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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 鄭謙仁,龐飛 | |
dc.contributor.author | "Yu-Xing, Ding" | en |
dc.contributor.author | 丁宇星 | zh_TW |
dc.date.accessioned | 2021-06-15T01:23:23Z | - |
dc.date.available | 2009-12-25 | |
dc.date.copyright | 2009-08-13 | |
dc.date.issued | 2009 | |
dc.date.submitted | 2009-07-23 | |
dc.identifier.citation | Allan, G.M., Ellis, J.A., 2000, Porcine circoviruses: a review. J Vet Diagn Invest 12, 3-14.
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dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/42785 | - |
dc.description.abstract | 豬第二型環狀病毒 (porcine circovirus type 2;PCV2) 為造成豬隻離乳後多系統消耗性症候群 (postweaning multisystemic wasting syndrome ; PMWS) 之重要病原。近年研究顯示,感染PCV2的患豬淋巴組織會受到破壞,且同時影響細胞性免疫及體液性免疫系統表現。依據血清學調查結果,現場豬隻抗體陽性率接近100%,而抗體的產生及消長,除了反應免疫系統受到病毒刺激外,也可能控制著PCV2的複製並影響PMWS之疾病進展;特別是中和抗體 (neutralizing antibody;NA) 的表現,可能為左右PCV2病毒量之重要因素。於人工感染PCV2的實驗中發現,豬隻病毒血症的減緩,往往只在中和抗體血清陽轉之後發生;故學者認為中和抗體的缺乏和病毒複製的增加,兩者間緊密相關。然而,單純探討中和抗體和PCV2病毒量於現場豬隻的表現,卻不具統計上之負相關意義,中和抗體也無法將血清中PCV2病毒完全清除。因此,本實驗的目的為,藉由將豬隻飼養於一乾淨環境中,先單純探討中和抗體和PCV2病毒量變化之相關性;再於現場不同症狀的豬隻中,探討兩者關係之變遷,並期望能進一步了解體液性免疫和PCV2病毒量之相關性。實驗以兩階段進行;第一階段,選取6頭健康PCV2帶原豬隻,由5週齡開始持續採血至16週齡;第二階段,由雲林及屏東5個具有PMWS病史豬場,選取6-9週齡保育豬,共61頭,依據其臨床症狀分為三組 (healthy、mild與severe group);偵測兩階段所有豬隻PCV2抗體、中和抗體力價以及PCV2病毒量表現,進行中和抗體與PCV2病毒量相關性分析。實驗結果顯示,飼養於乾淨環境豬隻,其NA的發展,能與總PCV2抗體 (total PCV2 antibody;TA) 一致地變化,而有效降低PCV2病毒量,並達到完全清除病毒血症。於現場豬隻方面,可見PCV2普遍存在於6至9週齡保育豬,雖然豬隻有不等程度之臨床症狀,但皆可產生不同高低力價之PCV2抗體以及中和抗體。然而,NA和PCV2病毒量兩者之負相關僅存在於severe group豬隻。 | zh_TW |
dc.description.abstract | The phenomenon of impaired humoral immunity against PCV2 has been commonly noticed in postweaning multisystemic wasting syndrome (PMWS)-affected pigs. In previous research, PCV2 neutralizing antibody (NA) titer was inversely correlated with PCV2 load in experimentally inoculated pigs. However, the same correlation failed to be demonstrated in naturally PMWS-affected pigs. The aim of current study was to further investigate the humoral response against PCV2 via evaluating development and production of NA in pigs with different ages and clinical status. In first part of the study, 6 healthy field PCV2-carrier pigs were kept in laboratory animal house and studied in duration of 5 to 16 weeks of age. In second part of the study, 61 nursery pigs in 6-9 weeks of age from 5 different farms were categorized into three groups, namely healthy, mild, and severe animals based on clinical status. The determination of NA, total PCV2 antibodies (TA), and PCV2 load were comparatively studied as well as correlated with clinical status in all studied pigs. The results showed the presence of NA was able to diminish PCV2 load and completely eliminate viremia in 6 healthy field PCV2-carrier pigs. In field pigs, the highest NA and TA titer, and the lowest PCV2 load was present in the severe group. Additionally, the inverse correlation between PCV2 load and NA or TA titers only existed in pigs of severe group. In conclusion, the presence and development of NA can effectively diminish the PCV2 load with the synergistic aid of TA in some circumstances. | en |
dc.description.provenance | Made available in DSpace on 2021-06-15T01:23:23Z (GMT). No. of bitstreams: 1 ntu-98-R96629001-1.pdf: 658562 bytes, checksum: bf9c69c976daa18beda8b6c8d6ebb2e5 (MD5) Previous issue date: 2009 | en |
dc.description.tableofcontents | 目錄
摘要 I Abstract III 圖次 VIII 表次 IX 第一章 序言 1 第二章 文獻回顧 3 第一節 環狀病毒 3 1-1環狀病毒的病毒學分類 3 1-2豬環狀病毒結構及特性 3 1-3豬環狀病毒的感染及複製 4 第二節 豬隻離乳後多系統消耗性症候群 5 2-1 定義 5 2-2 歷史背景與流行 6 2-3 臨床症狀與病理變化 7 2-4 致病機制 8 2-4.1 病毒毒力相關因子 8 2-4.2 受感染豬隻標的細胞及組織內病毒分佈與複製 9 2-4.3 其他病原共同感染 11 第三節 豬隻免疫系統反應與病毒感染 12 3-1 免疫調節效應 12 3-2 體液性免疫之反應 13 3-2.1 母豬與PCV2移行抗體 14 3-2.2 PCV2總抗體變化與病毒之相互關係 15 3-2.3 PCV2特定抗體變化與病毒之相互關係 15 3-2.4 PCV2中和抗體變化與病毒之相互關係 16 第三章 材料與方法 18 第一節 實驗設計 18 1-1實驗設計流程圖 18 第二節 實驗材料 19 2-1 實驗動物及試驗豬場 19 2-2 豬第二型環狀病毒 19 2-3 豬腎細胞株 (PK-15 cell line) 20 2-3.1 細胞培養液 (DMEM-C) 20 2-3.2 細胞清洗液 (D-PBS) 20 2-3.3 D (+) Glucosamine, Hydrochloride 20 2-3.4 Hank’s Balanced Salt Solutions (HBSS) 21 第三節 實驗方法 21 3-1 樣本收集與保存 21 3-1.1 第一階段實驗採樣 21 3-1.2 第二階段實驗採樣 21 3-2 PCV2總抗體檢測 22 3-2.1 豬第二型環狀病毒抗原盤製作 22 3-2.2 免疫螢光抗體染色 (indirect immunofluorescence assay; IFA) 22 3-3 PCV2中和抗體試驗 (viral neutralization test) 23 3-4 同步定量聚合酶鏈鎖反應 (Quantitative real-time PCR reaction;qPCR) 24 3-5 統計分析 24 第四章 結果 28 第一節 PCV2健康帶原豬採樣結果 28 1-1 PCV2核酸量測定 28 1-2 PCV2總抗體力價 28 1-2.1 PCV2總抗體力價與PCV2核酸量之相互關係 28 1-3 PCV2中和抗體力價 29 1-3.1 PCV2中和抗體與PCV2核酸量之相互關係 29 1-4 PCV2總抗體力價與PCV2中和抗體力價之相互關係 29 第二節 現場豬隻採樣結果 31 2-1 PCV2核酸量測定 31 2-2 PCV2總抗體力價 31 2-2.1 PCV2總抗體力價與PCV2核酸量之相互關係 32 2-3 PCV2中和抗體力價 37 2-3.1 PCV2中和抗體力價與PCV2核酸量之相互關係 37 2-4 PCV2總抗體力價與PCV2中和抗體力價之相互關係 38 第五章 討論 44 第六章 參考文獻 54 附錄 64 | |
dc.language.iso | zh-TW | |
dc.title | 探討豬第二型環狀病毒中和抗體及病毒量於現場豬隻之相關性 | zh_TW |
dc.title | Correlation between the Neutralizing Antibodies against Porcine Circovirus Type 2 (PCV2) and the Viral Load in Conventional Pig Herds | en |
dc.type | Thesis | |
dc.date.schoolyear | 97-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 蕭世烜,張志成,劉涓 | |
dc.subject.keyword | 豬第二型環狀病毒,豬隻離乳後多系統消耗性症候群,中和抗體,體液性免疫,保育豬, | zh_TW |
dc.subject.keyword | PCV2,PMWS,NA,TA,nursery pigs, | en |
dc.relation.page | 72 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2009-07-24 | |
dc.contributor.author-college | 獸醫專業學院 | zh_TW |
dc.contributor.author-dept | 獸醫學研究所 | zh_TW |
顯示於系所單位: | 獸醫學系 |
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