請用此 Handle URI 來引用此文件:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/43726
完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 齊肖琪(Shau-Chi Chi) | |
dc.contributor.author | Ren-Xiang Wang | en |
dc.contributor.author | 王任翔 | zh_TW |
dc.date.accessioned | 2021-06-15T02:26:57Z | - |
dc.date.available | 2011-08-20 | |
dc.date.copyright | 2009-08-20 | |
dc.date.issued | 2009 | |
dc.date.submitted | 2009-08-17 | |
dc.identifier.citation | 鄭存明。2001。鱼類結病毒持續性感染之研究。國立台灣大學動物學研究所碩士論文。
Adachi, K., Ichinose, T., Takizawa, N., Watanabe, K., Kitazato, K. & Kobayashi, N. (2007). Inhibition of betanodavirus infection by inhibitors of endosomal acidification. Archives of Virology 152, 2217–2224. Arad, M., Seidman, C. E. & Seidman, J. G. (2007). AMP-activated protein kinase in the heart: role during health and disease. Circulation Research 100, 474-488. Arimoto, M., Mushiake, K. & Mizuta, Y. (1992). Detection of striped jack nervous necrosis virus (SJNNV) by enzyme-linked immunosorbent assay (ELISA). Fish Pathology 27, 191-195. Avi Sofer, K. L., Cory, M. J. & Leif W. E. (2005). Regulation of mTOR and cell growth in response to energy stress by REDD1. Molercular and Cellular Biology 25, 5834-5845. Azad IS, S. M., Thirunavukkarasu, A. R., Poornima, M., Kailasam, M., Rajan, J. J., Ali, S. A., Abraham, M. & Ravichandran, P. (2005). Nodavirus infection causes mortalities in hatchery produced larvae of Lates calcarifer: first report from India. Diseases of Aquatic Organisms 63, 113-118. Barker, D. E., MacKinnon, A. M., Boston, L., Burt, M. D., Cone, D. K., Speare, D. J., Griffiths, S., Cook, M., Ritchie, R. & Olivier, G. (2002). First report of piscine nodavirus infecting wild winter flounder Pleuronectes americanus in Passamaquoddy Bay, New Brunswick, Canada. Diseases of Aquatic Organisms 49, 99-105. Berkova, Z., Crawford, S. E., Trugnan, G., Yoshimori, T., Morris, A. P. & Estes, M. K. (2006). Rotavirus NSP4 induces a novel vesicular compartment regulated by calcium and associated with viroplasms. Jounal of Virology 80, 6061-6071. Bernales, S., McDonald, K. L. & Walter, P. (2006). Autophagy counterbalances endoplasmic reticulum expansion during the unfolded protein response. PLoS Biology 4, e423. Bienz, K., Egger, D. & Pasamontes, L. (1987). Association of polioviral proteins of the P2 genomic region with the viral replication complex and virus-induced membrane synthesis as visualized by electron microscopic immunocyto- chemistry and autoradiography. Virology 160, 220-226. Bloch, B., Gravningen, K. & Larsen, J. L. (1991). Encephalomyelitis among turbot associated with a picornavirus-like agent. Diseases of Aquatic Organisms 10, 65-70. Boya, P., González-Polo, R. A., Casares, N., Perfettini, J. L., Dessen, P., Larochette, N., Métivier, D., Meley, D., Souquere, S., Yoshimori, T., Pierron, G., Codogno, P. & Kroemer, G. (2005). Inhibition of macroautophagy triggers apoptosis. Molercular Cellullar Biology 25, 1025-1040. Byfield, M. P., Murray, J. T. & Backer, J. M. (2005). hVps34 is a nutrient- regulated lipid kinase required for activation of p70 S6 kinase. The Journal of Biological Chemistry 280, 33076-33082. Chan, S. W. & Egan, P. A. (2005). Hepatitis C virus envelope proteins regulate CHOP via induction of the unfolded protein response. The FASEB Journal 19, 1510-1512. Chen, L. J., Su, Y. C. & Hong, J. R. (2009). Betanodavirus non-structural protein B1: A novel anti-necrotic death factor that modulates cell death in early replication cycle in fish cells. Virology 385, 444-454. Chen, S. P., Wu, J. L., Su, Y. C. & Hong, J. R. (2007). Anti-Bcl-2 family members, zfBcl-x(L) and zfMcl-1a, prevent cytochrome c release from cells undergoing betanodavirus-induced secondary necrotic cell death. Apoptosis 12, 1043–1060. Chi, S. C., Hu, W. W. & Lo, B. J. (1999). Establishment and characterization of a continuous cell line( GF-1 ) derived from grouper, Epinephelus coioides ( Hamilton ) : a cell line susceptible to grouper nervous necrosis virus ( GNNV ). Journal of Fish Disease 22, 1-10. Chi, S. C., Lo, C. F., Kou, G. H., Chang, P. S., Peng, S. E. & Chen, S. N. (1997). Mass mortalities associated with viral nervous necrosis(VNN) disease in two species of hatchery-reared grouper, Epinephelus fuscogutatus and Epinephelus akaara ( Temminck and Schlegel ). Journal of Fish Disease 20, 185-193. Chi, S. C., Lo, B. J. & Lin, S. C. (2001). Characterization of grouper nervous necrosis virus(GNNV). Journal of Fish Disease 24, 3-13. Chi, S. C., Lo, B. J., Lin, S. C., Wen, W. W., Lo, C. F., Kou, G. H., & Chen, S. N. (2000). The survey of viral nervous necrosis among cultured groupers in Taiwan. In APEC FWG02/2000, 18-20. Chi, S. C., Shieh, J. R. & Lin, S. J. (2003). Genetic and antigenic analysis of betanodaviruses isolated from aquatic organisms in Taiwan. Disease of Aquatic Organisms 55, 221-228. Chi, S. C., Wu, Y. C. & Cheng, T. M. (2005). Persistent infection of betanodavirus in a novel cell line derived from the brain tissue of barramundi Lates calcarifer. Disease of Aquatic Organisms 65, 91-98. Chiang, H. L., Terlecky, S. R., Plant, C. P. & Dice, J. F. (1989). A role for a 70-kilodalton heat shock protein in lysosomal degradation of intracellular proteins. Science 246, 382-385. Comps, M., Trindade, M. & Delsert, C. L. (1996). Investigation of fish encephalitis viruses (FEV) expression in marine fishes using DIG-labelled probes. Aquaculture 143, 113-121. Cuervo, A. M. & Dice, J. F. (1996). A receptor for the selective uptake and degradation of proteins by lysosomes. Science 273, 501-503. Cuervo, A. M. & Dice, J. F. (2000). Unique properties of lamp2a compared to other lamp2 isoforms. Journal of Cell Science 113, 4441-4450. Cui, Q., Tashiro, S., Onodera, S. & Ikejima, T. (2006). Augmentation of oridonin-induced apoptosis observed with reduced autophagy. Journal of Pharmacological Sciences 101, 230-239. Dales, S., Eggers, H. J., Tamm, I. & Palade, G. E. (1965). Electron microscopic study of the formation of poliovirus. Virology 26, 379-389. Dasgupta, R., Ghosh, A., Dasmahapatra, B., Guarino, L. A. & Kaesberg, P. (1984). Primary and secondary structure of black beetle virus RNA2, the genomic messenger for BBV coat protein. Nucleic Acid Research 12, 7215-7223. Dasgupta, R. & Sgro, J. Y. (1989). Nucleotide sequences of three nodavirus RNA2’s : the messengers for their coat protein precursors. Nucleic Acid Research 17, 7525-7526. Dennis, P. B. , Jaeschke, A., Saitoh, M., Fowler, B., Kozma, S. C. & Thomas, G. (2001). Mammalian TOR: a homeostatic ATP sensor. Science 294, 1102-1105. Ellisen, L. W. (2005). Growth control under stress : mTOR regulation through the REDD1-TSC pathway. Cell Cycle 4, 1500-1502. Fenner, B. J., Goh, W. & Kwang, J. (2006a). Sequestration and protection of double-stranded RNA by the betanodavirus b2 protein. Journal of Virology 80, 6822-6833. Fenner, B. J., Thiagarajan, R., Chua, H. K. & Kwang, J. (2006b). Betanodavirus B2 is an RNA interference antagonist that facilitates intracellular viral RNA accumulation. Journal of Virology 80, 85-94. Fukuda, Y., Neuyen, H. D., Furuhashi, M. & Nakai, T. (1996). Mass mortality of cultured sevenband grouper, Epinephelus septemfasciatus, associated with viral nervous necrosis. Fish Pathology 31, 165-170. Furusawa, R., Okinaka, Y. & Nakai, T. (2006). Betanodavirus infection in the freshwater model fish medaka(Oryzias latipes). The Journal of General Virology 87, 2333-2339. Glazebrook, J. S., Heaaman, M. P., & de Beer, S. W. (1990). Picona-like viral particles associated with mass mortalities in larval barramundi, Lates calcarifer Bloch. Journal of Fish Diseases 13, 245-249. Gosert, R., Kanjanahaluethai, A., Egger, D., Bienz, K. & Baker, S. C. (2002). RNA replication of mouse hepatitis virus takes place at double-membrane vesicles. Journal of Virology 76, 3697-3708. Grotmol, S., Bergh, O. & Totland, G. K. (1999). Transmission of viral encephalopathy and retinopathy (VER) to yolk-sac larvae of the Atlantic halibut Hippoglossus hippoglossus: occurrence of nodavirus in various organs and a possible route of infection. Diseases of Aquatic Organisms 36, 95-106. Grotmol, S. & Totland, G. K. (2000). Surface disinfection of Atlantic halibut Hippoglossus hippoglossus eggs with ozonated sea-water inactives nodavirus and increases survival of the larvae. Diseases of Aquatic Organisms 39, 89-96. Grotmol, S., Totland, G. K., Kvellestad, A., Fjell, K. & Olsen, A. B. (1995). Mass mortality of larval and juvenile hatchery-reared halibut (Hippoglossus hippoglossus L.) associated with the presence of virus-like particles in vacuolated lesions in the central nervous system and retina. Bulletin of the European association of Fish Pathologists 15, 176-180. Grotmol, S., Totland, G. K., Thorud, K. & Hjeltnes, B. K. (1997). Vacuolating encephalopathy and retinopathy associated with a nodavirus-like agent: a probable cause of mass mortality of cultured larval and juvenile Atlantic halibut Hippoglossus hippoglossus. Diseases of Aquatic Organisms 29, 85-97. Guo, Y. X., Chan, S. W. & Kwang, J. (2004). Membrane association of greasy grouper nervous necrosis virus protein A and characterization of its mitochondrial localization targeting signal. Journal of Viology 78, 6498-6508. Hardie, D. G. (2007). AMP-activated/SNF1 protein kinases: conserved guardians of cellular energy. Nature Reviews Molecular Cell Biology 8, 774-785. He, C., Bartholomew, C. R., Zhou, W. & Klionsky, D. J. (2009). Assaying autophagic activity in transgenic GFP-Lc3 and GFP-Gabarap zebrafish embryos. Autophagy 5, 520-526. Hegde, A., Teh, H. C., Lam, T. J. & Sin, Y. M. (2003). Nodavirus infection in freshwater ornamental fish, guppy, Poicelia reticulate-comparative characterization and pathogenicity studies. Archieves of Virology 148, 575-586. Ichimura, Y., Imamura, Y., Emoto, K., Umeda, M., Noda, T. & Ohsumi, Y. (2004). In vivo and in vitro reconstitution of Atg8 conjugation essential for autophagy. The Journal of Biological Chemistry 279, 40584-40592. Ichimura, Y., Kirisako, T., Takao, T., Satomi, Y., Shimonishi, Y., Ishihara, N., Mizushima, N., Tanida, I., Kominami, E., Ohsumi, M., Noda, T. & Ohsumi, Y. (2000). A ubiquitin-like system mediates protein lipidation. Nature 408, 488-492. Jackson, W. T., Giddings, T. H., Taylor, M. P., Mulinyawe, S., Rabinovitch, M., Kopito, R. R. & Kirkegaard, K. (2005). Subversion of cellular autophagosomal machinery by RNA viruses. PLoS Biology 3, e156. Jiang, H., White, E. J., Gomez-Manzano, C. & Fueyo, J. (2008). Adenovirus’s last trick. Autophagy 4, 118-120. Kabeya, Y., Mizushima, N.,Ueno, T.,Yamamoto, A.,Kirisako, T.,Noda, T., Kominami, E.,Ohsumi, Y. & Yoshimori, T. (2003). LC3, a mammalian homologue of Yeast Apg8p,is localized in autophagosome membranes after processing. The EMBO Journal 19, 5720-5728. Kaesberg, P., Dasgupta, R., Sgro, J. Y., Wery, J. P., Selling, B. H., Hosur, M. V. &Johnson, J. E. (1990). Structural homology among four nodaviruses as dedused by sequencing and X-ray crystallography. Journal of Molecular Biology 214, 423-435. Kanazawa, T., Taneike, I., Akaishi, R., Yoshizawa, F., Furuya, N., Fujimura, S. & Kadowaki, M. (2004). Amino acids and insulin control autophagic proteolysis through different signaling pathways in relation to mTOR in isolated rat hepatocytes. The Journal of Biological Chemistry 279, 8452-8459. Kihara, A., Kabeya, Y., Ohsumi, Y. & Yoshimori, T. (2001). Beclin-phosphatidy- linositol 3-kinase complex functions at the trans-Golgi network. The EMBO Journal 2, 330– 335. Klionsky, D. J. (2005). The molecular machinery of autophagy: unanswered questions. Journal of Cell Science 118, 7-18. Klionsky, D. J. & Emr, S. D. (2000). Autophagy as a regulated pathway of cellular degradation. Science 290, 1717-1721. Kouroku, Y., Fujita, E., Tanida, I., Ueno, T., Isoai, A., Kumagai, H., Ogawa, S., Kaufman, R. J., Kominami, E. & Momoi, T. (2007). ER stress (PERK/eIF2 phosphorylation) mediates the polyglutamine-induced LC3 conversion, an essential step for autophagy formation. Cell Death and Differentiation 14, 230-239. Kuma, A., Hatano, M., Matsui, M., Yamamoto, A., Nakaya, H., Yoshimori, T., Ohsumi, Y., Tokuhisa, T. & Mizushima, N. (2004). The role of autophagy during the early neonatal starvation period. Nature 432, 1032-1036. Le Breton, A., Grisez, L., Sweetman, J., & Ollevier, F. (1997). Viral nervous necrosis (VNN) associated with mass mortalities in cage-reared sea bass, Dicentrarchus labrax (L.). Journal of Fish Diseases 20, 145-151. Lee, D. Y. & B. Sugden. (2008). The LMP1 oncogene of EBV activates PERK and the unfolded protein response to drive its own synthesis. Blood 111, 2280-2289. Lum, J. J., DeBerardinis, R. J. & Thompson, C. B. (2005). Autophagy in metazoans: cell survival in the land of plenty. Nature Reviews Molecular Cell Biology 6, 439-448. Mézeth, K. B., Nylund, S., Henriksen, H., Patel, S., Nerland, A. H. & Szilvay, A. M. (2007). RNA-dependent RNA polymerase from Atlantic halibut nodavirus contains two signals for localization to the mitochondria. Virus Research 130, 43-52. Massey, A. C., Zhang, C. & Cuervo, A. M. (2006). Chaperone-mediated autophagy in aging and disease. Current Topics in Developmental Biology 73, 205-235. Matsui, Y., Takagi, H., Qu, X., Abdellatif, M., Sakoda, H., Asano, T., Levine, B. & Sadoshima, J. (2007). Distinct Roles of Autophagy in the heart during ischemia and reperfusion: roles of AMP-activated protein kinase and beclin 1 in mediating autophagy. Circulation Research 100, 914-922. Mizushima, N. & Klionsky, D. J. (2007). Protein turnover via autophagy: implications for metabolism. Annual Review of Nutrition 27, 19-40. Mizushima, N., Noda, T., Yoshimori, T., Tanaka, Y., Ishii, T., George, M. D., Klionsky, D. J., Ohsumi, M. & Ohsumi, Y. (1998). A protein conjugation system essential for autophagy. Nature 395, 395-398. Mordier, S., Deval, C., Bechet, D., Tassa, A. & Ferrara, M. (2000). Leucine limitation induces autophagy and activation of lysosome-dependent proteolysis in C2C12 myotubes through a mammalian target of rapamycin-independent signaling pathway. The Joutnal of Biological Chemistry 275, 29900-29906. Mori, K., Nakai, T., Muroga, K., Arimoto, M., Mushiake, K. & Furusawa, I. (1992). Properties of a new virus belonging to nodaviridae found in larval striped jack (Pseudocaranx dentex) with nervous necrosis. Virology 187, 368-371. Mori, K., Nakai, T., Negahara, M., Muroga, K., Mckuchi, T. & Kanno, T. (1991). A viral disease in hatchery-reared larvae and juveniles of redspotted grouper. Fish Pathology 26, 209-210. Moriyasu, Y. & Ohsumi, Y. (1996). Autophagy in Tobacco suspension-cultured cells in response to sucrose starvation. Plant Physiology 111, 1233-1241. Mortimore, G. E. & Poso, A. R. (1987). Intracellular protein catabolism and its control during nutrient deprivation and supply. Annual Review of Nutrition 7, 539-568. Munday, B. L. & Nakai, T. (1997a). Special topic review: nodaviruses as pathogens in larval and juvenile marine finfish. World Journal of Microbiology and Biotechniology 13, 375-381. Munday, B. L. & Nakai, T. (1997b). Nodaviruses as pathogens in larval and juvenile marine finfish. World Journal of Microbiology and Biotechnology 13, 375-381. Munday, B. L., Kwang, J. & Moody, N. (2002). Betanodavirus infection of teleost fish : a review. Journal of Fish Diseases 25, 127-142. Munday, B. L., Langdon, J. S., Hyatt, A. & Humphrey, J. D. (1992). Mass mortality associated with a viral-induced vacuolating encephalophathy and retinopathy of larval and juvenile barramundi, Lates calcarifer Bloch. Aquaculture 130, 197-211. Muroga, K. (1995). Viral and bacterial disease in larval and juvenile marine fish and shellfish : a review. Fish Pathology 30, 71-85. Mushiake, K., Nishizawa, T., Nakai, T., Furusawa, I. & Muroga, K. (1994). Control of VNN in striped jack: selection of spawners based on the detection of SJNNV gene by polymerase chain reaction (PCR). Fish Pathology 29, 177-182. Nakai, T., Nguyen, H. D., Nishizawa, T., Muroga, K., Arimoto, M. & Ootsuki, K. (1994). Occurrence of viral nervous necrosis in kelp grouper and tiger puffer. Fish Pathology 29, 211-214. Nakashima, A., Tanaka, N., Tamai, K., Kyuuma, M., Ishikawa, Y., Sato, H., Yoshimori, T., Saito, S. & Sugamura, K. (2006). Survival of parvovirus B19-infected cells by cellular autophagy. Virology 349, 254-263. Nguyen, H. D., Nakai, T. & Muroga, K. (1996). Progression of striped jack nervous necrosis virus (SJNNV) in adult striped jack Pseudocaranx dentex larvae. Deseases of Aquatic Organisms 24, 99-105. Nishizawa, T., Furuhashi, M., Nagai, T., Nakai, T. & Muroga, K. (1997). Genomic classification of fish nodaviruses by molecular phylogenetic analysis of the coat protein gene. Applied and environmental microbiology 63, 1633-1636. Nishizawa, T., Mori, K., Furuhashi, M., Nakai, T., Furusawa, I. & Muroga, K. (1995). Comparison of the coat protein genes of five fish nodaviruses, the causative agent of nervous necrosis in marine fish. Journal of General Virology 76, 1563-1569. Nobukuni, T., Joaquin, M., Roccio, M., Dann, S. G., Kim, S. Y., Gulati, P., Byfield, M. P., Backer, J. M., Natt, F., Bos, J. L., Zwartkruis, F. J. T. & Thomas, G. (2005). Amino acids mediate mTOR/raptor signaling through activation of class 3 phosphatidylinositol 3OH-kinase. Proceedings of the National Academy of Sciences of the United States of America 102, 14238-14243. Noda, T. & Ohsumi, Y. (1998). Tor, a phosphatidylinositol kinase homologue, controls autophagy in yeast. The Journal of Biological Chemistry 273, 3963-3966. Nylund, A., Karlsbakk, E., Nylund, S., Isaksen, T. E., Karlsen, M., Korsnes, K., Handeland, S., Martinsen, R., Mork Pedersen, T. & Ottem, K. F. (2008). New clade of betanodaviruses detected in wild and farmed cod (Gadus morhua) in Norway. Archives of Virology 153, 541-547. Ogata, M., S. Hino, A. Saito, K. Morikawa, S. Kondo, S. Kanemoto, T., Murakami, M. T., I. Tanii, K. Yoshinaga, S. Shiosaka, J. A., and Hammarback, F. U. & K. Imaizumi. (2006). Autophagy is activated for cell survival after endoplasmic reticulum stress. Molecular and Cellular Biology 26, 9220-9231. Onodera, J. & Ohsumi, Y. (2005). Autophagy is required for maintenance of amino acid levels and protein synthesis under nitrogen starvation. The Journal of Biological Chemistry 280, 31582-31586. Ou, M. C., Chen, Y. M., Jeng, M. F., Chu, C. J., Yang, H. L. & Chen, T. Y. (2007). Identification of critical residues in nervous necrosis virus B2 for dsRNA-binding and RNAi-inhibiting activity through by bioinformatic analysis and mutagenesis. Biochemical and Biophysical Research Communications 361, 634-640. Pedersen, K. W., van der Meer, Y., Roos, N. & Snijder, E. J. (1999). Open reading frame 1a-encoded subunits of the arterivirus replicase induce endoplasmic reticulum-derived double-membrane vesicles which carry the viral replication complex. Journal of Virology 73, 2016-2026. Petiot, A., Ogier-Denis, E., Blommaart, E. F. C., Meijer, A. J. & Codogno, P. (2000). Distinct classes of phosphatidylinositol 3'-kinases are involved in signaling pathways that control macroautophagy in HT-29 cells. The Journal of Biological Chemistry 275, 992-998. Prentice, E., Jerome, W. G., Yoshimori, T., Mizushima, N. & Denison, M. R. (2004). Coronavirus replication complex formation utilizes components of cellular autophagy. The Journal of Biological Chemistry 279, 10136-10141. Qu, X., Zou, Z., Sun, Q., Luby-Phelps, K., Cheng, P., Hogan,R.N., Gilpin, C. & Levine, B. (2007). Autophagy gene-dependent clearance of apoptotic cells during embryonic development. Cell 128, 931-946. Ravikumar, B., Vacher, C., Berger, Z., Davies, J. E., Luo, S., Oroz, L. G., Scaravilli, F., Easton, D. F., Duden, R., O'Kane, C. J. & Rubinsztein, D. C. (2004). Inhibition of mTOR induces autophagy and reduces toxicity of polyglutamine expansions in fly and mouse models of Huntington disease. Nature Genetics 36, 585-595. Renault, T., Haffner, P. H., Baudin, L. F., Breuil, G. & Bonami, J. R. (1991). Mass mortalities in hatchery-reared sea bass( Lates calcarifer )larvae associated with the presence in the brain and retina of virus-like particles. Bulletin of the European association of Fish Pathologists 11, 69-73. Sarbassov, D. D., Ali, S. M., Sabatini, D. M. (2005). Growing roles for the mTOR pathway. Current Opinion in Cell Biology 17, 596-603. Sarkar, S., Perlstein, E. O., Imarisio, S., Pineau, S., Cordenier, A., Maglathlin, R. L., Webster, J. A., Lewis, T. A., O'Kane, C. J., Schreiber, S. L. & Rubinsztein, D. C. (2007). Small molecules enhance autophagy and reduce toxicity in Huntington's disease models. Nature Chemical Biology 3, 331-338. Schlegel, A., Giddings, T. H. Jr., Ladinsky, M. S. & Kirkegaard, K. (1996). Cellular origin and ultrastructure of membranes induced during poliovirus infection. The Journal of Virology 70, 6576-6588. Seglen, P. O. & Gordon, P. B. (1982). 3-Methyladenine: Specific inhibitor of autophagic/lysosomal protein degradation in isolated rat hepatocytes. Cell Biology 79, 1889-1892. Shintani, T., Mizushima, N., Ogawa, Y., Matsuura, A., Noda, T. & Ohsumi, Y. (1999). Apg10p, a novel protein-conjugating enzyme essential for autophagy in yeast. The EMBO Journal 18, 5234-5241. Sir, D., Chen, W. L., Choi, J., Wakita, T., Yen, T. S. & Ou, J. H. (2008). Induction of incomplete autophagic response by hepatitis C virus via the unfolded protein response. Hepatology 48, 1054-1061. Skliris, G. P. & Richards, R. H. (1998). Assessment of the susceptibility of the brine shrimp Artemia salina and rotifer Brachiomus plicatilis to experimental nodavirus infection. Aquaculture 169, 133-141. Snijder, E. J., van Tol, H., Roos, N. & Pedersen, K. W. (2001). Non-structural proteins 2 and 3 interact to modify host cell membranes during the formation of the arterivirus replication complex. Journal of General Virology 82, 985-994. Starkey, W. G. (2000). Find more like this isolation of nodavirus from Scottish farmed halibut, Hippoglossus hippoglossus (L). Journal of Fish Disease 23, 419-422. Su, Y. C., Wu, J. L. & Hong, J. R. (2009). Betanodavirus non-structural protein B2: A novel necrotic death factor that induces mitochondria-mediated cell death in fish cells. Virology 385, 143-154. Suhy, D. A., Giddings, T. H. Jr. & Kirkegaard, K. (2000). Remodeling the endoplasmic reticulum by poliovirus infection and by individual viral proteins: an autophagy-like origin for virus-induced vesicles. The Journal of Virology 74, 8953-8965. Suzuki, K. & Ohsumi, Y. (2007). Molecular machinery of autophagosome formation in yeast, Saccharomyces cerevisiae. FEBS Letters 581, 2156-2161. Takeshige, K., Baba, M., Tsuboi, S., Noda, T. & Ohsumi, Y. (1992). Autophagy in yeast demonstrated with proteinase-deficient mutants and conditions for its induction. The Journal of Cell Biology 119, 301-311. Talloczy, Z., Jiang, W., Virgin, H. W., Leib, D. A., Scheuner, D., Kaufman, R. J., Eskelinen, E. L. & Levine, B. (2002). Regulation of starvation- and virus-induced autophagy by the eIF2alpha kinase signaling pathway. Proceedings of the National Academy of Sciences of the United States of America 99, 190-195. Tanida, I., Tanida-Miyake, E., Ueno, T. & Kominami, E. (2001). The human homolog of Saccharomyces cerevisiae Apg7p is a protein-activating enzyme for multiple substrates including Human Apg12p, GATE-16, GABARAP, and MAP-LC3. The Journal of Biological Chemistry 276, 1701-1706. Taylor, M. P. & Jackson, W. T. (2009). Viruses and arrested autophagosome development. Autophagy 5, 870-871. Tsukada, M. & Ohsumi, Y. (1993). Isolation and characterization of autophagy- defective mutants of Saccharomyces cerevisiae. FEBS Letters 333, 169-174. van der Meer, Y., Snijder, E. J., Dobbe, J. C., Schleich, S., Denison, M. R., Spaan, W. J. M. & Locker, J. K. (1999). Localization of mouse hepatitis virus nonstructural proteins and RNA synthesis indicates a role for late endosomes in viral replication. The Journal of Virology 73, 7641-7657. van der Meer, Y., van Tol, H., Krijnse Locker, J. & Snijder, E. J. (1998). ORF1a-encoded replicase subunits are involved in the membrane association of the arterivirus replication complex. The Journal of Virology 72, 6689-6698. Yorimitsu, T. & D. J. Klionsky. (2007). Eating the endoplasmic reticulum: quality control by autophagy. Trends in Cell Biology 17, 279-285. Yoshikoshi, K. & Inoue, K. (1990). Viral nervous necrosis in hatchery-reared larve and juveniles of Japanese parrotfish,Oplegnathus fasciatus and juveniles of Japanese parrotfish,Oplegnathus fasciatus. Journal of Fish Diseases 13, 69-77. Yoshimoto, K., Hanaoka, H., Sato, S., Kato, T., Tabata, S., Noda, T. & Ohsumi, Y. (2004). Processing of ATG8s, ubiquitin-like proteins, and their deconjugation by ATG4s are essential for plant autophagy. Plant Cell 16, 2967-2983. Yu, L., Alva, A., Su, H., Dutt, P., Freundt, E., Welsh, S., Baehrecke, E. H. &Lenardo, M. J. (2004). Regulation of an ATG7-beclin 1 program of autophagic cell death by caspase-8. Science 304, 1500-1502. Zeng, X., Overmeyer, J. H. & Maltese, W. A. (2006). Functional specificity of the mammalian Beclin-Vps34 PI 3-kinase complex in macroautophagy versus endocytosis and lysosomal enzyme trafficking. Journal of Cell Science 119, 259-270. Zorriehzahra, M. J. (2005). Mortality of wild Golden Grey Mullet (Liza auratus) in Iranian waters of the Caspian Sea, associated with viral nervous necrosis-like agent. Iranian Joural of Fisheries Sciences 4, 43-58. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/43726 | - |
dc.description.abstract | 神經壞死症病毒(NNV)屬於單股正意RNA病毒, NNV感染細胞後會造成粒線體膜電位的流失。自噬體(autophagosome)是細胞在饑餓狀態時形成的雙層膜囊狀構造,可將長效型蛋白質及胞器分解產生胺基酸及ATP,以調控細胞內的能量。本篇研究首次在魚類細胞中發現NNV與細胞自噬之間交互影響的機制。細胞轉殖pEGFP-LC3質體後再感染NNV,可在細胞內觀察到具螢光的細胞自噬體。運用luciferase氧化ATP所放出的冷光量作定量分析,發現細胞在病毒感染後12小時內,ATP會逐漸下降;當病毒感染濃度越高,ATP量則下降越多。用cycloheximide抑制病毒蛋白質的轉譯,ATP量就恢復到未感染時狀態,因此NNV蛋白質的轉譯會造成受病毒感染細胞ATP量的下降。另外,當細胞被高濃度NNV感染時,會出現大量的自噬體,當NNV蛋白轉譯被CHX抑制時,自噬體數量就明顯減少,因此NNV蛋白轉譯會誘發自噬體數量增加。以饑餓法使細胞內ATP量先大幅降低再感染NNV,結果饑餓組細胞內的NNV蛋白量比正常細胞感染NNV組的少,但自噬體數量卻相對較多,因此推論,NNV感染細胞所誘發的自噬作用主要是因NNV造成ATP下降所引起,而不需經由NNV蛋白質過多造成的ER stress所誘發。當自噬體的功能被抑制時,受感染細胞的病毒產量則明顯下降,因此推論,自噬作用有助於病毒的複製,但免疫螢光染色結果顯示, NNV並不會在細胞自噬體膜上形成複製複合體。細胞感染病毒後,再用三種藥劑分別去抑制細胞自噬作用,結果藥物處理組細胞內的ATP量比未加藥劑組低,病毒力價亦較低。當飢餓細胞的自噬作用被藥物抑制時,細胞內的ATP量大幅減少,感染NNV後所產生的病毒量也比未加抑制劑組少,因此推測,病毒感染所誘發的自噬作用,有助於細胞回收蛋白質及胞器來產生ATP,供應病毒複製之用。 | zh_TW |
dc.description.abstract | Nervous necrosis virus (NNV) is a positive single-stranded RNA virus. NNV infection was reported to induce the loss of mitochondria membrane potential. Autophagy is a unique membrane traffic system for engulfing long-lived proteins and organelles to maintain homeostasis of cellular energy during starvation. This research first reported the interaction between NNV and autophagy in fish cells. After transfection of pEGFP-LC3 plasmids, fluorescence of autophagosomes was observed in NNV-infected cells. Quantitative analysis of ATP by chemoiluminescence, ATP was found to decrease gradually after NNV infection. When viral protein translation was inhibited by cycloheximide (CHX) treatment, the level of ATP restored to the original level of non-infected cells, indicating that NNV replication could decrease ATP of infected cell. By the way, the level of autophagy could be induced by NNV infection, but it was down-regulated when viral protein translation was inhibited by CHX treatment. When the cells were pre-starved to reduce the level of ATP and then infected with NNV, the level of autophagy was higher but the level of viral protein was lower than that in the NNV-infected cells without pre-starvation. Therefore, it is suggested that the up-regulation of autophagosomes in NNV-infected cells was much related with the loss of ATP and less related with ER stress. When NNV-infected cells were separately treated with three autophagy inhibitors, the levels of ATP and the levels of progeny viral titers were lower than that of untreated NNV-infected cells. All the above data suggested that NNV-induced autophagy was helpful to product the cellular ATP and provide the resources for viral replication. | en |
dc.description.provenance | Made available in DSpace on 2021-06-15T02:26:57Z (GMT). No. of bitstreams: 1 ntu-98-R96b41018-1.pdf: 1177855 bytes, checksum: d4a125b3ffe7d875d3d2d5a9b4f1033e (MD5) Previous issue date: 2009 | en |
dc.description.tableofcontents | 口試委員會審定書 ………………………………I
誌謝…………………………………………………II 中文摘要 ……………………………………… III 英文摘要 ……………………………………… IV 目錄 ………………………………………… V 圖目錄 ………………………… VIII 第一章 緒言…………………………………………1 1. 魚類神經壞死症病毒 ………………………… 1 1.1. 魚類神經壞死症之病史與病癥………………… 1 1.2. 神經壞死症病毒的特性及分類地位………… 1 1.3. 神經壞死症病毒地理分布和宿主範圍……… 3 1.4. 神經壞死症病毒的傳染途徑………………… 4 1.5. 神經壞死症病毒散佈之病程及在宿主體內之分布……5 2. 細胞自噬體……………………………………………6 2.1. 細胞自噬體的功能………………………………6 2.2. 細胞自噬體的引發………………………………7 2.3. 細胞自噬體的形成…………………………………9 3. 細胞自噬體和病毒的關聯……………………………9 3.1. 病毒引起的細胞自噬反應………………………………9 3.2. 自噬體的產生有助於病毒增殖的機制…………………10 4. 研究目的………………………………………………11 第二章 材料與方法……………………………………12 1. 轉殖進pEGFP-LC3質體的GF-1細胞株建立……………12 1.1. pEGFP-LC3質體的轉殖..…………………………12 1.2. 轉殖進pEGFP-LC3質體的細胞株選殖………………12 2. GF-1細胞株感染HGNNV後引起自噬反應的機制………13 2.1. GF-1細胞株感染HGNNV後ATP量隨時間的變化………13 2.2. GF-1細胞株感染不同濃度HGNNV後ATP量的變化…14 2.3. 轉譯抑制對感染細胞內ATP量的影響.……………14 2.4. 感染HGNNV對細胞內粒線體膜電位的影響………15 2.5. 轉殖進pEGFP-LC3質體之GF-1細胞株感染HGNNV後的自噬體觀察......16 2.6. 轉殖GF-1細胞株感染不同濃度HGNNV後自噬體的數量變化…16 2.7. 轉譯抑制對感染細胞內自噬體數量的影響……………16 2.8. 飢餓狀態與抑制自噬作用對起始ATP濃度的影響……………17 2.9. 起始ATP濃度對感染細胞自噬體數量的影響…………………17 2.10. 西方墨漬法 ( Western immmunoblot )……………………18 3. 自噬體促進病毒增殖機制………………………………………22 3.1. 抑制自噬體對病毒產量的影響………………………………22 3.2. 自噬體對胞外病毒產量的影響….…………………………22 3.3. 抑制自噬體對感染細胞內ATP濃度的影響…………………23 3.4. ATP濃度對病毒產量的影響…………………………………24 3.5. 飢餓狀態下抑制自噬作用對起始ATP濃度的影響…………25 3.6. 起始ATP濃度對病毒產量的影響……………………………25 3.7. Methyl pyruvate對抑制自噬體後HGNNV產量的影響……26 4. NNV和自噬體的直接關連……………………………………26 4.1. HGNNV的RdRp和自噬體及粒線體的位置比較……………26 4.2. NNV誘發的自噬體和溶小體結合的情形………………27 第三章 結果 ………………………………………………29 1. NNV感染會誘發自噬作用………………………………29 1.1. NNV感染造成細胞內ATP量下降………………………29 1.2. 細胞內ATP量下降會誘發細胞自噬作用…………………30 2. 細胞自噬作用有助於NNV的增殖……………………………31 2.1. 自噬作用對病毒的影響…………………………………31 2.2. 自噬作用產生的ATP有助於NNV的增殖…………………32 3. NNV和誘發的自噬體間的直接關連………………………33 3.1. NNV的RdRp與自噬體及粒線體間的位置比較………33 3.2. NNV誘發出的自噬體與溶小體結合的情形…………34 第四章 討論 …………………………………………………35 1. NNV感染使細胞內ATP下降………………………………35 2. ATP下降誘發細胞自噬作用………………………………36 3. NNV引發的自噬作用有助ATP生成………………………37 4. ATP幫助NNV增殖…………………………………………39 5. 未來展望…………………………………………………40 第五章 參考文獻……………………………………………41 | |
dc.language.iso | zh-TW | |
dc.title | 魚類神經壞死症病毒與細胞自噬的交互作用機制 | zh_TW |
dc.title | Interaction between betanodavirus and autophagy | en |
dc.type | Thesis | |
dc.date.schoolyear | 97-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 莊寧寧(Nin-Nin Chuang),黃偉邦(Wei-Pang Huang),張麗冠(Li-Kwan Chang) | |
dc.subject.keyword | 魚類神經壞死症病毒,粒線體膜電位,自噬體,ATP,自噬體抑制劑, | zh_TW |
dc.subject.keyword | nervous necrosis virus,mitochondrial membrane potential,autophagy,ATP,autophagy inhibitors, | en |
dc.relation.page | 74 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2009-08-17 | |
dc.contributor.author-college | 生命科學院 | zh_TW |
dc.contributor.author-dept | 動物學研究所 | zh_TW |
顯示於系所單位: | 動物學研究所 |
文件中的檔案:
檔案 | 大小 | 格式 | |
---|---|---|---|
ntu-98-1.pdf 目前未授權公開取用 | 1.15 MB | Adobe PDF |
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。