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http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/41148
標題: | Sulfolobus solfataricus RadA 的晶體結構:
三種新構型及其對同源重組的啟發 Crystal Structure of Sulfolobus solfataricus RadA: Three New Conformations and their Implications in Homologous Recombination |
作者: | Yu-Wei Chang 張育瑋 |
指導教授: | 王惠鈞(Andrew H.-J. Wang) |
關鍵字: | 同源重組,RECA,RADA,斜方晶系晶體結構,左手旋蛋白質絲狀聚合體,蛋白質絲狀聚合體的組合, Homologous recombination,RecA family proteins,RadA,orthorhombic crystal structures,left-handed protein filaments,filament assembly, |
出版年 : | 2008 |
學位: | 碩士 |
摘要: | Homologous recombination is a universal mechanism for repairing DNA double strand breaks (DSBs) and injured DNA replication forks. RecA family proteins play a central role in homologous recombination by forming nucleoprotein filaments. These proteins include the prokaryotic RecA, archaeal RadA, and eukaryotic Rad51 and Dmc1. Here we report three crystal structures of the Sulfolobus solfataricus RadA. All of these structures are packed in the orthorhombic lattice and form the left-handed helical filaments with different helical pitches. The results strongly suggest the universal existence of left-handed helical filaments of the RecA family proteins. The structural variations of alternate protein-protein interfaces not only exhibit the extreme flexibility of filaments but also demonstrate the asymmetry within the filaments, implying the sequential binding of DNA and hydrolysis of ATP by RecA family proteins. Further structural analyses and subsequent mutagenesis studies, coupled with biochemical assays, offer a novel insight into the mechanism controlling the SsoRadA quaternary structures and filament assemblies during the catalysis of strand exchange, which involves Asp 70 and Arg 72 in the N-terminus of the NTD-CTD hinge. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/41148 |
全文授權: | 有償授權 |
顯示於系所單位: | 生化科學研究所 |
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