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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 丁詩同 | |
| dc.contributor.author | Li-Ru Liu | en |
| dc.contributor.author | 劉利儒 | zh_TW |
| dc.date.accessioned | 2021-06-15T01:18:55Z | - |
| dc.date.available | 2009-10-01 | |
| dc.date.copyright | 2009-07-31 | |
| dc.date.issued | 2009 | |
| dc.date.submitted | 2009-07-27 | |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/42656 | - |
| dc.description.abstract | 血清澱粉狀蛋白A ( serum amyloid A, SAA) 不只是載脂蛋白，也是具有促進脂肪分解能力的脂肪細胞激素，但SAA促進脂肪分解作用的訊息傳遞路逕目前尚無研究，因此本文研究目的為研究SAA調控脂肪分解作用機制。在添加豬SAA重組蛋白和不同訊息傳遞抑制劑於豬脂肪細胞實驗中，發現SAA降低油滴包被蛋白(perilipin)、荷爾蒙敏感性解脂酶 (hormone sensitive lipase, HSL)及脂肪組織三酸甘油酯酶(adipose triacylglycerol lipase, ATGL) 的表現，且這些效應會被細胞外訊息調節蛋白激酶 (extracellular signal regulated kinase, ERK)及蛋白質激酶A (protein kinase A, PKA) 之抑制劑回復。SAA亦於猪脂肪細胞中增加ERK與PKA 之磷酸化，而且SAA增加脂肪分解的效應會被ERK與PKA之抑制劑中止。此外，SAA抑制過氧化體增生活化受體γ (peroxisome proliferator-activated receptor-gamma, PPARγ) mRNA表現之效應亦受ERK抑制劑回復。 為釐清PPARγ是否參與SAA經由活化ERK降低perilipin表現之訊息路徑，於添加SAA和不同抑制劑在已轉染猪perilipin啟動子之3T3L1脂肪細胞實驗中，發現SAA降低perilipin啟動子活性主要序列位置在PPARγ反應子 (peroxisome proliferator-activated receptor response element, PPRE) ，而且ERK也參與其中。綜合以上，證明SAA藉由ERK/PPARγ 與 PKA 訊息傳遞路徑降低perilipin表現，使三酸甘油酯分解成游離脂肪酸及甘油釋出脂肪細胞，達脂肪分解目的。 | zh_TW |
| dc.description.abstract | Serum amyloid A protein (SAA) is not only an apolipoprotein, but also a member of adipokines with potentials to enhance lipolysis in human and porcine adipocytes. The purpose of this study was to explore how SAA facilitates lipolysis in porcine adipocytes. Several predict signal transducer inhibitors and porcine SAA recombinant protein were used to treat porcine adipocytes in primary cell culture. We found SAA decreased the expression of perilipin, hormone sensitive lipase (HSL), and adipose triacylglycerol lipase (ATGL) mRNA and such effect could be recovered by extracellular signal-regulated kinase (ERK) and/or protein kinase A (PKA) inhibitors. The SAA also activates ERK and PKA via phosphorylating these kinases in porcine adipocytes. Moreover, SAA increased porcine adipocyte glycerol release, which was abrogated by ERK (PD98059) and PKA (H89) inhibitor, suggesting that ERK and PKA involve in mediating the SAA enhanced lipolysis. On the other hand, SAA down-regulated the expression of peroxisome proliferator-activated receptors gamma (PPARγ) mRNA which was recovered by ERK inhibitor. To understand whether PPARγ participates in SAA promoted lipolysis via ERK, we performed the porcine perilipin promoter assay with luciferase as promoter in differentiated 3T3L1 adipocytes. We found that SAA reduced porcine perilipin promoter specifically through the function of its PPARγ response element (PPRE), and this effect was recovered by ERK inhibitor. However, PKA did not have a role in the SAA regulated PPRE activity. These findings demonstrate that SAA induced lipolysis is a result of down-regulation of perilipin via ERK/PPARγ and PKA signaling pathways which are novel pathways by which SAA directly stimulates lipolysis to liberate glycerol efflux from adipocytes. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-15T01:18:55Z (GMT). No. of bitstreams: 1 ntu-98-R96626010-1.pdf: 1228331 bytes, checksum: 9ddbe677d2e4c410429e7dfb6e659a32 (MD5) Previous issue date: 2009 | en |
| dc.description.tableofcontents | 口試委員會審定書--------------------------------------------------------------------------------- i
中文摘要---------------------------------------------------------------------------------------------ii 英文摘要--------------------------------------------------------------------------------------------iii 文獻檢討------------------------------------------------------------------------- -------------------1 一、 前言---------------------------------------------------------- -------------------1 二、 脂肪分解作用-------------------------------------------- -------------------1 三、 血清澱粉狀蛋白A------------------------------------------------------------8 四、 實驗目的-----------------------------------------------------------------------9 Materials and methods---------------------------------------------------------------- -----------10 Results----------------------------------------------------------------------------------------------18 MEK1/2 inhibitor blocks SAA mediated down-regulation of perilipin protein in porcine adipocytes SAA activates ERK1/2 --------------------------------------------18 SAA activated ERK1/2----------------------------------------------------------------------20 The MEK1/2 inhibitor blocks SAA induced lipolysis in porcine adipocytes ---------21 The MEK1/2 inhibitor enhances SAA mediated downregulation of PPARγ and HSL mRNA in porcine adipocytes-----------------------------------------------------------------22 SAA decreased perilipin expression by the PPRE on perilipin gene through ERK pathway------------------------------------------------------------------------------------------24 PKA inhibitor block SAA mediated down-regulation of perilipin protein in porcine adipocytes---------------------------------------------------------------------------------------26 SAA activated PKA activity -----------------------------------------------------------------27 The PKA inhibitor, H89, blocks SAA induced lipolysis in porcine adipocytes-------28 Effect of PKA on SAA regulated PPARγ, HSL and ATGL expression----------------29 PKA inhibitor did not affect the SAA decreased PPRE of perilipin promoter activity-------------------------------------------------------------------------------------------31 Discussion------------------------------------------------------------------------------------------32 Reference-------------------------------------------------------------------------------------------37 Appendix--------------------------------------------------------------------------------------------41 | |
| dc.language.iso | en | |
| dc.subject | PKA | zh_TW |
| dc.subject | SAA | zh_TW |
| dc.subject | 脂肪分解作用 | zh_TW |
| dc.subject | perilipin | zh_TW |
| dc.subject | HSL | zh_TW |
| dc.subject | ERK | zh_TW |
| dc.subject | PPARγ | zh_TW |
| dc.subject | SAA | en |
| dc.subject | PKA | en |
| dc.subject | PPARγ | en |
| dc.subject | ERK | en |
| dc.subject | HSL | en |
| dc.subject | perilipin | en |
| dc.subject | lipolysis | en |
| dc.title | 血清澱粉狀蛋白A增進脂肪分解作用之訊息傳遞路徑 | zh_TW |
| dc.title | Serum amyloid A induced lipolysis signal transduction pathway | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 97-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.coadvisor | 林劭品 | |
| dc.contributor.oralexamcommittee | 歐柏榮,陳洵一 | |
| dc.subject.keyword | SAA,脂肪分解作用,perilipin,HSL,ERK,PPARγ,PKA, | zh_TW |
| dc.subject.keyword | SAA,lipolysis,perilipin,HSL,ERK,PPARγ,PKA, | en |
| dc.relation.page | 41 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2009-07-27 | |
| dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
| dc.contributor.author-dept | 動物科學技術學研究所 | zh_TW |
| 顯示於系所單位： | 動物科學技術學系 | |
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