請用此 Handle URI 來引用此文件:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/41685
完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 朱瑞民 | |
dc.contributor.author | Mo-Fan Chen | en |
dc.contributor.author | 陳墨繁 | zh_TW |
dc.date.accessioned | 2021-06-15T00:27:35Z | - |
dc.date.available | 2009-02-03 | |
dc.date.copyright | 2009-02-03 | |
dc.date.issued | 2009 | |
dc.date.submitted | 2009-01-21 | |
dc.identifier.citation | References
1. Wu, L. and Y.J. Liu, Development of dendritic-cell lineages. Immunity, 2007. 26(6): p. 741-50. 2. Yi, H., et al., Dendritic cells induced in the presence of GM-CSF and IL-5. Cytokine, 2007. 37(1): p. 35-43. 3. Wijewardana, V., et al., Generation of canine dendritic cells from peripheral blood monocytes without using purified cytokines. Vet Immunol Immunopathol, 2006. 114(1-2): p. 37-48. 4. Fujimoto, Y. and T.F. Tedder, CD83: a regulatory molecule of the immune sys-tem with great potential for therapeutic application. J Med Dent Sci, 2006. 53(2): p. 85-91. 5. Lechmann, M., et al., CD83 on dendritic cells: more than just a marker for maturation. Trends Immunol, 2002. 23(6): p. 273-5. 6. Harnett, M.M., CD40: a growing cytoplasmic tale. Sci STKE, 2004. 2004(237): p. pe25. 7. Ito, M., et al., Tumor-derived TGFbeta-1 induces dendritic cell apoptosis in the sentinel lymph node. J Immunol, 2006. 176(9): p. 5637-43. 8. Fogel-Petrovic, M., et al., Physiological concentrations of transforming growth factor beta1 selectively inhibit human dendritic cell function. Int Im-munopharmacol, 2007. 7(14): p. 1924-33. 9. Murgia, C., et al., Clonal origin and evolution of a transmissible cancer. Cell, 2006. 126(3): p. 477-87. 10. Chu, R.M., et al., Proliferation characteristics of canine transmissible venereal tumor. Anticancer Res, 2001. 21(6A): p. 4017-24. 11. Hsiao, Y.W., et al., Tumor-infiltrating lymphocyte secretion of IL-6 antagonizes tumor-derived TGF-beta 1 and restores the lymphokine-activated killing activity. J Immunol, 2004. 172(3): p. 1508-14. 12. Liu, C.C., et al., Transient downregulation of monocyte-derived dendritic-cell differentiation, function, and survival during tumoral progression and regression in an in vivo canine model of transmissible venereal tumor. Cancer Immunol Immunother, 2008. 57(4): p. 479-91. 13. Lin, C.Y., et al., Combined immunogene therapy of IL-6 and IL-15 enhances anti-tumor activity through augmented NK cytotoxicity. Cancer Lett, 2008. 14. Hsiao, Y.W., et al., Interactions of host IL-6 and IFN-gamma and can-cer-derived TGF-beta1 on MHC molecule expression during tumor spontane-ous regression. Cancer Immunol Immunother, 2008. 57(7): p. 1091-104. 15. Li, M.O., et al., Transforming growth factor-beta regulation of immune res-ponses. Annu Rev Immunol, 2006. 24: p. 99-146. 16. Yang, L., et al., Abrogation of TGF beta signaling in mammary carcinomas recruits Gr-1+CD11b+ myeloid cells that promote metastasis. Cancer Cell, 2008. 13(1): p. 23-35. 17. Wrzesinski, S.H., Y.Y. Wan, and R.A. Flavell, Transforming growth factor-beta and the immune response: implications for anticancer therapy. Clin Cancer Res, 2007. 13(18 Pt 1): p. 5262-70. 18. Clarke, D.C., M.D. Betterton, and X. Liu, Systems theory of Smad signalling. Syst Biol (Stevenage), 2006. 153(6): p. 412-24. 19. Widmann, C., et al., Mitogen-activated protein kinase: conservation of a three-kinase module from yeast to human. Physiol Rev, 1999. 79(1): p. 143-80. 20. Chang, L. and M. Karin, Mammalian MAP kinase signalling cascades. Nature, 2001. 410(6824): p. 37-40. 21. Ibarrola, N., et al., Cloning of a novel signaling molecule, AMSH-2, that po-tentiates transforming growth factor beta signaling. BMC Cell Biol, 2004. 5: p. 2. 22. Arteaga, C.L., et al., Transforming growth factor beta: potential autocrine growth inhibitor of estrogen receptor-negative human breast cancer cells. Cancer Res, 1988. 48(14): p. 3898-904. 23. Glasgow, E. and L. Mishra, Transforming growth factor-beta signaling and ubiquitinators in cancer. Endocr Relat Cancer, 2008. 15(1): p. 59-72. 24. Ebisawa, T., et al., Smurf1 interacts with transforming growth factor-beta type I receptor through Smad7 and induces receptor degradation. J Biol Chem, 2001. 276(16): p. 12477-80. 25. Bonni, S., et al., TGF-beta induces assembly of a Smad2-Smurf2 ubiquitin li-gase complex that targets SnoN for degradation. Nat Cell Biol, 2001. 3(6): p. 587-95. 26. Kuratomi, G., et al., NEDD4-2 (neural precursor cell expressed, developmen-tally down-regulated 4-2) negatively regulates TGF-beta (transforming growth factor-beta) signalling by inducing ubiquitin-mediated degradation of Smad2 and TGF-beta type I receptor. Biochem J, 2005. 386(Pt 3): p. 461-70. 27. Seo, S.R., et al., The novel E3 ubiquitin ligase Tiul1 associates with TGIF to target Smad2 for degradation. EMBO J, 2004. 23(19): p. 3780-92. 28. Wan, M., et al., Jab1 antagonizes TGF-beta signaling by inducing Smad4 de-gradation. EMBO Rep, 2002. 3(2): p. 171-6. 29. Liu, P., et al., Cross talk among Smad, MAPK, and integrin signaling pathways enhances adventitial fibroblast functions activated by transforming growth factor-beta1 and inhibited by Gax. Arterioscler Thromb Vasc Biol, 2008. 28(4): p. 725-31. 30. Chen, G. and N. Khalil, TGF-beta1 increases proliferation of airway smooth muscle cells by phosphorylation of map kinases. Respir Res, 2006. 7: p. 2. 31. Pelaia, G., et al., Effects of TGF-beta and glucocorticoids on map kinase phosphorylation, IL-6/IL-11 secretion and cell proliferation in primary cultures of human lung fibroblasts. J Cell Physiol, 2007. 210(2): p. 489-97. 32. Khalil, S., et al., Dynamic regulation and involvement of the heat shock tran-scriptional response in arsenic carcinogenesis. J Cell Physiol, 2006. 207(2): p. 562-9. 33. Gallelli, L., et al., Interleukin-6 receptor superantagonist Sant7 inhibits TGF-beta-induced proliferation of human lung fibroblasts. Cell Prolif, 2008. 41(3): p. 393-407. 34. Walia, B., et al., TGF-beta down-regulates IL-6 signaling in intestinal epithelial cells: critical role of SMAD-2. Faseb J, 2003. 17(14): p. 2130-2. 35. Becker, C., et al., TGF-beta suppresses tumor progression in colon cancer by inhibition of IL-6 trans-signaling. Immunity, 2004. 21(4): p. 491-501. 36. Ratta, M., et al., Dendritic cells are functionally defective in multiple myeloma: the role of interleukin-6. Blood, 2002. 100(1): p. 230-7. 37. Cavarretta, I.T., et al., The antiapoptotic effect of IL-6 autocrine loop in a cel-lular model of advanced prostate cancer is mediated by Mcl-1. Oncogene, 2007. 26(20): p. 2822-32. 38. Blay, J.Y., et al., Serum level of interleukin 6 as a prognosis factor in metastatic renal cell carcinoma. Cancer Res, 1992. 52(12): p. 3317-22. 39. Bendicho, M.T., et al., Polymorphism of cytokine genes (TGF-beta1, IFN-gamma, IL-6, IL-10, and TNF-alpha) in patients with chronic pancreatitis. Pancreas, 2005. 30(4): p. 333-6. 40. Ishiguro, H., et al., Tumor-derived interleukin (IL)-6 induced anti-tumor effect in immune-compromised hosts. Cancer Immunol Immunother, 2005. 54(12): p. 1191-9. 41. Kamimura, D., K. Ishihara, and T. Hirano, IL-6 signal transduction and its physiological roles: the signal orchestration model. Rev Physiol Biochem Pharmacol, 2003. 149: p. 1-38. 42. Heinrich, P.C., et al., Principles of interleukin (IL)-6-type cytokine signalling and its regulation. Biochem J, 2003. 374(Pt 1): p. 1-20. 43. Wallet, M.A., P. Sen, and R. Tisch, Immunoregulation of dendritic cells. Clin Med Res, 2005. 3(3): p. 166-75. 44. Wang, Y.S., et al., Characterization of canine monocyte-derived dendritic cells with phenotypic and functional differentiation. Can J Vet Res, 2007. 71(3): p. 165-74. 45. Ronger-Savle, S., et al., TGFbeta inhibits CD1d expression on dendritic cells. J Invest Dermatol, 2005. 124(1): p. 116-8. 46. Kim, R., M. Emi, and K. Tanabe, Cancer cell immune escape and tumor pro-gression by exploitation of anti-inflammatory and pro-inflammatory responses. Cancer Biol Ther, 2005. 4(9): p. 924-33. 47. Hsiao, Y.W., et al., Interactions of host IL-6 and IFN-gamma and can-cer-derived TGF-beta1 on MHC molecule expression during tumor spontane-ous regression. Cancer Immunol Immunother, 2008. 48. Kaminrska, D., et al., [Gene expression of INF-gamma, IL-10, IL-2, IL-6, PDGF-B i TGF-beta in kidney tissue after renal transplantation]. Pol Merkur Lekarski, 2006. 21(122): p. 148-50; discussion 151. 49. Chou, C.H., et al., Up-regulation of interleukin-6 in human ovarian cancer cell via a Gi/PI3K-Akt/NF-kappaB pathway by lysophosphatidic acid, an ovarian cancer-activating factor. Carcinogenesis, 2005. 26(1): p. 45-52. 50. Chen, R.H., et al., Interleukin-6 inhibits transforming growth fac-tor-beta-induced apoptosis through the phosphatidylinositol 3-kinase/Akt and signal transducers and activators of transcription 3 pathways. J Biol Chem, 1999. 274(33): p. 23013-9. 51. Ju, X.S., et al., Transforming growth factor beta1 up-regulates interferon reg-ulatory factor 8 during dendritic cell development. Eur J Immunol, 2007. 37(5): p. 1174-83. 52. Wang, Y.S., K.H. Chi, and R.M. Chu, Cytokine profiles of canine mono-cyte-derived dendritic cells as a function of lipopolysaccharide- or tumor ne-crosis factor-alpha-induced maturation. Vet Immunol Immunopathol, 2007. 118(3-4): p. 186-98. 53. Hens, J.J., Preparation of synaptosomal plasma membranes by subcellular fractionation. Methods Mol Biol, 1997. 72: p. 61-9. 54. Kunzler, K., W. Eichenberger, and A. Radunz, Intracellular localization of two betaine lipids by cell fractionation and immunomicroscopy. Z Naturforsch [C], 1997. 52(7-8): p. 487-95. 55. Hasan, Z. and J. Pieters, Subcellular fractionation by organelle electrophoresis: separation of phagosomes containing heat-killed yeast particles. Electropho-resis, 1998. 19(7): p. 1179-84. 56. Flamand, N., et al., Phosphorylation of serine 271 on 5-lipoxygenase and its role in nuclear export. J Biol Chem, 2009. 284(1): p. 306-13. 57. Morrow, G., et al., Varicella-zoster virus productively infects mature dendritic cells and alters their immune function. J Virol, 2003. 77(8): p. 4950-9. 58. Mullen, K.M., et al., Potassium channels Kv1.3 and Kv1.5 are expressed on blood-derived dendritic cells in the central nervous system. Ann Neurol, 2006. 60(1): p. 118-27. 59. Rico, D., et al., Heavy metals generate reactive oxygen species in terrestrial and aquatic ciliated protozoa. Comp Biochem Physiol C Toxicol Pharmacol, 2009. 149(1): p. 90-96. 60. Dasgupta, S., et al., Inhibition of NK cell activity through TGF-beta 1 by down-regulation of NKG2D in a murine model of head and neck cancer. J Immunol, 2005. 175(8): p. 5541-50. 61. Dasgupta, S., et al., Recombinant vaccinia virus expressing IL-2 generates ef-fective anti-tumor responses in an orthotopic murine model of head and neck carcinoma. Mol Ther, 2003. 8(2): p. 238-48. 62. Wrana, J.L., Crossing Smads. Sci STKE, 2000. 2000(23): p. RE1. 63. Souchelnytskyi, S., et al., Phosphorylation of Smad signaling proteins by re-ceptor serine/threonine kinases. Methods Mol Biol, 2001. 124: p. 107-20. 64. Mehra, A., L. Attisano, and J.L. Wrana, Characterization of Smad phosphory-lation and Smad-receptor interaction. Methods Mol Biol, 2000. 142: p. 67-78. 65. Kersten, B., et al., Plant phosphoproteomics: a long road ahead. Proteomics, 2006. 6(20): p. 5517-28. 66. Letterio, J.J., et al., Maternal rescue of transforming growth factor-beta 1 null mice. Science, 1994. 264(5167): p. 1936-8. 67. Sporn, M.B., et al., Transforming growth factor-beta and suppression of car-cinogenesis. Princess Takamatsu Symp, 1989. 20: p. 259-66. 68. Zheng, S.G., J. Wang, and D.A. Horwitz, Cutting edge: Foxp3+CD4+CD25+ regulatory T cells induced by IL-2 and TGF-beta are resistant to Th17 conver-sion by IL-6. J Immunol, 2008. 180(11): p. 7112-6. 69. Larmonier, N., et al., Tumor-derived CD4(+)CD25(+) regulatory T cell sup-pression of dendritic cell function involves TGF-beta and IL-10. Cancer Im-munol Immunother, 2007. 56(1): p. 48-59. 70. Ohta, K., et al., IL-6 antagonizes TGF-beta and abolishes immune privilege in eyes with endotoxin-induced uveitis. Invest Ophthalmol Vis Sci, 2000. 41(9): p. 2591-9. 71. Dominitzki, S., et al., Cutting edge: trans-signaling via the soluble IL-6R ab-rogates the induction of FoxP3 in naive CD4+CD25 T cells. J Immunol, 2007. 179(4): p. 2041-5. 72. Romagnani, S., Human Th17 cells. Arthritis Res Ther, 2008. 10(2): p. 206. 73. Zhou, L., et al., TGF-beta-induced Foxp3 inhibits T(H)17 cell differentiation by antagonizing RORgammat function. Nature, 2008. 453(7192): p. 236-40. 74. Derynck, R. and Y.E. Zhang, Smad-dependent and Smad-independent path-ways in TGF-beta family signalling. Nature, 2003. 425(6958): p. 577-84. 75. Jenkins, B.J., et al., Hyperactivation of Stat3 in gp130 mutant mice promotes gastric hyperproliferation and desensitizes TGF-beta signaling. Nat Med, 2005. 11(8): p. 845-52. 76. Gabriele, L. and K. Ozato, The role of the interferon regulatory factor (IRF) family in dendritic cell development and function. Cytokine Growth Factor Rev, 2007. 18(5-6): p. 503-10. 77. Lange, C., et al., Dendritic cell-regulatory T-cell interactions control self-directed immunity. Immunol Cell Biol, 2007. 85(8): p. 575-81. 78. Hanig, J. and M.B. Lutz, Suppression of mature dendritic cell function by reg-ulatory T cells in vivo is abrogated by CD40 licensing. J Immunol, 2008. 180(3): p. 1405-13. 79. Ghiringhelli, F., et al., Tumor cells convert immature myeloid dendritic cells into TGF-beta-secreting cells inducing CD4+CD25+ regulatory T cell proliferation. J Exp Med, 2005. 202(7): p. 919-29. 80. Ouabed, A., et al., Differential control of T regulatory cell proliferation and suppressive activity by mature plasmacytoid versus conventional spleen den-dritic cells. J Immunol, 2008. 180(9): p. 5862-70. 81. Zhang, M., et al., Expression of a soluble TGF-beta receptor by tumor cells enhances dendritic cell/tumor fusion vaccine efficacy. J Immunol, 2008. 181(5): p. 3690-7. 82. Escors, D., et al., Targeting dendritic cell signaling to regulate the response to immunization. Blood, 2008. 111(6): p. 3050-61. 83. Hueman, M.T., et al., Levels of circulating regulatory CD4+CD25+ T cells are decreased in breast cancer patients after vaccination with a HER2/neu peptide (E75) and GM-CSF vaccine. Breast Cancer Res Treat, 2006. 98(1): p. 17-29. 84. Christ, O., et al., Efficacy of local versus systemic application of antibo-dy-cytokine fusion proteins in tumor therapy. Clin Cancer Res, 2001. 7(4): p. 985-98. 85. Vojinovic, S., et al., [Effects of alfacalcidol therapy on serum cytokine levels in patients with multiple sclerosis]. Srp Arh Celok Lek, 2005. 133 Suppl 2: p. 124-8. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/41685 | - |
dc.description.abstract | 在癌症病患,樹突狀細胞(DC)的功能明顯地受到抑制。而在犬傳染性花柳性的腫瘤(CTVT)模式中,當腫瘤進入自然的消退期,患犬之DC功能即恢復。已知CTVT會分泌高量的TGF-b.然而DC的功能缺失以及腫瘤進入消退期時之功能恢復,和TGF-b之間的關係以及機制上不明確。我們首先確認了CTVT分泌的TGF-b對單核球來原之C有抑制作用。給予TGF-b後,DC活化T淋巴的功能顯著的降低了,CD1a, CD40等分子的表現也減少了。以含有TGF-b之增長(P)期CTVT細胞上輕易培養DC, 也可以得到類似的效果。中和上清液中的TGF-b可以恢復DC活化T淋巴球的能力,也提高了DCMHCII的表現。實驗室之前的研究發現CTVT R期時,浸潤於腫瘤之間的淋巴球產生IL-6和TGF-b有很強的結抗效果。流是細胞儀的結果顯示IL-6可以有效的恢復DC被TGF-b抑制的MHCII表現。我們於是進一步測試IL-4是否會直接干擾TGF-b的傳導路徑。經由西方墨眼法以及共軛焦顯微鏡之觀察,我們發現IL-6會阻擋TGF-b引起之Smad2/3進入細胞核。在我們觀察IL-6減少核內Smad2/3的同時,並沒有偵測到抑制型Smad, Smad7表現而回饋控制TGF-b的傳導所致。本研究探討了宿主/癌症之間的交互關係,並提出力用IL-6回復癌症產生TGF-b 對 DC的傷害在癌症治療上的應用。 | zh_TW |
dc.description.abstract | The dendritic cell (DC) activities are significantly hampered in many cancers. It is interesting that, in a canine cancer model, canine transmissible venereal tumor (CTVT), when the cancer enters a spontaneous regression (R), the inhibited DC activities are restored. CTVT produces high levels of TGF-b However, the role of TGF-b and 5h3 mechanisms involved in the DC functional suppresion and the restoration is largely unknown. We confirmed that the CTVT-derived TGF-b suppressed monocyte-derivedDC activities. After TGF-b treatment, the T cell activation through DC was impeded and the supernatants from the progression phase CTVT that contained TGF-b. Neutralizing the TGF-b in the supernatants by specific monoclonal antibody reversed the inhibition of DC-induces lymphocyte stimulation and also enhanced the DC MHC II expression. Our precious sstudy indicated that IL-6 produces by tumor infiltrating lymphocytes in the CTVT R phase sxhibited strong anti-TGF-b activity. The recovery of the TGF-b inhibitrf DC activities by IL-6 was thereforer studied. The flow cytometry results showed a strong reaction of IL6 in restoring TGF0b0down-regulated MHC expression on DCs. We ffurther verified whether IL-6 interfered with the TGF-b activities directly. Using Western blotting and confocal microscopy, we found that the nuclear translocation of Smad2/3, a sign of signal transduction of TGF-b, was blocked by adding IL-6. The evidence that the Smad7, which is an inhibitory Smad, was not oncreased in expression by adding IL-6 indicated that the nuclear translocation of Smad2/3 blocked by IL-6 was not through Smad7 pathway. This study provides in depth understanding of the host/cancer interactions and possible applications of IL-6 to restore DC activities in cancers that produce TGF-b. | en |
dc.description.provenance | Made available in DSpace on 2021-06-15T00:27:35Z (GMT). No. of bitstreams: 1 ntu-98-R95629005-1.pdf: 2212278 bytes, checksum: 62a3abcf970b36564d5fc630030b92a8 (MD5) Previous issue date: 2009 | en |
dc.description.tableofcontents | 口試委員審定書………………………………………………………………………I
誌謝………………………………………………………………………………..…. .I 中文摘要………………………………………………………………………………II Abstract ……………………………………………………………………………....III Contents……………………………………………………………………………....IV Abbriviation………………………………………………………………………….VI Chapter 1. Background and Literature Review 1 1.1Dendritic cells 1 1.1.1 Subpopulations of dendritic cells 1 1.1.2 Surface molecule expression 2 1.1.3 Alterations in phenotype and functions caused by tumor 4 1.2 Canine transmissible venereal tumor 5 1.3 TGF-β 5 1.3.1 Effects of TGF-βin tumor immunology 6 1.3.2 TGF-β signaling 7 1.3.2.1 Smad-dependent pathway 7 1.3.2.2 Smad-independent pathway 10 1.3.2.3 Cross talk of Smads and other compounds 12 1.4 IL-6 ………………………………………………………………………..12 1.4.1 Pro tumoral activities of IL-6 13 1.4.2 Anti-tumoral activities of IL-6 13 1.4.3 Signaling pathway of IL-6 14 1.5 Objectives of this study 15 Chapter 2. Introduction 16 Chapter 3. Materials and Methods 19 3.1 Animals and generation of peripheral blood-derived DC 19 3.2 Flow cytometry analysis of DC phenotypes 19 3.3 Real-time RT-PCR 21 3.4 Allogeneic Mix Lymphocyte Reaction (MLR) 22 3.5 FITC-dextran uptake assay 22 3.6 Production of CTVT cell-culture supernatants 23 3.7 Cell fractionation and Western immunoblotting 23 3.8 Immunofluorescent staining for confocal microscopy 24 3.9 Enzyme-linked immunosorbant assay ( ELISA) 25 3.10 Statistical analysis 25 Chapter 4. Results 26 Chapter 1. Background and Literature Review 1 1.1Dendritic cells 1 1.1.1 Subpopulations of dendritic cells 1 1.1.2 Surface molecule expression 2 1.1.3 Alterations in phenotype and functions caused by tumor 4 1.2 Canine transmissible venereal tumor 5 1.3 TGF-β 5 1.3.1 Effects of TGF-βin tumor immunology 6 1.3.2 TGF-β signaling 7 1.3.2.1 Smad-dependent pathway 7 1.3.2.2 Smad-independent pathway 10 1.3.2.3 Cross talk of Smads and other compounds 12 1.4 IL-6 ………………………………………………………………………..12 1.4.1 Pro tumoral activities of IL-6 13 1.4.2 Anti-tumoral activities of IL-6 13 1.4.3 Signaling pathway of IL-6 14 1.5 Objectives of this study 15 Chapter 2. Introduction 16 Chapter 3. Materials and Methods 19 3.1 Animals and generation of peripheral blood-derived DC 19 3.2 Flow cytometry analysis of DC phenotypes 19 3.3 Real-time RT-PCR 21 3.4 Allogeneic Mix Lymphocyte Reaction (MLR) 22 3.5 FITC-dextran uptake assay 22 3.6 Production of CTVT cell-culture supernatants 23 3.7 Cell fractionation and Western immunoblotting 23 3.8 Immunofluorescent staining for confocal microscopy 24 3.9 Enzyme-linked immunosorbant assay ( ELISA) 25 3.10 Statistical analysis 25 Chapter 4. Results 26 4.1 TGF-β effects on DC 26 4.1.1 Phenotypic changes of canine DC 26 4.1.2 Inhibition of DC function 29 4.2 Recovery of TGF-β-suppressed activities 32 4.3 IL-6 effects on Smad2/3 TGF-β signaling 34 4.4 IL-6 effects on Smad2/3 nuclear translocation not by the induction of Smad7 ……………………………………………………………………….40 Chapter 5. Discussion 41 References 46 | |
dc.language.iso | en | |
dc.title | IL-6 經由Smad恢復TGF-beta對樹狀細胞成熟的抑制 | zh_TW |
dc.title | IL-6 Antagonizes the Inhibitory Activities of TGF-beta on Dendritic Cell Maturation via Smads | en |
dc.type | Thesis | |
dc.date.schoolyear | 97-1 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 郭明良,季匡華,詹東榮,廖光文 | |
dc.subject.keyword | 單核球來源樹突狀細胞,犬傳染性花柳病,Smads,IL-6,TGF-beta, | zh_TW |
dc.subject.keyword | Monocyte-derived-DC,CTVT,Smads,IL-6,TGF-beta, | en |
dc.relation.page | 51 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2009-01-21 | |
dc.contributor.author-college | 獸醫專業學院 | zh_TW |
dc.contributor.author-dept | 獸醫學研究所 | zh_TW |
顯示於系所單位: | 獸醫學系 |
文件中的檔案:
檔案 | 大小 | 格式 | |
---|---|---|---|
ntu-98-1.pdf 目前未授權公開取用 | 2.16 MB | Adobe PDF |
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。