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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
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dc.contributor.advisor | 陳俊宏(Jiun-Hong Chen) | |
dc.contributor.author | Bin-Yu Li | en |
dc.contributor.author | 李彬煜 | zh_TW |
dc.date.accessioned | 2021-06-14T17:21:02Z | - |
dc.date.available | 2014-08-15 | |
dc.date.copyright | 2011-08-15 | |
dc.date.issued | 2011 | |
dc.date.submitted | 2011-08-11 | |
dc.identifier.citation | Ahmed S, Kozma R, Lee J, Monfries C, Harden N and Lim L. (1991) The cysteine-rich domain of human proteins, neuronal chimaerin, protein kinase C and diacylglycerol kinase binds zinc. Evidence for the involvement of a zinc-dependent structure in phorbol ester binding. Journal of Biochemistry 280 (1): 233-41
Bell, R. M. and Parker, P. J. (1991) Lipid activation of protein kinase C. Journal of Biological Chemistry 266 (8): 4661-4 Beyersmann, D., Block, C., and Malviya, A.N. (1994) Effects of Cadmium on Nuclear Protein Kinase C Environmental Health Perspect 102 (3): 177-80 Beyersmann, D. and Hechtenberg, S. (1997) Cadmium, generegulation, and cellular signaling in mammalian cells. Toxicology and Applied Pharmacology 144: 247-61 Brulle, F., Mitta, G., Cocquerelle, C., Vieau, D., Lemiere, S., Lepretre, A. and Vandenbulcke, F. (2006) Cloning and real-time PCR testing of 14 potential biomarkers in Eisenia fetida following cadmium exposure. Environmental Science & Technology 40: 2844-50 Coussens, L., Parker, P. J., Rhee, L., Yang-Feng, T. L., Chen, E., Waterfield, M. D., Francke, I. and Illrich, A. (1986) Multiple, distinct forms of bovine and human protein kinase C suggest diversity in cellular signaling pathways. Science 233: 859-66 Forbes, I.J., Zalewski, P.D., Giannakis, C., Petkoff, H.S. and Cowled, P.A. (1990) Interaction between protein kinase C and regulatory ligand is enhanced by a chelatable pool of cellular zinc. Biochimica et Biophysica Acta 1053: 113-7 Gourgou, E., Aggeli, I.-K., Beis, I. and Gaitanaki, C. (2010) Hyperthermia-induced Hsp70 and MT20 transcriptional up-regulation are mediated by p38-MAPK and JNKs in Mytilus galloprovincialis (Lamarck); a pro-survival response. The Journal of Experimental Biology 213: 347-57 Hartwig, A. (2010) Mechanisms in cadmium-induced carcinogenicity: recent insights. Biometals 23 (5): 951-60 Imbra, R.J. and Karin, M., (1987) Metallothionein gene expression is regulated by serum factors and activators of protein kinase C. Molecular and Cellular Biology 7(4): 1358-63 LaRochelle, O., Gagne, V., Charron, J., Soh, J. W. and Seguin, C. (2001) Phosphorylation is involved in the activation of metal-regulatory transcription factor 1 in response to metal ions. Journal of Biological Chemistry 276 (45): 41879-88 Lison, D., Raguzzi, F. and Lauwerys R. (1990) Comparison of the effects of auranofin, heavy metals and retinoids on protein kinase C in vitro and on a protein kinase C mediated responses in macrophages. Pharmacology & Toxicology 67: 239-42 Martin, P., Boulukos, K.E., Poggi, M.C. and Pognonec, P. (2009) Long-term extracellular signal-related kinase activation following cadmium intoxication is negatively regulated by a protein kinase C-dependent pathway affecting cadmium transport. FEBS Journal 276: 1667-79 Mazzei, G.J., Girard, P.R. and Kuo, J.K. (1984) Environmental pollutant Cd2+ biphasically and differentially regulates myosin light chain kinase and phospholipid/Ca2+-dependent protein kinase. FEBS Letters 173 (1): 124-8 Miguel, G.G., Rodriguez, M.E., Aller, P., Martinez, A.M. and Mata, F. (2005) Regulation of cadmium-induced apoptosis by PKC delta in U937 human promonocytic cells. Biochimica et Biophysica Acta 1743: 215-22 Morgan, J. E. and Morgan, A. J. (1998) The distribution and intracellular compartmentation of metals in the endogeic earthworm Aporrectodea caliginosa sampled from an unpolluted and a metal-contaminated site. Environmental Pollution 99: 167-75 Newton, A. C. (1995) Protein kinase C: Seeing two domains Current Biology 5: 973-6 Rajanna, B., Chetty, C.S., Rajanna, S., Hall, E., Fail, S. and Yallapragada, P.R. (1995) Toxicology Letters 81: 197-203 Speizer, L.A., Watson, M.J., Kanter, J.R. and Brunton, L.L. (1988) The Journal of Biological Chemistry 264 (10): 5581-5 Wang, Y., Fang, J., Leonard, S. S. and Rao, K. M. K. (2004) Cadmium inhibits the electron transfer chain and induces reactive oxygen species. Radical Biology & Medicine 36 (11): 1434-43 Watjen, W., Haase, H., Biagioli, M. and Beyersmann, D. (2002) Induction of apoptosis in mammalian cells by cadmium and zinc. Environmental Health Perspectives 110(5): 865-7 Yamasaki, S., Sakata-Sogawa, K., Hasegawa, A., Suzuki, T., Kabu, K., Sato, E., Kurosaki, T., Yamashita, S., Tokunaga, M., Nishida, K. and Hirano, T. (2007) Zinc is a novel intracellular second messenger. Journal of Cell Biology 177 (4): 637-45 Yu, C. W., Chen, J. H. and Lin, L. Y. (1997) Metal-induced metallothionein gene expression can be inactivated by protein kinase C inhibitor. FEBS Letters 420: 69-73 | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/41164 | - |
dc.description.abstract | 許多研究已指出重金屬鎘會累積在生物體內並造成多種病變,因此工業上對於鎘的使用以及其對於環境污染所造成的影響受到了相當的關注。在以蚯蚓Eisenia andrei(安德艾氏蚓)為主的研究上,鎘雖已被認為與MT(metallothionein,金屬硫蛋白)之表現、不正常的移動行為、以及個體的死亡相關,然而截至目前為止並無相關研究指明鎘引起病變之機制為何。本實驗藉由測量PKC(protein kinase C,蛋白質激酶C)酵素活性以及MT之表現,以探討鎘引起相關病變之可能機制。
在酵素活性測試中,鎘離子會導致PKCα的酵素活性受到抑制。當高濃度鈣離子與鎘離子同時加入並無法抑制鎘所導致之酵素活性下降,推測此活性抑制並與PKCα結構中之鈣離子結合位無關。然而由於鋅離子本身即對PKC活性造成抑制,因此此部份的實驗並沒有辦法確實得知鎘離子所導致之活性抑制,是否是由鎘離子與鋅離子競爭PKCα之結合位相關。 除此之外,本實驗亦同時發現鎘與PKC之活化劑phorbol 12-myistate 13-acetate (PMA)可同時引起MT表現以及個體死亡。若考慮鎘會導致PKC活性下降以及MT表現,則鎘離子、PKC以及MT表現三者之間的關係將會產生矛盾。本研究推測在E. andrei中應存在兩種以上之PKC亞型參與本實驗所觀察到的現象:α-like PKC會受到鎘離子之影響而導致其酵素活性下降;而δ-like PKC將受到鎘離子的影響而誘發MT表現以及個體死亡。透過本研究可推論出蚯蚓如何感應以及反應重金屬鎘之毒害的可能機制,但仍需要後續更深入的研究以證明此假說。 | zh_TW |
dc.description.abstract | Cadmium can be accumulated in organisms and cause a variety of pathological responses. The concern of using this heavy metal and its detrimental effects to public health are rising in the past decades. So far, cadmium toxicology researches done with earthworm Eisenia andrei only correlate cadmium with heavy metal-induced responses like expression of metal-inducible metallothionein (MT), abnormal locomotion and death of individuals. However, there is still no explanation of how the organisms sense and respond to cadmium. In this experiment, the protein kinase C (PKC) activities and expression of a metal-inducible MT were measured in order to find out how cadmium interferes with the function of PKC and leads to mortality and MT expression.
It was found that cadmium inhibited PKCα activity in E. andrei, both in vivo and in vitro. This inhibition was not caused by the competition between calcium and cadmium. However, the change of PKCα activity caused by competition between cadmium and zinc was still remained unproved. Furthermore, cadmium-induced responses such as mortality and MT expression were found to be synergistically induced by the PKC activator phorbol 12-myistate 13-acetate (PMA). There was a confliction resulted among cadmium toxicity, PKC activity and MT expression. This confliction may be due to the existence of multiple PKC isoforms. This multiple-isoform hypothesis suggested that an α-like PKC is responsible for the cadmium-induced inhibition of PKC activity; while a δ-like PKC is responsible for the cadmium-induced mortality and expression of MT. Although more studies are required to further prove the existence and function of these putative PKC isoforms, this study still provided a hypothesis of how earthworm senses and deals with the toxicology caused by heavy metal cadmium via isoforms of PKCs. | en |
dc.description.provenance | Made available in DSpace on 2021-06-14T17:21:02Z (GMT). No. of bitstreams: 1 ntu-100-R96b41013-1.pdf: 1015374 bytes, checksum: b86b09d7397893bf14e1fe6d314cd681 (MD5) Previous issue date: 2011 | en |
dc.description.tableofcontents | 致謝 i
中文摘要 ii Abstract iii 目錄 iv 1: Introduction 1 1-1: Cadmium pollution 1 1-2: Cadmium-caused pathologies and possible relation to PKC 2 1-3: Introduction to PKC 2 1-4: Interaction between PKC and heavy metals 3 1-5: Metallothionein as an indicator of heavy metal-induced responses 5 1-6: Eisenia andrei as a model of soil cadmium toxicology researches 6 1-7: Aim of study 8 2: Material and method 9 2-1: Keeping and treatment of earthworms 9 2-2: Atomic absorption spectrum analysis 9 2-3: PKC activity assay 10 2-4: Mortality test 12 2-5: Primer design 12 2-6: mRNA extraction and quantitative PCR 13 2-7: Statistics 14 3: Results 16 3-1: Cadmium accumulation in Eisenia andrei 16 3-2: Effect of PMA on cadmium induced mortality of E. andrei 17 3-3: Effect of cadmium on PKCα activity in E. andrei 18 3-4: Effect of calcium or zinc on cadmium-inhibited PKCα 19 3-5: Effect of cadmium on MT expression 20 3-6: Effect of PMA on cadmium-induced MT expression 21 4: Discussion 23 4-1: Cadmium effect on PKCα activity 23 4-2: Calcium and zinc effect on cadmium-treated PKC 24 4-3: Involvement of a second PKC isoform in cadmium-induced responses 25 4-4: Identity of the type 1 responses-inducing PKC 27 4-5: Using earthworm as a model to study interaction between cadmium and PKCs 28 4-6: Conclusions 29 5: References 31 6: Figures 35 Figure 1: Dosage effect of cadmium accumulation. 35 Figure 2. Time effect of cadmium accumulation. 36 Figure 3. Temporal effect of PMA on PKCα activity. 38 Figure 4. PMA’s effect on cadmium-induced mortality. 39 Figure 5. Dosage effect of in vivo cadmium treatment on PKCα activity. 41 Figure 6. Time effect of in vivo cadmium treatment on PKCα activity. 43 Figure 7. Dosage effect of in vitro cadmium treatment on PKC activity. 45 Figure 8. Calcium effect on cadmium-inhibited PKC activity. 47 Figure 9. Zinc effect on cadmium-inhibited PKC activity. 49 Figure 10. Dosage effect of cadmium on MT expression. 51 Figure 11. Time effect of cadmium on MT expression. 53 Figure 12. PMA’s effect on cadmium-induced MT. 55 Figure 13. Model of cadmium-induced responses. 57 7: Tables 58 Table 1: Sequences of primers used for Polymerase qPCR. 58 Table 2: Classification of cadmium-induced responses 59 | |
dc.language.iso | en | |
dc.title | 重金屬鎘影響安德艾氏蚓蛋白質激酶C活性之研究 | zh_TW |
dc.title | The Study of Cadmium Effect on Eisenia andrei Protein Kinase C | en |
dc.type | Thesis | |
dc.date.schoolyear | 99-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 李心予(Hsin-Yu Lee),廖秀娟 | |
dc.subject.keyword | 鎘,蛋白質激酶,Cα,金屬硫蛋白,PMA,安德艾氏蚓, | zh_TW |
dc.subject.keyword | cadmium,protein kinase Cα,metallothionein,PMA,Eisenia andrei, | en |
dc.relation.page | 59 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2011-08-11 | |
dc.contributor.author-college | 生命科學院 | zh_TW |
dc.contributor.author-dept | 動物學研究所 | zh_TW |
顯示於系所單位: | 動物學研究所 |
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