res. 48, 1518-1532. 81. Landrum, M., Smertenko, A., Edwards, R., Hussey, P. J., and Steel, P. G. (2010) Plant J. 62, 529-538. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/44120 | - |
| dc.description.abstract | 諸多疾病伴隨岩藻醣水解酶活性異常，先前研究發現感染幽門螺旋桿菌會促使人類癌症細胞分泌第二型岩藻醣水解酶 (FUCA2) 至胞外，造成致病菌攝取宿主之岩藻醣。但第二型岩藻醣水解酶在感染過程中扮演之角色與生化性質目前仍不清楚。
本研究為了解第二型岩藻醣水解酶的生化性質及其在幽門螺旋桿菌感染時扮演之角色，首先在人類 293T 細胞表現出有活性的第二型岩藻醣水解酶，檢測其 pH 值對活性之影響 (pH profile)、酵素動力學常數、基質專一性等性質，並與第一型岩藻醣水解酶 (FUCA1) 作比較。這二個酵素的 pH profile 同樣有二個最佳活性區，分別在 pH 4.5 及 pH 6.5。接著用七種含螢光的醣分子檢測基質專一性，發現第一型岩藻醣水解酶可水解 Fuc α-1,2 Gal β-1,4、core Fuc α-1,6 biantennary、Lea、H type I 四種基質上的岩藻醣，而第二型岩藻醣水解酶則水解 Fuc α-1,2 Gal β-1,4、core Fuc α-1,6 biantennary 二種基質的岩藻醣。後者與由人類癌症細胞與幽門螺旋桿菌共同培養液中取得的酵素混合物，作出的結果一致。 另外在幽門螺旋桿菌感染前後的岩藻醣水解酶變化的研究中，利用含螢光基團的岩藻醣水解酶活性分子探針 (ABP) 偵測，發現致病菌感染不僅使胞外培養液中岩藻醣水解酶量及活性上升，也使胞內岩藻醣水解酶分布及活性增加。 綜合以上研究，推測幽門螺旋桿菌感染是會使細胞內岩藻醣水解酶表現增加，進而分泌至胞外。而第二型岩藻醣水解酶在感染時會幫助幽門螺旋桿菌獲取宿主細胞的岩藻醣，且不破壞幽門螺旋桿菌附著與偽裝宿主之醣結構。 | zh_TW |
| dc.description.abstract | Abnormal fucosidase activity has been associated with many diseases, such as hepatocellular cancer and breast cancer. Previous study indicated that FUCA2 is released by gastric epithelial cells upon Helicobacter pylori infection, and thus a potential diagnostic marker for gastric diseases. Although the enzyme is found to be critical to the bacterial growth, infection, adhesion and pathogenecity, its role and physiological function still remain ambiguous.
In this thesis study, the stable lines of recombinant FUCA2 were constructed to determine the biochemical features of FUCA2 such as pH profile, kinetic parameters and substrate specificity. The results indicated that FUCA2 is optimally active at pH 4.5 and pH 6.5, similar to FUCA1. Via the use of seven pyridylamino-conjugated sugar substrates, the HPLC analysis indicates that FUCA1 preferentially hydrolyzes L-fucose from H type II, Lewis antigen A, H type I, and core fucose in the biantennary, while FUCA2 will catalyze the hydrolysis of the first and the last substrates only. Additionally, the fluorescent, activity-based probes LCL10002 and LCL10003, prepared by Prof. L. C. Lo and coworkers in NTU, were studied to detect α-L-fucosidase activity in the dot blot analysis and fluorescent microscopy. The results indicated that LCL10003 is better than LCL10002 in terms of sensitivity, and that both secreted and intracellular fucosidase activities increase after H. pylori infection. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-15T02:40:50Z (GMT). No. of bitstreams: 1 ntu-100-R98b46010-1.pdf: 5538368 bytes, checksum: 63f4895e6b736e34a8ffaf85d422aced (MD5) Previous issue date: 2011 | en |
| dc.description.tableofcontents | 縮寫表 ………………………………………………………………………………. I
中文摘要 …………………………………………………………………………… III 英文摘要 ………………………………………………………………………….... IV 圖目錄 …………………………………………………………………………….... IX 表目錄 ……………………………………………………………………………... XII 1. 緒論 1.1 岩藻醣水解酶概述 ……………………………………………………….. 01 1.2 人類岩藻醣水解酶之功能與相關疾病 ………………………………….. 03 1.3 路易士抗原之角色 ……………………………………………………….. 06 1.4 醣苷水解酶之偵測 ……………………………………………………….. 08 1.5 研究目標 ………………………………………………………………….. 13 2. 結果 2.1 人類第二型岩藻醣水解酶重組蛋白之建構與生化特性 2.1.1 質體建構與純化檢定 ………………………………………………... 15 2.1.2 活性檢測 ……………………………………………………………... 15 2.1.3 pH 活性分布圖及 Km、kcat 值 ……………………………………. 16 2.1.4 基質專一性測定 ……………………………………………………... 17 2.2 感染胃幽門螺旋桿菌而影響之岩藻醣相關結構 2.2.1 共同培養液水解之岩藻醣相關結構 ………………………………... 18 2.2.2 幽門螺旋桿菌促使細胞分泌的岩藻醣水解酶基質專一性 ………... 18 2.3 酵素活性分子探針 (Activity-based probe, ABP) 之偵測 2.3.1 LCL10003 分子探針結構與作用機制 …………………………….. 20 2.3.2 活性分子探針之性質與偵測極限（以 Thermotoga maritime 岩藻醣水解酶為模式研究） …………………………………………………… 20 2.3.3 人類第二型岩藻醣水解酶重組蛋白之偵測 ………………………... 22 2.3.4 人類癌症細胞與胃幽門螺旋桿菌共同培養液之偵測 ……………... 22 2.3.5 活性分子探針之細胞成像螢光觀測 ………………………………... 23 3. 討論 3.1 人類第一型及第二型岩藻醣水解酶 pH 活性分布異同比較 …………. 26 3.2 人類第一型及第二型岩藻醣水解酶基質專一性探討 ………………….. 26 3.3 單一 Tm FA 及 Tm FA-BSA 在不同 pH 值被 LCL10003 標記之結果 28 3.4 共同培養液中岩藻醣水解酶之 PAGE 、 dot blotting 、 assay 偵測 …. 29 3.5 LCL10003 應用於細胞成像之結果探討 ………………………………... 29 4. 結論 …………………………………………………………………………….. 31 5. 材料與方法 5.1 人類第二型岩藻醣水解酶重組蛋白之表現與純化 …………………….. 32 5.2 岩藻醣水解酶之活性測定 …………………….…………………………. 33 5.3 岩藻醣水解酶之酵素動力分析 ………………………………………….. 33 5.4 高效能液相層析法分析及氨基吡啶醣 ………………………………….. 34 5.5 菌株與細胞株 …………………………………….………………………. 35 5.6 幽門螺旋桿菌與人類癌症細胞共同培養條件 ………………………….. 35 5.7 次細胞蛋白質抽取 ……………………………………………………….. 36 5.8 凝集素微陣列分析 ……………………………………………………….. 36 5.9 Thermotoga maritima岩藻醣水解酶及人類第一型岩藻醣水解酶重組蛋白之表現與純化 ……………………………………………………………….. 36 5.10 活性分子探針反應條件 ………………………………………………….. 37 5.11 共同培養液之.dot blotting 反應條件 ……………………………………. 38 5.12 共同培養液之活性分析反應條件 ……………………………………….. 38 5.13 共軛焦螢光顯微鏡與細胞成像 ……………………………………….. 39 6. 參考文獻 ……………………………………………………………………….. 40 7. 圖表 …………………………………………………………………………….. 53 | |
| dc.language.iso | zh-TW | |
| dc.title | 人類第二型岩藻醣水解酶之生化性質探討及偵測 | zh_TW |
| dc.title | Characterization and Detection of Human α-L-Fucosidase 2 | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 99-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 邱繼輝(Kay-Hooi Khoo),羅禮強(Lee-Chiang Lo) | |
| dc.subject.keyword | 人類第二型岩藻醣水解酶,幽門螺旋桿菌,基質專一性,活性分子探針, | zh_TW |
| dc.subject.keyword | α-L-fucosidase,H. pylori,substrate specificity,avtivity-based probe, | en |
| dc.relation.page | 79 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2011-08-17 | |
| dc.contributor.author-college | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 生化科學研究所 | zh_TW |
| 顯示於系所單位： | 生化科學研究所 | |
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