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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 李後晶 | |
| dc.contributor.author | I-Cheng Cheng | en |
| dc.contributor.author | 陳易呈 | zh_TW |
| dc.date.accessioned | 2021-06-15T00:18:40Z | - |
| dc.date.available | 2012-03-23 | |
| dc.date.copyright | 2009-03-23 | |
| dc.date.issued | 2009 | |
| dc.date.submitted | 2009-03-09 | |
| dc.identifier.citation | References
Abraham, N. G., Y. Lavrovsky, M. L. Schwartzman, R. A. Stoltz, R. D. Levere, M. E. Gerritsen, S. Shibahara, and A. Kappas. 1995. Transfection of the human heme oxygenase gene into rabbit coronary microvessel endothelial cells: protective effect against heme and hemoglobin toxicity. Proc. Natl. Acad. Sci. USA. 92: 6798-6802. Akyol, A., H. Ulusoy, M. Imamoglu, A. Cay, E. Yulug, A. Alver, E. Erturk, M. Kosucu, A. Besir, A. Akyol, and I. Ozen. 2006. Does propofol or caffeic acid phenethyl ester prevent lung injury after hindlimb ischaemia-reperfusion in ventilated rats? Injury 37: 380-387. Applegate, L. A., P. Luscher, and R. M. Tyrrell. 1991. Induction of heme oxygenase: a general response to oxidant stress in cultured mammalian cells. Cancer Res. 51: 974-978. Azam, S., N. Hadi, N. U. Khan, and S. M. Hadi. 2004. Prooxidant property of green tea polyphenols epicatechin and epigallocatechin-3-gallate: implications for anticancer properties. Toxicol. In Vitro 18: 555-561. Balogun, E., M. Hoque, P. Gong, E. Killeen, C. J. Green, R. Foresti, J. Alam, and R. Motterlini. 2003. Curcumin activates the haem oxygenase-1 gene via regulation of Nrf2 and the antioxidant-responsive element. Biochem. J. 371: 887-895. Bankova, V., R. Christov, A. Kujumgiev, M. C. Marcucci, and S. Popov. 1995. Chemical composition and antibacterial activity of Brazilian propolis. Z. Naturforsch [C] 50: 167-172. Banskota, A. H., Y. Tezuka, and S. Kadota. 2001. Recent progress in pharmacological research of propolis. Phytother. Res. 15: 561-571. Bhat, S. H., A. S. Azmi, and S. M. Hadi. 2007. Prooxidant DNA breakage induced by caffeic acid in human peripheral lymphocytes: involvement of endogenous copper and a putative mechanism for anticancer properties. Toxicol. Appl. Pharmacol. 218: 249-255. Bhimani, R. S., W. Troll, D. Grunberger, and K. Frenkel. 1993. Inhibition of oxidative stress in HeLa cells by chemopreventive agents. Cancer Res. 53: 4528-4533. Burdock, G. A. 1998. Review of the biological properties and toxicity of bee propolis (propolis). Food Chem. Toxicol. 36: 347-363. Cagli, K., C. Bagci, M. Gulec, B. Cengiz, O. Akyol, I. Sari, S. Cavdar, S. Pence, and H. Dinckan. 2005. In vivo effects of caffeic acid phenethyl ester on myocardial ischemia-reperfusion injury and apoptotic changes in rats. Ann. Clin. Lab. Sci. 35: 440-448. Castaldo, S. and F. Capasso. 2002. Propolis, an old remedy used in modern medicine. Fitoterapia 73 Suppl 1: S1-6. Celli, N., L. K. Dragani, S. Murzilli, T. Pagliani, and A. Poggi. 2007. In vitro and in vivo stability of caffeic acid phenethyl ester, a bioactive compound of propolis. J. Agric. Food Chem. 55: 3398-3407. Cemeli, E., A. Baumgartner, and D. Anderson. 2009. Antioxidants and the Comet assay. Mutat. Res. 681: 51-67. Chang, M. J. 1991. UV absorption as an approximation for cell number in in vitro toxicity testing. Bull. Environ. Contam. Toxicol. 47: 190-197. Chen, M. J., W. H. Chang, C. C. Lin, C. Y. Liu, T. E. Wang, C. H. Chu, S. C. Shih, and Y. J. Chen. 2008. Caffeic acid phenethyl ester induces apoptosis of human pancreatic cancer cells involving caspase and mitochondrial dysfunction. Pancreatology 8: 566-576. Chen, Y. J., M. S. Shiao, and S. Y. Wang. 2001a. The antioxidant caffeic acid phenethyl ester induces apoptosis associated with selective scavenging of hydrogen peroxide in human leukemic HL-60 cells. Anticancer Drugs 12: 143-149. Chen, Y. J., M. S. Shiao, M. L. Hsu, T. H. Tsai, and S. Y. Wang. 2001b. Effect of caffeic acid phenethyl ester, an antioxidant from propolis, on inducing apoptosis in human leukemic HL-60 cells. J. Agric. Food Chem. 49: 5615-5619. Chiao, C., A. M. Carothers, D. Grunberger, G. Solomon, G. A. Preston, and J. C. Barrett. 1995. Apoptosis and altered redox state induced by caffeic acid phenethyl ester (CAPE) in transformed rat fibroblast cells. Cancer Res. 55: 3576-3583. Chung, M. J., P. A. Walker, and C. Hogstrand. 2006. Dietary phenolic antioxidants, caffeic acid and Trolox, protect rainbow trout gill cells from nitric oxide-induced apoptosis. Aquat. Toxicol. 80: 321-328. da Cunha, F. M., D. Duma, J. Assreuy, F. C. Buzzi, R. Niero, M. M. Campos, and J. B. Calixto. 2004. Caffeic acid derivatives: in vitro and in vivo anti-inflammatory properties. Free Radic. Res. 38: 1241-1253. Frankel, D., K. Mehindate, and H. M. Schipper. 2000. Role of heme oxygenase-1 in the regulation of manganese superoxide dismutase gene expression in oxidatively-challenged astroglia. J. Cell Physiol. 185: 80-86. Furukawa, A., S. Oikawa, M. Murata, Y. Hiraku, and S. Kawanishi. 2003. (-)-Epigallocatechin gallate causes oxidative damage to isolated and cellular DNA. Biochem. Pharmacol. 66: 1769-1778. Ghisalberti, E. L. 1979. Propolis: a review. Bee World 60: 59-84. Grunberger, D., R. Banerjee, K. Eisinger, E. M. Oltz, L. Efros, M. Caldwell, V. Estevez, and K. Nakanishi. 1988. Preferential cytotoxicity on tumor cells by caffeic acid phenethyl ester isolated from propolis. Experientia 44: 230-232. Guarini, L., Z. Z. Su, S. Zucker, J. Lin, D. Grunberger, and P. B. Fisher. 1992. Growth inhibition and modulation of antigenic phenotype in human melanoma and glioblastoma multiforme cells by caffeic acid phenethyl ester (CAPE). Cell Mol. Biol. 38: 513-527. Halliwell, B. and J. M. Gutteridge. 1990. Role of free radicals and catalytic metal ions in human disease: an overview. Methods Enzymol. 186: 1-85. Hay, K. D. and D. E. Greig. 1990. Propolis allergy: a cause of oral mucositis with ulceration. Oral Surg. Oral Med. Oral Pathol. 70: 584-586. He, Y. J., B. H. Liu, D. B. Xiang, Z. Y. Qiao, T. Fu, and Y. H. He. 2006. Inhibitory effect of caffeic acid phenethyl ester on the growth of SW480 colorectal tumor cells involves beta-catenin associated signaling pathway down-regulation. World J. Gastroenterol. 12: 4981-4985. Hung, M. W., M. S. Shiao, L. C. Tsai, G. G. Chang, and T. C. Chang. 2003. Apoptotic effect of caffeic acid phenethyl ester and its ester and amide analogues in human cervical cancer ME180 cells. Anticancer Res. 23: 4773-4780. Iraz, M., E. Ozerol, M. Gulec, S. Tasdemir, N. Idiz, E. Fadillioglu, M. Naziroglu, and O. Akyol. 2006. Protective effect of caffeic acid phenethyl ester (CAPE) administration on cisplatin-induced oxidative damage to liver in rat. Cell Biochem. Funct. 24: 357-361. Jaiswal, A. K., R. Venugopal, J. Mucha, A. M. Carothers, and D. Grunberger. 1997. Caffeic acid phenethyl ester stimulates human antioxidant response element-mediated expression of the NAD(P)H:quinone oxidoreductase (NQO1) gene. Cancer Res. 57: 440-446. Jehnes, S., G. Betz, G. Bahnweg, K. Haberer, H. Sandermann, and H. Rennenberg. 2007. Tree internal signalling and defence reactions under ozone exposure in sun and shade leaves of European beech (Fagus sylvatica L.) trees. Plant Biol. (Stuttg) 9: 253-264. Jin, U. H., K. H. Song, M. Motomura, I. Suzuki, Y. H. Gu, Y. J. Kang, T. C. Moon, and C. H. Kim. 2008. Caffeic acid phenethyl ester induces mitochondria-mediated apoptosis in human myeloid leukemia U937 cells. Mol. Cell. Biochem. 310: 43-48. Johnson, M. K. and G. Loo. 2000. Effects of epigallocatechin gallate and quercetin on oxidative damage to cellular DNA. Mutat. Res. 459: 211-218. Jung, U. J., M. K. Lee, Y. B. Park, S. M. Jeon, and M. S. Choi. 2006. Antihyperglycemic and antioxidant properties of caffeic acid in db/db mice. J. Pharmacol. Exp. Ther. 318: 476-483. Jung, W. K., D. Y. Lee, Y. H. Choi, S. S. Yea, I. Choi, S. G. Park, S. K. Seo, S. W. Lee, C. M. Lee, S. K. Kim, Y. J. Jeon, and I. W. Choi. 2008. Caffeic acid phenethyl ester attenuates allergic airway inflammation and hyperresponsiveness in murine model of ovalbumin-induced asthma. Life Sci. 82: 797-805. Kanadzu, M., Y. Lu, and K. Morimoto. 2006. Dual function of (--)-epigallocatechin gallate (EGCG) in healthy human lymphocytes. Cancer Lett. 241: 250-255. Khanduja, K. L., P. K. Avti, S. Kumar, N. Mittal, K. K. Sohi, and C. M. Pathak. 2006. Anti-apoptotic activity of caffeic acid, ellagic acid and ferulic acid in normal human peripheral blood mononuclear cells: a Bcl-2 independent mechanism. Biochim. Biophys. Acta. 1760: 283-289. Kirkby, K. A. and C. A. Adin. 2006. Products of heme oxygenase and their potential therapeutic applications. Am. J. Physiol. Renal Physiol. 290: F563-571. Kumamoto, M., T. Sonda, K. Nagayama, and M. Tabata. 2001. Effects of pH and metal ions on antioxidative activities of catechins. Biosci. Biotechnol. Biochem. 65: 126-132. Kvam, E., V. Hejmadi, S. Ryter, C. Pourzand, and R. M. Tyrrell. 2000. Heme oxygenase activity causes transient hypersensitivity to oxidative ultraviolet A radiation that depends on release of iron from heme. Free Radic. Biol. Med. 28: 1191-1196. Lee, Y. Y., C. L. Kao, P. H. Tsai, T. H. Tsai, S. H. Chiou, W. F. Wu, H. H. Ku, and T. T. Wong. 2008. Caffeic acid phenethyl ester preferentially enhanced radiosensitizing and increased oxidative stress in medulloblastoma cell line. Childs Nerv. Syst. 24: 987-994. Llesuy, S. F. and M. L. Tomaro. 1994. Heme oxygenase and oxidative stress. Evidence of involvement of bilirubin as physiological protector against oxidative damage. Biochim. Biophys. Acta. 1223: 9-14. Maruhashi, K., Y. Kasahara, K. Ohta, T. Wada, K. Ohta, N. Nakamura, T. Toma, S. Koizumi, and A. Yachie. 2004. Paradoxical enhancement of oxidative cell injury by overexpression of heme oxygenase-1 in an anchorage-dependent cell ECV304. J. Cell Biochem. 93: 552-562. Menniti-Ippolito, F., G. Mazzanti, A. Vitalone, F. Firenzuoli, and C. Santuccio. 2008. Surveillance of suspected adverse reactions to natural health products: the case of propolis. Drug Saf. 31: 419-423. Michaluart, P., J. L. Masferrer, A. M. Carothers, K. Subbaramaiah, B. S. Zweifel, C. Koboldt, J. R. Mestre, D. Grunberger, P. G. Sacks, T. Tanabe, and A. J. Dannenberg. 1999. Inhibitory effects of caffeic acid phenethyl ester on the activity and expression of cyclooxygenase-2 in human oral epithelial cells and in a rat model of inflammation. Cancer Res. 59: 2347-2352. Mirzoeva, O. K., G. F. Sud'ina, M. A. Pushkareva, G. A. Korshunova, N. V. Sumbatian, and S. D. Varfolomeev. 1995. [Lipophilic derivatives of caffeic acid as lipoxygenase inhibitors with antioxidant properties]. Bioorg. Khim. 21: 143-151. Nagaoka, T., A. H. Banskota, Y. Tezuka, Y. Harimaya, K. Koizumi, I. Saiki, and S. Kadota. 2003. Inhibitory effects of caffeic acid phenethyl ester analogues on experimental lung metastasis of murine colon 26-L5 carcinoma cells. Biol. Pharm. Bull. 26: 638-641. Nagaoka, T., A. H. Banskota, Y. Tezuka, I. Saiki, and S. Kadota. 2002. Selective antiproliferative activity of caffeic acid phenethyl ester analogues on highly liver-metastatic murine colon 26-L5 carcinoma cell line. Bioorg. Med. Chem. 10: 3351-3359. Nakagami, T., K. Toyomura, T. Kinoshita, and S. Morisawa. 1993. A beneficial role of bile pigments as an endogenous tissue protector: anti-complement effects of biliverdin and conjugated bilirubin. Biochim. Biophys. Acta. 1158: 189-193. Nakagawa, H., K. Hasumi, J. T. Woo, K. Nagai, and M. Wachi. 2004. Generation of hydrogen peroxide primarily contributes to the induction of Fe(II)-dependent apoptosis in Jurkat cells by (-)-epigallocatechin gallate. Carcinogenesis 25: 1567-1574. Nardini, M., P. Pisu, V. Gentili, F. Natella, M. Di Felice, E. Piccolella, and C. Scaccini. 1998. Effect of caffeic acid on tert-butyl hydroperoxide-induced oxidative stress in U937. Free Radic. Biol. Med. 25: 1098-1105. Natarajan, K., S. Singh, T. R. Burke, Jr., D. Grunberger, and B. B. Aggarwal. 1996. Caffeic acid phenethyl ester is a potent and specific inhibitor of activation of nuclear transcription factor NF-kappa B. Proc. Natl. Acad. Sci. USA. 93: 9090-9095. Olive, P. L. 2004. Detection of DNA damage in individual cells by analysis of histone H2AX phosphorylation. Methods Cell. Biol. 75: 355-373. Ozen, S., O. Akyol, M. Iraz, S. Sogut, F. Ozugurlu, H. Ozyurt, E. Odaci, and Z. Yildirim. 2004. Role of caffeic acid phenethyl ester, an active component of propolis, against cisplatin-induced nephrotoxicity in rats. J. Appl. Toxicol. 24: 27-35. Paull, T. T., E. P. Rogakou, V. Yamazaki, C. U. Kirchgessner, M. Gellert, and W. M. Bonner. 2000. A critical role for histone H2AX in recruitment of repair factors to nuclear foci after DNA damage. Curr. Biol. 10: 886-895. Raza, H. and A. John. 2005. Green tea polyphenol epigallocatechin-3-gallate differentially modulates oxidative stress in PC12 cell compartments. Toxicol. Appl. Pharmacol. 207: 212-220. Rezzani, R., L. Giugno, B. Buffoli, F. Bonomini, and R. Bianchi. 2005. The protective effect of caffeic acid phenethyl ester against cyclosporine A-induced cardiotoxicity in rats. Toxicology 212: 155-164. Rogakou, E. P., C. Boon, C. Redon, and W. M. Bonner. 1999. Megabase chromatin domains involved in DNA double-strand breaks in vivo. J. Cell Biol. 146: 905-916. Rossi, A., A. Ligresti, R. Longo, A. Russo, F. Borrelli, and L. Sautebin. 2002. The inhibitory effect of propolis and caffeic acid phenethyl ester on cyclooxygenase activity in J774 macrophages. Phytomedicine 9: 530-535. Ryter, S. W., J. Alam, and A. M. Choi. 2006. Heme oxygenase-1/carbon monoxide: from basic science to therapeutic applications. Physiol. Rev. 86: 583-650. Sahin, O., O. Sulak, Y. Yavuz, E. Uz, I. Eren, H. Ramazan Yilmaz, M. A. Malas, I. Altuntas, and A. Songur. 2006. Lithium-induced lung toxicity in rats: the effect of caffeic acid phenethyl ester (CAPE). Pathology 38: 58-62. Sakagami, H., H. Arakawa, M. Maeda, K. Satoh, T. Kadofuku, K. Fukuchi, and K. Gomi. 2001. Production of hydrogen peroxide and methionine sulfoxide by epigallocatechin gallate and antioxidants. Anticancer Res. 21: 2633-2641. Scapagnini, G., R. Foresti, V. Calabrese, A. M. Giuffrida Stella, C. J. Green, and R. Motterlini. 2002. Caffeic acid phenethyl ester and curcumin: a novel class of heme oxygenase-1 inducers. Mol. Pharmacol. 61: 554-561. Stocker, R., Y. Yamamoto, A. F. McDonagh, A. N. Glazer, and B. N. Ames. 1987. Bilirubin is an antioxidant of possible physiological importance. Science 235: 1043-1046. Sud'ina, G. F., O. K. Mirzoeva, M. A. Pushkareva, G. A. Korshunova, N. V. Sumbatyan, and S. D. Varfolomeev. 1993. Caffeic acid phenethyl ester as a lipoxygenase inhibitor with antioxidant properties. FEBS. Lett. 329: 21-24. Suttner, D. M. and P. A. Dennery. 1999. Reversal of HO-1 related cytoprotection with increased expression is due to reactive iron. Faseb. J. 13: 1800-1809. Suzuki, K., I. Tanaka, I. Nakanishi, A. Kurematsu, H. Yakumaru, N. Ikota, and H. Ishihara. 2006. Drastic effect of several caffeic acid derivatives on the induction of heme oxygenase-1 expression revealed by quantitative real-time RT-PCR. Biofactors 28: 151-158. Tavares, D. C., G. R. Mazzaron Barcelos, L. F. Silva, C. C. Chacon Tonin, and J. K. Bastos. 2006. Propolis-induced genotoxicity and antigenotoxicity in Chinese hamster ovary cells. Toxicol. In Vitro 20: 1154-1158. Tian, B., Z. Sun, Z. Xu, and Y. Hua. 2007. Chemiluminescence analysis of the prooxidant and antioxidant effects of epigallocatechin-3-gallate. Asia Pac. J. Clin. Nutr. 16 Suppl 1: 153-157. Usia, T., A. H. Banskota, Y. Tezuka, K. Midorikawa, K. Matsushige, and S. Kadota. 2002. Constituents of Chinese propolis and their antiproliferative activities. J. Nat. Prod. 65: 673-676. Wang, D., D. B. Xiang, Y. J. He, Z. P. Li, X. H. Wu, J. H. Mou, H. L. Xiao, and Q. H. Zhang. 2005. Effect of caffeic acid phenethyl ester on proliferation and apoptosis of colorectal cancer cells in vitro. World J. Gastroenterol. 11: 4008-4012. Watabe, M., K. Hishikawa, A. Takayanagi, N. Shimizu, and T. Nakaki. 2004. Caffeic acid phenethyl ester induces apoptosis by inhibition of NFkappaB and activation of Fas in human breast cancer MCF-7 cells. J. Biol. Chem. 279: 6017-6026. Yamamoto, T., S. Hsu, J. Lewis, J. Wataha, D. Dickinson, B. Singh, W. B. Bollag, P. Lockwood, E. Ueta, T. Osaki, and G. Schuster. 2003. Green tea polyphenol causes differential oxidative environments in tumor versus normal epithelial cells. J. Pharmacol. Exp. Ther. 307: 230-236. Zheng, L. F., F. Dai, B. Zhou, L. Yang, and Z. L. Liu. 2008. Prooxidant activity of hydroxycinnamic acids on DNA damage in the presence of Cu(II) ions: mechanism and structure-activity relationship. Food Chem. Toxicol. 46: 149-156. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/41416 | - |
| dc.description.abstract | 抗氧化功能被廣泛認為是蜂膠的眾多藥理作用之一，在世界各地及台灣的各類蜂膠萃取物同樣也展現抗氧化的能力。例如咖啡酸酚酯 (caffeic acid phenethyl ester，簡稱CAPE) 無論在細胞培養或活體實驗中，其均可降低細胞因自由基過載所導致的氧化傷害。不過我們卻發現CAPE本身除了抗氧化功能外，亦會促使自由基的產生。分析結果顯示CAPE在早期具有抗氧化的功能；在晚期則會反轉為促氧化的角色，這樣的現象跟處理時間有密切的關聯，而無關於劑量。進一步釐清其中的機制，我們發現CAPE會誘發抗氧化蛋白heme-oxygenase 1 (HO-1) 的表現，此可解釋CAPE抗氧化的現象，但在晚期，CAPE所引起HO-1的表現則呈現過量狀態，而促使細胞鐵離子濃度失衡，過多的游離鐵離子易與過氧化氫作用而生成具有高度破壞性的氫氧自由基，進而造成氧化壓力與細胞凋亡。當HO-1的活性被專一擷抗劑抑制後，我們不僅發現CAPE在晚期所造成細胞毒性與細胞凋亡減低，其同時也遮蔽了CAPE抗氧化及促氧化的雙重效應，由此可見HO-1確實在CAPE對於細胞自由基的載覆性扮演重要的角色。雖然CAPE晚期促氧化的現象可能具有潛在風險，但是此促氧化的特質可誘使NB4血癌細胞由粒線體的途徑進行細胞凋亡，其機制涉及抗細胞凋亡Bcl-2家族蛋白Bcl-2及Mcl-1的抑制；以及提升同家族蛋白促細胞凋亡的Bmf、Noxa和Bax之表現，進而導致粒線體膜電位差的喪失及Cytochrome c的溢漏，相繼造成Caspase-9與Caspase-3的活化造成細胞凋亡現象，最後而達到抑制癌細胞生長的目的。本論文評估CAPE具有癌症臨床醫療的可能性，而利用蜂膠或CAPE於保健上的應用亦需謹慎。 | zh_TW |
| dc.description.abstract | Caffeic acid phenethyl ester (CAPE), an active component from bee propolis, has been recognized as a strong antioxidant agent. However, several previous documents report the pro-oxidant effect of CAPE. In this investigation to specify the role of CAPE on intracellular intracellular reactive oxygen species (ROS), we report CAPE acts either as a protective antioxidant at early phase of treatment or a deleterious pro-oxidant during late time period to human promyelocytic NB4 cells. This double-edged effect of CAPE depended on the treatment time phase rather than the dose. Short-term CAPE treatment was protective due to the induction of heme-oxygenase-1 (HO-1) at an appropriate level which catalyzed anti-oxidative heme degradation. Long-term CAPE treatment was deleterious because overexpression of HO-1 led to inhibition of ferritin and thereby the transient existence of ferrous iron, which in turn reacted with hydrogen peroxide through the Fenton reaction to produce more-harmful hydroxyl radicals. The pro-oxidative nature also is involved in CAPE-induced typical mitochondrion-dependent apoptosis. The molecular mechanism of CAPE-driven apoptosis is associated with multiple Bcl-2 family proteins including inhibition of Bcl-2 and Mcl-1 and up-regulation of Bmf, Noxa, and Bax. The results of interaction of those Bcl-2 family proteins cause loss of mitochondrial membrane potential and leaking of Cytochrome c, and subsequently activate Caspase-9 and Caspase -3 to complete the intrinsic apoptotic signal finally. The conclusion of this dissertation suggests that CAPE (or propolis containing CAPE) could be a potential candidate for cancer therapy in the clinic and should be applied carefully. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-15T00:18:40Z (GMT). No. of bitstreams: 1 ntu-98-F92632004-1.pdf: 1146757 bytes, checksum: dd8a91f185dfb6055faa7a18e9872c3b (MD5) Previous issue date: 2009 | en |
| dc.description.tableofcontents | 口試委員審定書 ---------------------------------------------------------------------------------- i
誌謝 ------------------------------------------------------------------------------------------------ ii 摘要及關鍵詞 ----------------------------------------------------------------------------------- iii Abstract and key words -------------------------------------------------------------------------- v Introduction ---------------------------------------------------------------------------------------- 1 Materials and methods ---------------------------------------------------------------------------- 4 Chemicals ------------------------------------------------------------------------------------ 4 Cells ------------------------------------------------------------------------------------------- 4 Assay of cell growth ------------------------------------------------------------------------ 5 Analysis of intracellular oxidative stress ------------------------------------------------- 5 DNA damage assay by measuring γH2AX intensity ----------------------------------- 6 Immunoblotting ----------------------------------------------------------------------------- 6 Releasing of cytochrome c ----------------------------------------------------------------- 7 Apoptosis assay ----------------------------------------------------------------------------- 7 Analysis of mitochondrial membrane potential ----------------------------------------- 8 Results ---------------------------------------------------------------------------------------------- 8 Biphasic effect of CAPE on intracellular H2O2 induction and cell growth in NB4 cells ------------------------------------------------------------------------------------------- 8 CAPE-caused growth inhibition is associated with apoptosis ----------------------- 10 Antioxidant treatment buffered CAPE-caused growth inhibition and apoptosis -- 11 Double-edge role of CAPE in intracellular ROS regulation in NB4 cells --------- 11 HO-1 plays a key role in the CAPE-induced double-edge effect on cellular ROS alterations ----------------------------------------------------------------------------------- 16 CAPE perturbed iron homeostasis ------------------------------------------------------ 18 Pro-oxidant role of CAPE drives NB4 cells to apoptosis via mitochondrial pathway ------------------------------------------------------------------------------------------------ 20 Discussion ---------------------------------------------------------------------------------------- 21 Figures -------------------------------------------------------------------------------------------- 27 References ---------------------------------------------------------------------------------------- 48 | |
| dc.language.iso | en | |
| dc.title | 蜂膠萃取物咖啡酸酚酯對血癌細胞氧化自由基與細胞凋亡的雙刃效應 | zh_TW |
| dc.title | Double-edge Effect of Caffeic Acid Phenethyl Ester (CAPE) on Intracellular Reactive Oxygen Species and Apoptosis in NB4 Leukemia Cells | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 97-1 | |
| dc.description.degree | 博士 | |
| dc.contributor.coadvisor | 王清澄 | |
| dc.contributor.oralexamcommittee | 石正人,張俊哲,楊恩誠,路光暉 | |
| dc.subject.keyword | 蜂膠,咖啡酸酚酯,氧化自由基,細胞凋亡,血癌細胞, | zh_TW |
| dc.subject.keyword | propolis,caffeic acid phenethyl ester,reactive oxygen species,apoptosis,leukemia, | en |
| dc.relation.page | 60 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2009-03-10 | |
| dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
| dc.contributor.author-dept | 昆蟲學研究所 | zh_TW |
| 顯示於系所單位： | 昆蟲學系 | |
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