請用此 Handle URI 來引用此文件:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/41972
標題: | TDP-43大量表現對於包含SMN基因之Pre-mRNA的Exon-7剪接之增益 TDP-43 Overexpression Enhances Exon-7 Inclusion during SMN Pre-mRNA Splicing |
作者: | Bose Jayarama Krishnan 賈亞 |
指導教授: | 沈哲鯤 |
關鍵字: | SMN基因, SMN Exon-7, |
出版年 : | 2008 |
學位: | 博士 |
摘要: | TDP-43 is a highly conserved, 43 kDa RNA-binding protein implicated to play a role in transcription repression, nuclear organization, and alternative splicing. More recently, TDP-43 has been identified as the major disease protein of several neurodegenerative diseases including frontotemporal lobar degeneration with ubiquitin-positive inclusions (FTLD-U) and amyotrophic lateral sclerosis (ALS). For the splicing activity, TDP-43 has been shown to be mainly an exon-skipping promoter. In this study using the Survival of Motor Neuron (SMN) minigenes as the reporters in transfection assay, I show for the first time that TDP-43 could also act as an exon-inclusion factor. Furthermore, both RRM domains are required for the ability of TDP-43 to enhance the SMN2 exon 7-inclusion. Combined protein-immunoprecipitation (IP) and RNA-IP experiments also suggested that the exon-inclusion by TDP-43 might be mediated by multimeric complex(es) consisting of TDP-43 interacting with other splicing factors including Htra2-β1. My data further evidences TDP-43 as a multifunctional
RNA-binding protein for a diverse set of cellular activities. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/41972 |
全文授權: | 有償授權 |
顯示於系所單位: | 分子醫學研究所 |
文件中的檔案:
檔案 | 大小 | 格式 | |
---|---|---|---|
ntu-97-1.pdf 目前未授權公開取用 | 2.6 MB | Adobe PDF |
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。