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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 鄭謙仁(Chian-Ren Jeng) | |
| dc.contributor.author | Te-Yu Chan | en |
| dc.contributor.author | 詹德裕 | zh_TW |
| dc.date.accessioned | 2021-06-15T01:17:01Z | - |
| dc.date.available | 2013-08-22 | |
| dc.date.copyright | 2011-08-22 | |
| dc.date.issued | 2011 | |
| dc.date.submitted | 2011-08-16 | |
| dc.identifier.citation | Adekambi, T., Foucault, C., La Scola, B., Drancourt, M., 2006, Report of two fatal cases
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/42587 | - |
| dc.description.abstract | 皮內結核菌素檢測為台灣牛型結核病的官方檢驗方法。皮內結核菌素檢測是一種細胞免疫反應，過程中動物體需要接受兩次的保定方能完成此檢驗，對於馴化非良好的動物如鹿及野生動物有因緊迫造成損失的疑慮。此外，重複施行皮內結核菌素試驗必須間隔三個月，此等待期成為控制及撲滅疾病的重大缺點。使用血液樣本執行酵素聯結免疫吸附法或是聚合酶鏈鎖反應，也許可以彌補皮內結核菌素檢驗的缺點。本實驗中，從牛隻與鹿隻收集血液，進行聚合酶鏈鎖反應與酵素聯結免疫吸附法，血液檢測結果與培養、病理學及組織聚合酶鏈鎖反應的結果互相比較。若以培養結果當作金標準，147頭皮內結核菌素檢測陽性牛，其周邊血液單核細胞聚合酶鏈鎖反應敏感性為7.7% (2/26)，特異性為95.0% (115/121)；酵素連結免疫吸附法敏感性為0% (0/26)，特異性為95.0% (115/121)，可知牛結核病血液檢測具低敏感性及高特異性的特徵。在相同條件下，20頭鹿隻的周邊血液單核細胞聚合酶鏈鎖反應，其結果顯示此法不具敏感性(0%，0/7)但有高特異性(100%，7/7)；酵素聯結免疫吸附法則有高敏感性(85.7%，6/7)及中等特異性(50%，5/10)，且ELISA與皮內結核菌素試驗與培養結果的相關係數皆為0.647(屬於中等吻合度)，且血液試驗是實施一次保定即可採集及檢體的方式，不若結核菌素需要兩次保定，可避免馴化非良好的鹿隻因強大持續緊迫造成損失。因此，血液試驗在乳牛清除結核病有敏感性過低的疑慮，然而對於檢測鹿隻牛型結核病感染，血液試驗具有清楚的效益。 | zh_TW |
| dc.description.abstract | Intradermal tuberculin test (ITT) is the official test for diagnosis of bovine tuberculosis in Taiwan. ITT is a cell-mediated immune response that acquires two times of restraint and handling the animal to complete the examination, and safety issue is always concerned in non-domestic animal like deer or wildlife to perform this test. In addition, repeated or confirmed ITT test in the previous ITT positive animal should be performed in three months later which results in a significant drawback in the aspect of disease control and eradication. Using blood samples to perform enzyme-linked immunosorbent assays (ELISAs) or polymerase chain reaction (PCR) may provide an alternative assay to correct the shortcomings of ITT. In this study, blood samples collected from cattle and deer were tested by PCR and ELISA, and the results were subsequently correlated with the results of pathology, microbiology and PCR in the tissue examined. Using culture results as the golden standard in 147 ITT positive cattle, the sensitivity of PCR in peripheral blood mononuclear cells (PBMC) was 7.7% (2/26) and the specificity was 95.0% (115/121), the sensitivity of the ELISA was 0% (0/26) and the specificity was 95.0% (115/121). Under the same conditions of 17 deer, the sensitivity of PCR in PBMC was 0% (0/7) and the specificity was 100% (10/10), the sensitivity of the ELISA was 85.7% (6/7) and the specificity was 50% (5/10). In deer, the correlation coefficient between ELISA and ITT was 0.647(substantial agreement), and the correlation coefficient between ELISA and culture was also 0.647 (substantial agreement). Therefore, the blood tests for Mycobacterium bovis infection in cattle maybe not so helpful; however, blood tests are effective to detect Mycobacterium bovis infection in deer. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-15T01:17:01Z (GMT). No. of bitstreams: 1 ntu-100-R98629012-1.pdf: 1561694 bytes, checksum: e7a8a9bedcfaa90fa43104fe918d5429 (MD5) Previous issue date: 2011 | en |
| dc.description.tableofcontents | 目錄
口試委員審定書....Ⅰ 致謝............Ⅱ 中文摘要........Ⅲ 英文摘要........Ⅳ 目錄...........Ⅴ 表目錄.........Ⅷ 圖目錄.........Ⅸ 附錄...........X 第一章、緒論…………………………………………………………………….……1 第二章、文獻探討…………………………………………………………………2 第一節 歷史背景與流行病學...............3 第二節 分枝桿菌的特徵..................3 一、 形態學與染色特性................3 二、 細菌的結構及其組成..............4 三、 分類學........................4 （一）Mycobacterium tuberculosis complex ...........5 （二）Nontuberculosis mycobacteria…………………………….…..…….6 四、 培養條件.........................................7 第三節 分枝桿菌的宿主範圍……………………………………………………..8 第四節 結核病的臨床症狀與肉眼顯微病變.................9 一、 臨床症狀….…………………………………………………………… …..9 二、 肉眼及顯微病變............10 第五節 皮內結核菌素試驗.........11 第六節 聚合酶鍊鎖反應分析法.....12 第七節 酵素連結免疫分析法試驗....13 第三章、材料與方法…………………………………………………………………17 第一節 實驗設計 17 第二節 樣本採集 19 第三節 病理學檢驗 19 第四節 細菌分離及培養 20 第五節 聚合酶鏈鎖反應 20 一、 周邊血液單核細胞的分離方式 20 二、 組織與周邊血液單核細胞萃取核酸 21 三、 DNA的定量與定性 21 四、 16s rRNA 23 五、 熱休克蛋白(Heat shock protein, HSP) 23 六、 牛型結核病(TbD1) 24 七、 人型結核病(RD8) 25 第六節 酵素聯結免疫吸附分析法的檢驗 27 一、 Mycobacterium bovis的抗體檢測 28 二、 Mycobacterium avium subsp. paratuberculosis的抗體檢測 25 第七節 統計分析 29 一、 敏感性及特異性 29 二、 相關係數 30 三、 T檢定 30 第四章、結果…………………………………………………………………………30 第一節 樣本的採集數量 30 第二節 牛型結核病檢測 31 一、結核菌培養結果 32 二、病理學檢驗 34 三、組織核酸聚合酶鏈鎖反應 35 第三節 血液檢測 37 一、周邊血液單核細胞核酸聚合酶鏈鎖反應 37 二、酵素免疫吸附分析法檢驗 38 （一）Mycobacterium bovis的抗體檢測 38 （二）Mycobacterium avium subsp. paratuberculosis的抗體 檢測………..38 第四節 統計分析 41 第五章、討論…………………………………………………………………………45 第一節 牛隻結果討論 45 一、皮內結核菌素陽性結果的討論 45 二、血液試驗結果的討論 48 （一）周邊血液單核細胞之TbD1 PCR檢測 48 （二）Mycobacterium bovis的抗體檢測 49 三、牛隻牛型結核病試驗總論 51 第二節 鹿隻相關討論 51 一、皮內結核菌素結果討論 52 二、血液試驗結果的討論 53 （一）周邊血液單核細胞之TbD1 PCR檢測 53 （二）Mycobacterium bovis的抗體檢測 54 三、鹿隻牛型結核病試驗總論 55 第六章、參考文獻……………………………………………………………………56 | |
| dc.language.iso | zh-TW | |
| dc.title | 利用血液檢體偵測台灣牛隻及鹿隻的牛型結核病 | zh_TW |
| dc.title | Diagnosis of Bovine Tuberculosis in Cattle and Deer through Blood Samples | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 99-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.coadvisor | 龐飛(Victor Fei Pang),蕭世烜(Shih-Hsuan Hsiao) | |
| dc.contributor.oralexamcommittee | 劉涓(Jiuan Judy Liu),張志成(Chih-Cheng Chang) | |
| dc.subject.keyword | 牛結核病,鹿結核病,聚合酶,鏈鎖反應,酵素連結免疫分析法, | zh_TW |
| dc.subject.keyword | bovine tuberculosis,cervidae tuberculosis,polymerase chain reaction,enzyme-linked immunosorbent assay, | en |
| dc.relation.page | 91 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2011-08-16 | |
| dc.contributor.author-college | 獸醫專業學院 | zh_TW |
| dc.contributor.author-dept | 獸醫學研究所 | zh_TW |
| 顯示於系所單位： | 獸醫學系 | |
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| 檔案 | 大小 | 格式 | |
|---|---|---|---|
| ntu-100-1.pdf 目前未授權公開取用 | 1.53 MB | Adobe PDF |
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