幾丁聚醣/聚己內酯之混掺基材對於調控間葉幹細胞軟骨分化之研究

Abstract

細胞型態和形狀會因外界的環境不同而影響細胞的基本功能，例如：分化及增生，利用基材(substrate)來影響細胞的貼附行為，改變細胞型態和形狀，將可控制細胞的生理功能。通過美國食品藥物管理局(FDA)認證，幾丁聚醣(chitosan) 及聚己內酯(PCL)皆為可裂解的生醫材料，其中，幾丁聚醣被廣泛用於傷口敷料及軟骨組織工程，但也有些研究指出幾丁聚醣不利於纖維母細胞的貼附及增生。經研究證明，聚己內酯有助於纖維母細胞的貼附及生長，故被廣泛用於手術縫線。我們期望建立骨髓間葉幹細胞(bone marrow mesenchymal stem cells)培養於不同比例的混摻材料進而調控間葉幹細胞的貼附能力、增生並軟骨分化的可行性。因此，本研究將混和不同比例的聚己內酯及幾丁聚醣，藉以創造出新的混摻材料，透過此混摻材料來調控細胞的貼附能力，進一步達到以不同的細胞型態和形狀來調控幹細胞(stem cells)對於軟骨分化的功能。

In different environment, the cellular morphology and shape will affect its physiological function, such as differentiation and proliferation. Therefore, it is a niche to control the physiological function of cell using cultural substrate to regulate the cellular attachment which could induce morphology and shape. Two biodegraded materials (e.g. chitosan and PCL), which were approved by Food and Drug Administration (FDA, US) were used in this study. Chitosan is wildly employed as artificial skin and cartilage tissue engineering. However, some studies indicated that chitosan was not a suitable material for fibroblast attachment and proliferation. Another material, PCL is also wildly used in suture and lots of studies demonstrated that the PCL could promote fibroblast attachment and growth. We would like establish a model of ability of cell adhesion, proliferation rate, and possibility of MSCs differentiate into chondrocyte when cultured on PCL and chitosan blended substrates. In this study, we try to mix chitosan and PCL which could then modulate the adhesive on ability of cells. Further, to promote the function of chondrogenic differentiation of stem cells by altering cell morphology and cell shape.