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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 賈景山 | |
| dc.contributor.author | Yu-Shiang Hu | en |
| dc.contributor.author | 胡渝翔 | zh_TW |
| dc.date.accessioned | 2021-06-15T00:51:32Z | - |
| dc.date.available | 2016-10-05 | |
| dc.date.copyright | 2011-10-05 | |
| dc.date.issued | 2011 | |
| dc.date.submitted | 2011-08-14 | |
| dc.identifier.citation | Ahmed, R., Bevan, M.J., Reiner, S.L., and Fearon, D.T. (2009). The precursors of memory: models and controversies. Nat. Rev. Immunol. 9, 662-668.
Andreasen, J.O. (1968). Oral lichen planus. 1. A clinical evaluation of 115 cases. Oral Surg Oral. Med. Oral. Pathol. 25, 31-42. Axell, T., and Rundquist, L. (1987). Oral Lichen-Planus - a Demographic-Study. Community. Dent. Oral. 15, 52-56. Bagan-Sebastian, J.V., Milian-Masanet, M.A., Penarrocha-Diago, M., and Jimenez, Y. (1992). A clinical study of 205 patients with oral lichen planus. J. Oral. Maxillofac. Surg. 50, 116-118. Bermejo-Fenoll, A., and Lopez-Jornet, P. (2006). Familial oral lichen planus: presentation of six families. Oral. Surg. Oral. Med. Oral. Pathol. Oral. Radiol. Endod. 102, e12-15. Carrozzo (2004). Tumor necrosis factor-alpha and interferon-gamma polymorphisms contribute to susceptibility to oral lichen planus. Journal of Investigative Dermatology 123, 805-805. Carrozzo, M., Gandolfo, S., Lodi, G., Carbone, M., Garzino-Demo, P., Carbonero, C., Porter, S.R., and Scully, C. (1999). Oral lichen planus in patients infected or noninfected with hepatitis C virus: the role of autoimmunity. J. Oral. Pathol. Med. 28, 16-19. Carrozzo, M., Quadri, R., Latorre, P., Pentenero, M., Paganin, S., Bertolusso, G., Gandolfo, S., and Negro, F. (2002). Molecular evidence that the hepatitis C virus replicates in the oral mucosa. J. Hepatol. 37, 364-369. Daftary, D.K., Bhonsle, R.B., Murti, R.B., Pindborg, J.J., and Mehta, F.S. (1980). An oral lichen planus-like lesion in Indian betel-tobacco chewers. Scand. J. Dent. Res. 88, 244-249. Dejong, R., Brouwer, M., Miedema, F., and Vanlier, R.A.W. (1991). Human Cd8+ Lymphocytes-T Can Be Divided into Cd45ra+ and Cd45ro+ Cells with Different Requirements for Activation and Differentiation. J. Immunol. 146, 2088-2094. Eisen, D. (1999). The evaluation of cutaneous, genital, scalp, nail, esophageal, and ocular involvement in patients with oral lichen planus. Oral. Surg. Oral. Med. Oral. Pathol. Oral. Radiol. Endod. 88, 431-436. Eisen, D., Carrozzo, M., Bagan Sebastian, J.V., and Thongprasom, K. (2005). Number V Oral lichen planus: clinical features and management. Oral. Dis. 11, 338-349. Hamann, D., Baars, P.A., Rep, M.H., Hooibrink, B., Kerkhof-Garde, S.R., Klein, M.R., and van Lier, R.A. (1997). Phenotypic and functional separation of memory and effector human CD8+ T cells. J. Exp. Med. 186, 1407-1418. Harigai, M., Kawamoto, M., Hara, M., Kubota, T., Kamatani, N., and Miyasaka, N. (2008). Excessive production of IFN-gamma in patients with systemic lupus erythematosus and its contribution to induction of B lymphocyte stimulator/B cell-activating factor/TNF ligand superfamily-13B. J. Immunol. 181, 2211-2219. Holmstrup, P. (1992). The Controversy of a Premalignant Potential of Oral Lichen-Planus Is Over. Oral. Surg. Oral. Med. 73, 704-706. Ikeda, N., Ishii, T., Iida, S., and Kawai, T. (1991). Epidemiological study of oral leukoplakia based on mass screening for oral mucosal diseases in a selected Japanese population. Community. Dent. Oral. Epidemiol. 19, 160-163. Ismail, S.B., Kumar, S.K., and Zain, R.B. (2007). Oral lichen planus and lichenoid reactions: etiopathogenesis, diagnosis, management and malignant transformation. J. Oral. Sci. 49, 89-106. Jungell, P., Konttinen, Y.T., Nortamo, P., and Malmstrom, M. (1989). Immunoelectron microscopic study of distribution of T cell subsets in oral lichen planus. Scand. J. Dent. Res. 97, 361-367. Kagi, D., Vignaux, F., Ledermann, B., Burki, K., Depraetere, V., Nagata, S., Hengartner, H., and Golstein, P. (1994). Fas and Perforin Pathways as Major Mechanisms of T-Cell-Mediated Cytotoxicity. Science 265, 528-530. Karagouni, E.E., Dotsika, E.N., and Sklavounou, A. (1994). Alteration in peripheral blood mononuclear cell function and serum cytokines in oral lichen planus. J. Oral. Pathol. Med. 23, 28-35. Khan, A., Farah, C.S., Savage, N.W., Walsh, L.J., Harbrow, D.J., and Sugerman, P.B. (2003). Th1 cytokines in oral lichen planus. J. Oral. Pathol. Med. 32, 77-83. Kieper, W.C., Tan, J.T., Bondi-Boyd, B., Gapin, L., Sprent, J., Ceredig, R., and Surh, C.D. (2002). Overexpression of interleukin (IL)-7 leads to IL-15-independent generation of memory phenotype CD8+ T cells. J. Exp. Med. 195, 1533-1539. Kilpi, A. (1988). Characterization of mononuclear cells of inflammatory infiltrates in oral tissues. A histochemical and immunohistochemical study of labial salivary glands in Sjogren's syndrome and of oral lesions in systemic lupus erythematosus and in lichen planus. Proc. Finn. Dent. Soc. 84 Suppl 3, 1-93. Kilpi, A.M. (1987). Activation marker analysis of mononuclear cell infiltrates of oral lichen planus in situ. Scand. J. Dent. Res. 95, 174-180. Kirby, A.C., Lodi, G.L., Olsen, I., and Porter, S.R. (1998). Immunohistochemical and serological comparison of idiopathic and hepatitis C virus-associated forms of oral lichen planus. Eur. J. Oral. Sci. 106, 853-862. Kurokawa, M., Hidaka, T., Sasaki, H., Nishikata, I., Morishita, K., and Setoyama, M. (2003). Analysis of hepatitis C virus (HCV) RNA in the lesions of lichen planus in patients with chronic hepatitis C: detection of anti-genomic- as well as genomic-strand HCV RNAs in lichen planus lesions. J. Dermatol. Sci. 32, 65-70. Lin, H.C., Corbet, E.F., and Lo, E.C. (2001). Oral mucosal lesions in adult Chinese. J. Dent. Res. 80, 1486-1490. Lin, S.C., and Sun, A. (1990). HLA-DR and DQ antigens in Chinese patients with oral lichen planus. J. Oral. Pathol. Med. 19, 298-300. Lind, P.O., Stromme Koppang, H., and Aas, E. (1985). Malignant transformation in oral lichen planus. Int. J. Oral. Surg. 14, 509-516. Lowin, B., Hahne, M., Mattmann, C., and Tschopp, J. (1994). Cytolytic T-Cell Cytotoxicity Is Mediated through Perforin and Fas Lytic Pathways. Nature 370, 650-652. Mackall, C.L., Fry, T.J., and Gress, R.E. (2011). Harnessing the biology of IL-7 for therapeutic application. Nat. Rev. Immunol. 11, 330-342. Matthews, J.B., Scully, C.M., and Potts, A.J.C. (1984). Oral Lichen Planus - an Immunoperoxidase Study Using Monoclonal-Antibodies to Lymphocyte Subsets. Brit J. Dermatol. 111, 587-595. Mazzucchelli, R., and Durum, S.K. (2007). Interleukin-7 receptor expression: intelligent design. Nat. Rev. Immunol. 7, 144-154. McCartan, B.E. (1995). Psychological factors associated with oral lichen planus. J. Oral. Pathol. Med. 24, 273-275. McKinney, E.F., Lyons, P.A., Carr, E.J., Hollis, J.L., Jayne, D.R., Willcocks, L.C., Koukoulaki, M., Brazma, A., Jovanovic, V., Kemeny, D.M., et al. (2010a). A CD8+ T cell transcription signature predicts prognosis in autoimmune disease. Nat. Med. 16, 586-591, 581p following 591. McKinney, E.F., Lyons, P.A., Carr, E.J., Hollis, J.L., Jayne, D.R.W., Willcocks, L.C., Koukoulaki, M., Brazma, A., Jovanovic, V., Kemeny, D.M., et al. (2010b). A CD8+ T cell transcription signature predicts prognosis in autoimmune disease. Nat. Med. 16, 586-591. Murti, P.R., Daftary, D.K., Bhonsle, R.B., Gupta, P.C., Mehta, F.S., and Pindborg, J.J. (1986). Malignant potential of oral lichen planus: observations in 722 patients from India. J. Oral. Pathol. 15, 71-77. Pilli, M., Penna, A., Zerbini, A., Vescovi, P., Manfredi, M., Negro, F., Carrozzo, M., Mori, C., Giuberti, T., Ferrari, C., and Missale, G. (2002). Oral lichen planus pathogenesis: A role for the HCV-specific cellular immune response. Hepatology 36, 1446-1452. Schlosser, B.J. (2010). Lichen planus and lichenoid reactions of the oral mucosa. Dermatol. Ther. 23, 251-267. Scully, C., Beyli, M., Ferreiro, M.C., Ficarra, G., Gill, Y., Griffiths, M., Holmstrup, P., Mutlu, S., Porter, S., and Wray, D. (1998). Update on oral lichen planus: etiopathogenesis and management. Crit. Rev. Oral. Biol. Med. 9, 86-122. Silverman, S., Jr., Gorsky, M., and Lozada-Nur, F. (1985). A prospective follow-up study of 570 patients with oral lichen planus: persistence, remission, and malignant association. Oral. Surg. Oral. Med. Oral. Pathol. 60, 30-34. Silverman, S., Jr., Gorsky, M., Lozada-Nur, F., and Giannotti, K. (1991). A prospective study of findings and management in 214 patients with oral lichen planus. Oral. Surg. Oral. Med. Oral. Pathol. 72, 665-670. Sugerman, P.B., Satterwhite, K., and Bigby, M. (2000). Autocytotoxic T-cell clones in lichen planus. Br. J. Dermatol. 142, 449-456. Sugerman, P.B., Savage, N.W., Walsh, L.J., Zhao, Z.Z., Zhou, X.J., Khan, A., Seymour, G.J., and Bigby, M. (2002). The pathogenesis of oral lichen planus. Crit. Rev. Oral. Biol. Med. 13, 350-365. Tan, J.T., Ernst, B., Kieper, W.C., LeRoy, E., Sprent, J., and Surh, C.D. (2002). Interleukin (IL)-15 and IL-7 jointly regulate homeostatic proliferation of memory phenotype CD8(+) cells but are not required for memory phenotype CD4(+) cells. J. Exp. Med. 195, 1523-1532. Thorn, J.J., Holmstrup, P., Rindum, J., and Pindborg, J.J. (1988). Course of Various Clinical Forms of Oral Lichen Planus - a Prospective Follow-up-Study of 611 Patients. J. Oral. Pathol. Med. 17, 213-218. Thornhill, M.H., Sankar, V., Xu, X.J., Barrett, A.W., High, A.S., Odell, E.W., Speight, P.M., and Farthing, P.M. (2006). The role of histopathological characteristics in distinguishing amalgam-associated oral lichenoid reactions and oral lichen planus. J. Oral. Pathol. Med. 35, 233-240. van der Meij, E.H., Schepman, K.P., Smeele, L.E., van der Wal, J.E., Bezemer, P.D., and van der Waal, I. (1999). A review of the recent literature regarding malignant transformation of oral lichen planus. Oral. Surg. Oral. Med. Oral. Pathol. Oral. Radiol. Endod. 88, 307-310. Veien, N.K., Risum, G., Jorgensen, H.P., and Svejgaard, A. (1979). HLA antigens in patients with lichen planus. Acta. Derm. Venereol. 59, 205-209. Vincent, S.D., Fotos, P.G., Baker, K.A., and Williams, T.P. (1990). Oral lichen planus: the clinical, historical, and therapeutic features of 100 cases. Oral. Surg. Oral. Med. Oral. Pathol. 70, 165-171. Vukmanovic-Stejic, M., Vyas, B., Gorak-Stolinska, P., Noble, A., and Kemeny, D.M. (2000). Human Tc1 and Tc2/Tc0 CD8 T-cell clones display distinct cell surface and functional phenotypes. Blood 95, 231-240. White, A.G., and Rostom, A.I. (1994). HLA antigens in Arabs with lichen planus. Clin. Exp. Dermatol. 19, 236-237. Williams, N.S., and Engelhard, V.H. (1997). Perforin-dependent cytotoxic activity and lymphokine secretion by CD4+ T cells are regulated by CD8+ T cells. J. Immunol. 159, 2091-2099. Woo, V., Bonks, J., Borukhova, L., and Zegarelli, D. (2009). Oral lichenoid drug eruption: a report of a pediatric case and review of the literature. Pediatr. Dermatol. 26, 458-464. Zakrzewska, J.M., Chan, E.S.Y., and Thornhill, M.H. (2005). A systematic review of placebo-controlled randomized clinical trials of treatments used in oral lichen planus. Brit. J. Dermatol. 153, 336-341. Zhang, J. (2007). Yin and yang interplay of IFN-gamma in inflammation and autoimmune disease. J. Clin. Invest. 117, 871-873. Zhou, X.J., Sugerman, P.B., Savage, N.W., Walsh, L.J., and Seymour, G.J. (2002). Intra-epithelial CD8+ T cells and basement membrane disruption in oral lichen planus. J. Oral. Pathol. Med. 31, 23-27. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/42186 | - |
| dc.description.abstract | 口腔扁平苔癬是一種病因仍不明瞭之口腔黏膜自體免疫疾病。國外研究發現從病人病灶部位分離出之CD8+ T 細胞對自體口腔黏膜的角質層細胞具有毒殺能力。最近的研究指出紅斑性狼瘡病人體中的CD8+ T細胞有較高的IL-7受體表現。因此本研究探討在口腔扁平苔癬病人中的周邊血液CD45RA- CD8+ T細胞與CD45RA+ CD8+ T細胞IL-7受體表現以及製造細胞激素的能力。分選出之周邊血液IL-7R+ CD45RA- CD8+ T 細胞利用流式細胞儀染色分析或是分離出之細胞以anti-CD3加上自體抗原呈現細胞共同培養並以ELISA測定之。病人的CD8+ T 細胞相對於控制組有較高的IFN-γ以及granzyme B表現量但FasL在病人與控制組並無差異。病人的CD8+ T 細胞含有高量IFN-γ以及granzyme B 被認為具有毒殺目標細胞的能力。事實上，在細胞毒殺能力的實驗中，病人的CD8+ T 細胞有較高的毒殺能力。此外病人CD8+ T 細胞有較高的IL-7受體表現與較高的IFN-γ 和 granzyme B 在病人的CD8+ T 細胞具高度正相關，可能是因為IL-7受體能幫助記憶性T細胞數量的恆定。另一方面，病人有較高的CD4+ IL-17細胞表現而且同樣的與高量的IFN-γ CD8+ T 細胞是有高度正相關，而且CD4+ T細胞分泌較少的IL-10。從以上的發現顯示周邊血液記憶性CD8+ T 細胞參與口腔扁平苔癬中的致病機轉而且可能是一項造成疾病復發的風險因素。 | zh_TW |
| dc.description.abstract | Oral lichen planus (OLP) is an autoimmune oral mucosal disease of unknown etiology. In vitro study shows that CD8+ T Cells from Oral lichen planus (OLP) patient’s lesion sites are cytotoxic to autologous keratinocytes. A recent report implicated that CD8+ T cells from patients with recurrent SLE exhibited up-regulated IL-7R. We examined the expression IL-7R of peripheral CD45RA- CD8+ T cells and CD45RA+ CD8+ T cells in patient with OLP and analyzed cytokine production. Peripheral blood IL-7R+ CD45RA- CD8+ T cells were sorted and the cytokine profiles were detected by flow cytometry, or ELISA after stimulation with anti-CD3 mAb in the presence of autologous APCs. Killing activity will be tested using mouse Mastocytoma P815 cell line. CD8+ T cells from OLP patients had increased IFN-γ production in contrast to healthy donor CD8+ T cells whereas expression of FasL was similar. The CD8+ T cells in OLP patient had increased IFN-γ production and granzyme B were able to trigger target cells apoptosis, in addition, using P815 cytotoxicity assay patient CD8+ T cells had higher killing ability, suggested that CD8+ T cells had enhanced effector function. CD8+ T cells in patient had higher IL-7R expression that correlated with level of IFN-γ production and granzyme B expression, to maintain memory phenotype of CD8+ T cells. On the other hand, higher frequency of IL-17 producing CD4+ T cells was also observed and correlated with increased CD8 IFN-γ production. IL-10 production from CD4+ T cells cultured supernatant was lower than healthy donors. Taken together, these data suggested that the overexpression of IFN-γ in peripheral blood memory CD8+ T cells may contribute to the immunopathogenesis of OLP and would be a risk factor of this relapsing disease. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-15T00:51:32Z (GMT). No. of bitstreams: 1 ntu-100-R98449013-1.pdf: 1541933 bytes, checksum: 1341c7c95b7a21417d0c5f3e1ef6794e (MD5) Previous issue date: 2011 | en |
| dc.description.tableofcontents | 口試委員會審定書 I
致謝 II 中文摘要 III Abstract IV Chapter 1. Introduction 3 1.1 Oral Lichen planus (OLP) 4 1.2 Epidemiology Oral Lichen planus 6 1.3 Etiology of OLP 7 1.4 Malignant transformation of OLP 9 1.5 Immunopathogenesis of Oral lichen planus 10 1.6 CD8+ T cells in Lesion Site 10 1.7 Memory cells in OLP 11 Chapter 2. Material and methods 13 2.1 Human subjects 13 2.2 Peripheral Blood Mononuclear Cell isolation 13 2.3 Flow cytometry analysis (FACS) 13 2.4 Cell sorting 14 2.5 Stimulation of T cells 15 2.6 Cytotoxicity Assay 15 2.7 ELISA 16 2.8 Statistical analysis 16 Chapter 3. Results 17 Chapter 4. Discussion 26 Chapter 5. References 30 Chapter 6. Figures 36 圖目錄 Figure 1. CD8+ T cells in OLP patients had increased IFN-γ production and Granzyme B expression. 36 Figure 2. CD8+ T cells in OLP patients had higher cytotoxic capacity. 37 Figure 3. Fas ligand expression in CD8+ T cells. 38 Figure 4. CD8+ T cells from OLP patient had higher IL-7R expression in memory population…………………………………………………………………………………………………………………………… …39Figure 5. CD8+ T cells in OLP patient had higher memory cells and IL-7R expressions were correlated with level of IFN-γ production and granzyme B expression………………………………………………………………………………………………………………….…….……. 40 Figure 6. CD8+ CD45RA–IL-7R+ T cells had elevated IFN-γ production……….. 41 Figure 7. Both CD8+ CD45RA–IL-7R+ and CD8+ CD45RA+IL-7R+ T cells had elevated IFN-r or Granzyme B expression. 42 Figure 8. CD8+ T cells from OLP patient had higher IL-7R expression in memory population……………………………………………………………………………………………………………………………… 43 Figure 9. IL-17 and IL-10-producing CD4+ T cells in OLP patients. 44 Figure 10. IL-17+ CD4+ T cells correlate to IFN-r+CD8+ T cells. 45 | |
| dc.language.iso | en | |
| dc.title | 口腔扁平苔癬病人周邊血液毒殺性淋巴球之特性分析 | zh_TW |
| dc.title | Functional characterization of peripheral blood CD8+ T cells from patients with oral lichen planus | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 99-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 許秉寧,王莉芳 | |
| dc.subject.keyword | 口腔扁平苔癬,毒殺性淋巴球, | zh_TW |
| dc.subject.keyword | oral lichen planus,CD8+ T cells, | en |
| dc.relation.page | 45 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2011-08-15 | |
| dc.contributor.author-college | 醫學院 | zh_TW |
| dc.contributor.author-dept | 免疫學研究所 | zh_TW |
| 顯示於系所單位： | 免疫學研究所 | |
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