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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 嚴震東(Cheng-Tung Yen) | |
dc.contributor.author | Yi-Fang Liao | en |
dc.contributor.author | 廖儀芳 | zh_TW |
dc.date.accessioned | 2021-06-15T02:31:42Z | - |
dc.date.available | 2016-08-22 | |
dc.date.copyright | 2011-08-22 | |
dc.date.issued | 2011 | |
dc.date.submitted | 2011-08-17 | |
dc.identifier.citation | Anderson, M.P., Mochizuki, T., Xie, J., Fischler, W., Manger, J.P., Talley, E.M., Scammell, T.E., Tonegawa, S., 2005. Thalamic Cav3.1 T-type Ca2+ channel plays a crucial role in stabilizing sleep. Proc Natl Acad Sci U S A. 102, 1743-8.
Avoli, M., Gloor, P., Kostopoulos, G., Gotman, J., 1983. An analysis of penicillin-induced generalized spike and wave discharges using simultaneous recordings of cortical and thalamic single neurons. J Neurosci. 50, 819-37. Barbaresi, P., Spreafico, R., Frassoni, C., Rustioni, A., 1986. GABAergic neurons are present in the dorsal column nuclei but not in the ventroposterior complex of rats. Brain res. 382, 305-26. Bourinet, E., Alloui, A., Monteil, A., Barrere, C., Couette, B., Poirot, O., Pages, A., McRory, J., Snutch, T.P., Eschalier, A., Nargeot, J., 2005. Silencing of the Cav3.2 T-type calcium channel gene in sensory neurons demonstrates its major role in nociception. EMBO J. 24, 315-24. Carbone, E., Lux, H.D., 1984. A low voltage-activated, fully inactivating Ca channel in vertebrate sensory neurones. Nature. 310, 501-2. Carbone, E., Lux, H.D., 1987. Kinetics and selectivity of a low-voltage-activated calcium current in chick and rat sensory neurones. J Physiol. 386, 547-70. Chen, W.K., Liu, I.Y., Chang, Y.T., Chen, Y.C., Chen, C.C., Yen, C.T., Shin, H.S., 2010. Ca(v)3.2 T-type Ca2+ channel-dependent activation of ERK in paraventricular thalamus modulates acid-induced chronic muscle pain. J Neurosci. 30, 10360-8. Chevalier, M., Lory, P., Mironneau, C., Macrez, N., Quignard, J.F., 2006. T-type CaV3.3 calcium channels produce spontaneous low-threshold action potentials and intracellular calcium oscillations. Eur J Neurosci. 23, 2321-9. Choi, S., Na, H.S., Kim, J., Lee, J., Lee, S., Kim, D., Park, J., Chen, C.C., Campbell, K.P., Shin, H.S., 2007. Attenuated pain responses in mice lacking Ca(V)3.2 T-type channels. Genes Brain Behav. 6, 425-31. Contreras, D., Steriade, M., 1996. Spindle oscillation in cats: the role of corticothalamic feedback in a thalamically generated rhythm. J Physiol. 490, 159-79. Coulter, D.A., Huguenard, J.R., Prince, D.A., 1989. Calcium currents in rat thalamocortical relay neurones: kinetic properties of the transient, low-threshold current. J Physiol. 414, 587-604. Danober, L., Deransart, C., Depaulis, A., Vergnes, M., Marescaux, C., 1998. Pathophysiological mechanisms of genetic absence epilepsy in the rat. Prog Neurobiol. 55, 27-57. De Luca, M., Beckmann, C.F., De Stefano, N., Matthews, P.M., Smith, S.M., 2006. fMRI resting state networks define distinct modes of long-distance interactions in the human brain. Neuroimage. 29, 1359-67. Deschenes, M., Paradis, M., Roy, J.P., Steriade, M., 1984. Electrophysiology of neurons of lateral thalamic nuclei in cat: resting properties and burst discharges. J Neurosci. 51, 1196-219. Deschenes, M.S.a.M., 1984. The thalamus as a neuronal oscillator. Brain Res Rev. 320, 1-63. Domich, L., Oakson, G., Steriade, M., 1986. Thalamic Burst Patterns in the Naturally Sleeping Cat - a Comparison between Cortically Projecting and Reticularis Neurons. J Physiol. 379, 429-449. Guido, W., Lu, S.M., Sherman, S.M., 1992. Relative contributions of burst and tonic responses to the receptive field properties of lateral geniculate neurons in the cat. J Neurophysiol. 68, 2199-211. Harris, R.M., 1986. Morphology of physiologically identified thalamocortical relay neurons in the rat ventrobasal thalamus. J Comp Neurol. 251, 491-505. Harris, R.M., Hendrickson, A.E., 1987. Local circuit neurons in the rat ventrobasal thalamus--a GABA immunocytochemical study. Neuroscience. 21, 229-36. Hirayama, T., Dostrovsky, J.O., Gorecki, J., Tasker, R.R., Lenz, F.A., 1989. Recordings of abnormal activity in patients with deafferentation and central pain. Stereotact Funct Neurosurg. 52, 120-6. Hughes, S.W., Cope, D.W., Blethyn, K.L., Crunelli, V., 2002. Cellular mechanisms of the slow (<1 Hz) oscillation in thalamocortical neurons in vitro. Neuron. 33, 947-58. Hughes, S.W., Lorincz, M., Cope, D.W., Blethyn, K.L., Kekesi, K.A., Parri, H.R., Juhasz, G., Crunelli, V., 2004. Synchronized oscillations at alpha and theta frequencies in the lateral geniculate nucleus. Neuron. 42, 253-68. Hughes, S.W., Errington, A., Lorincz, M.L., Kekesi, K.A., Juhasz, G., Orban, G., Cope, D.W., Crunelli, V., 2008. Novel modes of rhythmic burst firing at cognitively-relevant frequencies in thalamocortical neurons. Brain Res. 1235, 12-20. Huguenard, J.R., 1996. Low-threshold calcium currents in central nervous system neurons. Annu Rev Physiol. 58, 329-48. Jeanmonod, D., Magnin, M., Morel, A., 1996. Low-threshold calcium spike bursts in the human thalamus. Common physiopathology for sensory, motor and limbic positive symptoms. Brain. 119 363-75. Jia, F., Pignataro, L., Schofield, C.M., Yue, M., Harrison, N.L., Goldstein, P.A., 2005. An extrasynaptic GABAA receptor mediates tonic inhibition in thalamic VB neurons. J Neurophysiol. 94, 4491-501. Joksovic, P.M., Bayliss, D.A., Todorovic, S.M., 2005. Different kinetic properties of two T-type Ca2+ currents of rat reticular thalamic neurones and their modulation by enflurane. J Physiol. 566, 125-42. Jones, E.G., Burton, H., 1974. Cytoarchitecture and somatic sensory connectivity of thalamic nuclei other than the ventrobasal complex in the cat. J Comp Neurol. 154, 395-432. Jones, E.G., Friedman, D.P., Hendry, S.H., 1982. Thalamic basis of place- and modality-specific columns in monkey somatosensory cortex: a correlative anatomical and physiological study. J Neurophysiol. 48, 545-68. Jones, E.G., 2007. The Thalamus, Vol., Cambridge University Press, New York. Khosravani, H., Altier, C., Simms, B., Hamming, K.S., Snutch, T.P., Mezeyova, J., McRory, J.E., Zamponi, G.W., 2004. Gating effects of mutations in the Cav3.2 T-type calcium channel associated with childhood absence epilepsy. J Biol Chem. 279, 9681-4. Kim, D., Song, I., Keum, S., Lee, T., Jeong, M.J., Kim, S.S., McEnery, M.W., Shin, H.S., 2001. Lack of the burst firing of thalamocortical relay neurons and resistance to absence seizures in mice lacking alpha(1G) T-type Ca(2+) channels. Neuron. 31, 35-45. Kim, D., Park, D., Choi, S., Lee, S., Sun, M., Kim, C., Shin, H.S., 2003. Thalamic control of visceral nociception mediated by T-type Ca2+ channels. Science. 302, 117-9. Kobayashi, K., Winberry, J., Liu, C.C., Treede, R.D., Lenz, F.A., 2009. A painful cutaneous laser stimulus evokes responses from single neurons in the human thalamic principal somatic sensory nucleus ventral caudal (Vc). J Neurophysiol. 101, 2210-7. Kozlov, A.S., McKenna, F., Lee, J.H., Cribbs, L.L., Perez-Reyes, E., Feltz, A., Lambert, R.C., 1999. Distinct kinetics of cloned T-type Ca2 + channels lead to differential Ca2 + entry and frequency-dependence during mock action potentials. Eur J Neurosci. 11, 4149-58. Lee, J., Kim, D., Shin, H.S., 2004. Lack of delta waves and sleep disturbances during non-rapid eye movement sleep in mice lacking alpha1G-subunit of T-type calcium channels. Proc Natl Acad Sci U S A. 101, 18195-9. Lee, J.H., Daud, A.N., Cribbs, L.L., Lacerda, A.E., Pereverzev, A., Klockner, U., Schneider, T., Perez-Reyes, E., 1999. Cloning and expression of a novel member of the low voltage-activated T-type calcium channel family. J Neurosci. 19, 1912-1921. Lee, J.I., Ohara, S., Dougherty, P.M., Lenz, F.A., 2005. Pain and temperature encoding in the human thalamic somatic sensory nucleus (Ventral caudal): inhibition-related bursting evoked by somatic stimuli. J Neurophysiol. 94, 1676-87. Lenz, F.A., Kwan, H.C., Dostrovsky, J.O., Tasker, R.R., 1989. Characteristics of the bursting pattern of action potentials that occurs in the thalamus of patients with central pain. Brain Res. 496, 357-60. Llinas, R., Yarom, Y., 1981. Properties and distribution of ionic conductances generating electroresponsiveness of mammalian inferior olivary neurones in vitro. J Physiol. 315, 569-84. Lorincz, M.L., Crunelli, V., Hughes, S.W., 2008. Cellular dynamics of cholinergically induced alpha (8-13 Hz) rhythms in sensory thalamic nuclei in vitro. J Neurosci. 28, 660-71. Lorincz, M.L., Geall, F., Bao, Y., Crunelli, V., Hughes, S.W., 2009a. ATP-dependent infra-slow (<0.1 Hz) oscillations in thalamic networks. PLoS One. 4, e4447. Lorincz, M.L., Kekesi, K.A., Juhasz, G., Crunelli, V., Hughes, S.W., 2009b. Temporal framing of thalamic relay-mode firing by phasic inhibition during the alpha rhythm. Neuron. 63, 683-96. Lu, S.M., Guido, W., Sherman, S.M., 1992. Effects of membrane voltage on receptive field properties of lateral geniculate neurons in the cat: contributions of the low-threshold Ca2+ conductance. J Neurophysiol. 68, 2185-98. McCarley, R.W., Benoit, O., Barrionuevo, G., 1983. Lateral geniculate nucleus unitary discharge in sleep and waking: state- and rate-specific aspects. J Neurophysiol. 50, 798-818. Ohara, S., Taghva, A., Kim, J.H., Lenz, F.A., 2007. Spontaneous low threshold spike bursting in awake humans is different in different lateral thalamic nuclei. Exp Brain Res. 180, 281-8. Perez-Reyes, E., Cribbs, L.L., Daud, A., Lacerda, A.E., Barclay, J., Williamson, M.P., Fox, M., Rees, M., Lee, J.H., 1998. Molecular characterization of a neuronal low-voltage-activated T-type calcium channel. Nature. 391, 896-900. Peschanski, M., Guilbaud, G., Gautron, M., 1980. Neuronal responses to cutaneous electrical and noxious mechanical stimuli in the nucleus reticularis thalami of the rat. Neurosci Lett. 20, 165-70. Radhakrishnan, V., Tsoukatos, J., Davis, K.D., Tasker, R.R., Lozano, A.M., Dostrovsky, J.O., 1999. A comparison of the burst activity of lateral thalamic neurons in chronic pain and non-pain patients. Pain. 80, 567-75. Sherman, S.M., Guillery, R.W., 2006. exploring the thalamus and its role in cortical function, Vol., The MIT Press, London. Shin, H.S., Cheong, E.J., Choi, S., Lee, J., Na, H.S., 2008. T-type Ca2+ channels as therapeutic targets in the nervous system. Curr Opin Pharmacol. 8, 33-41. Steriade, M., Domich, L., Oakson, G., 1986. Reticularis thalami neurons revisited: activity changes during shifts in states of vigilance. J Neurosci. 6, 68-81. Steriade, M., Amzica, F., 1994. Dynamic coupling among neocortical neurons during evoked and spontaneous spike-wave seizure activity. J Neurosci. 72, 2051-69. Talley, E.M., Cribbs, L.L., Lee, J.H., Daud, A., Perez-Reyes, E., Bayliss, D.A., 1999. Differential distribution of three members of a gene family encoding low voltage-activated (T-type) calcium channels. J Neurosci. 19, 1895-911. Todorovic, S.M., Meyenburg, A., Jevtovic-Todorovic, V., 2002. Mechanical and thermal antinociception in rats following systemic administration of mibefradil, a T-type calcium channel blocker. Brain Res. 951, 336-40. Tsoukatos, J., Kiss, Z.H., Davis, K.D., Tasker, R.R., Dostrovsky, J.O., 1997. Patterns of neuronal firing in the human lateral thalamus during sleep and wakefulness. Exp Brain Res. 113, 273-82. Ulrich, D., Huguenard, J.R., 1996. Gamma-aminobutyric acid type B receptor-dependent burst-firing in thalamic neurons: a dynamic clamp study. Proc Natl Acad Sci U S A. 93, 13245-9. von Krosigk, M., Bal, T., McCormick, D.A., 1993. Cellular mechanisms of a synchronized oscillation in the thalamus. Science. 261, 361-4. Yen, C.T., Conley, M., Hendry, S.H., Jones, E.G., 1985. The morphology of physiologically identified GABAergic neurons in the somatic sensory part of the thalamic reticular nucleus in the cat. J Neurosci. 5, 2254-68. Yen, C.T., Shaw, F.Z., 2003. Reticular thalamic responses to nociceptive inputs in anesthetized rats. Brain Res. 968, 179-91. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/43887 | - |
dc.description.abstract | T-type Ca2+ channel (T-channel)是引起視丘神經元bursting放電的關鍵離子通道。其亞型Cav3.1在視丘腹尾核區ventral posterior thalamic nucleus (VP),及Cav3.2和Cav3.3在視丘網狀核區(Reticular thalamic nucleus, RT)的表現並具有組織專一性的特徵。近來研究發現在疼痛刺激下的行為反應在Cav3.1基因缺失( knock out, KO)小鼠有增加情形,而在Cav3.2 KO則是減少。VP是體感覺訊息傳遞上重要的傳遞者,而與其密集相互投射的抑制性RT神經元均利用bursting做為主要的放電模式之一。因此,在實驗中同時記錄此二核區的神經元放電可幫助了解T-channel變異所造成的神經元bursting的變化。
利用多通道單一神經元胞外記錄方式,我們同步記錄麻醉下Cav3.1和Cav3.2 KO小鼠VP和RT的神經元活性。在第一部分的結果主要是在比較此兩種T-channel KO小鼠VP和RT神經元在自發性活性的差異。我們發現,在VP核區中僅有1%體感覺神經元與痛覺相關;在RT則多達50%,同時這個比例在Cav3.2 KO明顯減少。Bursting在Cav3.1VP幾乎消失的結果是與前人一致的。在Cav3.2 KO則是看到不僅RT bursting減少並且bursting強度明顯衰弱,同時還發現Cav3.2 KO的VP burst活性增加。 目前很少研究探討視丘對傷害與非傷害性刺激時反應的放電模式特別是反應模式與爆裂式放電的關係。因此,本論文第二部分利用Cav3.1 和Cav3.2這兩種在VP和RT具有專一性表現的小鼠觀察burst firing受影響後,對刺激的反應的變化。我們發現在正常小鼠中,非傷害性刺激在視丘VP和RT引起以burst firing 為主的反應形式,而傷害性刺激則引起單一動作電位的反應。此現象在T-channel KO小鼠也可觀察到,但在Cav3.1 KO VP對非傷害性的刺激,burst firing的比例顯著低於正常小鼠而其RT以單一動作電位反應傷害性刺激的表現更顯著。在Cav3.2 KO,VP 和 RT burst firing對非傷害性物的物理性刺激的反應則是減少。Cav3.2 KO burst firing在由雷射熱痛引發的反應中,表現較少burst firing且強度也較低。另外,Cav3.2 VP和RT對雷射和電刺激的反應延遲時間都較正常鼠長。 本研究最後一部分比較了視丘內部神經元在較長時間的交互活動。我們發現視丘神經元具有一個共同漲落的慢周期變化。而這個週期性變化在VP和RT中呈現此消彼長相反的活性。這個震盪中神經元間彼此的關連性在Cav3.1和Cav3.2都較為減弱。 綜合以上結果,我們認為T-channel參與了視丘VP和RT的節律性活性。而此視丘的節律性活性變化可能對T-channel缺失的小鼠在疼痛行為上的表現有貢獻。 | zh_TW |
dc.description.abstract | Low-threshold T-type calcium channels (T-channels) are critically important in the generation of thalamic burst activities. Different subtypes of T-channel distributed differentially in thalamic ventroposterior nucleus (VP, Cav3.1) and reticular thalamic nucleus (RT, Cav3.2 and Cav3.3). Furthermore, T-channel knockout (KO) mice showed contrast nociceptive behaviors, such that an increase was seen in Cav3.1 KO and a decrease in Cav3.2 KO. Considering the important role in sensory relay function of VP neuron and it’s densely reciprocal connection to GABAergic RT, it is important to examine VP and RT neurons simultaneous and compare their bursting activity in the T-channel KO mice.
We used multi-channel single-unit recording method to study the changes in the firing property of VP and RT neurons in Cav3.1 and 3.2 KO mice in the anesthetized condition. In the first part of this study, we compared the spontaneous burst and tonic firing activities of VP and RT neurons among the two KO and their wild-type (WT) control mice. We found that in WT mice, 50% somatosensory RT neurons and less than 1% VP neurons were nociceptive. Percentages of nociceptive neurons decreased in the RT and VP in the Cav3.2 KO mice. Cav3.1 KO lost most burst firing in VP. In Cav3.2 KO, we found fewer and weakened RT burst firing and tonic RT firing lost their regularity. Interestingly, Cav3.2 KO also showed increased and stronger VP burst firing. In the second part of the present study, the responsiveness of the VP and RT burst firing to tactile and noxious stimuli in the Cav3.1 and Cav3.2 KO were studied. We found that the innocuous stimulation induced response firing in VP and RT neurons were mostly burst firing, whereas noxious stimulation induced mostly single spikes. T-channel KO mice showed similar response pattern. Cav3.1 KO particularly showed less proportion of VP burst firing in response to light touch stimulation and RT neurons showed more prominently single spike firings in response to pinch stimulation compare to their WT control mice. In Cav3.2 KO, VP and RT neurons showed reduced bursts of mechanical innocuous stimulation evoked response. In laser heat stimulation evoked responses, Cav3.2 KO RT neurons showed reduced burst firing response in both percentage and amplitude. Furthermore, Cav3.2 KO VP and RT neurons had longer response onset latencies in 1.5 T electrical stimulation and laser heat stimulation. In the third part of this study, we analyzed thalamic neuron activities in a longer time scale to see whether T channel deficits interfered with intra thalamic interactions. A slow reciprocal VP and RT oscillations was found. This oscillation was modality independent, but it was weakened in Cav3.1 and Cav3.2 KO mice. The results showed that T-channel is important in thalamic burst rhythm, slow rhythm, and thalamocortical neuron and reticular neuron interaction. These factors may contribute to rodent pain function. In conclusion, T-channels are involved in thalamic VP and RT rhythmic activities. The changes in thalamic rhythmicity might contribute to the changed nocifensive behaviors in the T channel defective mice. | en |
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dc.description.tableofcontents | CONTENTS
口試委員會審定書 I 誌謝 II CONTENTS III LIST OF TABLES AND FIGURES V 中文摘要 IX ABSTRACT XI INTRODUCTION 1 THALAMUS SENSORY PATHWAY VP 2 THALAMUS SENSORY PATHWAY RT 4 THALAMIC BURST AND TONIC ACTIVITIES IN BEHAVIORAL STATE 5 THALAMIC ACTIVITY IN DISEASE 6 T CHANNEL, BURST AND NOCICEPTION 7 OTHER THALAMIC RHYTHMS 10 MOTIVATION AND SPECIFIC AIMS 11 MATERIALS AND METHODS 12 ANIMAL PREPARATION 12 EXTRACELLULAR RECORDING 13 STIMULATIONS 14 ANALYSIS 16 HISTOLOGY 18 STATISTICS 19 RESULTS 20 COMPARISON OF VP AND RT SPONTANEOUS FIRING PROPERTIES IN CAV3.1, CAV3.2 KO AND WT MICE 20 INFLUENCE BY DIFFERENT ANESTHETICS 27 COMPARISON OF VP AND RT RESPONSIVENESS TO INNOCUOUS AND NOXIOUS STIMULATIONS IN THE 3 MICE STRAINS 29 THALAMIC NEURONS INTERACTION 37 DISCUSSION 40 T CHANNEL AND THALAMIC BURST FIRING PATTERN 41 T CHANNEL AND OTHER THALAMIC RHYTHMS 44 T CHANNEL AND NOCICEPTION 46 REFERENCES 48 List of Tables and Figures TABLE 1. BURSTING NEURONS WERE HARDER TO FIND IN CAV3.2 KO RT; NOCICEPTIVE RELATED NEURONS WERE OF LOWER PERCENTAGE IN RT AND VP OF CAV3.2 KO MICE 53 TABLE 2. LIGHT TOUCH AND PINCH EVOKED BURST FIRING FREQUENCY REDUCED IN CAV3.1 KO MICE 54 TABLE 3. VP AND RT CELLS SHOW REDUCED BURSTS ON LASER EVOKED A AND C COMPONENTS IN CAV3.2 KO 55 FIGURE1. IDENTIFICATION OF RECORDING SITES. 56 FIGURE 2. EXAMPLES OF SPONTANEOUS FIRING ACTIVITY OF VP UNITS IN A WT (A, B, AND C), A CAV3.1 KO (D, E, AND F) AND A CAV3.2 KO (G, H AND I) MICE. 57 FIGURE 3. EXAMPLES OF SPONTANEOUS BURST AND TONIC ACTIVITY OF RT UNITS IN A WT (A, B, AND C), A CAV3.1 KO (D, E AND F) AND A CAV3.2 KO (G, H AND I) MICE. 58 FIGURE 4. COMPARISON OF ACCELERANDO-DECELERANDO FIRING PATTERN OF RT NEURONS IN WT, CAV3.1 AND CAV3.2 KO MICE. SIGNIFICANCE DIFFERENCES WERE ONLY FOUND FOR RT UNITS IN CAV3.2 KO MICE. 59 FIGURE 5. COMPARISON OF BURST FIRING PROPERTIES OF RT AND VP UNITS IN WT, CAV3.1 AND CAV3.2 KO MICE. 60 FIGURE 6. THE VP AND RT BURST FIRING CHANGES IN CAV3.1 KO MICE WERE NOT MODALITY DEPENDENT. 61 FIGURE 7. THE VP AND RT BURST FIRING CHANGES IN CAV3.2 KO MICE WERE NOT MODALITY DEPENDENT. 62 FIGURE 8. THE GRADED ELONGATED INTER-SPIKE-INTERVAL OF LONG LASTING TONIC FIRING IN CAV3.1 FITTED LINEAR REGRESSION LINE AS WELL AS WT CONTROL. 63 FIGURE 9. CAV3.2 KO MICE LOSS THEIR REGULARITY IN RT LONG LASTING TONIC FIRING. 64 FIGURE 10. EXAMPLE OF THE RESPONSES OF LT, WDR AND NOCICEPTIVE RELATED VP NEURONS IN WT MICE. 65 FIGURE 11. EXAMPLE OF THE RESPONSES OF LT, WDR AND NOCICEPTIVE RELATED VP NEURONS IN CAV3.1 MICE. 66 FIGURE 12. EXAMPLE OF THE RESPONSES OF LT AND WDR VP NEURONS IN CAV3.2 MICE. 67 FIGURE 13. EXAMPLE OF THE RESPONSES OF PINCH EXCITE AND PINCH DELAY_EXCITE RT UNITS IN WT MICE. 68 FIGURE 14. EXAMPLES OF THE RESPONSES OF PINCH EXCITE AND PINCH INHIBIT _EXCITE RT UNITS IN CAV3.1 KO MICE. 69 FIGURE 15. EXAMPLE OF THE RESPONSES OF PINCH EXCITE AND PINCH INHIBIT _EXCITE RT UNITS IN CAV3.2 KO MICE. 70 FIGURE 16. EXAMPLE OF VP NEURONS BURST FIRING RESPONSE TO LIGHT TOUCH AND PINCH IN A WT (A), A CAV3.1 KO (B) AND A CAV3.2 KO (C) MICE. 71 FIGURE 17. COMPARISON OF VP AND RT BURST AND TONIC FIRINGS IN RESPONSES TO LIGHT TOUCH STIMULATION IN CAV3.1, CAV3.2 KO AND WT CONTROL. 72 FIGURE 18. EXAMPLE OF RT NEURONS BURST FIRING RESPONSE CHANGES OF LIGHT TOUCH AND PINCH IN A WT (A), A CAV3.1 KO (B) AND A CAV3.2 KO (C) MICE. 73 FIGURE 19. THE COMPARISON OF WT, CAV3.1 AND CAV3.2 KO VP AND RT BURST AND TONIC FIRINGS IN RESPONSE TO PINCH STIMULATION. 74 FIGURE 20. THE RT BURST FIRING REDUCED DURING PINCH AND AFTER PINCH STIMULATION IN 3 MICE STRAINS PARTICULARLY EXERTED IN CAV3.1 KO MICE. 75 FIGURE 21. THERE IS NO SIGNIFICANTLY DIFFERENT IN THE DELAY TIME IN RESPONSE TO PINCH STIMULATION OF RT NEURONS AMONG THE 3 MICE STRAINS. 76 FIGURE 22. EXAMPLE OF VP (A) AND RT (B) NEURONS RESPONSE TO 1.5T ELECTRICAL STIMULATION IN A WT MOUSE. 77 FIGURE 23. EXAMPLE OF VP (A) AND RT (B) NEURONS RESPONSE TO 1.5T ELECTRICAL STIMULATION IN A CAV3.2 KO MOUSE. 78 FIGURE 24. CAV3.2 KO RT NEURONS SHOWED DECREASED RESPONSIVENESS OF SPIKE FIRING (A) AND BURST FIRING (B) IN 1.5 T ELECTRICAL STIMULATION 79 FIGURE 25. CAV3.2 KO VP (A) AND RT (B) NEURONS SHOWED LONGER ONSET LATENCY OF 1.5T ELECTRICAL STIMULATION IN SPIKE AND BURST FIRINGS. 80 FIGURE 26. THERE IS NO DIFFERENCE IN RESPONSE MAGNITUDE OF SPIKE FIRING (A) AND BURST FIRING (B) BETWEEN CAV3.2 KO AND WT MICE 81 FIGURE 27. EXAMPLE OF VP (A) AND RT (B) NEURONS SPIKES AND BURSTS ACTIVITIES IN RESPONSE TO NOXIOUS LASER HEAT STIMULATIONS IN A WT MOUSE. 82 FIGURE 28. EXAMPLE OF VP (A) AND RT (B) NEURONS RESPONSE TO NOXIOUS LASER HEAT STIMULATION IN A CAV3.2 KO MOUSE. 83 FIGURE 29. THERE IS NO DIFFERENCE OF LASER HEAT EVOKED SPIKE FIRING (A) AND BURST FIRING (B) IN A (A) AND C (B) NOCICEPTIVE RELATED COMPONENTS BETWEEN CAV3.2 KO AND WT MICE. 84 FIGURE 30. CAV3.2 KO MICE SHOWED LONGER ONSET LATENCY IN LASER HEAT EVOKED SPIKE FIRING (A) AND BURST FIRING (B) RESPONSES. 85 FIGURE 31. THERE IS NO DIFFERENT IN LASER EVOKED VP SPIKE FIRING (A) AND BURST FIRING (B) IN NOCICEPTION RELATED A OR C COMPONENTS MAGNITUDE BETWEEN WT AND CAV3.2 KO MICE. 86 FIGURE 32. RT NEURONS SHOWED REDUCED SPIKE FIRING MAGNITUDE IN LASER HEAT INDUCED C COMPONENT IN CAV3.2 KO COMPARE TO WT MICE. 87 FIGURE 33. EXAMPLE OF THE SPONTANEOUS ACTIVITIES OF DIFFERENT SOMATOTOPIC RESPONSIVE VP AND RT UNITS. 88 FIGURE 34. THE DISTRIBUTION OF CORRELATION COEFFICIENT VALUE IN DIFFERENT COMPOSITION OF VP AND RT UNITS PAIRINGS. 90 FIGURE 35. CAV3.1 AND CAV3.2 KO SHOWED DECREASED CORRELATION IN THALAMIC VP AND RT NEURONS COMPARE TO WT MICE. 91 | |
dc.language.iso | en | |
dc.title | 瞬變型鈣離子通道對視丘腹側核和網狀核神經元放電模式與交互活動的影響 | zh_TW |
dc.title | Involvement of the T-type Calcium channel in discharge pattern and interaction of ventral posterior and reticular thalamic neurons | en |
dc.type | Thesis | |
dc.date.schoolyear | 99-2 | |
dc.description.degree | 博士 | |
dc.contributor.oralexamcommittee | 徐百川(Bai-Chuang Shyu),陳建璋(Chien-Chang Chen),陳志成(Chih-Cheng Chen),蔡孟利(Meng-Li Tsai),孫維仁(Wei-Zen Sun),溫永銳(Yuan-Jui Wen) | |
dc.subject.keyword | T通道, | zh_TW |
dc.subject.keyword | T-channel, | en |
dc.relation.page | 91 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2011-08-17 | |
dc.contributor.author-college | 生命科學院 | zh_TW |
dc.contributor.author-dept | 動物學研究所 | zh_TW |
顯示於系所單位: | 動物學研究所 |
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