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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 林順福 | |
dc.contributor.author | Ya-Ping Lin | en |
dc.contributor.author | 林亞平 | zh_TW |
dc.date.accessioned | 2021-06-15T01:18:44Z | - |
dc.date.available | 2014-07-28 | |
dc.date.copyright | 2009-07-28 | |
dc.date.issued | 2009 | |
dc.date.submitted | 2009-07-27 | |
dc.identifier.citation | Álvarez, I., and J. F. Wendel. 2003. Ribosomal ITS sequences and plant phylogenetic inference. Molecular Phylogenetics and Evolution 29: 417-434.
Balasaravanan, T., P. K. Pius, R. R. Kumar, N. Muraleedharan, and A. K. Shasany. 2003. Genetic diversity among south Indian tea germplasm (Camellia sinensis, C. assamica and C. assamica spp. lasiocalyx) using AFLP markers. Plant Science 165: 365-372. Chase, M. W., N. Salamin, M. Wilkinson, J. M. Dunwell, R. P. Kesanakurthi, N. Haidar, and V. Savolainen. 2005. Land plants and DNA barcodes: short-term and long-term goals. Philosophical Transactions of the Royal Society B-Biological Sciences 360: 1889-1895. Chen, L., Q. K. Gao, D. M. Chen, and C. J. Xu. 2005. The use of RAPD markers for detecting genetic diversity, relationship and molecular identification of Chinese elite tea genetic resources [Camellia sinensis (L.) O. Kuntze] preserved in a tea germplasm repository. Biodiversity and Conservation 14: 1433-1444. Chen, L., and S. Yamaguchi. 2005. RAPD markers for discriminating tea germplasms at the inter-specific level in China. Plant Breeding 124: 404-409. Chiu, C. F., J. C. Lin, and J. J. Huang. 2006. The 70th Anniversary Memorial. Tea Research and Extension Station. Taiwan. Cho, Y., J. P. Mower, Y. L. Qiu, and J. D. Palmer. 2004. Mitochondrial substitution rates are extraordinarily elevated and variable in a genus of flowering plants. PNAS 101: 17741-17746. Demesure, B., N. Sodzi, and R. J. Petit. 1995. A set of universal primers for amplification of polymorphic noncoding regions of mitochondrial and chloroplast DNA in plants. Molecular Ecology 4: 129-131. Denda, T., K. Kosuge, K. Watanabe, M. Ito, Y. Suzuki, P. S. Short, and T. Yahara. 1995. Intron length variation if the Adh gene in Brachyscome (Asteraceae). Plant Molecular Biology 28: 1067-1073. Devarumath, R. M., S. Nandy, V. Rani, S. Marimuthu, N. Muraleedharan, and S. N. Raina. 2002. RAPD, ISSR and RFLP fingerprints as useful markers to evaluate genetic integrity of micropropagated plants of three diploid and triploid elite tea clones representing Camellia sinensis (China type) and C. assamica ssp assamica (Assam-India type). Plant Cell Reports 21: 166-173. Doyle, J. J., and J. L. Doyle. 1990. Isolation of plant DNA from fresh tissue. Focue 12: 13-15. Dumolin-Lapegue, S., M. H. Pemonge, and R. J. Petit. 1997. An enlarged set of consensus primers for the study of organelle DNA in plants. Molecular Ecology 6: 393-7. Esselman, E. J., L. Jianqiang, D. J. Crawford, J. L. Windus, and A. D. Wolfe. 1999. Clonal diversity in the rare Calamagrostis porteri ssp. insperata (Poaceae): comparative results for allozymes and random amplified polymorphic DNA (RAPD) and intersimple sequence repeat (ISSR) markers. Molecular Ecology 8: 443-451. Hu, C. Y. 2004. Studies on the Variations in Leaf Characters and DNA Sequences of Tea Germplasm in Taiwan. Master Thesis, Graduate Institute of Agronomy, National Taiwan University. Hung, C. Y., K. H. Wang, C. C. Huang, X. Gong, X. J. Ge, and T. Y. Chiang. 2008. Isolation and characterization of 11 microsatellite loci from Camellia sinensis in Taiwan using PCR-based isolation of microsatellite arrays (PIMA). Conservation Genetics 9: 779-781. Katoh, S., M. Katoh, Y. Takeda, and M. Omori. 2003. Genetic diversity within cultivated teas based on nucleotide sequence comparison of ribosomal RNA maturase in chloroplast DNA. Euphytica 134 (3): 287-295. Kaundun, S. S., A. Zhyvoloup, and Y. G. Park. 2000. Evaluation of the genetic diversity among elite tea (Camellia sinensis var. sinensis) accessions using RAPD markers. Euphytica 115 (1): 7-16. Kaundun, S. S., and S. Matsumoto. 2002. Heterologous nuclear and chloroplast microsatellite amplification and variation in tea, Camellia sinensis. Genome 45: 1041-1048. Kaundun, S. S., and S. Matsumoto. 2003. Development of CAPS markers based on three key genes of the phenylpropanoid pathway in Tea, Camellia sinensis (L.) O. Kuntze, and differentiation between assamica and sinensis varieties. Theoretical and Applied Genetics 106: 375-383. Kaundun, S. S., and S. Matsumoto. 2004. PCR-based amplicon length polymorphisms (ALPs) at microsatellite loci and indels from non-coding DNA regions of cloned genes as a means of authenticating commercial Japanese green teas. Journal of the Science of Food and Agriculture 84: 895-902. Kieleczawa, J. 2006. Fundamentals of sequencing of difficult templates-an overview. Journal of Biomolecular Techniques 17: 207-17. Kieleczawa, J. 2009. DNA sequencing III: dealing with difficult templates. pp. 54-58. Jones and Bartlett Publishers. Sudbury. Lai, J. A., W. C. Yang, and J. Y. Hsiao. 2001. An assessment of genetic relationships in cultivated tea clones and native wild tea in Taiwan using RAPD and ISSR maskers. Botanical Bulletin of Academia Sinica 42: 93-100. Li, T. C. and C. G. Jhang. 2003. Tea Germplasm Resources In Taiwan. Tea Research and Extension Station. Taiwan. Lin, S. Y. 2002. Detection of Genetic Relationship in Tea (Camellia sinensis (L.) O. Kuntze) With RAPD markers. Master Thesis, Graduate Institute of Horticulture, National Taiwan University. Magoma, G. N., F. N. Wachira, M. Obanda, M. Imbuga, and S. G. Agong. 2000. The use of catechins as biochemical markers in diversity studies of tea (Camellia sinensis). Genetic Resources and Crop Evolution 47: 107-114. Matsumoto, S., A. Takeuchi, M. Hayatsu, and S. Kondo. 1994. Molecular cloning of phenylalanine ammonia-lyase cDNA and classification of varieties and cultivars of tea plants (Camellia sinensis) using the tea PAL cDNA probe. Theoretical and Applied Genetics 89: 671-675. Mayer, M. S., and S. K. Bagga. 2002. The phylogeny of Lens (Leguminosae): new insight from ITS sequence analysis. Plant Systematics and Evolution 232: 145-154. Mondal, T. K. 2002. Assessment of genetic diversity of tea (Camellia sinensis (L.) O. Kuntze) by inter-simple sequence repeat polymerase chain reaction. Euphytica 128: 307-315. Mower, J. P., P. Touzet, J. S. Gummow, L. F. Delph, and J. D. Palmer. 2007. Extensive variation in synonymous substitution rates in mitochondrial genes of seed plants. BMC Evolutionary Biology 7: 135. Palmer, J. D., K. L. Adams, Y. R. Cho, C. L. Parkinson, Y. L. Qiu, and K. M. Song. 2000. Dynamic evolution of plant mitochondrial genomes: mobile genes and introns and highly variable mutation rates. Proceedings of the National Academy of Sciences of the United States of America 97: 6960-6966. Paul, S., F. N. Wachira, W. Powell, and R. Waugh. 1997. Diversity and genetic differentiation among populations of Indian and Kenyan tea (Camellia sinensis (L) O Kuntze) revealed by AFLP markers. Theoretical and Applied Genetics 94: 255-263. Prince, L. M., and C. Parks. 2001. Phylogenetic relationships of Theaceae inferred from chloroplast DNA sequence data. American Journal of Botany 88 (12): 2309-2320. Ravi, V., J. P. Khurana, A. K. Tyagi, and P. Khurana. 2008. An update on chloroplast genomes. Plant Systematics and Evolution 271: 101-122. Sang, T., M. J. Donoghue, and D. M. Zhang. 1997. Evolution of alcohol dehydrogenase genes in peonies (Paeonia): phylogenetic relationships of putative nonhybrid species. Molecular Biology and Evolution 14: 994-1007. Singh, D., and P. S. Ahuja. 2006. 5S rDNA gene diversity in tea (Camellia sinensis (L.) O. Kuntze) and its use for variety identification. Genome 49: 91-96. Small, R. L., J. A. Ryburn, R. C. Cronn, T. Seelanan, and J. F. Wendel. 1998. The tortoise and the hare: choosing between noncoding plastome and nuclear ADH sequences for phylogeny reconstruction in a recently diverged plant group. American Journal of Botany 85: 1301-1315. Soltis, D. E., and P. S. Soltis. 2001. Choosing an Approach and an Appropriate Gene for Phylogenetic Analysis. In: Molecular Systematics of Plants II: DNA Sequencing. pp. 36-49. Kluwer Academics Publishers. New York. Taberlet, P., L. Gielly, G. Pautou, and J. Bouvet. 1991. Universal primers for amplification of 3 noncoding regions of chloroplast DNA. Plant Molecular Biology 17: 1105-1109. Tanaka, J. 1996. RAPD linkage map of tea plant and the possibility of applicationin tea genetics and breeding. Tea Research Journal 8444-45. Tang, S. Q., X. Y. Bin, L. Wang, and Y. Zhong. 2006. Genetic diversity and population structure of yellow camellia (Camellia nitidissima) China as revealed by RAPD and AFLP markers. Biochemical Genetics 44:9-10. Thomas, J., D. Vijayan, S. D. Joshi, S. J. Lopez, and R. R. Kumar. 2006. Genetic integrity of somaclonal variants in tea (Camellia sinensis (L.) O Kuntze) as revealed by inter simple sequence repeats. Journal of Biotechnology 123: 149-154. Tsai, I. C. 2003. The Studies of the Genetic Diversity of the Gerplasm of Camellia sinensis in Taiwan and the Development of DNA Fingerprinting for Taiwan Tea Cultivars. Master Thesis, Graduate Institute of Agronomy, National Taiwan University. Tseng, Y. H. 2007. Study on DNA Sequence Variations in Adh Gene of Legume Species. Master Thesis, Graduate Institute of Agronomy, National Taiwan University. Vijayan, K. and C. H. Tsou. 2008. Technical report on the molecular phylogeny of Camellia with nrITS: the need for high quality DNA and PCR amplification with Pfu-DNA polymerase. Botanical Studies 49: 177-188. Wachira, F. N., W. Powell, and R. Waugh. 1997. An assessment of genetic diversity among Camellia sinensis L. (cultivated tea) and its wild relatives based on randomly amplified polymorphic DNA and organelle specific STS. Heredity 78: 603-611. Yang, J. B., H. T. Li, S. X. Yang, D. Z. Li, and Y. Y. Yang. 2006. The application of four DNA sequences to studying molecular phylogeny of Camellia (Theaceae). Acta Botanica Yunnanica 28 (2): 108-114. Zhao, L. P., Z. Liu, L. Chen, M. Z. Yao, and X. C. Wang. 2008. Generation and characterization of 24 novel EST derived microsatellites from tea plant (Camellia sinensis) and cross-species amplification in its closely related species and varieties. Conservation Genetics 9: 1327-1331. Zietkiewicz, E., A. Rafalski, and D. Labuda. 1994. Genome fingerprinting by simple sequence repeat (SSR)-anchored polymerase chain reaction amplification. Genomics 20: 176-183. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/42649 | - |
dc.description.abstract | 茶為我國重要之經濟作物,為確保國內比賽茶品種純正及落實生產履歷政策,開發快速且穩定之品種鑑定方法實為必要。本研究篩選出7條ISSR引子及1條功能性基因引子可產生12個核心標誌及6個輔助標誌做為國內重要茶栽培種之DNA指紋,此工具可應用於檢測國內比賽茶及市售茶葉。利用ISSR DNA分子標誌,本研究所檢測比賽茶之烏龍茶及東方美人茶均發現部分之未知品種名稱樣品。此外為提升檢測效率,將不同引子混合進行PCR分析,結果均不能獲得穩定條帶;但將不同引子產生之PCR產物混合進行電泳分析,則可偵測預期之分子標誌,便可提昇檢測效率。
本研究由4個細胞核及8個細胞質(含4個葉綠體及4個粒線體)序列變異較大之片段篩選出具較大序列變異性之ITS2及M8片段,目前開發出ITS2序列與粒線體部分序列,其中品種間ITS2具有33個單一核苷酸多型性,而品種間之M8具有5個核苷酸缺失/插入及2個單一核苷酸多型性。此外分析不同品種之定序訊號結果,發現M8的定序訊號可偵測到不同比例的品種混合(50%、40%、30%、20%及10%)。為了將DNA條碼應用於成茶的品種鑑定,故將引子設計成可擴增小片段DNA的引子,如此定序一次即可得到所要分析的片段。本研究推薦以ITS2及M8作為茶葉商品之DNA條碼,以DNA條碼驗證比賽茶中有疑問之樣本,發現東方美人茶確實有品種混合的樣本。 本研究顯示ISSR分子標誌可應用於成茶的品種鑑定,但它缺乏可偵測品種混合的敏感度,而另一方面,DNA條碼可偵測品種混合,故我們建議DNA條碼可應用在商業茶葉的產銷履歷上,而ISSR分子標誌則可輔助DNA條碼重複確認樣本之品種鑑定。 | zh_TW |
dc.description.abstract | Tea is one of the most important economic crops in Taiwan. To ensure the authenticity of competition teas and to achieve traceability policy, an efficient method of variety identification for processed teas is urgently expected. In this study, twelve core markers and six auxiliary markers generated from seven ISSR primers and one functional gene were recommended to fingerprint prevailing tea varieties grown in Taiwan. The core markers are also suggested to examine the varieties of competition teas and commercial teas. According to ISSR DNA markers, some unknown varieties were found in the competition tea samples of Oolong tea and oriental Beauty tea. To enhance the efficiency of variety identification, two ISSR primers were randomly selected and mixed for PCR amplification. However, the unstable band patterns were found in many attempts. But stable markers could be obtained from electrophoresis with PCR products.
ITS2 (nuclear) and M8 (cytoplasmic) DNA sequences with large variations were screened out of twelve DNA fragments (four nuclear, four chloroplastic and four mitochondrial) to identify tea varieties in Taiwan. Among varieties of tea, thirty-three SNPs were found in the ITS2 fragments, and five InDels and two SNPs were found in the M8 fragments. DNA mixtures of different varieties with various ratios (50%, 40%, 30%, 20%, and 10%) were identified based on sequencing signals of SNPs or InDels of M8 fragments. In order to apply DNA barcodes in variety identification of made teas, the primers were designed to amplify short fragments within the length limitation of one confident sequencing. The ITS2 and M8 sequences were recommended as DNA barcodes for commercial teas. Mixture of different varieties that was unable detected by ISSR DNA markers was verified by ITS2 and M8 DNA barcodes. Our studies have demonstrated that ISSR DNA markers are useful in variety identification of teas. However, they lack in sensitivity to detect variety mixtures. On the other hand, DNA sequence barcodes could detect variety mixtures. In this study, DNA sequence barcodes are suggested to be applied in labeling, tracing or primarily detecting commercial tea products, and ISSR DNA markers are prepared for reconfirmation of variety identification. | en |
dc.description.provenance | Made available in DSpace on 2021-06-15T01:18:44Z (GMT). No. of bitstreams: 1 ntu-98-R96621108-1.pdf: 1070380 bytes, checksum: 71009fae59b0c4bc7524a9140df03751 (MD5) Previous issue date: 2009 | en |
dc.description.tableofcontents | 中文摘要 1
SUMMARY 2 INTRODUCTION 4 PREVIOUS STUDIES 7 Genetic markers of Camellia sinensis 7 Functional genes of Camellia sinensis 9 Organelle genomes of Camellia sinensis 9 MATERIALS AND METHODS 11 Materials 11 Varieties utilized for DNA fingerprinting analysis 11 Commercial samples for variety identification 11 Varieties utilized for DNA barcode construction 11 Methods 16 Molecular identification technologies of made tea products 16 Development of DNA fingerprinting 16 Development of DNA barcode 16 Multi-PCR evaluation 19 Organelle genome sequencing of mixing samples 19 DNA isolation 19 Polymerase chain reaction 20 Electrophoresis 20 Sequencing 21 Data analysis 21 RESULTS 22 Development of DNA fingerprints 22 Variety identification of competition teas 22 Variety identification of commercial product of made tea 23 Multi-PCR evaluation 27 PCR with mixed primers 27 Electrophoresis with mixed up PCR products 28 Development of DNA barcode system 29 Organelle DNA sequencing of mixing samples 34 DISCUSSION 37 The application of ISSR markers in variety identification of made tea products 37 The potential of DNA barcodes for variety identification of made tea products 38 Molecular markers applied in made teas 40 REFERENCE 41 | |
dc.language.iso | en | |
dc.title | ISSR分子標誌與DNA條碼應用於茶葉之品種鑑定 | zh_TW |
dc.title | The Applications of ISSR Markers and DNA Barcodes in Variety Identification of Tea | en |
dc.type | Thesis | |
dc.date.schoolyear | 97-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 盧煌勝,葉茂生,蔡右任,邱垂豐 | |
dc.subject.keyword | DNA條碼,ISSR分子標誌,成茶,品種鑑定,品種混合, | zh_TW |
dc.subject.keyword | DNA barcode,ISSR marker,made tea,variety identification,variety mixture, | en |
dc.relation.page | 45 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2009-07-27 | |
dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
dc.contributor.author-dept | 農藝學研究所 | zh_TW |
顯示於系所單位: | 農藝學系 |
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