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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 紀秀華(Shiou-Hwa Jee) | |
dc.contributor.author | Pei-Lun Sun | en |
dc.contributor.author | 孫培倫 | zh_TW |
dc.date.accessioned | 2021-06-15T00:30:08Z | - |
dc.date.available | 2011-02-10 | |
dc.date.copyright | 2009-02-10 | |
dc.date.issued | 2009 | |
dc.date.submitted | 2009-01-19 | |
dc.identifier.citation | 1. Ainsworth G.C. Introduction to the history of medical and veterinary mycology. Cambridge university press. 1986
2. Ajello L, Cheng SL, The perfect state of Trichophyton mentagrophytes. Sabouraudia 1967;5:230-234 3. Ajello L, Bostic L, Cheng SL, The relationship of Trichophyton quinckeanum to Trichophyton mentagrophytes. Mycologia, 1968;60(6):1185-1189 4. Balajee SAM, Ranganathans S, Menont T. Soil dermatophytes in Madras, India, in relation to human ringworm. Mycoses 1997;40(7-8):317-320 5. Bocobo FC, Curtis AC, Accidental isolation of Trichophyton mentagrophytes from the floor of a schoolhouse. Mycologia, 1958;50:164-168 6. Brilhante RSN, Cordeiro RA, Gomes JMF, Sidrim JJC, Rocha MFG, Canine dermatophytosis caused by an anthropophilic species: molecular and phenotypical characterization of Trichophyton tonsurans. Journal of Medical Microbiology 2006; 55:1583–1586 7. CBS website: www.cbs.knaw.nl 8. de Hoog GS, Guarro J, Gené J et al, Atlas of clinical fungi, 2nd ed. Centraalbureau voor Schimmelcultures, Utrecht, The Netherlands & Universitat Rovira I Virgili, Reus, Spain, 2000 9. DeLamater ED, The squirrel as a new host to a ringworm fungus. Mycology 1939;31:519-526 10. Gräser Y, Kuijpers AFA, Presber W, et al, Molecular taxonomy of Trichophyton mentagrophytes and T. tonsurans. Medical mycology 1999;37:315-330 11. Kac G, Molecular approaches to the study of dermatophytes. Medical mycology 2000;38:329-336 12. Kane J, Summerbell R, Sigler L, Krajden S, Land G, Laboratory hand book of dermatophytes. Star publishing company. 1997 13. Kushida T, Watanabe S, Canine ringworm caused by Trichophyton rubrum; probable transmission from man to animal. Sabouraudia 1975;13:30-32 14. Li HC, Bouchara J-P, Hsu ML, Barton R, Su S, Chang TC. Identification of dermatophytes by sequence analysis of the rRNA internal transcribed spacer regions. Journal of medical microbiology 2008;57:592-600 15. Lurie HI, Borok R, Trichophyton mentragphytes isolated from the soil of caves. Mycologia 1955,47:506-510 16. Marple MJ, Smith JMB, The hedgehog as a source of human ringworm. Nature 1960;188:867-868 17. Padhye AA, Sekhon AS, Carmichael JW, Ascocarp production by Nannizzia and Arthroderma on keratinous and non-keratinous media. Sabouraudia 1973;11:109-114 18. Padhye AA, Ajello L, The taxonomic status of the hedgehog fungus Trichophyton erinacei. Sabouraudia 1977;15:103-114 19. Priestley H. Ringworm and allied parasitic skin diseases in Australia. The medical journal of Australia. 1917;2:471-475 20. Quaife RA, Human infection due to the hedgehog fungus, Trichophyton mentagrophytes var. erinacei. Journal of clinical pathology 1966;19:177-178 21. Smith JMB, Marples MJ, Trichophyton mentagrophytes var. erinacei. Sabouraudia 1963;3:1-10 22. Swofford, D. L.. PAUP*. Phylogenetic Analysis Using Parsimony (*and Other Methods). Version 4. Sinauer Associates, Sunderland, Massachusetts. 2002 23. Summerbell RC, Weitzman I, Padhye AA, The Trichophyton mentagrophytes complex: biological species and mating type prevalences of North American isolates, and a review of the worldwide distribution and host associations of species and mating types. Study in Mycology 2002;47:75-86 24. Takashio M, Une nouvelle forme sexuée due complex Trichophyton mentagrophytes, Arthroderma vanbreuseghemii sp. nov.. Ann. Parasitol. Humaine Comp. 1973;48:713-732 25. Takashio M, Observations on Afreican and European strains of Arthroderma benhamiae. Dermatology 1974;13:94-101 26. Terreni AA, Gregg WB Jr., Morris PR, Disalvo AF, Epidermophyton floccosum infection in a dog from the United States. Sabouraudia 1985;23(2):141-142 27. Thompson,J.D., Gibson,T.J., Plewniak,F., Jeanmougin,F. and Higgins,D.G. The ClustalX windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Research. 1997; 24:4876-4882 28. Torres-Rodríguez JM, Dronda MA, Rossell J, Madrenys N. Incidence of dermatophytoses in rabbit farms in Catalonia, Spain, and its repercussion on human health. European journal of epidemiology. 1992;8(3):326-329 | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/41756 | - |
dc.description.abstract | 鬚癬毛癬菌複合種屬於皮癬菌,為重要的致病真菌,在臨床上可造成各樣的皮膚感染,如頭癬、臉癬、體癬、手癬、足癬、甲癬等。其宿主範圍極廣,可寄生於人類、貓、狗、鼠、刺蝟身上,利用人類及動物身上的角質物質為營養來源。傳染途徑為人與人、及人與動物直接或間接的接觸造成感染,為公共衛生上一個重要的問題。因此,正確的真菌培養及檢定,有助於了解感染的來源,並加以阻斷,使病人得到完全的治療。
鬚癬毛癬菌複合種傳統上包括三個主要的種:鬚癬毛癬菌、趾間毛癬菌、及刺蝟毛癬菌。由於三者在形態學上相似,且存在著許多的中間型及變異型,而使此一複合種成員的命名產生相當大的混淆,同時使三者的分類地位無法完全釐清。這不僅造成在研究時我們可能會選到錯誤的菌株,也使得菌種統計數據變得不可靠,阻礙了對此菌的研究。 本實驗利用台灣本土人類及動物分離出的48株鬚癬毛癬菌複合種菌株,進行研究。除了分析臨床表現與菌種形態的關係外,我們並根據Gräser等人最新的分類方法,使用ITS1-5.8S-ITS2區段及β-tubulin基因及進行分類分析。結果發現,依最新的分類方法,48株鬚癬毛癬菌複合種中,21株屬於趾間毛癬菌人類株分群,22株屬於趾間毛癬菌動物株分群,5株為刺蝟毛癬菌,沒有鬚癬毛癬菌。由於這種分類方法與我們傳統使用的習慣不同,會造成檢定上的混淆,因此我們認為此分類方法的適用性仍待進一步的評估。 | zh_TW |
dc.description.abstract | Trichophyton mentagrophytes complex, belonging to dermatophytes, is a group of important pathogenic fungi that can cause various human skin infections such as tinea capitis, tinea faciei, tinea corporis, tinea manuum, tinea pedis and onychomycosis. They have a wide range of hosts, including human, cats, dogs, rats, and hedgehogs and can use the keratinous substracts from the hosts as the nutrition source. This is an important public health issue because they can transmit between humans and between human and animals by direct or indirect contact. Culturing and accurate identification of these fungi can help to clarify the routes of infection. This is also very important in the treatment of patients.
Traditionally, T. mentagrophytes complex consists of three main species, viz. T. mentagrophytes, T. interdigitale and T. erinacei. Due to morphological similarity and existing intermediate forms and variants, there is a great confusion in their taxonomic status. This confusion leads to trouble in choosing correct strains for research and the epidemiological statistics of fungal infections by this group of fungi and further impeding our communicating about it. In this study, we used 48 isolates of the T. mentagrophytes complex obtained from human and animals in Taiwan to perform clinical analysis and molecular taxonomy. We examined the correlation between morphology of the isolates and the clinical presentations. We also used ITS1-5.8S-ITS2 andβ-tubulin gene regions for molecular taxonomy according to the latest taxonomic definition of the T. mentagrophytes complex proposed by Gräser et. al. By this definition, our result shows that among 48 isolates, 21 isolates belong to the anthropophilic strain of T. interdigitale; 22 isolates belong to the zoophilic strain of T. interdigitale; and 5 isolates belong to T. erinacei. No T. mentagrophytes was found. Because the new classification is quite different from what it used to be, confusion in the nomenclature of the T. mentagrophytes complex will be expected. The applicability of Gräser’s taxonomic concept requires further evaluation. | en |
dc.description.provenance | Made available in DSpace on 2021-06-15T00:30:08Z (GMT). No. of bitstreams: 1 ntu-98-P95421013-1.pdf: 2799329 bytes, checksum: ef77b6db6e381f007410149842877c2e (MD5) Previous issue date: 2009 | en |
dc.description.tableofcontents | 口試委員會審定書 i
國家圖書館碩博士論文授權書 ii 誌謝 iii 目錄 iv 中文摘要 vi Abstract vii 第一章 緒論 1 1.1. 真菌感染症 1 1.2. 真菌培養及檢定在診斷及治療皮膚真菌感染症的重要性 1 1.3. Trichophyton mentagrophytes comlex簡介 2 1.3.1. Trichophyton mentagrophytes (C.P. Robin) R. Blanchard. 2 1.3.2. Trichophyton interdigitale Priestley 3 1.3.3. Trichophyton erinacei (J.M.B. Sm. & Marple) Quaife 3 1.4. Trichophyton mentagrophytes complex分類 4 1.4.1. 形態學分類 4 1.4.2. 生物種的分類(Taxonomy by biological species) 5 1.4.3. 分子分類 6 第二章 研究方法與材料 9 2.1. 菌株分離 9 2.1.1. 人類分離株 9 2.1.2. 動物分離株 9 2.2. 形態學檢定 10 2.2.1. Trichophyton mentagrophytes 10 2.2.2. Trichophyton interdigitale 10 2.2.3. Trichophyton erinacei 10 2.3. 分子研究 11 2.3.1. 培養菌絲 11 2.3.2. 萃取DNA 11 2.3.3. 聚合酶鏈結反應(polymerase chain reaction, PCR) 11 2.3.4. 純化 12 2.3.5. 定序 13 2.3.6. 分子分類分析 13 第三章 結果 14 3.1. 菌株分離 14 3.1.1. 人體分離株(表一) 14 3.1.2. 動物分離株(表二) 15 3.2. 形態學檢定 16 3.2.1. 菌落形態 16 3.2.2. 菌株顯微構造 17 3.3. 分子研究 17 3.3.1. ITS1-5.8S-ITS2區段(圖2.3.1) 18 3.3.2. β-tubulin基因(圖2.3.2) 19 第四章 討論 20 4.1. 臨床表現 20 4.2. 菌種形態分析 21 4.3. 分子分類結果 21 4.4. 特殊菌種討論 22 4.4.1. MCCF 74.08天竺鼠分離株 22 4.4.2. MCCF 39.05 人類手癬分離株 22 4.4.3. 手癬分離株 23 4.4.4. Trichophyton mentagrophytes新模式菌種CBS 318.56 23 4.5. 對現行檢定法的衝擊 25 展望 27 圖表 28 表一、人類來源菌株資料表 28 表二、動物來源菌株資料表 29 表三、人類Trichophyton interdigitale人類株分群患者資料 30 表四、人類Trichophyton interdigitale動物株分群患者資料 31 圖2.3.1 Consensus tree of maximal parsimony according to ITS1-5.8S-ITS2 regions of rDNA 32 圖2.3.2 Consensus tree of maximal parsimony according to β-tubulin gene 33 圖4.3.1. Alignment of ITS1-5.8S-ITS2 regions of anthropophilic and zoophilic strains of T. interdigitale 34 圖4.3.2. Alignment of β-tubulin gene of anthropophilic and zoophilic strains of T. interdigitale 35 圖4.4.4. Consensus tree of maximal parsimony according to ITS1-5.8S-ITS2 regions of rDNA 36 參考文獻 37 附錄 40 | |
dc.language.iso | zh-TW | |
dc.title | 鬚癬毛癬菌複合種之研究 | zh_TW |
dc.title | Trichophyton mentagrophytes complex | en |
dc.type | Thesis | |
dc.date.schoolyear | 97-1 | |
dc.description.degree | 碩士 | |
dc.contributor.coadvisor | 朱宇敏(Yu-Ming Ju) | |
dc.contributor.oralexamcommittee | 邱顯清(Chiu Hsien-Ching) | |
dc.subject.keyword | 鬚癬毛癬菌,趾間毛癬菌,刺蝟毛癬菌,分類, | zh_TW |
dc.subject.keyword | T. mentagrophytes,T. interdigitale,T. erinacei,taxnonomy, | en |
dc.relation.page | 88 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2009-01-19 | |
dc.contributor.author-college | 醫學院 | zh_TW |
dc.contributor.author-dept | 臨床醫學研究所 | zh_TW |
顯示於系所單位: | 臨床醫學研究所 |
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