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標題: | 甘藷中受傷相關NAC基因家族成員之分子選殖與功能分析 Molecular cloning and functional characterization of wound-inducible NAC genes in sweet potato (Ipomoea batatas cv. Tainong 57) |
作者: | Dan-Wen Hsieh 謝丹文 |
指導教授: | 葉開溫 |
關鍵字: | NAC domain,逆境,甘藷 (台農57),基因表現,cDNA 基因庫, NAC domain,stress,sweet potato (Ipomoea batatas cv. Tainong 57),gene expression,cDNA library, |
出版年 : | 2009 |
學位: | 碩士 |
摘要: | Abiotic and biotic stresses cause major losses in crop productivity worldwide. Multiple signaling pathways regulate the stress responses in plants. Transcription factors are one of the groups that are involved in the regulation of signal transduction and gene expression. NAC family is composed of plant-specific transcription factors, and contains 75 and 105 members in rice and Arabidopsis, respectively. We are interested in the NAC genes in sweet potatos (Ipomoea batatas cv. Tainong 57) that may participate in response to wounding, and isolated three sweet potato NAC genes from a phage cDNA library. These genes belong to an ATAF subfamily, which contains genes that are responsive to defenses and stresses. The cDNA and protein sequences of the three genes are highly similar to each other. The cDNA sequences of IbNAC1-1 and IbNAC2-18 contain 900 bp open reading frame (ORF), which encode proteins of 300 amino acids, and include the five conserved blocks of homology that characterize the NAC family. IbNAC97 contains 696 bp ORF, which encodes a protein of 232 amino acids, but only contains three conserved blocks of NAC domain. The C-termini of three proteins are not various, but are very similar. The Southern blotting analysis revealed that there were multiple members in sweet potato NAC family. The gene expression pattern showed that the three genes are abundant in root and tuber, and could be induced by wounding and lots of stress treatments. However, the expression level of IbNAC97 was low. After wounding, the three genes were induced at highest level within thirty minutes, and extended to six hours. Drought and high salinity could induce three genes within thirty minutes, and extended to twelve hours. The cold treatment induced latent expression of three genes. IbNAC97 was much more induced by cold treatment than other stress treatments. Three genes were induced instantly by MeJA, and for a moment by ABA. SA induced gene expressions in thirty minutes and to two hours. The IbNAC1-overexpression Arabidopsis plants were more sensitive to ABA and high salinity, flowered earlier than wild-type plants, and had abnormal flower structure. In conclusion, the three NAC genes reported here are all stress-induced ones in NAC gene family and may be involved in stress signal transduction pathways. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/41540 |
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顯示於系所單位: | 植物科學研究所 |
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