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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 李雨 | |
dc.contributor.author | Tak-Wai Fung | en |
dc.contributor.author | 馮德威 | zh_TW |
dc.date.accessioned | 2021-06-15T01:12:18Z | - |
dc.date.available | 2012-07-31 | |
dc.date.copyright | 2009-07-31 | |
dc.date.issued | 2009 | |
dc.date.submitted | 2009-07-30 | |
dc.identifier.citation | 1. U. Lei, Micro/Nano electromechanics lecture notes, Institute of Applied Mechanics, National Taiwan University, 2008.
2. U. Lei, C. W. Huang, James Chen, C. Y. Yang, Y. J. Lo, Andrew Wo, C. F. Chen and T. W. Fung, “A travelling wave dielectrophoretic pump for blood delivery”, Lab on a Chip., vol.9, pp.1349-1356,2009. 3. T. B. Jones, “Electromechanics of Particles”, Cambridge University Press, 1995. 4. H. A. Pohl, Dielectrophoresis, Cambridge (UK), Cambridge University Press, 1978. 5. J. S. Batchelder, “Dielectrophoretic manipulator,” Rev. Sci. Instrum., vol.54, pp.300-302, 1983. 6. S. Masuda, M. Washizu, and M. Iwadare, “Separation of Small Particles Suspended in Liquid by Nonuniform Traveling Field”, IEEE Trans. Ind. Appl. IN, vol. IA-23, pp. 474-480, 1987. 7. S. Masuda, M. Washizu, and I. Kawabata, “Movement of Blood Cells in Liquid by Nonuniform Traveling Field”, IEEE Trans. Ind. Appl. IN, vol.24, pp.217-222, 1988. 8. X. B. Wang, Y. Huang, R. Holzel, Burt, J. P. H. and R. Pethig, “Theoretical and experimental investigations of the interdependence of the dielectric, dielectrophoretic and electrorotational behaviour of colloidal particles”, J. Phys. D: Appl. Phys., vol. 26, pp.312-322, 1993. 9. G. Fuhr, R. Hagedorn, T. Muller, B. Wagner, and W. Benecke, “Linear motion of dielectric particles and living cells in microfabricated structures induced by traveling electric fields”, Proc. of IEEE MEMS, pp.259-264, 1991. 10. G. Fuhr, T. Muller , Th. Schnelle, R. Hagedorn, , A. Voigt, S. Fiedler, W. M. Arnold, U. Zimmermann, B. Wagner, and A. Heuberger, “Radio-Frequency Microtools for Particle and Live Cell Manipulation”, Naturwissenschaften, vol.81, pp.528-535, 1994. 11. D. J. Griffith, “Introduction to Electrodynamics,” Prentice Hall, 1999. 12. M. P. Hughes, “Nanoelectromechanics in Engineering and Biology”, CRC PRESS, 2002. 13. J. Black and G. Hastings, “Handbook of biomaterial properties”, Chapman & Hall, 1998. 14. Y. Huang, X. B. Wang, J. A. Tame and R. Pethig, “Electrokinetic behaviour of colloidal particles in travelling electric fields: studies using yeast cells”, J. Phys. D: Appl. Phys., vol.26, pp.1528-1535, 1993. 15. A. Ramos, H. Morgan, N. G. Green and A. Castellanos, “AC electrokinetics: a review of forces in microelectrode structures”, J. Phys. D: Appl. Phys., vol.31, pp.2338-2353, 1998. 16. M. Frenea, S. P. Faure, B. Le Pioufle, Ph. Coquet and H. Fujita, “Position living cells on a high-density electrode array by negative dielectrophoresis”, Material Science and Engineering, vol.23, 597-603, 2003. 17. J. Rousselet, G. H. Markx, R. Pethig, “Separation of erythrocytes and latex beads by dielectrophoretic levitation and hyperlayer field-flow fractionation”, Physicochemical and Engineering Aspects, vol.140, pp.597-603. 1997. 18. J. T. Y. Lin, J. T. W. Yeow, “Enhancing dielectrophoresis effect through novel electrode geometry”, Biomed Microdevices, vol.9, 823-831. 2007. 19. R. Pethig, Mark S. Talary and Richard S. Lee, “Enhancing Traveling-Wave Dielectrophoresis with Signal Superposition”, IEEE Engineering in Medicine and Biology Magazine, 2003. 20. H. Morgan, N. G. Green, M. P. Hughes, W. Monaghan and T. C. Tan, “Large-area travelling-wave dielectrophoresis particle separator”, J. Micromech. Microeng, vol.7, 65-70. 1997. 21. E. G. Cen, C. Dalton, Y. Li, S. Adamia, L. M. Pilarski and K. V.I.S. Kaler, “A combined dielectrophoresis, traveling wave dielectrophoresis and electrorotation microchip for the manipulation and characterization of human malignant cells”, Journal of Microbiological Methods, vol.58, pp.387-401. 2004. 22. A. D. Goater, J. P. H. Burt and R. Pethig, “A combined travelling wave dielectrophoresis and electrorotation device: applied to the concentration and viability determination of Cryptosporidium”, J. Phys. D: Appl. Phys., vol.31, pp.2338-2353, 1998. 23. D. F. Chen, H. Du, W. H. Li, “Bioparticle separation and manipulation using dielectrophoresis”, Sensors and Actuators A, vol. 133, pp.329-334, 2007. 24. 羅英傑, “介電泳應用於血漿/紅血球分離及紅血球操控之研究”, 國立台灣大學應用力學所碩士論文, 2002. 25. 陳銘昌, “旅波式介電泳幫浦之實驗研究”, 國立台灣大學應用力學所碩士論文, 2007. 26. 葉祐銘, “旅波介電泳幫浦的設計分析”, 國立台灣大學應用力學所碩士論文, 2007. 27. 黃敬文, “用以輸送血液之旅波式介電泳幫浦”, 國立台灣大學應用力學所碩士論文, 2008. 28. 朱佳仁, 施聖洋, “雷射光學量測數據時序、景深等參數分析與處理技術建立”, 行政院原子能委員會委託研究計畫論文報告, 2003. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/42335 | - |
dc.description.abstract | 本論文利用微機電技術製造旅波式介電泳幫浦及細胞分離晶片。旅波式介電泳幫浦為一截面為矩形的流道,在其中一壁面鋪上如鐵軌般垂直於流道的電極,並施以六組四相位差的交流訊號,此時紅血球將會被傳統式介電泳力抬離電極表面,旅波式介電泳力則會驅動紅血球往相位遞增的方向前進,血漿則會藉由紅血球移動時的黏滯阻力被帶動,而達到傳輸血液的效果;細胞分離晶片亦使用旅波式介電泳力,當細胞於同一位置出發,經過與流道具有特定傾斜角度的電極陣列,會產生不同幅度的偏向,即可分辨出不同的細胞。本文針對這兩部份的研究,完成的工作如下:(1)找出最佳的旅波式介電泳幫浦操作頻率,5MHz時全血平均流速可達50μm/s。(2)比較在首根電極前增加兩根輔助電極於不同情況下是否能提升幫浦的效能,結果主要顯示輔助電極可助縮短幫浦達穩態運輸時的暫態時間,如10MHz時由30分鐘縮短為20分鐘。(3)探討了血液濃度對旅波式介電泳幫浦的影響。(4)測試幾種不同的流道設計,電極區漸縮的流道有較好的表現,幫浦平均流速從原本的11.1μm/s提升至16.5μm/s。(5)本研究設計的細胞分離晶片,可在10MHz、背景流速69.44~148.81μm/s時,將RPMI培養液中的CL1-0、CL1-5和白血球分辨出來;在10MHz、背景流速49.60~128.97μm/s時,將PBS緩衝液中的Jurkat、白血球和紅血球分辨出來。 | zh_TW |
dc.description.abstract | MEMS techniques are emploged to fabricate travelling wave dielectrophoretic (TWDEP) pumps and cell separation chips. The pump is a micro channel with a rectangular cross-section with an electrode array on one of its walls. The electrodes are operated under ac signal 90º shift of between neighboring electrodes, and there are 24 basic electrodes on an array. The negative dielectrophoretic force repels the red blood cells from the electrode surface and the travelling wave dielectrophoretic force drives the cells along the direction of increasing phase. The plasma will be drag through viscous force as the red blood cells move, and the whole blood is delivered. In cell separation chips, the paths of different cells in a background flow are deflected by a designed travelling wave dielectrophoresis, and thus different cells are separated. Several works have been completed: (1) Optimum frequency for whole blood delivery in travelling wave dielectrophoretic pumps is found to be around 5MHz. (2) Two assistant electrodes before the basic electrode array can shorten the transient time of the TWDEP pump, and can enhance pump performance in some cases. (3) Performance of the TWDEP pump at different volume ratios of cells have been studied. (4) Different channel designs were carried out, and it is found that electrode array in converging channel section has better pump performance, the average velocity increases from 11.1μm/s promote to the 16.5μm/s. (5) At 10MHz and background velocity 69.44~148.81μm/s , the cell separation chip can separate CL1-0, CL1-5 and WBC in the RPMI medium ; At and background velocity 49.60~128.97μm/s, our cell separate chip can recognize Jurkat, WBC and RBC in the PBS solution. | en |
dc.description.provenance | Made available in DSpace on 2021-06-15T01:12:18Z (GMT). No. of bitstreams: 1 ntu-98-R96543036-1.pdf: 7047296 bytes, checksum: 2498d2fa8be9e192ac4023b31bb6b74a (MD5) Previous issue date: 2009 | en |
dc.description.tableofcontents | 誌謝 i
中文摘要 ii 英文摘要 iii 目錄 iv 圖表目錄 vii 第一章 緒論 1 1.1 研究動機及背景 1 1.2 研究目的 2 1.3 文獻回顧 2 1.4 本文架構 4 第二章 理論 5 2-1 有效偶極矩 5 2-2 介電泳力 6 2-3 等效圓球模型 7 2-4 正負介電泳力發生之原因 8 2-5 CM factor與全血傳輸性能的關係 9 2-6 黏滯拖曳力 10 第三章 實驗方法與設備 12 3.1 系統設計 12 3.2 運用MEMS技術製造電極晶片 12 3-2-1 清潔 12 3-2-2 電極蒸鍍 13 3-2-3 電極微影製程 13 3.3 運用MEMS技術製造微流道 14 3-3-1 流道微影製程 14 3-3-2 PDMS翻模製作 15 3.4 元件接合與外部連結方式 16 3.5 實驗設備 16 3.6 速度量測 17 3.7 生物粒子的選取 18 3-7-1 貼附型細胞的收集與次培養 19 3-7-2 純化白血球濃厚液流程 19 3.8 溶液的選取 19 3-8-1 貼附型細胞培養液的調配與保存 19 3.9 細胞正負介電泳力測試 20 第四章 結果與討論 21 4.1 旅波式介電泳幫浦 21 4-1-1 不同頻率與樣本對幫浦效能的影響 21 4-1-2 有無輔助電極穩態研究 21 4-1-3 有無輔助電極暫態研究 22 4-1-4 旅波使不同細胞粒子移動效能測試 23 4-1-5 實驗旅波式介電泳幫浦注意事項 23 4.2 全血稀釋結果 23 4-2-1 以生理食鹽水稀釋結果 24 4-2-2 以血漿稀釋結果 25 4-2-3 以不同樣本稀釋結果 26 4.3 介電泳幫浦流道改良 26 4-3-1 不同流道外型的實驗結果 26 4.4 利用旅波式介電泳分離細胞 27 4-4-1 定義偏移量 28 4-4-2 懸浮型細胞於PBS緩衝液中的偏移情形 29 4-4-3 貼附型細胞及白血球於培養液中的偏移情形 29 4-4-4 貼附型細胞黏著性對實驗結果的影響 30 第五章 結論與未來展望 31 5.1 結論 31 5.2 未來展望 33 參考文獻與書目 35 附圖表 38 附錄一 貼附型細胞的收集與繼代培養 84 附錄二 貼附型細胞培養液的調配與保存 85 附錄三 純化白血球濃厚液流程 86 | |
dc.language.iso | zh-TW | |
dc.title | 介電泳細胞分離與輸送的研究 | zh_TW |
dc.title | Study of the Cell Separation and Delivery Using Dielectrophoresis | en |
dc.type | Thesis | |
dc.date.schoolyear | 97-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 陳希立,楊政穎 | |
dc.subject.keyword | 旅波式介電泳幫浦,輔助電極,細胞分離, | zh_TW |
dc.subject.keyword | Travelling wave dielectrophoretic pump,Assistant electrode,Cell separation, | en |
dc.relation.page | 86 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2009-07-30 | |
dc.contributor.author-college | 工學院 | zh_TW |
dc.contributor.author-dept | 應用力學研究所 | zh_TW |
顯示於系所單位: | 應用力學研究所 |
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