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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 張麗冠(Li-Kwan Chang) | |
dc.contributor.author | Ping-Han Huang | en |
dc.contributor.author | 黃柄翰 | zh_TW |
dc.date.accessioned | 2021-06-15T02:22:19Z | - |
dc.date.available | 2012-08-22 | |
dc.date.copyright | 2011-08-22 | |
dc.date.issued | 2011 | |
dc.date.submitted | 2011-08-17 | |
dc.identifier.citation | Attasart P, Kaewkhaw R, Chimwai C, Kongphom U, Namramoon O, Panyim S (2009) Inhibition of white spot syndrome virus replication in Penaeus monodon by combined silencing of viral rr2 and shrimp PmRab7. Virus Res 145(1): 127-133
Bhende PM, Dickerson SJ, Sun X, Feng WH, Kenney SC (2007) X-box-binding protein 1 activates lytic Epstein-Barr virus gene expression in combination with protein kinase D. J Virol 81(14): 7363-7370 Bieker JJ (2001) Kruppel-like factors: three fingers in many pies. J Biol Chem 276(37): 34355-34358 Borras AM, Strominger JL, Speck SH (1996) Characterization of the ZI domains in the Epstein-Barr virus BZLF1 gene promoter: role in phorbol ester induction. J Virol 70(6): 3894-3901 Brayer KJ, Segal DJ (2008) Keep your fingers off my DNA: protein-protein interactions mediated by C2H2 zinc finger domains. Cell Biochem Biophys 50(3): 111-131 Chang HS, Lin CH, Yang CH, Liang YJ, Yu WC The human papillomavirus-16 (HPV-16) oncoprotein E7 conjugates with and mediates the role of the transforming growth factor-beta inducible early gene 1 (TIEG1) in apoptosis. Int J Biochem Cell Biol 42(11): 1831-1839 Chang LK, Chung JY, Hong YR, Ichimura T, Nakao M, Liu ST (2005) Activation of Sp1-mediated transcription by Rta of Epstein-Barr virus via an interaction with MCAF1. Nucleic Acids Res 33(20): 6528-6539 Chen X, Bieker JJ (2004) Stage-specific repression by the EKLF transcriptional activator. Mol Cell Biol 24(23): 10416-10424 Chen YH, Jia XT, Huang XD, Zhang S, Li M, Xie JF, Weng SP, He JG (2011) Two Litopenaeus vannamei HMGB proteins interact with transcription factors LvSTAT and LvDorsal to activate the promoter of white spot syndrome virus immediate-early gene ie1. Mol Immunol 48(5): 793-799 Chou HY, Huang CY, Wang CH, Chiang HC, Lo CF (1995) Pathogenicity of a baculovirus infection causing white spot syndrome in cultured penaeid shrimp in Taiwan. Dis Aquat Organ 23: 165-173 Cox MA, Leahy J, Hardwick JM (1990) An enhancer within the divergent promoter of Epstein-Barr virus responds synergistically to the R and Z transactivators. J Virol 64(1): 313-321 Dang DT, Pevsner J, Yang VW (2000) The biology of the mammalian Kruppel-like family of transcription factors. Int J Biochem Cell Biol 32(11-12): 1103-1121 Dostert C, Jouanguy E, Irving P, Troxler L, Galiana-Arnoux D, Hetru C, Hoffmann JA, Imler JL (2005) The Jak-STAT signaling pathway is required but not sufficient for the antiviral response of drosophila. Nat Immunol 6(9): 946-953 Dynan WS, Tjian R (1983) The promoter-specific transcription factor Sp1 binds to upstream sequences in the SV40 early promoter. Cell 35(1): 79-87 Evans PM, Zhang W, Chen X, Yang J, Bhakat KK, Liu C (2007) Kruppel-like factor 4 is acetylated by p300 and regulates gene transcription via modulation of histone acetylation. J Biol Chem 282(47): 33994-34002 Feederle R, Kost M, Baumann M, Janz A, Drouet E, Hammerschmidt W, Delecluse HJ (2000) The Epstein-Barr virus lytic program is controlled by the co-operative functions of two transactivators. EMBO J 19(12): 3080-3089 Feng WC, Southwood CM, Bieker JJ (1994) Analyses of beta-thalassemia mutant DNA interactions with erythroid Kruppel-like factor (EKLF), an erythroid cell-specific transcription factor. J Biol Chem 269(2): 1493-1500 Flamand L, Menezes J (1996) Cyclic AMP-responsive element-dependent activation of Epstein-Barr virus zebra promoter by human herpesvirus 6. J Virol 70(3): 1784-1791 Flegel TW (1997) Special topic review: major viral diseases of black tiger prawn (Penaeus monodon) in Thailand. World J Microbiol Biotechnol 13: 433-442 Flemington E, Speck SH (1990) Autoregulation of Epstein-Barr virus putative lytic switch gene BZLF1. J Virol 64(3): 1227-1232 Flemington EK, Goldfeld AE, Speck SH (1991) Efficient transcription of the Epstein-Barr virus immediate-early BZLF1 and BRLF1 genes requires protein synthesis. J Virol 65(12): 7073-7077 Friedman A, Perrimon N (2004) Genome-wide high-throughput screens in functional genomics. Curr Opin Genet Dev 14(5): 470-476 Friesen PD, Miller LK (1986) The regulation of baculovirus gene expression. Curr Top Microbiol Immunol 131: 31-49 Funnell AP, Maloney CA, Thompson LJ, Keys J, Tallack M, Perkins AC, Crossley M (2007) Erythroid Kruppel-like factor directly activates the basic Kruppel-like factor gene in erythroid cells. Mol Cell Biol 27(7): 2777-2790 Furnari FB, Adams MD, Pagano JS (1992) Regulation of the Epstein-Barr virus DNA polymerase gene. J Virol 66(5): 2837-2845 Glaser G, Vogel M, Wolf H, Niller HH (1998) Regulation of the Epstein-Barr viral immediate early BRLF1 promoter through a distal NF1 site. Arch Virol 143(10): 1967-1983 Granato M, Farina A, Gonnella R, Santarelli R, Frati L, Faggioni A, Angeloni A (2006) Regulation of the expression of the Epstein-Barr virus early gene BFRF1. Virology 347(1): 109-116 Hammond SM, Bernstein E, Beach D, Hannon GJ (2000) An RNA-directed nuclease mediates post-transcriptional gene silencing in Drosophila cells. Nature 404(6775): 293-296 Hammond SM, Caudy AA, Hannon GJ (2001) Post-transcriptional gene silencing by double-stranded RNA. Nat Rev Genet 2(2): 110-119 Honess RW, Roizman B (1974) Regulation of herpesvirus macromolecular synthesis. I. Cascade regulation of the synthesis of three groups of viral proteins. J Virol 14(1): 8-19 Huang J, Liao G, Chen H, Wu FY, Hutt-Fletcher L, Hayward GS, Hayward SD (2006) Contribution of C/EBP proteins to Epstein-Barr virus lytic gene expression and replication in epithelial cells. J Virol 80(3): 1098-1109 Huang XD, Zhao L, Zhang HQ, Xu XP, Jia XT, Chen YH, Wang PH, Weng SP, Yu XQ, Yin ZX, He JG (2010) Shrimp NF-kappaB binds to the immediate-early gene ie1 promoter of white spot syndrome virus and upregulates its activity. Virology 406(2): 176-180 Hung CH, Liu ST (1999) Characterization of the Epstein-Barr virus BALF2 promoter. J Gen Virol 80 ( Pt 10): 2747-2750 Inouye K, Miwa S, Oseko N, Nakano H, Kimura T, Momoyama K, Hiraoka M (1994) Mass mortalities of cultured kuruma shrimp Penaeus japonicus in Japan in 1993: electron microscopic evidence of the causative virus. Fish Pathol 29: 149-158 Kaczynski J, Cook T, Urrutia R (2003) Sp1- and Kruppel-like transcription factors. Genome Biol 4(2): 206 Kaczynski J, Zhang JS, Ellenrieder V, Conley A, Duenes T, Kester H, van Der Burg B, Urrutia R (2001) The Sp1-like protein BTEB3 inhibits transcription via the basic transcription element box by interacting with mSin3A and HDAC-1 co-repressors and competing with Sp1. J Biol Chem 276(39): 36749-36756 Kaczynski JA, Conley AA, Fernandez Zapico M, Delgado SM, Zhang JS, Urrutia R (2002) Functional analysis of basic transcription element (BTE)-binding protein (BTEB) 3 and BTEB4, a novel Sp1-like protein, reveals a subfamily of transcriptional repressors for the BTE site of the cytochrome P4501A1 gene promoter. Biochem J 366: 873-882 Kadam S, McAlpine GS, Phelan ML, Kingston RE, Jones KA, Emerson BM (2000) Functional selectivity of recombinant mammalian SWI/SNF subunits. Genes Dev 14(19): 2441-2451 Kadonaga JT, Carner KR, Masiarz FR, Tjian R (1987) Isolation of cDNA encoding transcription factor Sp1 and functional analysis of the DNA binding domain. Cell 51(6): 1079-1090 Kenney S, Holley-Guthrie E, Mar EC, Smith M (1989) The Epstein-Barr virus BMLF1 promoter contains an enhancer element that is responsive to the BZLF1 and BRLF1 transactivators. J Virol 63(9): 3878-3883 Kolman JL, Taylor N, Gradoville L, Countryman J, Miller G (1996) Comparing transcriptional activation and autostimulation by ZEBRA and ZEBRA/c-Fos chimeras. J Virol 70(3): 1493-1504 Laub F, Lei L, Sumiyoshi H, Kajimura D, Dragomir C, Smaldone S, Puche AC, Petros TJ, Mason C, Parada LF, Ramirez F (2005) Transcription factor KLF7 is important for neuronal morphogenesis in selected regions of the nervous system. Mol Cell Biol 25(13): 5699-5711 Le Roux F, Sergeant A, Corbo L (1996) Epstein-Barr virus (EBV) EB1/Zta protein provided in trans and competent for the activation of productive cycle genes does not activate the BZLF1 gene in the EBV genome. J Gen Virol 77 ( Pt 3): 501-509 Lee JS, Ngo H, Kim D, Chung JH (2000) Erythroid Kruppel-like factor is recruited to the CACCC box in the beta-globin promoter but not to the CACCC box in the gamma-globin promoter: the role of the neighboring promoter elements. Proc Natl Acad Sci U S A 97(6): 2468-2473 Leu JH, Yang F, Zhang X, Xu X, Kou GH, Lo CF (2009) Whispovirus. Curr Top Microbiol Immunol 328: 197-227 Li D, Yea S, Dolios G, Martignetti JA, Narla G, Wang R, Walsh MJ, Friedman SL (2005) Regulation of Kruppel-like factor 6 tumor suppressor activity by acetylation. Cancer Res 65(20): 9216-9225 Li DF, Zhang MC, Yang HJ, Zhu YB, Xu X (2007) Beta-integrin mediates WSSV infection. Virology 368(1): 122-132 Li F, Li M, Ke W, Ji Y, Bian X, Yan X (2009) Identification of the immediate-early genes of white spot syndrome virus. Virology 385(1): 267-274 Licht JD, Grossel MJ, Figge J, Hansen UM (1990) Drosophila Kruppel protein is a transcriptional repressor. Nature 346(6279): 76-79 Lin F, Huang H, Xu L, Li F, Yang F (2011) Identification of three immediate-early genes of white spot syndrome virus. Arch Virol Lin YR, Hung HC, Leu JH, Wang HC, Kou GH, Lo CF (2011) The role of aldehyde dehydrogenase and hsp70 in suppression of white spot syndrome virus replication at high temperature. J Virol 85(7): 3517-3525 Liu P, Speck SH (2003) Synergistic autoactivation of the Epstein-Barr virus immediate-early BRLF1 promoter by Rta and Zta. Virology 310(2): 199-206 Liu S, Borras AM, Liu P, Suske G, Speck SH (1997a) Binding of the ubiquitous cellular transcription factors Sp1 and Sp3 to the ZI domains in the Epstein-Barr virus lytic switch BZLF1 gene promoter. Virology 228(1): 11-18 Liu S, Liu P, Borras A, Chatila T, Speck SH (1997b) Cyclosporin A-sensitive induction of the Epstein-Barr virus lytic switch is mediated via a novel pathway involving a MEF2 family member. EMBO J 16(1): 143-153 Liu WJ, Chang YS, Huang WT, Chen IT, Wang KC, Kou GH, Lo CF (2011) Penaeus monodon TATA Box-Binding Protein Interacts with the White Spot Syndrome Virus Transactivator IE1 and Promotes Its Transcriptional Activity. J Virol 85(13): 6535-6547 Liu WJ, Chang YS, Wang AH, Kou GH, Lo CF (2007) White spot syndrome virus annexes a shrimp STAT to enhance expression of the immediate-early gene ie1. J Virol 81(3): 1461-1471 Liu WJ, Chang YS, Wang CH, Kou GH, Lo CF (2005) Microarray and RT-PCR screening for white spot syndrome virus immediate-early genes in cycloheximide-treated shrimp. Virology 334(2): 327-341 Liu WJ, Chang YS, Wang HC, Leu JH, Kou GH, Lo CF (2008) Transactivation, dimerization, and DNA-binding activity of white spot syndrome virus immediate-early protein IE1. J Virol 82(22): 11362-11373 Lo CF, Ho CH, Peng SE, Chen CH, Hsu HC, Chiu YL, Chiu CF, Chang CF, Liu KF, Su MS, Wang CH, Kou GH (1996) White spot syndrome baculovirus (WSBV) detected in cultured and captured shrimp, crabs and other arthropods. Dis Aquat Organ 27: 215-225 Lu CC, Jeng YY, Tsai CH, Liu MY, Yeh SW, Hsu TY, Chen MR (2006) Genome-wide transcription program and expression of the Rta responsive gene of Epstein-Barr virus. Virology 345(2): 358-372 Magbanua FO, Natividad KT, Migo VP, Alfafara CG, de la Pena FO, Miranda RO, Albaladejo JD, Nadala EC, Jr., Loh PC, Mahilum-Tapay L (2000) White spot syndrome virus (WSSV) in cultured Penaeus monodon in the Philippines. Dis Aquat Organ 42(1): 77-82 Matsumoto N, Kubo A, Liu H, Akita K, Laub F, Ramirez F, Keller G, Friedman SL (2006) Developmental regulation of yolk sac hematopoiesis by Kruppel-like factor 6. Blood 107(4): 1357-1365 McConnell BB, Yang VW (2010) Mammalian Kruppel-like factors in health and diseases. Physiol Rev 90(4): 1337-1381 Mehta TS, Lu H, Wang X, Urvalek AM, Nguyen KH, Monzur F, Hammond JD, Ma JQ, Zhao J (2009) A unique sequence in the N-terminal regulatory region controls the nuclear localization of KLF8 by cooperating with the C-terminal zinc-fingers. Cell Res 19(9): 1098-1109 Mejia-Ruiz CH, Vega-Pena S, Alvarez-Ruiz P, Escobedo-Bonilla CM (2010) Double-stranded RNA against white spot syndrome virus (WSSV) vp28 or vp26 reduced susceptibility of Litopenaeus vannamei to WSSV, and survivors exhibited decreased susceptibility in subsequent re-infections. J Invertebr Pathol 107(1): 65-68 Miller IG, Jr., El-Guindy A (2002) Regulation of Epstein-Barr virus lytic cycle activation in malignant and nonmalignant disease. J Natl Cancer Inst 94(23): 1733-1735 Miller IJ, Bieker JJ (1993) A novel, erythroid cell-specific murine transcription factor that binds to the CACCC element and is related to the Kruppel family of nuclear proteins. Mol Cell Biol 13(5): 2776-2786 Miyamoto S, Suzuki T, Muto S, Aizawa K, Kimura A, Mizuno Y, Nagino T, Imai Y, Adachi N, Horikoshi M, Nagai R (2003) Positive and negative regulation of the cardiovascular transcription factor KLF5 by p300 and the oncogenic regulator SET through interaction and acetylation on the DNA-binding domain. Mol Cell Biol 23(23): 8528-8541 Mohan CV, Shankar KM, Kulkarni S, Sudha PM (1998) Histopathology of cultured shrimp showing gross signs of yellow head syndrome and white spot syndrome during 1994 Indian epizootics. Dis Aquat Organ 34(1): 9-12 Oates AC, Pratt SJ, Vail B, Yan Y, Ho RK, Johnson SL, Postlethwait JH, Zon LI (2001) The zebrafish klf gene family. Blood 98(6): 1792-1801 Ongvarrasopone C, Chanasakulniyom M, Sritunyalucksana K, Panyim S (2008) Suppression of PmRab7 by dsRNA inhibits WSSV or YHV infection in shrimp. Mar Biotechnol (NY) 10(4): 374-381 Pandya K, Townes TM (2002) Basic residues within the Kruppel zinc finger DNA binding domains are the critical nuclear localization determinants of EKLF/KLF-1. J Biol Chem 277(18): 16304-16312 Park JH, Lee YS, Lee S, Lee Y (1998) An infectious viral disease of penaeid shrimp newly found in Korea. Dis Aquat Organ 34(1): 71-75 Pearson R, Fleetwood J, Eaton S, Crossley M, Bao S (2008) Kruppel-like transcription factors: a functional family. Int J Biochem Cell Biol 40(10): 1996-2001 Philipsen S, Suske G (1999) A tale of three fingers: the family of mammalian Sp/XKLF transcription factors. Nucleic Acids Res 27(15): 2991-3000 Quinlivan EB, Holley-Guthrie EA, Norris M, Gutsch D, Bachenheimer SL, Kenney SC (1993) Direct BRLF1 binding is required for cooperative BZLF1/BRLF1 activation of the Epstein-Barr virus early promoter, BMRF1. Nucleic Acids Res 21(14): 1999-2007 Ragoczy T, Miller G (1999) Role of the epstein-barr virus RTA protein in activation of distinct classes of viral lytic cycle genes. J Virol 73(12): 9858-9866 Robalino J, Browdy CL, Prior S, Metz A, Parnell P, Gross P, Warr G (2004) Induction of antiviral immunity by double-stranded RNA in a marine invertebrate. J Virol 78(19): 10442-10448 Ruppert JM, Kinzler KW, Wong AJ, Bigner SH, Kao FT, Law ML, Seuanez HN, O'Brien SJ, Vogelstein B (1988) The GLI-Kruppel family of human genes. Mol Cell Biol 8(8): 3104-3113 Sarathi M, Simon MC, Ahmed VP, Kumar SR, Hameed AS (2008a) Silencing VP28 gene of white spot syndrome virus of shrimp by bacterially expressed dsRNA. Mar Biotechnol (NY) 10(2): 198-206 Sarathi M, Simon MC, Venkatesan C, Hameed AS (2008b) Oral administration of bacterially expressed VP28dsRNA to protect Penaeus monodon from white spot syndrome virus. Mar Biotechnol (NY) 10(3): 242-249 Sato H, Takimoto T, Tanaka S, Tanaka J, Raab-Traub N (1990) Concatameric replication of Epstein-Barr virus: structure of the termini in virus-producer and newly transformed cell lines. J Virol 64(11): 5295-5300 Schuh R, Aicher W, Gaul U, Cote S, Preiss A, Maier D, Seifert E, Nauber U, Schroder C, Kemler R (1986) A conserved family of nuclear proteins containing structural elements of the finger protein encoded by Kruppel, a Drosophila segmentation gene. Cell 47(6): 1025-1032 Sengupta T, Chen K, Milot E, Bieker JJ (2008) Acetylation of EKLF is essential for epigenetic modification and transcriptional activation of the beta-globin locus. Mol Cell Biol 28(20): 6160-6170 Shields JM, Yang VW (1997) Two potent nuclear localization signals in the gut-enriched Kruppel-like factor define a subfamily of closely related Kruppel proteins. J Biol Chem 272(29): 18504-18507 Shindo T, Manabe I, Fukushima Y, Tobe K, Aizawa K, Miyamoto S, Kawai-Kowase K, Moriyama N, Imai Y, Kawakami H, Nishimatsu H, Ishikawa T, Suzuki T, Morita H, Maemura K, Sata M, Hirata Y, Komukai M, Kagechika H, Kadowaki T, Kurabayashi M, Nagai R (2002) Kruppel-like zinc-finger transcription factor KLF5/BTEB2 is a target for angiotensin II signaling and an essential regulator of cardiovascular remodeling. Nat Med 8(8): 856-863 Soto MA, Shervette VR, Lotz JM (2001) Transmission of white spot syndrome virus (WSSV) to Litopenaeus vannamei from infected cephalothorax, abdomen, or whole shrimp cadaver. Dis Aquat Organ 45(2): 81-87 Speck SH, Chatila T, Flemington E (1997) Reactivation of Epstein-Barr virus: regulation and function of the BZLF1 gene. Trends Microbiol 5(10): 399-405 Subramaniam M, Gorny G, Johnsen SA, Monroe DG, Evans GL, Fraser DG, Rickard DJ, Rasmussen K, van Deursen JM, Turner RT, Oursler MJ, Spelsberg TC (2005) TIEG1 null mouse-derived osteoblasts are defective in mineralization and in support of osteoclast differentiation in vitro. Mol Cell Biol 25(3): 1191-1199 Sun CC, Thorley-Lawson DA (2007) Plasma cell-specific transcription factor XBP-1s binds to and transactivates the Epstein-Barr virus BZLF1 promoter. J Virol 81(24): 13566-13577 Suske G (1999) The Sp-family of transcription factors. Gene 238(2): 291-300 Suske G, Bruford E, Philipsen S (2005) Mammalian SP/KLF transcription factors: bring in the family. Genomics 85(5): 551-556 Turner J, Crossley M (1998) Cloning and characterization of mCtBP2, a co-repressor that associates with basic Kruppel-like factor and other mammalian transcriptional regulators. EMBO J 17(17): 5129-5140 Vanhecke D, Janitz M (2005) Functional genomics using high-throughput RNA interference. Drug Discov Today 10(3): 205-212 Wang YG, Hassan MD, Shariff M, Zamri SM, Chen X (1999) Histopathology and cytopathology of white spot syndrome virus (WSSV) in cultured Penaeus monodon from peninsular Malaysia with emphasis on pathogenesis and the mechanism of white spot formation. Dis Aquat Organ 39(1): 1-11 Wang Z, Hu L, Yi G, Xu H, Qi Y, Yao L (2004) ORF390 of white spot syndrome virus genome is identified as a novel anti-apoptosis gene. Biochem Biophys Res Commun 325(3): 899-907 Westenberg M, Heinhuis B, Zuidema D, Vlak JM (2005) siRNA injection induces sequence-independent protection in Penaeus monodon against white spot syndrome virus. Virus Res 114(1-2): 133-139 Wu FY, Wang SE, Chen H, Wang L, Hayward SD, Hayward GS (2004) CCAAT/enhancer binding protein alpha binds to the Epstein-Barr virus (EBV) ZTA protein through oligomeric interactions and contributes to cooperative transcriptional activation of the ZTA promoter through direct binding to the ZII and ZIIIB motifs during induction of the EBV lytic cycle. J Virol 78(9): 4847-4865 Yu X, McCarthy PJ, Lim HJ, Iempridee T, Kraus RJ, Gorlen DA, Mertz JE (2011) The ZIIR element of the Epstein-Barr virus BZLF1 promoter plays a central role in establishment and maintenance of viral latency. J Virol 85(10): 5081-5090 Zalani S, Coppage A, Holley-Guthrie E, Kenney S (1997) The cellular YY1 transcription factor binds a cis-acting, negatively regulating element in the Epstein-Barr virus BRLF1 promoter. J Virol 71(4): 3268-3274 Zalani S, Holley-Guthrie E, Kenney S (1995) The Zif268 cellular transcription factor activates expression of the Epstein-Barr virus immediate-early BRLF1 promoter. J Virol 69(6): 3816-3823 Zalani S, Holley-Guthrie EA, Gutsch DE, Kenney SC (1992) The Epstein-Barr virus immediate-early promoter BRLF1 can be activated by the cellular Sp1 transcription factor. J Virol 66(12): 7282-7292 Zhan WB, Wang YH, Fryer JL, Yu KK, Fukuda H, Meng QX (1998) white spot syndrome virus infection of cultured shrimp in China. J Aquat Anim Health 10: 405-410 Zhang JS, Moncrieffe MC, Kaczynski J, Ellenrieder V, Prendergast FG, Urrutia R (2001) A conserved alpha-helical motif mediates the interaction of Sp1-like transcriptional repressors with the corepressor mSin3A. Mol Cell Biol 21(15): 5041-5049 Zhang W, Bieker JJ (1998) Acetylation and modulation of erythroid Kruppel-like factor (EKLF) activity by interaction with histone acetyltransferases. Proc Natl Acad Sci U S A 95(17): 9855-9860 Zhu F, Zhang X Protection of Shrimp against White Spot Syndrome Virus (WSSV) with beta-1,3-D: -glucan-encapsulated vp28-siRNA Particles. Mar Biotechnol (NY) | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/43487 | - |
dc.description.abstract | Sp1-like proteins /Kruppel-like factors (Sp/KLF)轉錄因子家族,為相似度極高的鋅指蛋白質 (zinc finger protein),在細胞內調控多種轉錄活性。Sp/KLF轉錄因子於胺基酸序列C端皆具有三組Cys2-His2 (C2H2)鋅指元素 (zinc-finger DNA binding motif),並且會結合在GC-Box、CACCC-Box以及GT-Box序列上調節轉錄活性。本研究成功地自草蝦 (Penaeus monodon)選殖出Kruppel-like factor (PmKLF),PmKLF cDNA全長為1702 bps,並且可以轉譯出由360個胺基酸組成的蛋白質產物。胺基酸序列比對後發現,PmKLF和人類 (Homo sapiens)、小鼠(Mus musculus)、斑馬魚 (Danio rerio)以及水蚤的KLF11具有較高的相似度。此外,免疫螢光分析顯示,GFP-KLF融合蛋白質可於秋夜盜蛾細胞 (Spodoptera frugiperda, Sf9)細胞核中形成點狀聚集。冷光報導分析 (reporter assay)也指出 PmKLF促進白點症病毒(White spot syndrome virus, WSSV)啟動子ie1之轉錄活性。接著以電泳流動性轉移分析 (EMSA)發現PmKLF會結合於ie1啟動子GC- box序列形成蛋白質-DNA複合體。另外,若抑制白蝦PmKLF基因表現,能降低白點症病毒感染造成的高死亡率,因此推論PmKLF於白點症病毒感染時應扮演重要角色。而且當PmKLF表現受到抑制時,白點症病毒的複製數以及極早期基因ie1的表現量也會受到抑制,因此PmKLF可能藉由調節ie1基因表現影響白點症病毒的感染。 | zh_TW |
dc.description.abstract | Sp1-like proteins and Kruppel-like factors (Sp/KLF) are highly related zincfinger proteins that have crucial roles in transcription. The characteristic feature of this fami- ly is the presence of three zinc fingers in the C-terminal domain, which bind to GC- box, CACCC-box and GT-box sequence, to mediate transcription. This study reports the cloning and characteristics of a Kruppel-like factor (PmKLF) from shrimp, Penae- usmonodon (PmKLF). The full-length PmKLF cDNA is 1702 bps, encoding a poly- peptide of 360 amino acids. Sequence analysis revealed that the sequence of PmKLF is similar to that of KLF11 in humans, mice and zebrafish. Additionally, immunofluo- rescence analysis revealed that GFP-fusion KLF protein is located in the nucleus as dots in an insect cell line, Sf9. Reporter assay revealed that PmKLF enhances the ie1 promoter activity. Moreover, a specific protein-DNA complex binds to the GC-box sequences in the ie1 by electrophoresis mobility shift assays (EMSA). Knockdown of the expression of PmKLF transcript in WSSV-infected shrimp resulted in delayed cu- mulative mortalities, suggesting that PmKLF is important to WSSV infection. Additi- onally, inhibition of PmKLF expression reduced the copy number of WSSV and ie1 expression,revealing that PmKLF affects WSSV infection via interfering with ie1 expression. | en |
dc.description.provenance | Made available in DSpace on 2021-06-15T02:22:19Z (GMT). No. of bitstreams: 1 ntu-100-R97b47401-1.pdf: 1431809 bytes, checksum: 8e37472dff563284c27318b3fbe1b578 (MD5) Previous issue date: 2011 | en |
dc.description.tableofcontents | 目錄
中文摘要 i 英文摘要 ii 一、前言 1 二、材料和方法 9 1. 細胞株、實驗動物、草蝦組織以及白點症病毒液 9 2. 北方墨點分析 (Northern blotting) 9 3. 快速放大cDNA端點-草蝦KLF基因分析(Rapid amplification of cDNA ends, RACE) 10 4. 表現質體的建構 10 5. PmKLF於大腸桿菌 BL21 (DE3)之過量表現 11 6. PmKLF於Sf9昆蟲細胞之暫時性表現 11 7. 草蝦組織蛋白質萃取 11 8. 昆蟲細胞核蛋白質萃取 12 9. 西方點墨法 (Western blotting) 12 10. PmKLF影響白點症病毒ie1啟動子的活性分析 12 11. PmKLF於Sf9昆蟲細胞內之分布型態 13 12. 電泳游動性轉移分析 (Electrophoretic mobility shift assay, EMSA) 13 12. 雙股RNA製備 15 13. 白點症病毒感染、白蝦死亡率觀察以及病毒複製數檢測 15 14. 白蝦RNA萃取以及反轉錄反應(Reverse transcription) 16 三、結果 17 1. 草蝦Kruppel-like factor的選殖 17 2. 偵測草蝦KLF蛋白質以及草蝦KLF重組蛋白質之表現 18 3. 草蝦KLF蛋白質於細胞內之分布狀態 18 4. 草蝦KLF蛋白質調控白點症病毒ie1啟動子之活性 19 5. 草蝦KLF蛋白質以及電泳流動性轉移分析 19 6. 草蝦KLF蛋白質對白點症病毒感染以及蝦體死亡率的影響 20 四、討論 22 五、圖表 26 表1、本研究所使用之引子列表 26 圖1、草蝦KLF的序列以及分析。 27 圖2、以西方墨點分析偵測PmKLF的表現。 30 圖3、PmKLF於細胞內的分布型態觀察。 31 圖4、PmKLF對ie1啟動子轉錄活性的影響。 32 圖5、以電泳流動性轉移分析PmKLF和ie1啟動子的結合。 33 圖6、抑制白蝦KLF基因表現影響對白點症病毒感染造成之影響。 34 圖7、抑制白蝦KLF基因表現影響對白點症病毒ie1基因表現量之影響。 36 六、 參考文獻 37 | |
dc.language.iso | zh-TW | |
dc.title | 草蝦Kruppel-like factor 在白點症病毒感染時所扮演之角色 | zh_TW |
dc.title | Role of Penaeus monodon Kruppel-like factor (PmKLF)
in infection by white spot syndrome virus | en |
dc.type | Thesis | |
dc.date.schoolyear | 99-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 黃偉邦,呂健宏,王涵青 | |
dc.subject.keyword | Kruppel-like factors,GC-box,Penaeus monodon,WSSV,ie1, | zh_TW |
dc.relation.page | 48 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2011-08-17 | |
dc.contributor.author-college | 生命科學院 | zh_TW |
dc.contributor.author-dept | 生化科技學系 | zh_TW |
顯示於系所單位: | 生化科技學系 |
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